Objective To observe the effect of traditional chinese medicine(TCM)therapy on improving quality of life of patients with colorectal carcinoma(CRC).Methods Patients with CRC treated at the first affiliated hospital to Guangzhou university of traditional Chinese medicine and the second affiliated hospital of Guangzhou medical college from March 2005 to April 2006 were enrolled.90 valid cases were randomly divided into three groups:TCM group(30 eases),integrated traditional and western medicine group (30 cases)and western medicine group(30 cages).The FACT-C were used to evaluate the quality of life(QOL).Results The QOL wag best in integrated traditional and western medicine group.worst in western medicine group.When the trial finished,TCM group and integrated traditional and western medicine group gained a significant ameliorating effect of QOL compared with pre-treatment scores(P＜0.05).Conclusion The results prompted that TCM have antagonistic effect on the adverse reactions of chemotherapy and Can improve the patients' quality of life in a certain degree.

Objective To investigate the clinical diagnostic and differential diagnostic values of antisense non-coding RNA in the INK4 locus (ANRIL) and tumor suppressors (p14ARF, p15INK4b and p16INK4a) mRNA expression levels in peripheral blood lymphocytes of patients with cirrhosis and hepatocellular carcinoma. Methods The patients with hepatocellular carcinoma and cirrhosis admitted in Shantou Central Hospital from October 2013 to April 2014 were selected. The real-time quantitative reverse transcription-polymerase chain reaction (RT-qPCR) was used to detect ANRIL, p14ARF, p15INK4b and p16INK4a mRNA expression levels of peripheral blood lymphocytes. The subjects having taken physical health examinations in outpatient clinics were assigned in the healthy control group. Results During the study period, 19 cases of hepatocellular carcinoma, 24 cases of cirrhosis, and 31 healthy controls were finally enrolled. In the hepatocellular carcinoma group, the mRNA expression level of ANRIL was significantly higher than that of the healthy control group (?Ct:13.07±0.62 vs. 12.45±0.84, P<0.01), while p15INK4b mRNA expression level was obviously lower than that of the healthy control group (13.24±0.98 vs. 13.99±0.99, P<0.05). But there were no significant differences in the mRNA expression levels of ANRIL (13.07±0.65 vs. 12.71±0.76) and p15INK4b (13.24±0.98 vs. 13.55±1.08) between the groups of hepatocellular carcinoma and cirrhosis (both P>0.05). There were also no statistically significant differences in p14ARF and p16INK4a mRNA expressions among the three groups (all P>0.05). Conclusion The elevation of ANRIL and descent of p15INK4b mRNA expression levels in peripheral blood lymphocytes in patients with liver lesion can be used as the reference indicators for the early diagnosis of hepatocellular carcinoma and to predict their prognoses.

0.05).Conclusion Gatifloxation is safe and efficient in the treatment of community acquired pneomonia.

Objective To Investigate correlation between screening assay of human papillomavirus (HPV) and microbial pathogens in gynecology. Methods Cervical samples were collected to search for HPV, bacteria and yeast infections in gynecologic outpatients. HPV typing was carried out by PCR and sequencing on cervical brush specimens. Chlamydia trachomatis was identified by strand displacement amplification (SDA) and the other microorganisms were detected by conventional methods. All data were analyzed to investigate the correlation among them. Results In this cross-sectional study, among 857 enrolled outpatients, there were 266 cases with positive HPV DNA, and the rate of infection was 31.0%(266/857). HPV genotype showed that thirty-five different HPV types were identified, of which HPV16 was the most prevalent ( 14.5%, 38/262), followed by HPV58 (9.2%, 24/262), HPV53 (8.0%, 21/262)and HPV42 (6.1%, 16/262); while other genotypes were present in less than 5% of HPV positive women.According to the reclassification, the aggregated percentage (high-risk and probably high-risk) of detected HPV was 58.8% ( 154/262), 27.9% (73/262) for low-risk and 13.4% (35/262) for unknown-risk HPV types. Among HPV positive women, cervical brush specimens results showed that more than 60% cases with normal cytology, 3.8% (10/266) with high-grade squamous intraepithelial lesions (HSIL), 29. 7% (79/266) with low-grade squamous intraepithelial lesions (LSIL) and 3.0% (8/266) with atypical squamous cells of undetermined significance (ASCUS), respectively. Statistical analyses revealed there was a significant association between the infected HPV and Chlamydia trachomatis or Ureaplasma urealyticum ( >10000 CCU/ml; all P <0.01), while no correlation was found between HPV infection and bacterial vaginosis, streptococcus agalactiae, candida, Trichomonas vaginalis or Ureaplasma urealyticum ( ≤ 10000CCU/ml; all P > 0.05 ). Among the cases with bacterial vaginosis, the positive rate of HPV infected was42.6%. Chlamydia trachomatis was one of the high-risk factors for the infection of HPV ( OR = 2.82, 95%CI: 1.74 -4.57). Mycoplasma hominis was isolated only in 2 cases, no patient was infected with Neisseria gonorrhocae. Conclusions Although bacterial vaginosis was not significantly associated with HPV, it was more common among the HPV positive women. There is the significant association between HPV and Chlamydia trachomatis or Ureaplasma urealyticum which may be increase the infection of HPV. These data suggest that it may be important to screen for the simultaneous presence of different microorganisms which may have synergistic pathological effects.

Acupuncture Points ; Acupuncture, Ear ; Adult ; Dizziness ; therapy ; Headache ; therapy ; Humans ; Male ; Middle Aged ; Moxibustion ; Rhinitis, Allergic, Seasonal ; therapy

ObjectiveTo observe the clinical effect of sodium aescinate in the treatment of patients with stage Ⅰ - Ⅱ internal hemorrhoids.Methods177 patients with stage Ⅰ - Ⅱ internal hemorrhoids were randomly divided into two groups,the treated group( n =87 ) and the control group( n =90).Patients in the control group were treated with external hemorrhoids suppositories ( 1 per time,bid),while patients in the treated group took sodium aescinate orally(2 per time,Bid).After the 7-day course of treatment,the efficacy of sodium aescinate was determined.ResultsThere was significant difference between the two groups in the total obvious effective rate and the total effective rate(P ＜0.01 ).Compared with the control group,the symptoms of hematochezia and anal pain were more improved in the treated group( P ＜ 0.01 ).There was significant difference between the two groups in the hemorrhoidal mucosa,prolapse,the size of hemorrhoids and total score difference ( P ＜ 0.05 ).The total obvious effective rate and the total effective rate of the treated group were 87.4％ and 95.4％.ConclusionSodium aescinate had better clinical effect in the treatment of patients with stage Ⅰ - Ⅱ internal hemorrhoids.

Aim To study the effect of Rutoside (Ru) on pancreatic acinar cell apoptosis in rats with acute pancreatitis(AP). Methods The AP model in rats was induced by retrograde injection of 3% sodium taurocholate into biliopancreatic duct. Ru (15, 30 and 60 mg?kg~ -1 ?h~ -1 )was administered by intravenous infusion for 6 hours immediately after the induction of AP.The histopathological changes of pancreas were observed under light microscope and electronic microscope.A terminal deoxynucleotidyl transferase-mediated dUTP-biotion nick end labeling(TUNEL) method was used to detect apotosis of pancreatic acinar cell and apoptosis immunohistochemical method. Results Ru (15, 30 and 60 mg?kg~ -1 ?h~ -1 ) improved the histopathological changes of pancreas significantly.The apotosis index of pancreatic acinar cells was significantly higher than that the AP model group. The gray value of Fas was lower than that of the AP model group. Immuno-staining revealed that moderate to strong FasL immunoreactivity was present in the cytoplasm of the acini of all groups.But in AP model group,the gray value of FasL was lower than that in Ru-treated groups.AI was positively correlated with the gray value of Fas,and was negatively correlated with the gray value of FasL and the severity of pancreas damages. Conclusion The protective effect of Ru on AP may be concerned with the induction of apoptosis in injured pancreatic acinar cells.And the Fas/FasL system may contribute to the process.

Aim To investigate effects and the mechanism of rutoside (Ru) on lung injury during experimental acute pancreatitis (AP) . Methods The model of AP was induced by retrograde injection of Sodium Taurocholate(STC)into biliopancreatic duct in rat. Ru (15,30,60 mg?kg -1 ?h -1 ) was administered by intravenous infusion for 6 hours immediately after the induction of AP. Indexes of blood gas analysis, the wet/dry lung ratio and pathological changes of lung tissue were observed and we also measured levels of TNF-?, ICAM-1and NF-?B. Result Ru(60 mg?kg -1 )increased the arterial PO_2, which decreased in AP rats. Ru (30,60 mg?kg -1 ) could decrease the wet/dry lung ratio, and improve pathological changes of lung tissue. Ru(15,30,60 mg?kg -1 ) could decrease levels of TNF-?, ICAM-1, and NF-?B. Conclusion The study indicates that Ru administered by intravenous infusion exerts certain protective effects on lung injury in experimental AP rats by decreasing levels of TNF-?, ICAM-1 and NF-?B.

BACKGROUND:So far the positive or negative effects of mesenchymal stem cel s on tumor growth and metastasis are under discussion. OBJECTIVE:To explore the mechanism of bone marrow mesenchymal cel s in promoting lung cancer metastasis. METHODS:Primary rat bone marrow mesenchymal stem cel s were obtained by direct adherence method of the whole bone marrow, and differential adherence combined with digestion control method was performed to purify cel s. Lung cancer cel lines were cultured, and the effects of bone marrow mesenchymal stem cel s on the migration, invasion and metastasis of lung cancer cel s were observed by scratch test, cel invasion and migration assays. Orthotopic lung cancer models were established in rats and bone marrow mesenchymal stem cel s were seeded onto the left lung of rats. Then, pathological changes of lung tissues were observed at 14 days after transplannation. RESULTS AND CONCLUSION:After the scratch test, the migration rate of lung cancer cel s became higher, and the scratches healed with time. And after cel transplantation, the number of migrated lung cancer cel s increased, as wel as the ability of lung cancer cel s penetrating the Matrigel was strengthened. Besides, fibrous connective tissues could be found around the lung cancer tissues, and necrosis with distinct boundary and large tumor nuclei;the metastatic tissues showed obvious infiltration and necrosis with large tumor nuclei. These results suggest that bone marrow mesenchymal stem cel s can promote the invasion, migration and metastasis of lung cancer cel lines.

Objective To investigate the clinical efficacy of Nazi method of midnight-midday ebb flow plus warm needling moxibustion in treating transient ischemic attack (TIA). Methods Sixty patients with transient ischemic attack were randomly allocated to treatment and control groups, 30 cases each. The control group received routine medication and the treatment group, Nazi method of midnight-midday ebb flow plus warm needling moxibustion in addition. The main clinical symptoms were observed by TCD monitoring of microembolic signals in the two groups before and after treatment. The clinical therapeutic effects were compared between the two groups. Results After treatment, the positive rate of microembolus monitoring was 3.3%in the treatment group and 20.0%in the control group;there was a statistically significant difference between the two groups (P<0.05). There was a statistically significant pre-/post-treatment difference in the traditional Chinese medical syndrome score in the two groups (P<0.05). There was a statistically significant post-treatment difference in the traditional Chinese medical syndrome score between the treatment and control groups (P<0.05). The total efficacy rate was 96.7% in the treatment group and 80.0% in the control group;there was a statistically significant difference between the two groups (P<0.05). At six months after treatment, the rate of disappearance of TIA, the rate of recurrence of TIA and the rate of occurrence of cerebral infarction were 90.0%, 6.7%and 3.3%, respectively, in the treatment group and 60.0%, 20.0%and 20.0%, respectively, in the control group;there were statistically significant differences between the two groups (P<0.05). Conclusions Nazi method of midnight-midday ebb flow plus warm needling moxibustion can decrease the positive rate of microemboli and shorten transient ischemic attack to prevent cerebral infarction in patients with transient ischemic attack.