1.A system for quantitatively measurement of alveolar bone density and the evaluation of its precision and validity
Journal of Peking University(Health Sciences) 2003;0(04):-
Objective:To establish a system for quantitatively measuring alveolar bone density and to test its precision and validity. Methods: With the aid of computer technique, the system measured the average gray level intensity of regions of interest (ROIs) on standardized exposed direct digital periapical radiograph. To correct the variation resulting from exposure condition, an aluminum stepwedge was exposed simultaneously. By referring the image of aluminum stepwedge on each radiograph the equivalent aluminum thickness(EAT)and corrected gray level intensity(CGL)of the ROIs could be calculated as indicators of alveolar bone density. Nineteen specimens containing different amounts of hydroxy-phosphorite were radiographed. The precision of the system was tested by repeatedly measuring 2 of the specimens. The set of specimens was exposed under two different exposure time in order to test the system's validity to correct the gray level difference caused by different exposure time. Results: After repeated measurement of specimens, we found the Relative Standard Deviation(RSD) of EAT and CGL was between 0.83%-2.15%; At the level of 95%, if the difference of EAT between the two ROIs was larger than 0.05 mm or the difference of CGL was larger than 3, the content of hydroxy-phosphorite in them was different; The two sets of data during different exposure time were processed with Wilcoxon Signed Ranks Test, and the result showed effective correction of the variation caused by different exposure time. Conclusion:The precision and validity of the quantitative alveolar bone density measuring system are acceptable. The system can be used to compare alveolar bone density longitudinally and cross-sectionally.
2.Sonographic study on thickness of transverse carpal ligaments in carpal tunnel syndrome patients and healthy volunteers
Orthopedic Journal of China 2006;0(09):-
[Objective]To evaluate the application of sonographic measurements of the thickness of transverse carpal ligaments in CTS(carpal tunnel syndrome) patients.[Methods]Fifty-two wrists from 40 patients with CTS confirmed by surgery and 32 wrists from 20 healthy individuals(control group) underwent sonographic examination of the thickness of transverse carpal ligaments at the level of pisiform bone and the hook of the hamate bone in transverse plane by the same operator.[Results]The thickness of transverse carpal ligaments in CTS patients was(0.41?0.10) cm at hamate hook level and(0.35?0.11) cm at pisiform level versus(0.28?0.11) cm and(0.20?0.08) cm in the control group.There was significant difference in the mean value of thickness of transverse carpal ligaments hetween the two groups at the two levels.[Conclusion]Ultrasonography examination of the thickness of transverse carpal ligaments is a new and valuable technique in the diagnosis of carpal tunnel syndrome and is much helpful to choose operation methods.
3.Structural analysis of spliced variants of hepatitis B virus genomes
Xu LIN ; Dali ZHENG ; Xiao XU
Chinese Journal of Infectious Diseases 2000;0(02):-
Objective To study the structure of spliced variants of hepatitis B virus (HBV) genomes and elucidate their potential pathogenicity. Methods Amplified the spliced variants of hepatitis B virus genomes by mean of PCR from the serum of the patients with chronic hepatitis B, sequenced and analysis the characteristics of such genomes. Results 10 different types of the spliced variants of hepatitis B virus genomes were obtained with the molecular weight ranging from 765 bp to 2039 bp. There were 6 splicing donor sites and 6 accepter sites, respectively in HBV genomes. All spliced variants showed one or more deletions in the regions coding for core, preS1, preS2 and surface protein, while retained thepathogenic X gene and cis elements which were essential for viral replication and packaging. Conclusions Spliced variants of hepatitis B virus genomes were commonly detected in the serum from chronic Hepatitis B patients, the characteristic structure of such variants implied that they might closely co-related with the pathogenicity of HBV.
4.Applied anatomy on transposition of the vascularized periosteal flap with dorsal branch of anterior interossea vessel
Weihong XU ; Heping ZHENG ; Jianhua LIN
Chinese Journal of Microsurgery 2000;0(03):-
Objective The investitgation of vascularized periosteal flap with dorsal branch of anterior interossea vessel was performed to provide anatomical basis for periosteal flap transposition. Methods The courses, branches, distribution and anastomosis of the dorsal branch of anterior interossea vessel were observed on 30 sides of adult cadavers' upper extremities and stimulated operation were perfomed. Results The dorsal branch penetrate out of interosseous membrane of forearm at (6. 6? 1. 4) cm upper the styloid process of the radius. It also sent off branches to the dorsal of ulna and radius that consist the periosteal flap. The dorsal branch send off lateral and medial branch about (2. 6?0. 8) cm upper the styloid process of the radius. The end of the branch anastomoses with dorsal carpal branch of the ulnar and radial vessel' Conclusion The pedicled osteoperiosteal flap can be transferred for repairing the fracture nonunion of ulna and radius and transpose reversely to repair scaphoid and innate bone disease'
5.The practice and thought of cultivating innovative and outstanding medical postgraduates
Hua ZHENG ; Lin XU ; Aiai CHEN
Chinese Journal of Medical Education Research 2015;14(10):1016-1019
The cultivation of medical talents is the main goal of medical postgraduate training. Zunyi Medical University innovates many aspects of postgraduate education, including student sources, spirit of scientific research, curriculum setting, assessment mechanism and quality control, etc. It fo-cuses on cultivating students' practical and innovative abilities through developing professional-typed education, constructing excellent courses, and boosting students' core competitiveness.
6.Clinical value of Rta protein antibody IgG of EB virus in diagnosis for nasopharynx cancer
Qin XU ; Shuihua ZHENG ; Suxiang LIN
International Journal of Laboratory Medicine 2015;(17):2500-2501
Objective To evaluate the clinical value of Rta protein antibody IgG of EB virus(Rta/IgG)in the diagnosis of naso‐pharyngeal carcinoma patients in Zhuhai .Methods A total of 75 patients with nasopharyngeal cancer diagnosed by histopathologi‐cal detection without therapy were recruited into nasopharyngeal cancer group ,100 healthy persons were selected into control group ,there were 19 patients in liver cancer ,gastric cancer ,lung cancer ,lymphoma group and cervical cancer group .Rta/IgG and EB virus antibody of shell (VCA/IgA) were detected by enzyme‐linked immunosorbent assay .Results The positive rate of Rta/IgG in the nasopharyngeal cancer group was significant higher than those of liver cancer ,gastric cancer ,lung cancer ,lymphoma group and cervical cancer and control group(P<0 .05) .The positive rates of Rta/IgG in different clinical stage of nasopharyngeal cancer had no significant differences(P>0 .05) .The sensitivity rates of Rta/IgG single detection ,VCA/IgA single detection ,com‐bined detection of Rta/IgG and VCA/IgA were 81 .3% ,89 .3% ,94 .6% respectively ,while the specificity rates were 93 .0% ,84 . 0% ,80 .0% respectively .Conclusion Rta/IgG is a sensitive and specific serologic parameter for nasopharynx cancer diagnosis ,but it is not a proper maker for evaluating clinical stage of nasopharynx cancer .The combined detection of Rta/IgG and VCA/IgA could improve the diagnosis rate of nasopharyngeal carcinoma in Zhuhai .
7.Proteomics analysis of the apoptotic effects of hepatitis B virus large envelope protein on HepG2 cells
Dali ZHENG ; Qingling HUANG ; Yunli WU ; Jingyin LIN ; Xu LIN
Chinese Journal of Microbiology and Immunology 2010;30(12):1085-1091
Objective To assess the effects of hepatitis B virus(HBV) large envelope protein (LHB) on the apoptosis of HepG2 cells and explore the possible mechanism by proteomic approaches.Methods LHB gene was cloned into pShuttle-IRES-hrGFP-1,and the recombinant adenovirus either barboring LHB(Ad-LHB) or empty vector(Ad-GFP) were separately generated.Annexin V-FITC apoptosis detection kit,JC-1 mitochondrial membrane potential assay kit and propidium iodide(PI) staining kit were employed combined with flow cytometry to detect the apoptotic cells infected with Ad-LHB or control of Ad-GFP.The cellular proteins were collected after infection of HepG2 cells by Ad-LHBs or Ad-GFP,and a total of 600 μg proteins were submitted to two-dimensional gel electrophoresis(2-DE) and stained with R350.The gel images were captured by ImageScanner Ⅱ Imaging System,the differentially expressed proteins were identified by ImageMaster 2D Platinum analysis software and picked up by Ettan Spot Picker.After enzyme digestion,the protein samples were analyzed by MALDI-TOF-TOF MS.Results HepG2 cells infected with Ad-LHB were much more prone to apoptosis.There were thirty nine differentially expressed proteins were determined by 2-DE between HepG2 cells infected with Ad-LHB and Ad-GFP,and they were identified ultimately and categorized into thirty three kinds of proteins by MALDI-TOF-TOF MS.Among these proteins,nine were found to be closely related to cell apoptosis,in which CAPN2,eIF3K and PPP2CB were higher expressed in Ad-LHB infected HepG2 cells,and SERPINH1,LASP1,PRDX1,DHRS2,LDHA and PS-MA4 were lower expressed in Ad-LHB infected HepG2 cells.Conclusion LHB could induce apoptosis of HepG2 cells,and several apoptosis-related proteins participated in this process.
8.Effect of a single dose of propofol at the end of surgery on pediatric anesthesia emergence delirium undergoing adenotonsillectomy during sevoflurane anesthesia
Yanguo ZHENG ; Xiwen WU ; Xianfa LIN ; Jianjun XU
Chinese Journal of Postgraduates of Medicine 2010;33(18):18-20
Objective To investigate the effect of a single dose of propofol at the end of surgery on pediatric anesthesia emergence delirium undergoing adenotonsillectomy during sevoflurane anesthesia.Methods Sixty pediatric patients for adenotonsillectomy were randomly divided into propofol group and control group (30 cases in each group), they were undergoing sevoflurane induction and maintaince. The pediatric patients in propofol group were received 1 mg/kg 10% of the propofol intravenous injection at the end of surgery,while the pediatric patients in control group were received the same volume physiological sailine intravenous injection. Duration of surgery, sevoflurane administration, anesthesia time, extubation time were recorded. The highest PAED score after extubation in 30 min were recorded. The time spent in PACU and the FLACC pain score were recorded. Results The PAED score in propofol group was significantly lower than that in control group [(7.5 ± 3.9) scores vs (10.9 ±4.5) scores, P<0.05]. The incidence rate of postoperative delirium in propofol group was significantly lower than that in control group [20.0% (6/30) vs 46.7% (14/30), P < 0.05 ] . Duration of extubation in propofol group was slightly longer than that in control group[(10.2 ± 1.1 ) min vs (9.1 ± 1 .5 ) min, P < 0.05].There was no statistical significant difference in duration of anesthesia, the time spent in PACU and the FLACC pain score (P >0.05).Conclusions The administration a single dose of 1 mg/kg propofol at the end of surgery can effectively reduce pediatric anesthesia emergence delirium undergoing adenotonsillectomy during sevoflurane anesthesia, while can't extend the duration of anesthesia and the time spent in PACU. It has some clinical value.
9.Development on research of acupuncture effects on microcirculation.
Lijiao LIN ; Jinsen XU ; Xiaoxiang ZHU ; Shuxia ZHENG
Chinese Acupuncture & Moxibustion 2015;35(2):203-208
The Chinese and English published literature regarding the effects of acupuncture on microcirculation in recent years were analyzed, and it was found out that the theory of TCM meridian had subtle connection with microcirculation on structure and function, which could provide a possible breakthrough for the research of meridian essence. Acupuncture, through multiple links, multiple paths and multiple levels, could regulate microcirculation under physiological or pathological condition, which was a bidirectional benign adjustment function, and reflected the characteristic of acupoint specificity. Due to many shortcomings in the current studies, studies with rigorous design, large sample size and multicenter are expected in the further to make a standard systematized research.
Acupuncture Points
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Acupuncture Therapy
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Meridians
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Microcirculation
10.Anatomical basis of lateral antebrachial neurocutaneous flap pedicled with inferior cubital artery perforator
Heping ZHENG ; Chaoyong CHEN ; Hao XU ; Jian LIN ; Fahui ZHANG
Chinese Journal of Microsurgery 2011;34(1):50-52,后插6
Objective To provide anatomical basis for lateral antebrachial neurocutaneous flap pedi-cled with inferior cubital artery perforator in repairing tissue defects around elbow joint. Methods Thirty embalmed upper limbs of adult cadavers perfused with red latex were used for this study, and followings were observed:①The course and distribution of lateral antebrachial cutaneous nerve; ②Anastomoses between inferior cubital artery and nutrient vessels of lateral antebrachial cutaneous nerve. Mimic operation was performed on other side of fresh specimen. Results ①The main trunk of lateral antebrachial cutaneous nerve (LACN) lined in the radial forearm and distributed in the 1/3 region of lateral forearm. ①The nutritional vessels of the flap were plurisegmental and polyphyletic. The inferior cubital artery which was relatively constant reached to skin through "V"-shaped peak formed by communicating branches of cephalic vein and deep venous system. They also gave off large number of small veins, which closely aligned with perineural branches and neural stem vascular chain of lateral antebrachial cutaneous nerve. Conclusion The lateral antebrachial neurocutaneos flap pedicled with inferior cubital artery perforator can be formed to repaire tissue defects around elbow joint.