Training of doctor receiving in-service training is the important part of clinical education in teaching hospital. It will improve the clinical and scientific research ability of the doctor receiving in-service training and will train high-quality medical persons for primary hospital by training before going to post,clinical teaching,surgical operation teaching,standardized special lecture,case report and various kinds of science activities.

Objective To investigate the clinical efficacy and significance of preoperative superselective endovascular embolization in treatment of patients with vascularized meningioma.Methods 3-9 days before operation, 98 patients with vascularized meningioma underwent DSA and preoperative superselective embilization with PVA particles, gelatin sponge, and silk. The surgical intervention was performed after embolization. Results The vascularized meningioma was mainly supplied by the middle meningeal artery, ascending pharyngeal artery, occipital artery, internal maxillary artery as well as submeningeal artery. After the supplying artery was embolized, tumors' staining in 42 cases disappeared completely and that of 56 cases disappeared totally or partly. Most patients were operated on 3-9 days after embolization. 64 tumors were removed completely, while other 34 cases were removed totally or partly. The average volume of bleeding during the operation was 950 ml in the former and 1 500 ml in the latter.Conclusions The best time for operation is 7～9 days after embolization. Preoperative embolization of the vascularized meningioma contributes to reduce the bleeding significantly during the operation, increase the safety of the operation and improve the total removal of the tumor. It is a safe and effective microinvasive method.

20 mm),these three imaging techniques(HAP-MDCT,Lipiodol CT and DSA)had the same sensitivity,for 10~20 mm hepatic nodules,the detecting rate was no significant difference among them,while,for

Objective Abstract:Objective To inquire into the reason that chronic diabetic foot ulcer(CDF) was difficult to be cured by clarifying their interaction between interin-1?(IL-1?) and fibroblast proliferation,the activity and protein expression of matrix metalloproteinase-2(MMP-2),matrix metalloproteinase-9(MMP-9)in fibroblasts from CDF.Method Fibroblast collected from the edge area of the diabetic foot ulcer was cultured for 4-6 generations,and added in with different concentration of IL-1? at the 24 hr,48 hr and 72 hr,then the effect of IL-1? on the activity and expression of MMP-2 and MMP-9 in fibroblasts were observed by methods of zymography and Western blott.Results There was a significant difference of IL-1? between CDF and acute diabetic lesions(ADL);IL-1? in 0.5 ng/ml-5 ng/ml could promote fibroblast proliferation obviously,but weakened gradually with increasing of the IL-1?,but no affection on fibroblast proliferation when IL-1? was in 50 ng/ml,and inhabited fibroblast proliferation over 50 ng/ml of IL-1?;IL-1? in 50 ng/ml increased the activity and protein expression of MMP-2 and MMP-9 in fibroblasts from CDF. Conclusion The reason that CDF was difficult to be cured were related to significant increase of IL-1?.The increase of IL-1? not only inhibits the fibroblast proliferation,but also promotes the activity and protein expression of MMP-2 & MMP-9,and it results in the increase of extracellular matrix degradation.

Objective To develop a new single-tube polymerase chain reaction amplification (ST Amp) protocol for the efficient sequencing-based typing (SBT) of human leukocyte antigen DRB1(HLA-DRB1).Methods A set of 7 group-specific exonic 5′ amplification primers and a single generic 3′ primer were included together in a single PCR mix to facilitate a single PCR amplification per sample for HLA-DRB1 typing.Results All samples were successfully typed, the typing result was accurate and repeatable.Conclusion ST Amp technique has resulted in the ability to perform high-resolution, high-specificity and high-throughput HLA-DRB1 typing by DNA sequencing.

Background and purpose: Oxymatrine, which is the main effective component of Sopkora flavescens Ait, has anti-fibrosis and antiviral activities, and also has a good effect on leukopenia after either chemotherapy or radiotherapy. Recent studies showed that oxymatrine has the abilities of anti-invasion and killing tumor cells in some degree, and as a supplementary anticancer drug in chemical therapy. In this study, we investigated the antitumor mechanism of oxymatrine by observing cell proliferation and VEGF expression in human gastric cancer SGC-7901 cell line. Methods: Human gastric cancer SGC-7901 cells was cultured in vitro and treated with oxymatrine, then cell proliferation was examined by the method of MTT. Immunohistochemistry was applied to examine the protein expression of VEGE The transcriptions of VEGF mRNA were demonstrated by RT-PCR technique.Results: Low-dose (0.5 mg/mL) oxymatrine has a mild inhibitory effect on cellular proliferation of SGC-7901 cells (P>0.05). When concentration exceeded 1 mg/mL, oxymatrine significantly inhibited cellular proliferation in a time-and concentration-dependent manner, and down-regulated the mRNA and protein expression of VEGF (P<0.05).Conclusion: Within a certain drug concentration, oxymatrine can inhibit the proliferation of SGC-7901 cells and play a potential role in inhibiting angiogenesis by down-regulating the expression of VEGF.

Objective To evaluate the effects of repeated sevoflurane anesthesia on long-term cognitive function in lactating rats.Methods Twenty-four healthy Spragne-Dawley rats,aged 7 days,weighing 14-17 g,were randomly divided into 3 groups (n =8 each):control group (group C),2.6 ％ sevoflurane group (group S1),and 1.5％ sevoflurane group (group S2).At 7,14 and 21 days after birth,2.6％ and 1.5％ sevoflurane and carrier gas were inhaled for 1 h in groups S1,S2 and C,respectively.Visible plafform trial was carried out on 28 and 29 days after birth,and the swimming speed of the rats was recorded.Place navigation test was performed on 32-36 days after birth,and the escape latency was recorded.Spatial probe test was carried out on 36 days after birth,and the time spent in the platform quadrant,swimming distance and the number of times crossing the platform quadrant was recorded.Results Compared with group C,the escape latency was significantly prolonged in groups S1 and S2 (P ＜ 0.05).The escape latency was significantly longer in group S1 than in group S2 (P ＜0.05).There were no significant differences between the three groups in the swimming speed,time spent in the platform zone,swimming distance and the number of times crossing the platform quadrant (P ＞ 0.05).Conclusion Repeated inhalation of sevoflurane during lactation can impair the long-term declarative memory,which is concentration-related,while it has no effects on the associative learning in rats.

Objective To compare the quality of emergence from TCI of sufentanil and remifentanil supplementing propofol-sevoflurane anesthesia in patients undergoing radical colo-rectal cancer resection.Methods Forty ASA Ⅰ or Ⅱ patients of both sexes aged 40-64 yr undergoing elective radical colo-rectal cancer resection were allocated into 2 groups ( n =20 each):sufentanil group (group S) and remifentanil group (group R).Anesthesia was induced with propofol TCI at plasma concentration (Cp) of 4.0 μg/ml in both groups and sufentanil TCI (effect-site concentration Ce =0.4 ng/ml ) or remifentanil TCI ( Cp =4.0 ng/ml).Tracheal intubation was facilitated with vecuronium 0.1 mg/kg.The patients were mechanically ventilated (VT =8-10 ml/kg,RR =12-16 bpm).PErCO2 was maintained at 30-40 mm Hg.Anesthesia was maintained with propofol TCI-sevoflurane supplemented with sufentanil (Ce=0.25 ng/ml) or remifentanil (Cp=2.5 ng/ml).The depth of anesthesia was maintained at Narcotrend index of 37-56 by adjusting Cp of propofol TCI and sevoflurane concentration.The infusion of sufentanil was discontinued at 40 min before the conclusion of the operation while remifentanil was administered until the end of surgery.The incidence of postoperative adverse events,the time from the end of operation to eye openg and the time to extubation were recorded.Reesults The two groups were comparable with respect to demographic data.Neither group developed prolonged emergence and respiratory depression but the time from the end of operation to eye opening and the time to extubation were significantly longer in group S than in group R.The incidence of hypertension and tachycardia,agitation,shivering aad coughing were significantly lower in group S than in group R.Conclusion The quality of emergence from sufentanil supplementing propofol-sevoflurane anesthesia is higher than that from remifentanil.

Objective To improve the diagnostic and therapeutic levels for spontaneous spinal epidural hematoma(SSEH) by summarizing the effect of microsurgery and clinical diagnosis of SSEH. Methods The therapeutic efficacy of 21 patients with SSEH undergoing microsurgery from March 1996 to March 2003 was retrospectively analyzed. Results On discharge from hospital, muscle strength of the bilateral lower extremities of 13 patients was restored to grades Ⅲ or Ⅳ, 5 to grade Ⅴ but no change was found in 3 patients. Conclusion Early diagnosis of SSEH and surgical treatment are helpful to restoring the function of the injured spinal cord and decrease of sequelae.

Objective To investigate the effect of sevoflurane on blood pressure in patients with different ages and the optimal concen-tration of sevoflurane. Methods 60 patients underwent selective LC operation with conventional induction and sevoflurane maintenance were divided into three groups:the youth group (34 patients), the middle age group (20 patients) and the older age group (6 patients). The SBP, DBP, MAP, CETsev, number of patients, age of patients before induction and 10 min(T1), 20 min(T2), 30 min (T3), 60 min (T4) after induction were recorded. Results Fluctuate of blood pressure were in the normal range after anesthesia maintenance, and MAP were fluctuated within the range of ± 20% before induction. The age under different CETsev were of no significant difference in each time point (P>0. 05). The age composition ratio in different CETsev were of no significant difference in each time point (P>0. 05). As the time of anesthesia extended, the number of patients under 0% ~1%CETsev decreased from 35 to 11; the number of patients under 1% ~2%CETsev increased from 10 to 20;and the number of patients under 2% ~3%CETsev maintened in 14 approximately. Conclusion The effect of sevoflurane on blood pressure of different ages is approximate. 2% ~3% CETsev is the most optimal concentration during the main-tenance of anesthesia.