The paper collects the network data from www.haodf.com to establish a model,conducts an empirical study on the factors influencing the selection of doctors in online medical websites,and puts forward the suggestions for the construction of online medical websits from 4 aspects of normalizing management,focusing on propaganda,highlighting online public praise,and expanding profit channels.

Objective To study clinical value and significance of thyroid receptor stimulating antibody (TSAb) and thyroid stimulating-blocking antibody (TSBAb) on diagnosis and treatment for children with Graves’disease (GD) and Hashimoto’s thyroiditis (HT). Methods Eighty-eight children with autoimmune thyroid disease (AITD) and treatment time less six months were divided into GD group (n=55) and HT group (n=33). Thirty-eight healthy children were used as control (Normal group). Human serum TSAb ELISA kit and human TSBAb ELISA kit were used to detect the serum TSAb and serum TSBAb in three groups. The serum levels of TSAb and TSBAb were compared between three groups. The correla? tion between TSAb and TSBAb, TSAb/TSBAb and free triiodothyronine three (FT3), free thyroxine (FT4), ultra sensitive thy?roid stimulating hormone (TSH) were analyzed. Results The serum level of TSAb was significantly higher in GD group than that of HT group and Normal group (P<0.05). The serum level of TSBAb was significantly higher in HT group than that of Normal group (P<0.05). There was no significant correlation between serum TSAb and serum TSBAb (P>0.05). There were no significant correlation between serum TSAb, TSBAb and FT3, FT4, TSH (P>0.05). Conclusion TSAb and TSBAb are related to the pathogenesis of GD and HT in children. TSAb and TSBAb have an important clinical value in the diagnosis and treatment for children with GD and HT.

Objective To evaluate the mechanical properties of SEI silicone for maxillofacial prosthesis before and after heat aging and explore its clinical value. Methods Two kinds of silicones, SEI and A-2186, were performed heat aging for 24, 48 and 72 h. The parameters of tear strength, tensile strength, elongation at break and hardness of them were detected under ordinary state and at different time points after heat aging. Statistic comparison and analysis were conducted. Results Tear strength, tensile strength and elongation at break of A-2186 silicone were more favorable than those of SEI silicone under ordinary state, while the parameter of hardness was an exception (P < 0.05). After heat aging and with time progress, tear strength, tensile strength and elongation at break significantly decreased and hardness significantly increased for both A-2186 silicone and SEI silicone (P < 0.05). After heat aging, tear strength and tensile strength of A-2186 silicone were more favorable than those of SEI silicone, while the latter had a minor change in hardness. Conclusion Compared with the worldwide commonly used A-2186 silicone, there is room for improvement in tear strength and tensile strength for SEI silicone. Given the favorable property of minor change in hardness after heat aging, SEI can be used as a prosthetic material for facial soft tissue defect.

Current materials used for maxillofacial prostheses are far from ideal,and how to develop a final prosthetics material is a significant topic in the research of maxillofacial prostheses.In this paper,some pertinent research of silicone elastomeric materials,such as mechanical properties and colorant are reviewed.

Objective To study the construction of the lentiviral-mediated RNA interference(RNAi) vector targering rat suppressors of cytokine signaling 3(SOCS3) gene.Methods Three target sequences were selected by on-line designer software on Ambion according to rat SOCS3 mRNA sequence(NM053565),the complementary DNA contained both sense and antisense oligonucleotides were designed and synthesized.After annealing,these double strands DNA were cloned to pRNA-Lenti-green fluorescent protein(GFP),which contained U6 promoter and GFP.The resulting Lentiviral vector containing SOCS3 shRNA was named pRNA-Lenti-SOCS3-GFP.After the rat glioma cells(C6)were transduced with the constructed 1entiviral vectors,real-time polymerase chain reaction was used to evaluate the level of SOCS3 expression(including siRNA1 group,siRNA2 group,siRNA3 group,vacuity group and siRNA-Negative group).The pRNA-Lenti-SOCS3-GFP and Lentivector Pakaging plasmid mix were cotransfected into 293T to package Lentivirus particles.Culture supematant was harvested,then the virus titer was determined by serial dilution assay.Results The SOCS3 mRNA sequence was successfully cloned to pRNA-Lenti-GFP,which was proved by PCR and DNA sequence.Compared with control group,the SOCS3 mRNA expressions were obviously suppressed in all 3 experimental groups,especially the expression rate in siRNA1 group was reduced by 80%.The Lentiviral particle titer was determined by serial dilution assay with 1.0?1010 TU?L-1.Conclusion The lentiviral-mediated RNAi vector of rat SOCS3 gene has been constructed successfully,this may provide a potential tool for studying and treating SOCS3-related diseases.

Child ; Humans ; Practice Guidelines as Topic ; Sepsis ; therapy ; Shock, Septic ; therapy

Objective To investigate the value of blood culture result in guiding clinical rational use of drugs in clinic.Methods The medical records of 100 children patients with blood culture positive in the four departments of our hospital were retrospectively analyzed.Results The empirical treatment accounted for 98%,the goodness of fit in the medication before culture with the appropriate antibacterial drugs according to final detected bacteria was only 51.6%;after issuing the level 1 report,the adding and substituting drugs in adjusting the antibacterial drugs accounted for about 20% each,60% of antibacterial drugs were not adjusted;after issuing the level 2 report,about 5% of children patients added the antibacterial drugs,the proportion for replacing antibacterial drugs reached 32%,meanwhile the proportion for reducing accounted for 3%;after issuing the level 3 report,the consistency of antibacterial drugs use with the final drug susceptibility test results reached 83%,the inconsistency was only about 5%.Conclusion Clinical pediatric doctors in this hospital still heavily rely on the results of blood culture 3 levels reports,executing antibacterial agents from the experience treatment to the target treatment is also very active.

Objective To explore the effectiveness of long-acting antibacterial material that was used as an intervention in oral care cavity nursing in patients with endotracheal intubation and mechanical ventilation. Methods Patients admitted to Xuzhou Medical College Hospital neurosurgery intensive care unit between 1st December 2013 and 31st August 2014 requiring intubation and mechanical ventilation were enrolled into the study. There were 100 cases randomly selected and were assigned into control group and experimental group by random number table. Each contained 50 cases. Control group used saline for oral care by brushing and irrigation;experimental group used antimicrobial material oral spray in oral care on the top of the method used in control group. The mouth odor, mouth infection rate and ventilator-associated pneumonia (VAP) rate were used to determine. Results About oral odor, mouth infection rate, and VAP rate, the difference of incidences between experimental group and the control group was significant (χ2=4.504-13.728, P <0.05). Conclusions Antimicrobial material can effectively improve oral health in intubated and mechanical ventilated patients. It slows oral acidic environment change, reduces oral infections early onset VAP incidence. And it is worth promoting its use.

To explore the effects of microRNA-150 deletion on the development and homeostasis of regulatory T cells (Treg),γδT cells,NK and NKT cells.Methods:microRNA-150 knockout mice were used and microRNA-150 expression was detected by Real-time PCR.The numbers of Treg ,γδT,NK and NKT cells in the thymus and spleen of normal control and microRNA-150 knockout mice were detected by Flow cytometry.Cell apoptosis was detected by Annexin V staining , and cell proliferation was detected by 5-Bromo-2-deoxyUridine ( Brdu ) incorporation.Results: microRNA-150 deletion did not affect the development and homeostasis of regulatory T cells (Treg) andγδT cells.However,microRNA-150 deletion resulted in a significant reduction of the NK and thymic NKT cell number.In addition, microRNA-150 deleted NK and NKT cells showed an arrested developmental and maturational phenotype with a reduced expression of NK 1.1 and CD122.Moreover , cell apoptosis was significantly increased in microRNA-150 deleted NK and thymic NKT cells ,while a lower cell proliferation rate was shown in the microRNA-150 deleted NK but not NKT cells.Conclusion: CD122 may play an important role in the development and homeostasis of mouse NK and NKT cells regulated by microRNA-150.

0.05). Conclusion The dot-immunogold filtration assay is rapid and simple for performing and reading in the detection of IgG against T.s with high sensitivity and specificity.