Objective To investigate the effect of interferon-alpha (IFN-α) on preventing pancreatic fibrosis and expression of pancreatic α-smooth muscle actin (α-SMA) and collagen type m in experimental rat model induced by diethyldithiocarbamate (DDC). Methods Forty male Wistar rats were randomly divided into three groups: normal control group, fibrotic group(PF group) and IFN-α prevention group(IFN-α group). Pancreatic fibrosis were induced by repeated intraperitoneal DDC injection. IFN-α group were treated with subcutaneous injection of IFN-α in saline, administered daily at the dosage of 105 U. Six weeks later, all the rats were sacrificed, the samples of the pancreatic tissue obtained by biopsy were submitted to histological studies. The expression of α-SMA and collagen type Ⅲ in pancreatic tissues were detected by immunohistochemistry. Results From the 4th week on, the weight of rats in PF group grew slowly, some rats 'weight even decreased, while the weight of rats in IFN-α group kept growing up slowiy, and the difference became significant at the 5th week (306.17±20.89 g vs 279.17±23.58 g, P＜0.05 ). The fibrosis score, Masson stain score, α-SMA and pancreatic collagen type Ⅲ in PF group were 2.679±0.899, 218. 713± 36. 102, 148.971±30. 686 and 88. 142±42.581, respectively, and the corresponding values in PF group were 1.952±0.219, 114.732±24.912, 77.237±9.275 and 59.952±25.498, respectively. The extent of fibrosis in IFN-α group was significantly better than that in PF group (P＜0.05). Conclusions IFN-α may inhibit the extent of fibrosis, decrease the expression of α-SMA and pancreatic collagen type Ⅲ, therefore, it may partially prevent pancreatic fibrosis induced by DDC.

Objective To compare the influence of different doses of dexmedetomidine on the haemodynamic response caused by tracheal intubation during general anesthesia induction in senile hypertension patients .Methods Sixty patients with essential hy‐pertension(EH) undergoing general anesthesia operation ,60-75 years old ,ASAⅠorⅡ ,were randomly divided into the group D1 , D2 and control group(C) ,20 cases in each group .4μg /mL dexmedetomidine in the group D1 and D2 was intravenously pumped at 15 min before anesthesia induction with the doses of 0 .2 ,0 .6 μg/kg respectively and completed within 10 min;while the group C was pumped with sodium chloride injection by the same method .Mean artery pressure (MAP) ,heart rate (HR) and O2 saturation (SpO2 ) were monitored at before medication(T0) ,before induction(T1) ,before intubation(T2) ,at 1 min(T3) ,5 min(T4) after tra‐cheal intubation .Meanwhile plasma norepinephrine(NE) and epinephrine(E) values were detected .Results Compared with before medication ,MAP before induction in the group D2 was significantly decreased (P<0 .05) ,however which in the group D1 and C had no obvious change(P>0.05);HR at 1 min after tracheal intubation in the group D2 was significantly decreased (P<0.05) , while which in the group C and D1 was significantly increased(P<0 .05) .Compared with the group C ,MAP and HR before induc‐tion and tracheal intubation ,at 1 ,5 min after tracheal intubation in the group D2 were significantly decreased(P<0.05) ,SpO2 was significantly decreased only before induction (P<0.01);MAP ,HR and SpO2 at each time points in the group D1 had no significant differences compared with the group C(P>0.05) .Compared with T0 ,the plasma levels of NE and E at T1 in the group D2 were decreased (P<0.01);the plasma levels of NE and E at T3 in the group C and D1 were increased ,while which in the group D2 were decreased (P<0.01) .The plasma levels of NE and E at T1 and T3 in the group D2 were decreased compared with the group C(P<0.01) .Conclusion Intravenous injection of dexmedetomidine can safely inhibit the tracheal intubation caused hemodynamic changes and keep the hemodynamic stabilization during general anaesthesia induction and tracheal intubation period in senile hyper‐tension patients .Furthermore dexmedetomidine 0.6μg/kg can more effectively inhibit the tracheal intubation caused stress reac‐tions than dexmedetomidine 0.2μg/kg .

Purpose To explore the effects of progesterone receptor isoforms A and B ( PR-A,PR-B) on carcinogenesis and progres-sion of ovarian serous cystadenocarcinoma ( OSC) . Methods The expressions of PR-A and PR-B in 52 cases of OSC, 22 cases of o-varian borderline serous cystadenoma ( OBSC) , 22 cases of umbrella of normal fallopian ( UNF) were detected by immunohistochmical Elivision technique. Results The expression of PR-A in OSCs, OBSCs and UNFs were 94. 5%, 94. 5%, and 68. 38%, respective-ly, with there were statistical significance among three groups (P<0. 05). The expression of PR-B in OSCs, OBSCs and UNFs were 100%, 77. 27%, and 40. 38%, respectively, with there were statistical significance among three groups (P<0. 05). The difference of PR-A/PR-B ratio in OSCs, OBSCs and UNFs was statistical significance ( P<0. 05 ) . The expressions of PR-A and PR-B in OSCs were lower, there were statistical significance between the clinical stageⅠ+Ⅱ andⅢ+Ⅳ (P<0. 05), and histological gradeⅠandⅡ. The difference of PR-A/PR-B ratio between the histological gradeⅠandⅡin OSC was statistical significance. There was statistical significance of PR-B between OSCs with lymph metastasis and without lymph metastasis (P<0. 05). Expression of PR-A and PR-B in OSC was positive correlation (P<0. 05). Conclusion With the carcinogenesis and progression of OSC, the expressions of PR-A and PR-B gradually declined, and the downregulation of PR-B is more obvious, may be an important biological sign of malignant transfor-mation in ovarian tissue. The increasing ratio of PR-A to PR-B in OSC may indicate poor differentiation. The relatively higher expres-sion of PR-B may inhibit the lymph metastasis in OSC.

Objective:To study the anatomy of the maxillary first permanent molars. Methods:Macrography was used to observe the morphology of the mesiobuccal root,the degree of abrasion,the morphology of the floor of pulp cavity, the number and type of apical foramen.Vernier was used to measure the length indexes and the distance from the apical foramen to the tip of the root.Clearing technique was used to investigate the root canal systems. Results:26.9% of mesiobuccal root had type III apical foramen,56.25% of mesiobuccal root had two canals,7.5% teeth had three root canals. Conclusions: The maxillary first permanent molar has a high ratio of more than one root canal in mesiobuccal root(63.75%).

Objective To investigate the prevalence of renal insufficiency and its associated factors in type 2 diabetes mellitus with normoalbuminuria using estimated glomerular filtration rate ( eGFR) .Methods We retrospectively analyzed 10-year data of chronic complications in type 2 diabetics in-patient from the Chinese Diabetes Society.eGFR was estimated using the equation from Modification of Diet in Renal Disease(MDRD) study.The clinical characteristics as well as associated factors for low eGFR were analyzed among the normoalbuminuric type 2 diabetic patients.Results A total of 1351 type 2 diabetic patients were included, 755 patients with normoalbuminuria, 466 patients with microalbuminuria and 130 patients with macroalbuminuria respectively.Among the patients, 310 (22.9% ) had low eGFR (GFR <60 ml · min~(-1) · 1.73 m~(-2) ) , 19.7% (149/755) in the patients with normoalbuminuria, 21.9% (102/466) in microalbuminuria and 45.4% ( 59/130 ) in macroalbuminuria Patients with normoalbuminuria and low eGFR suffered more chronic complications than those with normoalbuminuria and normal eGFR, mainly retinopathy, cerebrovascular diseases and sensory neuropathy.Stepwise logistic regression analysis revealed that age ( OR = 1.042, P < 0.001), diabetic duration ( OR = 1.038, P = 0.045), systolic blood pressure (OR = 1.017, P < 0.001) were independently associated with renal impairment among the patients with normoalbuminuria.Body mass index ( OR = 0.868, P < 0.001) and HbAlc (OR =0.898, P =0.021) were also related with renal insufficiency.Conclusion A considerable proportion in type 2 diabetic patients without albuminuria may exist renal impairment, and eGFR estimation could benefit the evaluation of renal function in such patients.

Objective To investigate the relationship between stent thrombosis and obstructive sleep apnea-hypopnea syndrome (OSAHS) in patients after coronary artery stent implantation (CASI). Methods One hundred and seventeen patients were rolled after CASI, of which 29 suffering from OSAHS were enrolled into the research group, 88 patients without OSAHS were enrolled into the control group. The incidence rate of stent thrombosis between the above two groups was analyzed. Results Three patients suffered stent thrombosis in the research group, with the incidence rate of 10.3%,which was significantly higher than that in the control group, with the incidence rate of 1.10%(P = 0.047). Conclusions After CASI, patients with OSAHS had higher risk of stent thrombosis compared with patients without OSAHS. OSHAS should be considered as a risk factor for stent thrombosis.

Objective To identify snoRNA, which may be related to prognosis of gastric cancer. Methods Ninetygastric cancer patients who diagnosed at Tianjin Medical University Cancer Institute and Hospital were randomly collected in this study, and their clinical data were followed up. A total of 405 snoRNA expression profiles were analyzed in 90 gastric cancer patients. Patients were classified aslow expressiongroup orhigh expressiongroup according to the median expression of each snoRNA expression, which was calculated by univariate and multivariate survival analysis. We also screened out the snoRNAs, in which patients were survived differently. Patients were classified as high, middle, or low risk groups based on the snoRNA risk score. Values of age, gender, smoking, drinking, histological differentiation (well, moderately-differentiated and poorly differentiated), clinical stage (Ⅰ+Ⅱstage andⅢ+Ⅳstage), tumor size (<5 cm and≥5 cm), tumor location (upper 1/3 and others) and snoRNA risk score (high, middle, and low risk group) were assessed by multivariate Cox analysis. Results There were significant differences in overall survival and (or) progression-free survival rates in 19 patients with high and low snoRNAs expressions (P<0.05). Results of multivariate Cox analysis showed that patients with high expression of ACA61,ACA27 and U36A showed a higher overall survival and progression-free survival rates, while patients with high expression of ENSG00000206898 showed a lower overall survival and progression-free survival survival rates (P<0.01). SnoRNA risk score is an independent prognostic factor for patients with gastric cancer. Compared with low risk group, patients in middle risk group and in high risk group showed a shorter overall survival and progression-free survival rates (P<0.001). Conclusion The expressions of ACA61, ACA27, U36A and ENSG00000206898 are independent prognostic factors of gastric cancer. Low expressions of the first three indexes and high expressions of the last one predict a bad prognosis.

AIM: To observe the effect of Shenmai injection,a chinese medicine,on apoptosis of cardiac myocytes after hypoxia.METHODS: Cardiac myocytes were separated from neonate rat heart and cultivated in vitro.Hypoxia condition was induced by mixture of 95%N2 and 5%CO2.Cells were exposed to hypoxia for 6 h or 12 h and treated with Shenmai injection(5 mL/L) from 24 h before hypoxia until the end of hypoxia.First,apoptosis was detected with Annexin V-FITC and PI staining by flowcytometry.Then,the activity of cardiac myocyte mitochondria was observed by MTT method.Mitochondria membrane potential and the activity of caspase 3,7 were also measured by laser scan microscopy and multi-detection microplate reader,respectively.RESULTS: The apoptotic cells became more and more with prolonged hypoxia.Shenmai injection enhanced mitochondria activity,kept membrane potential,inhibited the activation of caspase3,7 and then decreased apoptotic cells(P

AIM: To study the effect of Tribulus terrestris L. saponin (TTLS) on apoptosis and changes in cytosolic calcium concentration induced by hypoxia/re-oxygenation in rat cortical neurons. METHODS: Rat cortical neurons in primary culture were used, and a apoptosis model was induced by hypoxia/reoxygenation. LDH releasing rate was detected by spectrophotometry. The apoptosis rate of cortical neurons was analyzed quantitatively by flow cytometry with Annexin V-FITC and PI staining. Intracellular free Ca2+([Ca2+]i) was observed with a confocal laser-scanning microscope and determined by mean fluorescent value with Fluo-3 fluometry. RESULTS: Compared to control group, three hours of hypoxia and twelve hours of reoxygenation group induced cortical neuronal apoptosis and significantly increased the intracellular free Ca2+ concentration(P