ObjectiveTo observe the clinical efficacy of microinvasive thread embedding plusShe Gan Ma Huangdecoction based on ordinary Western medications in treating chronic obstructive pulmonary disease (COPD) (cold-phlegm obstructing the lung). MethodThe treatment group was intervened by microinvasive thread embedding plusShe Gan Ma Huangdecoction based on ordinary Western medications, while the control group was by the Western medications alone.ResultThe markedly effective rate and total effective rate were respectively 10.0% and 93.3% in the treatment group;the pulmonary function showed significant improvements in both groups.ConclusionMicroinvasive thread embedding plusShe Gan Ma Huangdecoction based on Western medications can produce a more significant therapeutic efficacy than dry Western medicationsin treating COPD (cold-phlegm obstructing the lung) in acute attack stage, and it can simultaneously improve the pulmonary function.

Increased research and exploitation of the low-temperature enzymes of antarctic microorganisms have occurred over recent years. There are a lot of study on protease, lipase and amylase, and so on. In antarctic microorganisms while some peculiar enzymes of antarctic microorganisms are still less researched. The recent development of the peculiar enzymes of the antarctic microorganisms was reviewed in this article. The properties of chitinase, dehydrogenas,?-galactosidase,RNA polymerase,DEAD-box RNA Helicase and metalloprotease etc. were introduced in detail, and the prospects of their application were also discussed.

Objective To evaluate the clinical significance of K-ras mutation in plasma cell-free DNA from patients with colorec-tal carcinoma .Methods Plasma cell-free DNA in 212 patients with colorectal carcinoma were extracted and identified by Globin gene .K-ras mutation was amplified by RE-PCR and compared with results of pathological biopsy samples DNA .Results Plasma cell-free DNA were successfully obtained from 212 cases .But no plasma cell-free DNA were found in normal control .Out of 212 ca-ses ,34 cases showed K-ras mutation(16 .00% )in cell-free DNA ,and 33 cases showed positive in pathological biopsy samples DNA (15 .57% ) .It was found two kinds of K-ras mutation on code 12-GGT to GAT ;GGT to GTT .K-ras mutation was not related to tumors′position(χ2 =3 .34 ,P=0 .61);to differentiation degree(χ2 =2 .38 ,P=0 .29);to Dukes staging(χ2 = 2 .84 ,P=0 .38);to lymphoid nodes′metastasis(χ2 =0 .98 ,P=0 .37);to age(χ2 =1 .86 ,P=0 .39) .Conclusion Tumor-derived DNA can be detected in plasma of underlying cancer patients .For colorectal carcinoma patients ,detecting K-ras mutation of plasma cell-free DNA have the same clinical significance as biopsy samples .Moreover ,it′s more convenient and non-invasive .

OBJECTIVE: There are some people in population susceptible to acute mountain sickness. Therefore, it is important to analyse, assess and integrate certain research results to build prediction system and mathematics model in order to predict those susceptible people when army goes to tableland.DATA SOURCES: Computer was used to search databases such as Medline, PubMed and PML to find articles regarding prediction of people susceptible to acute mountain sickness from January 1970 to December 2002 with the search words "acute mountain sickness, susceptible population,prediction" . The language was limited to English. At the same time, Chinese Journal Full-text Database, Chinainfo and CBMdisc were searched to find articles from January 1970 to August 2004 by the Chinese language of "acute mountain sickness, susceptible population and prediction". The research targets are susceptible people to acute mountain sickness. At last,trace-back method was used to supplement some literature and monographs.DATA SELECTION: After systematically analyzed and concluded the literature information and screened researches without conducting experiment, full text of the rest literature was searched and used as selective criteria if it could be used as prediction indicator of susceptible people to acute mountain sickness.DATA EXTRACTION: Totally there were 19 prediction indicators generalized. After conducting systemic analysis, Delphi method and analytic hierarchy process(AHP) to the indicators, 13 were selected while the other 6 were excluded.DATA SYNTHESIS: The indicators were classified according to nervous-humoral regulation ability, oxygen capture ability of respiratory system,anti-anoxia ability of central nervous system, mental health and health service ability. Based on these, system analysis, Delphi method and AHP method were used to screen prediction indicators, establish indictor system, confirm the weighted indicators and weighted indicator system as well as standardize the prediction indicators.CONCLUSION: According to the principle of prediction model and model methods, weighted linear synthesis method was used to build the predictive mathematic model. The project of susceptible population prediction of acute mountain sickness in army when entering tableland urgently was proposed combining the health service ability of army.

Objective To investigate the association of the SNP in miRNA146A with genetic prediposion and earlier recurrence after resection for hepatocellular carcinoma (HCC).Methods In the casecontrol study including 173 HCC cases,DNA were exacted from cancer tissue embedded with paraffin and were amplificated by PCR,SNP was explored in gene sequence of miRNA146a (385 base pair including extron).The outcome were analyzed with genetic prediposion and clinical features.Result Only hsa-mir-146a rs2910164 was found.The genetype frequence of C/C 、G/G and C/G at rs2910164 gene locus were separately 61 (35.3％),21 (15％) and 86(49.7％) in cases.Compared to G/G genetype,C/C and C/G genetype were danger factor to onset risk of HCC (OR =3.086,95％ CI:1.289-7.390) ; C/G was danger factor to earlier recurrence after resection(OR =8.179,95％ CI:2.248-29.759).Conclusion rs2910164 may be associated with genetic prediposion and earlier recurrence after resection of HCC in Jiangxi hans

Spinal cord injury(SCI) is a serious nervous system trauma,which may consequently lead to various degrees of paralysis and toilet obstacles,and its disability and the high cost heavily burdened the families and community.Therefore,the research on spinal cord tissue regeneration and the repair after injury has important practical significance.A large number of experimental studies have shown that neurotrophic factor plays an important role in the nerve tissue repair process.This article reviews new research progress about the effects of neurotrophic factor in this field.

An innovative approach,named associating liver partition and portal vein ligation for staged hepatectomy (ALPPS),has made possible a marked increase in future liver remnant (FLR) over a short period of time,and has challenged the concept of unresectability.To date,ALPPS has been employed for a variety of primary and metastatic liver tumors from many centers across the world.Many technical variations of ALPPS have been described.However,much controversy has surrounded this procedure,and many problems remain to be solved,such as the reasons for rapid hypertrophy,the technical feasibility,safety and long-term oncologic outcomes,and the result compared with the conventional two-stage hepatectomy.These aspects are needed to be evaluated further in large-scale studies.We believe that the ALPPS procedure will has much room for further development.

Objective To understand the current research status of sentinel lymph node (SLN) biopsy in colorectal cancer. Methods Literatures about the application of SLN biopsy in the field of colorectal surgery were collected and reviewed.Results The results of SLNs biopsy accurately reflected the status of the nodal basin. Focused examination of the SLNs could identify micrometastases that might otherwise had been missed by standard histopathological analysis, thus upstaged this group of patients.Conclusion SLN biopsy represents a new and effective technique to predict the tumor status of regional lymph nodes, which offers a potential alternative to improve the accuracy of tumor staging in colorectal cancer.

Rat lens aldose reductase (AR) activity was assayed in this study with a fiuorometric method. Rat lenses were homogenized in Na, K-phosphate buffer and centrifuged at 19127 ? g, 4℃ for 15 min. The supernatant was reserved as enzyme fraction. The incubation mixture contained 135 mmol KHaPO4-Na2HPO4 buffer (pH 7.0), 100 mmol Li2SO4, 6 ?mol NADPH, 1.0 mmol DL-glyceraldehyde or 10 mmol glucose and 20/ul enzyme fraction. AR activity in diabetic rat lent have not been shown to be significantly higher than that of the con rols. This method can be used properly to screen AR inhibitors in vitro.

Objective To evaluate the changes of hypersplenism patients with post-hepatitis cirrhosis and hypertension who treated by partal splenic embolization.Methods 24 patients with hypertension and hypersplenism were treated by PSE.In the operations we measured the free portal pressure(FPP).Before and after the operation,all patients diameter and velocity of portal veins and splenic veins were measured by Doppler ultrasonography.Results Compared with the condition before operation,the FPP decreased 3.2?0.4 mmHg,the quantity of blood flow of portal veins from 1104 ml/min dropped to 793 ml/min,and that of splenic veins from 897 ml/min dropped to 355 ml/min.Conclusion PSE can improve the state of hyperdynamic circulation of portal veins system in a little time.