Acupuncture Points ; Acupuncture Therapy ; Adult ; Esophageal Achalasia ; therapy ; Humans ; Male

Objective To investigate the effect of aminoguanidine and tienam on bacterial translocation in mice with acute necrotizing pancreatitis (ANP) and the preventive and curative effect on pancreatic infection. Methods 50 SD mice were randomly divided into 5 groups, including group A: normal control (n=10), group B: ANP group (n=10), group C: aminoguanidine treatment group (n=10), group D: tienam treatment group (n=10), and group E: aminoguanidine plus tienam treatment group (n=10). ANP was induced by intrapancreatic injection of 5% sodium tanrocholate (2.5 ml/kg). Aminoguanidine (100 mg/kg) was injected intraperitioneally 30 minutes after ANP induction, tienam (60 mg/kg) was injected intraperitioneally 6 hours after ANP induction. The mice were killed at 48 hours, and serum amylase, serum D- lactate, pancreatic MPO were measured. Pathological alterations in the pancreas were examed. The pancreas, liver, blood, mesenteric lymph node and ascites were collected for microbiological study. Results In group C and E, the levels of serum amylase were 91173.30±199.73) U/L and (1075.00±200.40) U/L; the serum D-lactate were (7.165±1.2533) μg/ml and (6.980±1.060)μg./ml; the pancreatic MPO were (0.8035±0.0711) U/g wet film and (0.7765±0.0843 ) U/g wet film; the average bacterial positive rates were 20% and 16%. In group B, the serum amylase was (2234.60±692.06 )U/L;the serum D-lactate was (12.408±1.779)μg,/ml, the pancreatic MPO was (1.5942±0.1965) U/g wet film; the average bacterial positive rate were 60%. The differences between group C, E and group B were statistically significant (P＜ 0.05). In group D, the pancreatic MPO was (0.8002±0.0603 ) U/g wet film and the the average bacterial positive rate was 18%, and they were statistically different when compared with those of group B (P±0.05). While in group D, the serum amylase and D-lactate was not statistically different when compared with those of group B (P＞0.05). There were lamellar necrosis in the parenchyma, hyperaemia and leukocytic infiltrate in the pancreatic mesenchyma in the ANP group, while no leukocytic infiltrating in group C, D, E was found. Conclusions The aminoguanidine and tienam might decrease gut bacterial translocation and prevent pancreatic infection in ANP.

The evaluation of biomaterial biocompatibility is a key step before the clinical application.With the rapid development of molecular biology,scientists have begun to evaluate biomaterial biocompatibility at molecular level and proposed the concept of molecular biocompatibility.Researchers'main tasks at present are to identify more molecular markers using molecular biology technology and to establish standards for evaluating molecular biocompatibility of biomaterial,which will provide the guidance for the design of better biomaterials.

β-catenin is an important signaling transduetion and adhesion molecules.Mutation of the betacatenin gene,CTNNB1,is a common case in pediatric tumors,which may induce development and metastasis.In hepatoblastoma,48%have CTNNB1 mutation.In Wilms tumor,mutation only occurs in cases with WT1 gene mutation.In neuroblastoma,mutation is also found.

Objective To evaluate the effects of Lactobacillus helveticus fermented milk on blood pressure and heart rate was evaluated in 8w old spontaneously hypertensive rats(SHR).Method Spontaneously hypertensive male rats were randomized into 5 groups(n=8) receiving fermented milk and its whey,water,skim milk and yogurt.Every sample was used to SHR on consecutive three days,during which they received increasing doses by ig.The systolic blood pressure and heart rate were measured by ZH-HX-Z tail cuff with a programmed electro-sphygmomanometer.Results At 1.5 ml dose of Lactobacillus helveticus TUST005 fermented milk and the 2 ml dose of Lactobacillus helveticus TUST005 fermented milk whey,we observed the marked and long-lasting hypertensive effect for 4 and 6 h,respectively.The maximum decrease in mean arterial blood pressure(20.5 mmHg) was achieved.Feeding 1 ml whey of Lactobacillus helveticus TUST005 fermented milk elicited significant decrease in heart rate(maximum of 50 bpm).Conclusion Significant reductions in mean systolic blood pressure,and heart rate were observed following intra-gastric administration of Lactobacillus helveticus TUST005 fermented milk.

Objective:To develop a succinct and effective social support questionnaire for Chinese Farmers.Methods:Based on the studies of Vender Poel Mart et al and combined with the interview data of 53 farmers,a pro-questionnaire was developed;An item analysis was conducted with a sample of 800 farmers of Zhejiang province;CFA was used to test another sample of 807 farmers from Zhejiang and other provinces.Reliability and validity were also tested.Results:Then,9-item questionnaire was formed;EFA indicated that farmers' social support consisted of four factors,namely:instrumental support,company support,emotional support and the utilization degree of social support,which explained 79.23% of total variance;CFA showed the 4-factor model was well fitted;The Cronbaeh's ? of the questionnaire was 0.72 and the split-half coefficient was 0.64;The correlations between 4 factors were 0.10-0.30,and all factor-total correlations were over 0.50.Conclusion:The structure,reliability and validity of Chinese Farmers' Social Support Questionnaire meets the need of psychometrics.

Objective: To explore the influence of social support on Chinese farmers’ career choice consideration. Methods: Based on depth interviews and the pre-test on 419 farmers, a questionnaire of self-efficacy, choice consideration and social support related to occupations representing Holland's six RIASEC career types was developed. With a formal sample of 628 farmers, and by the structural equation modeling, the hypothetic models were constructed and tested. Results: Across most of the career types, there were significant paths between positive support and self-efficacy, self-efficacy and choice consideration, negative support and choice consideration; the goodness of fit of the final models were perfect. Self-efficacy fully mediated the relationship between positive support and choice consideration. Conclusion: The influence of social support on farmers' career consideration is ambilateral. Positive support has fully positive effect on choice consideration via self-efficacy, whereas negative support has direct negative effect on it.

Objective To discuss incidence and related risk factors of vascular cognitive impairment (VCI) after acute subcortical infarction (SI).Methods One hundred and eighty-two patients with acute SI were divided into two groups:VCI group (81 cases) and no-VCI group ( 101 cases) according to VCI diagnostic standard.Sex proportion,age,culture degree between two groups were compared,and correlation was analyzed between the quantity of risk factors and the incidence of VCI.Results The incidence of VCI after acute SI was 44.5％(81/182).According to age stratification,the incidence of VCI in the patients of 56-65 years was 35.4％ (23/65),in the patients of 66-75 years was 45.3％ (48/106),and in the patients of 76-85 years was 90.9％ (10/11 ).The age in VCI group was significantly higher than that in no-VCI group [(69.93 ±6.91) years vs. (67.62 ±5.56) years,P =0.014],and culture degree was significantly lower than that in no-VCI group (P =0.028).The sex proportion between two groups had no significant difference (P =0.876).The more accompanying risk factors,the higher incidence of VCI.Conclusions Along with the increase of age,the incidence of VCI after acute SI is higher.The more accompanying risk factors,the higher incidence of VCI.

Objective To compare the treatment effects of catheter directed thrombolysis and thrombetomy for acute ar?terial ischemia of the lower extremity. Methods Patients who visited Tianjin Medical University General Hospital from 2011 to 2013 and were attacked by acute ischemic disease of lower extremities within 3 days (n=66) were investigated. Thrombetomy and thrombolysis treatment had been performed to 32 cases and 34 cases respectively according to patients’ own willingness. Postoperative symptom remission rate, limb salvage rate, fatality rate, reperfusion injury rate (including re?nal inadequacy, heart failure, muscle fascia compartment syndrome), bleeding rate and limb dysfunction rate were compared between these two groups. Results There was no statistical difference on the rate of symptom remission (93.8% vs 94.1%), limb salvage(96.9%vs 100%), case fatality rate(3.1%vs 0), bleeding rate(0 vs 8.8%)and limb dysfunction rate (0 vs 5.9%)between these two groups. However, the incidence of reperfusion injury was higher in the group of thrombectomy (37.5%vs 2.9%, P<0.05)compared to that of the other group. Conclusion Similar effect of treatment and less reperfusion injury, especially renal injury, had been achieved by CDT compared with thrombetomy.