Acupuncture Points ; Acupuncture Therapy ; Adult ; Esophageal Achalasia ; therapy ; Humans ; Male

Objective To discuss incidence and related risk factors of vascular cognitive impairment (VCI) after acute subcortical infarction (SI).Methods One hundred and eighty-two patients with acute SI were divided into two groups:VCI group (81 cases) and no-VCI group ( 101 cases) according to VCI diagnostic standard.Sex proportion,age,culture degree between two groups were compared,and correlation was analyzed between the quantity of risk factors and the incidence of VCI.Results The incidence of VCI after acute SI was 44.5％(81/182).According to age stratification,the incidence of VCI in the patients of 56-65 years was 35.4％ (23/65),in the patients of 66-75 years was 45.3％ (48/106),and in the patients of 76-85 years was 90.9％ (10/11 ).The age in VCI group was significantly higher than that in no-VCI group [(69.93 ±6.91) years vs. (67.62 ±5.56) years,P =0.014],and culture degree was significantly lower than that in no-VCI group (P =0.028).The sex proportion between two groups had no significant difference (P =0.876).The more accompanying risk factors,the higher incidence of VCI.Conclusions Along with the increase of age,the incidence of VCI after acute SI is higher.The more accompanying risk factors,the higher incidence of VCI.

Objective To evaluate the effects of Lactobacillus helveticus fermented milk on blood pressure and heart rate was evaluated in 8w old spontaneously hypertensive rats(SHR).Method Spontaneously hypertensive male rats were randomized into 5 groups(n=8) receiving fermented milk and its whey,water,skim milk and yogurt.Every sample was used to SHR on consecutive three days,during which they received increasing doses by ig.The systolic blood pressure and heart rate were measured by ZH-HX-Z tail cuff with a programmed electro-sphygmomanometer.Results At 1.5 ml dose of Lactobacillus helveticus TUST005 fermented milk and the 2 ml dose of Lactobacillus helveticus TUST005 fermented milk whey,we observed the marked and long-lasting hypertensive effect for 4 and 6 h,respectively.The maximum decrease in mean arterial blood pressure(20.5 mmHg) was achieved.Feeding 1 ml whey of Lactobacillus helveticus TUST005 fermented milk elicited significant decrease in heart rate(maximum of 50 bpm).Conclusion Significant reductions in mean systolic blood pressure,and heart rate were observed following intra-gastric administration of Lactobacillus helveticus TUST005 fermented milk.

Objectives To establish a sensitive and special method for the detection of Ureaplasma urealyticum(Uu) using PCR microplate hybridization (PCR MPH). Methods A primer of ureasea gene was labeled by biotin. The amplification product was captured on streptavidin coated microplates, then products were quantified by hybridization with a digoxigenin labeled internal oligonucleotide probe. After revelation with an anti digoxigenin alkaline phosphatase coupled antibody(anti DIG AP), the amount was determined by optical reading. At the same time, PCR MPH was compared with Bio Merieux Mycosplasma IST. Results A method of PCR MPH for detecting Uu DNA was established. The morbidity among three groups for detecting 158 clinical samples was analysed. 65 were detected by PCR MPH and 56 by culture.Conclusion The results showed that this assay is rapid, sensitive, specific, and accurate, and is of value in clinical therapy.

Dimethyltrilobine iodide (DMT) 1.5 M-M increased the contractile force of isolated guinea pig left atria. This positive inotropic action could not be an-tagonized by either propranolol, cimetidine or diphen-hydramine. DMT 1.5 nM also increased the contractile force of isolated guinea pig left atria exposed to low concentration of calcium or to verapamil.

Wireless Local Area Networks has been used increasingly in recent years. The technology is mainly used in a-cademia (such as campus)、medical areas 、 manufacturing 、 Storage industry 、 public area、serving industry ,and so on. And the related technologies are making progress all the time. This article analyzes the development of related standard 、the structures 、security and other descriptions of Wireless Local Area Networks, paying attention to the uses of the technology in medical areas .

OBJECTIVE: Apolipoprotein (Apo) E- ε 4 is the first identified important susceptible gene of late-onset family and sporadic Alzheimer disease. Apo E E4 or ε 4 chained other genetic products probably play a certain actions in occurrence of Alzheimer disease, but their biological basis is unknown.To explain the relevant biological mechanism between ApoE and Alzheimer disease is still the key in next study.DATA SOURCE: Computer Medline database is used to search the relevant papers from January 1978 to January 1999, with the words of "develop", "ApoE" and "Alzheimer", limited in English version. Simultaneously, the relevant papers are looked up from China Journal Databasefrom January 1978 to December 2003, with the words of "ApoE, Alzheimer disease and development", limited in Chinese version.STUDY SELECTION: Preliminary examination is done to the data. Inclusive criteria: relevant papers on ApoE gene and Alzheimer disease, including the study of both animal experiment and clinical basic research.Exclusive criteria: repeated study, summarized and Meta analysis papers.DATA EXTRACTION: Totally 55 relevant papers are collected, of which, 20 papers are included and 33 papers are excluded due to repeated experiment and summarized papers.DATA SYNTHESIS: Of 20 experiments, 16 experiments explain that ApoE E4 or ε 4 chained other genetic products probably play a certain actions in occurrence of Alzheimer disease and 4 researches propose that ApoE- ε 4 is the susceptible gene of Alzheimer disease.CONCLUSION: It is viewed in majority at present that ApoE genotype determination cannot substitute any clinical and imaging diagnostic method, which is probably onlya kind of assistant diagnosis to improve the specialty in clinical diagnosis. Beingthe susceptible gene of Alzheimer disease, ApoE- ε 4 is the important discovery in its research no doubt, but,many relevant questions are still at experimental and hypothesis stages. In the future, the great consideration should be still drawn on ApoE genotype determination of Alzheimer disease.

Objective To compare the treatment effects of catheter directed thrombolysis and thrombetomy for acute ar?terial ischemia of the lower extremity. Methods Patients who visited Tianjin Medical University General Hospital from 2011 to 2013 and were attacked by acute ischemic disease of lower extremities within 3 days (n=66) were investigated. Thrombetomy and thrombolysis treatment had been performed to 32 cases and 34 cases respectively according to patients’ own willingness. Postoperative symptom remission rate, limb salvage rate, fatality rate, reperfusion injury rate (including re?nal inadequacy, heart failure, muscle fascia compartment syndrome), bleeding rate and limb dysfunction rate were compared between these two groups. Results There was no statistical difference on the rate of symptom remission (93.8% vs 94.1%), limb salvage(96.9%vs 100%), case fatality rate(3.1%vs 0), bleeding rate(0 vs 8.8%)and limb dysfunction rate (0 vs 5.9%)between these two groups. However, the incidence of reperfusion injury was higher in the group of thrombectomy (37.5%vs 2.9%, P<0.05)compared to that of the other group. Conclusion Similar effect of treatment and less reperfusion injury, especially renal injury, had been achieved by CDT compared with thrombetomy.

Objective To investigate the incidence of drug resistance among new and retreatment TB patients from special hospital.Methods Totally 500 smear positive TB patients from June 2013 to December 2014 in Beijing Chest Hospital were enrolled.Phenotypic susceptibilities to cultured isolates were analyzed in 15 anti tuberculosis drugs by MGIT 960,include Isoniazid (INH),Rifampicin (RFP),Streptomycin (STR),Ethambuto (EMB),Kanamycin (Km),Amikacin (Am),Capreomycin (Cm),Ofloxacin (Ofx),Levofloxacin (Lfx),Moxifloxacin (Mfx),Paza-aminosalicylate (PAS),Protionamide (Pto),Linezolid (Lzd),Ethionamide (Eto),Pyrazinamide (PZA).Results A total of 500 TB cases were enrolled.71 samples among these were NTM infection and 12 samples were contaminted.The rest of 417 of cases infected with Mycobacteriuma,and the rate of drug resistance was 47.2％ (192/417) and the MDR rate was 28.2％ (120/417).The retreatment was significantly higher than that of the new in any drug resistance rate and MDR rate (P =0.000).100 of the retreatment isolates and 50 of the new isolates with Mycobacteriuma were selected to do the drug susceptibility test in 11 subsequent anti tuberculosis drugs (include:PZA,Am,Km,Cm,Ofx,Lfx,Mfx,PAS,Pto,Lzd,Eto).Five cases were contaminated,and in the rest of the cases,48 was the new and 97was the retreatment.The rate of the drug resistance to PZA,Am,Km,Cm,Ofx,Lfx,Mfx for the retreatment were significantly higher than the new (P ＜ 0.05).The rate of drug resistance to PAS,Pto,Lzd and Eto for the new and reteartment were no markedly differential in statistics.Conclusion This study further confirmed the rate of drug resistance in retreatment cases is higher,and the management of tuberculosis patients should be further strengthened.