ObjectiveTo understand basically the attitudes of undergraduates toward disabled persons. MethodsScale of Attitudes toward Disabled Persons edited by Antonak was used to analyze the factor structure of attitudes of undergraduates toward disabled persons with 650 undergraduates as sample. ResultsThere was significant difference in the factor of Optimism-Human Rights between male and female, and some correlation between the attitudes and the experience of contact with disable persons or not. ConclusionIt is necessary to carry out some education of disability knowledge and rehabilitation.

The paper states the superiority of optimizing the teaching effect of clinical medicine by making use of information technology,analyzes the problems of information technology in optimizing the classroom teaching of clinical medicine,discusses the strategy of teaching with information technology in the aspect of classroom teaching structure,and thus reaches the effect of improving teaching efficiency and quality.

Objective: To construct the prokaryotic expression vector bearing fusion gene NT4-ADNF-9 and lay foundation for further study on genetic therapy of neurosensory deafness. Methods: By means of asymmetrical primer/ template, double stranded cDNA of activity dependent neurotrophic factor-9 (ADNF-9) was obtained, which included restriction enzymes sites on the two extremities. ADNF-9 cDNA was ligated to the signal and leader peptides of neurotrophin 4 (NT4), and the fusion gene was named NT4-ADNF-9. Then it was subcloned into prokaryotic expression vector pBV220, and called pBV220/ NT4-ADNF-9. Results: Evidences of DNA sequence analysis and restriction enzymes digestion showed that we recombined ADNF-9 cDNA to the 3′terminal of the signal and leader peptides of NT4, and the fusion gene was subcloned into pBV220 successfully. Bioactivity of the products was proved that it could support the cell survival and neurite growth in the primary cultures of dorsal root ganglia (DRG) of embryonic day-8 chicken neurons as compared to the control. Conclusion: Prokaryotic expression vector pBV220/NT4-ADNF-9 can be constructed successfully and the bioactivity is satisfactory.

Objective: To construct the prokaryotic expression vector bearing fusion gene NT4-ADNF-9 and lay foundation for further study on genetic therapy of neurosensory deafness. Methods: By means of asymmetrical primer/ template, double stranded cDNA of activity dependent neurotrophic factor-9 (ADNF-9) was obtained, which included restriction enzymes sites on the two extremities. ADNF-9 cDNA was ligated to the signal and leader peptides of neurotrophin 4 (NT4), and the fusion gene was named NT4-ADNF-9. Then it was subcloned into prokaryotic expression vector pBV220, and called pBV220/ NT4-ADNF-9. Results: Evidences of DNA sequence analysis and restriction enzymes digestion showed that we recombined ADNF-9 cDNA to the 3′terminal of the signal and leader peptides of NT4, and the fusion gene was subcloned into pBV220 successfully. Bioactivity of the products was proved that it could support the cell survival and neurite growth in the primary cultures of dorsal root ganglia (DRG) of embryonic day-8 chicken neurons as compared to the control. Conclusion: Prokaryotic expression vector pBV220/NT4-ADNF-9 can be constructed successfully and the bioactivity is satisfactory.

Objective To study the rehabilitation for batches of the wounded suffering from the earthquake in hospital. Methods 91 cases of trauma occurring in the earthquake were reported. Results There was no dead and any complication happened. 90 cases could self-care as they discharged. Conclusion Multiple specialty rehabilitation would be effective on the wounded suffering from earthquake.

AIM:To compare the synergistic effect of eupolyphaga on chemotherapeutics of digestive canal cancer.METHODS: Drug sensitivity of stomach cancer cell(BGC-823) was determined in vitro by MTT anti-tumor screening assay for eupolyphaga with HCPT,5-Fu,Oxa,docelaxel,by comparing cell inhibition,the synergistic effect of eupolyphaga was analysed to find the best synergistic drug.RESULTS: The synergistic effects of eupolyghaga were more significant in HCPT and Oxa than those of 5-Fu and docelaxel in vitro(P

Objective To investigate the clinical factors and therapeutic tactic for rebleeding of hypertensive hemorrhage after minimally invasive craniopuncture.Methods Clinical data of 213 cases were reviewed retrospectively.8 possible factors were gathered to select the high-risk ones by multiple-factor Logistic regression analysis.Results 27 cases bled after minimally invasive craniopuncture therapy.By Logistic regression analysis,we found 4 high-risk factors of recurrence hemorrhage,including hematoma shape (β =2.236,P =0.002),systolic blood pressure on admission (β =1.877,P =0.001),operation time (β =-1.589,P =0.004) and hematoma clearance (β =1.280,P =0.010).Conclusion Paying more attention to the 4 factors and treating each patient by individual therapeutic tactic according to the 4 factors will help to decrease the incidence of bleeding after minimally invasive craniopuncture.

Clinical data of 448 patients aged ≥60 years with osteoporotic hip fractures admitted in Shanghai Huashan Hospital between January 2010 and December 2014 were retrospectively analyzed.Among 448 cases,there were 143 males and 305 females;196 cases aged 80-89 years (43.8％) and 137 cases aged 70-79 years (30.6％);304 cases of femoral neck fracture (67.9％) and 144 cases of intertrochanteric femoral fracture (32.1％);the fractures caused by fall in 328 cases (73.2％) and comorbidity with hypertension and diabetes mellitus in 415 cases (92.6％).Two hundred and sixty eight patients were followed up for 2 years,among whom re-fractures occurred in 17 cases.The study indicates that health education is important for prevention of osteoporotic fractures in elderly people.

Objective:The purpose of this study is to investigate the feasibility of labeling skin of inner mongolia cashmere goats with BrdU,and to optimize its immunohistochemical conditions.Methods:We injected different dose of BrdU into the neck vein of cashmere goats,then we took samples from the back skin of the goats,fixed in 4% paraformaldehyde.Next the samples were embedded with paraffin and sectioned.We examined the samples with immunohistochemical technology to test the effect of the influencing factors to labeling results as the BrdU injection,such as dosage,the concentration of second antibody and streptavidin-peroxidase,the way of antigen pretreatment,and DNA denature temperature.Results:We observed red BrdU positive cells in labeled goats whereas not in the control.Meanwhile,in the samples of the unlabeled goats or those incubated with PBS,no nuclear staining is observed.Conclusion:BrdU can be used for labeling of inner mongolia cashmere goats in vivo,which have no obvious dosage effect.The best condition of labeling is that puttig the section in 2 mol/L HCl 30 min at 37℃,in trypsin 30 min at 37℃.The most appropriate concentration of biotin-conjugated second antibody and streptavidin-peroxidase is 1∶100,under which the results of immunohistochemical are the best.