Objective Bio-ceramics has been widely utilized as a common type of bone repair material.The research was designed to focus on preparation and characterization of porous TCP.Methods By using hydroxyapatite (HA) as the raw materials,and adding calcium phosphate bio-glass to adjust the ratio between calcium and phosphate,in order to achieve making porous TCP bio-ceramic scaffolds during high temperature heating process.Results Test results showed that by applying this method,TCP scaffolds had significantly higher mechanical strength then those were made from powdered TCP,and its compressive strength reached 9.11 MPa.Conclusion Tri-Calcium Phosphate (TCP) has excellent biocompatibility and it is also biodegradable.In the physiological environment,it is able to be gradually decomposed,absorbed and to promote bone growth ultimately,it is capable of replacing the damaged bone components with brand new bone tissues.

Objective:To explore therapeutic effect of rehabilitation intervention on aged patients with vascular de‐mentia (VD) .Methods :A total of 108 aged VD patients hospitalized in our hospital from Jan 2011 to Oct 2013 were selected .According to random number table ,they were randomly and equally divided into routine treatment group (received routine drug therapy and nursing ) and rehabilitation intervention group [received rehabilitation interven‐tion based on routine treatment group ,including trainings of computing ability ,thinking ability ,memory and activi‐ty of daily life (ADL) ability etc .] .ADL ability score ,mini-mental state examination (MMSE) score ,Wechsler memory scale (WMS) score were assessed ,compared and analyzed between two groups before and one year after in‐tervention .Results:There were no significant difference in ADL ,MMSE and WMS scores before intervention be‐tween two groups (P>0.05) .Compared with before intervention after one -year intervention ,there were signifi‐cant rise in ADL ability score [ (36.87 ± 9.09) scores vs .(47.54 ± 9.72) scores] ,MMSE score [ (16.87 ± 3.55) scores vs .(22.25 ± 3.41) scores] and WMS score [(59.14 ± 6.84) scores vs .(62.88 ± 6.59) scores] in rehabilitation intervention group ( P<0.05 all) ,and they were significantly higher than those of routine treatment group [ADL score :(38.31 ± 8.95) scores ,MMSR score :(16.72 ± 4.25) scores ,WMS score :(59.25 ± 6.11) scores] ,P<0.05 all .Conclusion:Rehabilitation intervention can improve cognitive function and memory and improve self -care a‐bility and quality of life in aged VD patients ,so its therapeutic effect is significant .

Extra-cellular signals, including T lymphoc ytes, cytokines,growth factors,interferons and neuropeptides, which could modulate matrix metalloproteinases expression in synoviocytes of rheumatoid arthritis(RA), are often mediated by G-protein-coupled Receptors (GPCR)-initiated signaling and mitogen-activated protein kinase (MAPK) cascades. Drug targets which aim at the extra-cellular signals or intra-cellular cascades is required for ameliorating inflammatory reaction and preventing joint destruction in RA.

Objective To study the expressions of matrix metalloproteinase-3 (MMP-3) and osteopontin (OPN) in cervical carcinoma and their relationship with invasion and metastasis. Methods The expression of MMP-3 and OPN were detected in 54 cases of cervical cancer tissues by immunohistochemical SP method. Compared with 15 cases of normal cervical tissues, the relationship was analyzed between both protein expressions and clinical pathology. Results The expression positive rates of MMP-3 and OPN in cervical cancer tissues (70.37 % and 66.7 %, respectively) were significantly higher than those of normal cervical tissues (20 % and 0, respectively), were significantly higher with infiltration depth >1/2 and lymph node metastasis than those with depth≤1/2 and without metastasis , respectively(P <0.05). The expressions of MMP-3 and OPN were related with depth of cervical infiltration and lymph node metastasis of cervical cancer.Expressions between MMP-3 and OPN were significantly positively correlation(r =0.401, P<0.05). Conclusion The expressions of MMP-3 and OPN are correlated with invasion and metastasis of cervical carcinoma.

Objective To investigate the treatment of moed high anal fistula operation.Methods 110 cases of high anal fistula patients were randomly divided into the incision line selective suture group(treatment group 55 cases) and incision group(55 cases in the control group),on two groups of treatment time,the cure rate,the anal function were analyzed.Results The cure rate of the treatment group and control group were 98 ％,86％,respectively,the cure rates of two groups have no significant difference (x2 =1.557,P ＞ 0.05),the healing time and postoperative anal function evaluation in treatment group was superior than those in control group (t =3.440,4.671,all P ＜ 0.01).Conclusion Incisionunder selective suture in the treatment of high anal fistula operation is a better way.

Objective To explore the correlations between the levels of glycosylated hemoglobin A1c (HbA1c)and N-Terminal pro-Brain Natriuretic peptide(NT-proBNP)in aged diabetes mellitus(DM)patients with heart failure(HF).Methods 127 patients diagnosed as DM complicated by HF and detected HbA1c and NT-proBNP when admitted into hospital,were divided into three groups according to the levels of HBA1C as follows:group A,4%≤HbA1c≤6.9%;group B,7%≤HbA1≤7.9%;group C,HbA1c≥8%.The levels of NT-proBNP were measured and compared.Results There was no statistical difference between group A and B(P＞0.05),where as the　levels of NT-proBNP in group C increased significantly than that in group A and B(P＜0.01);In group C,HbA1c was linear positively correlated to NT-proBNP(r=0.558,P＜0.01).Conclusion Aged DM patients complicated by HF,the level of HbA1c between 4%and 7.9%was acceptable.Meawhile,HhA1c should the independent risk prediction factor for aged DM patients complicated by HF,which could be used as the guide to the clinical therapy to improve the prognosis.

Objective To understand the clinical characteristics of lower respiratory tract infection,pathogenic distribution and drug resistance in intensive care unit to provide a basis for reasonable use of antibiotics. Methods From January 2008 to August 2010,the clinical data from our hospital ICU impatients of lower respiratory tract infection were retrospectively analyzed. The isolated strains were identified, the antibiotic susceptibility test was performed by K-B methods and the results were read according to CLSI 2007. Results There were 509 patients, 147 cases of lower respiratory tract infection were found. Lower respiratory tract infection rate was 28.9% (147/509); 283 strains of pathogens had been isolated by culture and most of which were Gram negative bacilli(86. 9%), the other pathogens included Gram positive cocci(7.4%) and fungi(5.7%). The most common pathogens were Acinetobacter spp, Pseudomonas aeruginosa, Klebsiella pneumoniae,Stenotrophomonas oligotrophic aeromons, Fungi, Escherichia coli, Staphylococcus aureus, Burkholderia cepacia,etc,accounting for 24. 7% ,20. 5% ,12. 7% ,6. 4% ,5.7% ,5. 3% ,5.3% and 4. 6% ;The main pathogens were highly resistant to the most antibiotics. Conclusion ICU had a higher lower respiratory tract infection rate, Gram negative bacilli are the main pathogen, and showed the multi-drug resistance features to the antibiotics.

Objective To set up the filter mating method of plasmid conjugation experiment for Corynebacterium.Methods The first plasmid conjugation between different genus was performed by filter method.Three strains of highly erythromycin-resistance Enterococcus faecalis isolates[minimal inhibitory concentration(MIC)of erythromycin>256 mg/L,MIC of levofloxacin≤8 mg/L] which screened by agar dilution method were used as donators and 3 strains of Corynebacteria isolates(MIC of erythromycin≤32mg/L,MIC of levofloxacin=128 mg/L)were used as acceptors.In the second conjugation between the same genus but in different species,three Corynebacterium xerosis strains(MIC of erythromycin>256 mg/L,MIC of ceftazidime=16 mg/L)which were conjugants of the first conjugation were used as donators and 6 strains of Corynebacteria isolates(MIC of erythromycin≤32 mg/L,MIC of ceftazidime 128 mg/L)were used as acceptors.Results In the first conjugation between different genus,the plasimid conjugations were done for 9 times between 3 donators and 3 acceptors,4 of which gained the phenotype and genotype of resistance and the transfer rate was 44%.In the second conjugation experiment,the plasmid conjugations were done for 18 times between 3 donators and 6 acceptors,7 of which gained the resistant phenotype and the transfer rate was 39%.Erythromycin-resistance was transferred from the donators to the conjugants in varying degrees.The resistance was transferred from Enterococcus faecalis to Corynebacteria and then to other Corynebacteria which were of the salne genus but of different species.Conclusion Filter mating conjugation method could be used to study the mechanisms of resistance transmission of Corynebacterium.

Objective: The current study investigate the immunological reaction state of Th1/Th2 cytokines expression in the peripheral blood mononuclear cells(PBMCs) from patients with lung cancer,and evaluate the effect of astragalus(AG) on it.Methods:The PBMCs from patients were divided into 4 groups:one group as pre-cultured group,and the other cells were cultured with 10%AG,20% AG and without AG (control group) ,respectively.The PBMC from 23 lung cancer patients as well as 19 healthy controls before and after the culture with AG were collected respectively. The Thl/Th2 cytokines expression were detected by RT-PCR considering IFN-r and IL-2 as Th1 cytokines and IL-4,IL-6,IL-10 as Th2 ones. Results:The results showed that the mRNA expression rate of IL-4,IL-6,IL-10 in PBMCs from lung cancer patients was obviously higher than that from the healthy controls( P

Objective To explore the curative effect of the single pore video-assisted thoracoscopic surgery and thoracic closed drainage for patients with acute pyothorax by comparing the advantage and disadvantage of two methods.Methods The clinical data of 65 patients with acute pyothorax in our hospital from January 2012 to June 2015 were retrospectively analyzed.Of which 30 patients underwent single pore video-assisted thoracoscopic surgery were as observation group,35 cases received thoracic closed drainage were as the control group.The cura-tive effect of two methods for acute pyothorax was compared by analyzing the result data of two groups.Results The postoperative chest tube indwelling time,length of hospital stay,postoperative antibiotics of observation group were significantly less than those of the control group,the difference was statistically significant(P<0.05).And the incidence of postoperative complications,the proportion of the chronic pyothorax and secondary tube rate of observation group were lower than those of the control group,the difference was statistically significant(P<0.05). Conclusion The single pore video-assisted thoracoscopic surgery is safe and effective for treatment of acute pyothorax, which is worth promoting.