The establishment animal model of Graves’ disease contributes to the study of etiology, pathogenesis and therapeutic modalities. After decades of studies and making improvements, the method of building mice model of Graves’disease has achieved a great development. Although there were many reports of animal model building in Graves’disease, as a mature technology A-subunit of thyrotropin receptor( TSHR)-expressing adenovirus was used to establish Graves’disease mice model, which has been accepted widely because of its high efficacy.

OBJECTIVE: To probe into the overall situation about the study on compatible stability of drugs. METHODS: The compatible stability of drugs reported in a total of 113 articles in 2005 in 52 kinds of periodicals were retrieved from CHKD and summarized analytically. RESULTS: The drug categories involved were chiefly antibiotics (59%), Chinese traditional medicines or Chinese drug components (20%) and other drugs (21%). Quantitative analyses in 91 articles were chiefly conducted by ultraviolet spectrophotometry (79%) or HPLC (20%). CONCLUSION: Because the ultraviolet spectrophotometry was greatly used and some experimental designs were not well-knit enough, the reliability of experimental results of compatible stability of drugs might be seriously affected, which poses a great danger to the clinical compatible use of drugs.

Objective:To observe the 5-year outcomes and complications after cataract extraction combined with intraocular lens implantation in children with bilateral congenital cataract.Methods:An observational case series study was performed.A total of 72 children (144 eyes), aged 2 to 7 years, who received intraocular lens (IOL) implantation combined with posterior continuous curvilinear capsulorhexies and anterior vitrectomy after cataract extraction for bilateral congenital cataract in The First Affiliated Hospital of Zhengzhou University from January 2007 to January 2012 were enrolled.All the patients underwent refractive correction and amblyopia training two weeks after the second operative eye underwent surgery.The patients were divided into 2-3 years old group (64 eyes), 4-5 years old group (48 eyes) and 6-7 years old group (32 eyes) according to their age at surgery.The preoperative and postoperative best corrected visual acuity (BCVA), refraction, axial length, corneal curvature, binocular visual function and postoperative complications were observed during the 5-year follow-up period.The influence of age at surgery on postoperative BCVA in one eye at the final follow-up visit was analyzed.This study adhered to the Declaration of Helsinki.The study protocol was approved by the Ethics Committee of The Affiliated First Hospital of Zhengzhou University (No.2020-KY-219), and written informed consent was obtained from guardians of the subjects before treatment.Results:The mean myopic shift was -2.10(-2.90, -1.90), -1.73(-2.50, -2.10) and-0.52(-2.00, -0.28)D in the 2-3 years old group, 4-5 years old group and 6-7 years old group, respectively, and the axial elongation in the three groups was (1.41±0.32), (0.96±0.51), and (0.52±0.26)mm, respectively, and the differences among the three groups were statistically significant ( H=19.85, P<0.01; F=13.24, P<0.05).There was no statistically significant difference in preoperative and postoperative corneal curvature among the three groups (all at P>0.05).The BCVA was significantly improved in all the eyes after operation, and a certain binocular visual function was obtained, and the visual function in the 2-3 years old group was better than that of 4-5 years old group and 6-7 years old group (all at P<0.05).The BCVA at the end of following-up was 0.3 or better in 116 eyes (80.56%), and was better than 0.8 in 28 eyes (19.44%).The regression analysis results showed that children younger at surgery had better visual outcome (postoperative BCVA=0.959-0.104 operative age of patient; R2=0.539, P<0.01). Conclusions:The recovery of visual function in children with binocular congenital cataract is related to the age at operation.The younger the age at operation is, the better the recovery of visual function will be.Timely, systematic and standardized refractive correction, amblyopia treatment and binocular visual function training after operation are helpful to the development and reconstruction of visual function.

Objective To discuss the effect of psychological intervention on prevention of posloperation infection in patients with breast cancer .Methods 336 patients with breast cancer after operation were divided into the control group and the intervention group with 168 cases in each group.The control group received routine nursing while the intervention group Was given psychological intervention based upon routine nursing.The incidence of postopemtion infection was observed in the two groups.Results Incidenc of infection took place in 27 patients(8.04%)among 336 cases.The incidence rate infection in the control group was 12.50%,which was evidently higher than that of the intervention group(3.57%),x2=9.06,P＜0.01.The infection location and time in the intervention group was better than that of the control group.Conclusion Psychological intervention could reduce the incidence rate of posteperation infection in patients with breast cancer.

Objective To investigate the application of endovascular distal parent artery occlusion in vertebral artery dissecting aneurysms involving the posterior inferior cerebellar artery.Methods The clinical and follow-up data of 5 patients with vertebral artery dissecting aneurysms involving the posterior inferior cerebellar artery who received the endovascular distal parent artery occlusion were retrospectively analyzed.Results Complete occlusion of dissected arterial and aneurysm segments was achieved in 4 patients.After followed up 6-12 months,angiography showed no recurrence or neurological deficit.Continued filling of the dissected aneurysm was observed in 1 patient's follow-up angiography,but without rehaemorrhagia or neurological deficit.Conclusions The endovascular distal parent artery occlusion is a safe and efficient choice for treating vertebral artery dissecting aneurysms involving the posterior inferior cerebellar artery,which keeps the posterior inferior cerebellar artery flowing unobstructed while clipping the dissecting aneurysm.

Objective To evaluate the advantages of MR LAVA multi-phase dynamic contrast enhancement in abdominal MRI.Methods 42 cases which clinically suspected liver lesions and 15 normal volunteers underwent MRI examination,including routine T_1WI and T_2WI scan,T_1W 2D and 3D FSPGR,LAV A multi-phase contrast enhancement.The detecting rates of 2D,3D and LAVA images were analysed and the signal noise ratio(SNR)of the liver and spleen was observed.Results 68 lesions were detected by LAVA sequence,the detecting rate was 100%,the accurate rate of diagnosis was 95.6%(65/68),while the detecting rates of the lesions were 91.2%(62/68)and 89.7%(61/68)with 3D T1W FSPGR and 2D T1W FSPGR,respectively,the accurate rates in diagnosing the lesions were 76.5%(52/68)and 70.6%(48/68)with 3D and 2D sequences,respectively.The SNR of liver,spleen of 15 normal volunteers were:29.68±2.31 in liver,36.21 ±3.67 in spleen at LAVA plain scan,46.21 ±5.32 in liver,58.75±4.68 in spleen at 2D T_1W FSPGR,19.79±2.23 in liver,23.63±2.14 in spleen at 3D T_1W FSPGR,41.75±5.13 in liver,57.96±6.13 in spleen at LAVA contrast enhancement,56.33±5.63 in liver,63.21±5.32 in spleen at 2D contrast enhancement,29.05±4.68 in liver,46.37±5.17 in spleen at 3D contrast enhancement sequence.Conclusion LAVA multi-phase dynamic contrast enhancement is more predominant than T1W 2D and 3D and 3D FSPGR in displaying the lesions and blood vessels of liver.

Objective:To investigate effects of strengthening the spleen and tonifying the kidney and their combination before and after exercise on relative indexes of metabolism of glucose and amino acids.Methods:Strengthening the spleen alone,tonifying the kidney alone or their combination before and after exercise was given to the rat receiving 7-week gradually increasing load exercise on a running platform,and after quantitative load,contents of serum lactic acid,urea nitrogen,glucose,hepatic glycogen,muscle glycogen,hemoglobin,and malondialdehyde(MDA)in renal tissue and activity of superoxide dismulase(SOD)in the renal tissue were determined.Results:Strengthening the spleen before exercise increased hepatic and muscle glycogen contents,and tonifying the kidney before exercise increased muscle glycogen content,and strengthening the spleen combined with tonifying the kidney before and after exercise increased hemoglobin level,strengthening the spleen followed by tonifying the kidney before exercise decreased serum lactic acid and urea nitrogen levels,increased muscle glycogen content and SOD activity/MDA content ratio in the kidney.Conclusion:Strengthening the spleen and tonifying the kidney play a certain role in reducing motion-related fatigue,while strengthening the spleen followed by tonifying the kidney before exercise has the best results.

Some issues of time-correlated single-photon counting technology for detection for optical properties of biological tissue are discussed,including its basic principle,components and conventional methods applying the technology.

0.05～0.2?l of HRP (Sigma Ⅵ) were introduced into the periaqueduetal gray (PAG) of 45 rats at the superior collicular level, each brain was sectioned and processed with DAB, BDHC, O-D and TMB reactions. 29 were chosen for analysis and according to the locations of the injection center and the extent of reaction, were divided into 3 groups:1. The injection center was found in the lateral part of PAG; the reaction only involved a small area within PAG (11 rats), labeled cells were seen in PAG, tectum, reticular formation (RF) and dorsal raphe nucleus.2. The injection center was in the dorsolateral part of PAG; the reaction area extended out of PAG to the nearby RF and tectum (9 rats); labeled cells were seen in the structures mentioned in group 1 as well as in habenular nucleus (hn), parafascicular nucleus (PFN), hypothalamic nuclei (anterior, posterior, ventromedial, dorsomedial, lateral), thalamic nuclei (lateral, reticular, medial part of ventral nucleus), paraventricular gray (PVG), nucleus of the optic tract, nucleus of posterior commissure (NPC), parabigeminal nucleus (PBN), locus ceruleus, ventral nucleus of lateral geniculate body, entopeduncular nucleus.3. The injection center was in the lateral or ventrolateral part of PAG, the reaction area extended to the nearby RF, tegmentum and part of tectum (9 rats), labeled cells were seen in the structures mentioned in group 1 as well as in substantia nigra, H_1, H_2, zona inserta, HN, PFN, PVG, NPC, PBN, locus ceruleus, pretectral area and nuclei of hypothalamus. In this group 3 rats were processed with TMB reaction. Labeled cells were seen not only in the above-mentioned structures but also in the deep layers of anterior cingulate cortex (areas 23 and 24), nucleus raphe magnus, nucleus reticularis gigantocellularis, periventricular gray of 4th ventricle, dentate nucleus of cerebellum, lamina Ⅴ～Ⅶ of cervical spinal cord (the other segments were left out), preoptic and supraoptic nuclei, suprachiasmatic nucleus, anterior nucleus of mammillary body, medial nucleus of amygdaloid nucleus, spinal nucleus of nerve Ⅴ. The labelled cells were seen chiefly in the ipsilateral side. Neuronal connections also were analysed according to the disparity in labelled structures.