China ; Humans ; Mouth Diseases ; Mouth Mucosa

Administration, Topical ; Humans ; Immunosuppressive Agents ; administration & dosage ; adverse effects ; therapeutic use ; Lichen Planus, Oral ; complications ; drug therapy ; pathology ; Oral Ulcer ; drug therapy ; etiology ; Tacrolimus ; administration & dosage ; adverse effects ; therapeutic use ; Treatment Outcome

Objective To understand adolescent's mental stress, coping style and their correlating with emotion. Methods This research, in order to be tried with 5870 middle school students, adopt Mental Stress Questionnaire, Coping Style Scale and Profile of Mood States. Results Compared with male students, female students had higher scores on stress from school work achievement and appearance(P＜0.05) and lower scores on stress from family[(2.04±1.19)vs(2.11±1.25)](P＜0.05),also they had higher sores on extra-coping style[(3.11±0.77)vs(2.77±0.81)](P＜0.01). Compared with lower grade students, higher grade students had higher scores on all mental stress except stress from family, while they had lower scores on extra-coping style (P＜0.01). Stepwise logistic regression analysis indicated that high scores on mental stress and intro-coping style could predict negative emotion (P＜0.01), and extra-coping style could predict positive emotion (P＜0.01).Conclusion The middle school students suffered more stress from school work achievement than from other aspects. Students who differed in sex, grade had significant different scores on mental stress and coping style. Mental stress and coping style could predict adolescents' emotion.

Objective To investigate the clinical characteristics of clavicular defect and nonunion after operation and evaluate the clinical effects of vascularized pedicled pectoralis major clavicular periosteal flap and locking plate fixation plus bone morphogenetic protein (BMP) implantation. Methods From January 2004 to April 2008, 12 patients (mean age, 42.8 years old) with clavicular defect and nonunion were treated in Zhongshan Hospital. The internal fixators were removed, and then fibrous scars and sclerotic bones were cleared. The clavicular detects were fixed with locking plate, with implantation of the BMP. The wound was covered with vascularized pedicled pectoralis major clavicular periosteal flap. Postoperative functional rehabilitation exercises were performed. Results All patients were followed up for 8-24 months (mean 1.2 years). The clinical results were evaluated by Constant-Murley scoring system. The clavicular defects were healed in all patients within 4-7 months, and the internal fixators were successfully removed from some of the patients. Conclusions Vascularized pedicled pectoralis major clavicular periosteal flap combined with locking plate fixation plus BMP implantation is effective in the treatment of clavicular defect and nonunion.

Quantitative measurement of strain distribution of arterial vessel walls due to pulsatile blood flow within the vascular lumen is valuable for evaluating the elasticity of arterial wall and predicting the evolution of plaques. The present paper shows that the three-dimensional (3D) strain distribution are estimated through uni-directional coupling for 3D vessel and blood models reconstructed from intravascular ultrasound (IVUS) images with the computational. fluid dynamics (CFD) numerical simulation technique. The morphology of vessel wall and plaques as well as strain distribution can be visually displayed with pseudo-color coding.
Arteries ; diagnostic imaging ; physiology ; Elasticity ; Hemodynamics ; Humans ; Imaging, Three-Dimensional ; Pulsatile Flow ; Ultrasonography

Objective To analyze the distribution rule of TCM syndromes and syndrome elements in chronic atrophic gastritis with osteoporosis by retrospective study method, and provide evidence for TCM treatment. Methods TCM symptoms, syndromes and syndrome elements of 127 cases of chronic atrophic gastritis with osteoporosis were retrospectively investigated and statistically analyzed. Results The distribution of TCM syndromes in chronic atrophic gastritis with osteoporosis was as follows:there were 51 cases of asthenia syndrome of both qi and yin, accounted for 40.16%;28 cases of yang deficiency syndrome of spleen and kidney, accounted for 22.05%;21 cases of syndrome of qi deficiency and blood stasis, accounted for 16.54%. The distribution of syndrome elements was:there were 82 cases of qi deficiency, accounted for 64.57%;61 cases of yin deficiency, accounted for 48.03%;28 cases of yang deficiency, accounted for 22.05%;21 cases of blood stasis, accounted for 16.54%;12 cases of qi stagnation, accounted for 9.54%;6 cases of dampness-heat, accounted for 4.72%. Conclusion The basic pathogenesis of chronic atrophic gastritis with osteoporosis is deficiency of both spleen and kidney. Deficiency of kidney is mainly qi deficiency, yin deficiency, yang deficiency, or even deficiency of both yin and yang. Reinforcing spleen and kidney is the important principle of treatment.

Laparoscopic partial nephrectomy is gaining more and more popularity in treating patients with early renal carcinoma in recent years.Compared with radical nephrectomy,laparoscopic partial nephrectomy is a mini-invasive,safe method with satisfactory postoperative survival rate.However, there were still limited laboratory and clinical data about laparoscopic partial nephrectomy,and the experimental data of traditional laparoscopic surgery and open surgery were still used.Based on the existing data and the authors' experience,this article proposes three hypotheses for the problems puzzling urologic surgeons;the authors hope to verify the hypotheses through basic or clinical research.

OBJECTIVE:To observe the efficacy and safety of Zhenqi fuzheng granule combined with dasatinib in the treat-ment of BCR/ABL positive leukemia. METHODS:40 patients with BCR-ABL positive chronic myeloid leukemia or PH-positive acute lymphoblastic leukemia were randomly divided into control group(20 cases)and observation group(20 cases). Control group received Dasatinib tablet,CML patients in chronic period received 100 mg,once a day,orally,morning or evening;CML patients in acceleration period and blastic period received 140 mg,once a day,orally,morning or evening,swallow whole,no cut or crushed. Observation group additionally received 15 g of Zhenqi fuzheng granule,twice a day. The treatment course for both groups was 24 months. Hematologic response,cytogenetic response and molecular biological response in 2 groups were observed, fatality rate,mean follow-up time and the incidence of adverse reactions were followed-up. RESULTS:CCR in observation group was significantly higher than control group,mean follow-up time was longer than control group,CCR median time was significant-ly shorter than control group,the incidence of adverse reactions and fatality rate were significantly lower than control group,the differences were statistically significant(P<0.05). There were no significant differences in the cytogenetic response and molecular biological response in 2 groups(P>0.05). CONCLUSIONS:Zhenqi fuzheng granule combined with dasatinib is superior to dasat-inib alone in proving hematologic response and safety in the treatment of BCR-ABL positive leukemia,and similar in cytogenetic response and molecular biological response.

Objective To observe the expression of phosphorylation of signal transducer and activa -tor of transcription 5 (STAT5) protein and the DNA-binding activity of STAT5 after interleukin 2 therapy for genital herpes .Methods Six patients with recurrent genital herpes were included in this study .CD4 +T cells from patients who underwent interleukin 2 therapy were analyzed for changes in STAT 5 signaling.None had been treated with corticosteroid and immunosuppressive agents .Phosphorylation of STAT 5 was detected in the T cells using Western blot analysis .Electrophoretic mobility shift assay ( EMSA) was carried out to detect the DNA-biding activity of STAT5.Results The expressions of phosphorylated STAT 5 in T cells de-rived from patients with genital herpes 28 days after interleukin treatment were 1.8~2.7 fold to that of be-fore the treatment was given .STAT5 DNA-binding activity in T cells derived form patients with genital her-pes who had received the treatment was 2.3~3.4 fold increased compared to that of before the initiating of interleukin 2 treatment.The differences between the before and after interleukin 2 treatment were statistical-ly significant( t =10.6, P <0.05).Conclusion This study indicates that STAT5 may be an important signaling mediator of interleukin 2 therapies , and that STAT5 activation may predict response to this treat-ment .

A new closed bipolar electrode electrochemiluminescence ( ECL)-based device was designed, and further used to investigate the ECL behaviors of luminol in this device. Our results showed that, while a suitable voltage was applied to the two poles of the closed bipolar electrode, both the positively charged ions and luminol-based anionic ions could be enriched on the two poles of the closed bipolar electrode, respectively. More importantly, the ECL signals, generated from the electro-oxidation of luminol on anodic pole, were found to be related to the total amount of positively charged ions on the cathodic pole of the closed bipolar electrode. Under the optimum experimental conditions, the ECL response was linearly to the concentration of analyte in the range of 1. 0×10-9-1. 0×10-8 mol/L with a detecting limit of 1. 1×10-10 mol/L. Based on this finding, a new ECL method for sensing the solution conductance was developed.