During L-lactic acid fermentation with Rhizopus oryzae,there existed a branch pathway by which pyruvate was transformed to eth- anol catalyzed by pyruvate decarboxylase(PDC)and alcohol dehydrogenase(ADH),thus decreasing the flux of pyruvate to lactic acid.In this study,the spores of Rhizopus oryzae AS3.3462 mutagenized with nitrosoguanidine(NTG),the appropriate dosage was 0.15 mg/mL and the lethal rate was 70%～80%.Two mutants,named mut-1 and mut-2,with decreased ADH activity were screened out by yeast peptone dextrose(YPD)agar medium containing allyl alcohol.These two mutants had decreased ADH activities of 41.63% and 50.29% compared with the parent strain.The fermentation behavior after 72h showed that the yields of ethanol produced by mut-1 and mut-2 were 4.87g/L and 6.56g/L respectively,while the wild type strain was 28.9g/L,and the lactate concentrations of mut-1 and mut-2 also increased from 40.31g/L to 54.45g/L and 44.07 g/L,respectively.It is also found that mut-1 and mut-2 had a high reducing sugar consumption rate and biomass accumulation than its present strain

Objective To evaluate the cool-tip radiofrequency treatment of uterine myoma. Methods 42 volun-tary patients were treated with cool-tip radiofrequeney electrode,in ultrasound-guided,via abdominal or vaginal inser-tion the uterine myoma. The diameter of 56 uterine myomas was from 3.0 cm to 8.0 cm. Results After the treat-merit,following-up the 56 uterine myomas of 42 patients for more than 3 months,the curative rate was 100%. Con-clusion With tiny wound and remains the functions of uterus, the cool-tip radiofrequency is an effective, simple,safe and reliable technique on the therapy of uterine myoma. It can be applied to any site of the uterine myoma.

ObjectiveTo investigate the effect of dexmedetomidine on the median effectivetarget effectsite concentration (EC50) of remifentanil required for preventing body movement in response to skin incision made under propofol sedation.MethodsForty ASA Ⅰ or Ⅱ patients aged 20-50 yr weighing 45-58 kg scheduled for elective breast tumor excision were randomly allocated into 2 groups ( n =20 each):group remifentanil (group R) and group remifentanil + demedetomidine ( group RD).Sedation was induced with propofol TCI at target plasma concentration of 3.0 mg/L in both groups.In group RD dexmedetomidine 1.0 μg/kg was infused iv over 10 min before start of propofol TCI,while in group R equal volume of normal saline was infused instead of dexmedetomidine.Remifentanil TCI was started with target effect-site concentration set at 3.0 and 2.5 μg/L in groups R and RD respective at 13 min after beginning of propofol TCI.Skin incision (3 cm in length) was made when the target concentrations of propofol and remifentanil TCI were reached.Body movement was assessed by a nurse not involved in this study.EC50 and 95％ confidence interval (CI) of remifentanil were determined by up-and-down technique.The target effect-site concentration was increased or decreased by 20％ depending on the response of the previous patient to skin-incision.ResultsThe EC50 of remifentanil for preventing body movement in response to skin incision performed under propofol sedation was 1.7 μg/L (95％ CI 1.5-1.9 μg/L) and 2.5 μg/L (95％ CI 2.2-2.7μg/L) in groups RD and R respectively.The EC50 of remifentanil was significantly lower in group RD than in group R.ConclusionDexmedetomidine 1.0 μg/kg can decrease EC50 of remifentanil for preventing body movement in response to skin incision made under propofol sedation.

Objective To investigate the effect of dexmedetomidine on bispectral index (BIS) value at loss of consciousness (LOC) caused by propofol given by target-controlled infusion (TCI).Methods One hundred and twenty ASA Ⅰ or Ⅱ patients,aged 20-50 yr,weighing 41-68 kg,scheduled for general surgery were randomly divided into 3 groups (n =40 each):propofol group (group P),dexmedetomidine 0.5 μg/kg + propofol group (group D1P) and dexmedetomidine 1.0 μg/kg + propofol group (group D2P).The patients in each group were randomly assigned into 5 subgroups ( n =8 each):groups P0-4 receiving TCI of propofol with the target effect-site concentration (Ce) set at0,1,2,3 and 4 mg/L respectively.Groups D1P0-4 received iv infusion of dexmedetomidine 0.5 μg/kg at a rate of0.05μg·kg-1 ·min-1 and TCI of propofol with the target Ce set at 0,1,2,3 and 4 mg/L respectively at 5 min after the end of dexmedetomidine infusion.Groups D2 P0-4 received iv infusion of dexmedetomidine 1.0 μg/kg at a rate of 0.1μg· kg- 1· min- 1 and TCI of propofol with the target Ce set at 0,1,2,3 and 4 mg/L respectively at 5 min after the end of dexmedetomidine infusion.Three minutes after TCI of propofol was started,OAA/S score and BIS value were recorded.The OAMS score ≤ 2 was defined as LOC.The EC50 and 95％ confidence interval of propofol for LOC and BIS50 and 95％ confidence interval at LOC were calculated by Probit analysis.Prediction probability (Pk) of BIS value at LOC was calculated using Smith method.Results Compared with group P,EC50 was significantly decreased,BIS50 was significantly increased ( P ＜ 0.05 or 0.01 ),and no significant change was found in Pk in groups D2 P and D1 P ( P ＞ 0.05).There were no significant differences in EC50,BIS50 and Pk between groups D2 P and D1P ( P ＞ 0.05).Conclusion BIS value can accurately predict the level of consciousness during anesthesia with dexmedetomidine and TCI of propofol,but BIS value is increased at LOC.

Objective To investigate the hemodynamic changes of the proper hepatic artery (PHA) in hepatocellular carcinoma (HCC) and portal stem vein tumor thrombus.Methods Sixty HCC and portal stem vein tumor thrombus patients were divided into partial occlusion group and completely occlusion group with 30 patients each.Eighty HCC patients without portal stem vein tumor thrumbus were in HCC group and 20 health cases were in control group.Color Doppler ultrasound was used to measure the diameter of PHA (PHA-D),systolic maximal velocity (Vmax) and resistant index (RI) of PHA.Results PHA-D in control group,HCC group,partial occlusion group and completely occlusion group was (0.33 ±0.05),(0.44 ±0.04),(0.45±0.04),(0.61±0.07)cm,Vmax was (31.32±9.31),(66.76±20.34),(68.16±21.96),( 132.65 ± 38.84 ) cm/s,RI was 0.75 ± 0.08,0.68 ± 0.13,0.65 ± 0.11,0.55 ± 0.10,respectively.The levels of PHA-D,Vmax and RI in HCC group,partial occlusion group and completely occlusion group had significant difference compared with those in control group (P ＜ 0.01 or ＜ 0.05 ).The levels of PHA-D,Vmax and RI in completely occlusion group had significant difference compared with those in HCC group,partial occlusion group(P＜ 0.01 ). Conclusions The blood flux obviously ascends compensatorily in HCC with portal stem vein tumor thrombus,RI obviously descends.But it is the occlusive nature of the tumor thrombus that is crucial to the hemodynamic change of the proper hepatic artery.

Objective: To explore the effect of psychotherapy in treating middle school students with IAD (internet addiction disorder). Methods: 23 cases of middle school students with IAD had been treated by cognitive therapy or behavior therapy. The IAD scale, SCL-90 scale and SDS scale were used to evaluate the effect before and after therapy. Results: After therapy, the total scores and most dimensions scores in IAD scale, SCL-90 scale and SDS scale decreased significantly than that before therapy. Conclusions: Psychotherapy especially cognitive therapy and behavior therapy is an effective in treating middle school students with IAD.

It has been shown that the PIK3CA mutation in HER2 positive breast cancer is up to 25%, and thus activates PI3K-Akt signaling pathway,promotes HER2 mediated tumor cell epithelial transformation, alters the intrinsic phenotype of HER2 overexpression breast cancer,and finally leads to resistance to anti HER2 targeted therapy.Some studies have shown that the PIK3CA gene mutation is associated with the efficacy of anti-HER2 targeted therapy.Therefore,real-time monitoring of PIK3CA gene mutation will promote indivi-dualized anti-HER2 targeted therapy.

Objective: To study the effect of the ryegrass powder on the insulin resistance in obese rats. Method: Thirty four Wistar rats were divided into three groups by random:control group fed with normal diet, both obese group and ryegrass powder group fed with high lipid diet for 6 w and then the ryegrass powder group fed ryegrass powder of 0 and 2 g/(kg bw?d) respectively for 4 w. Then blood lipids, glucose,free fatty acid and insulin were measured. Euglycemic clamp technique was used. Results: The blood glucose, insulin, TG, TCH, LDL and FFA of the obese group were obviously (P

35 patients with malignant lymphoma and 18 patients with multiple mycloma were investigated for expression of OKT10in pherpheral blood. A significant incresing of OKT 10 positive cells was found in most of patients (26/30, 16/18 cases respectively) with Non-Hodgekin's Lymphoma(NIIL) and Multiple myeloma(MM). The inereasing of OKT10 positive cells is related to the degrce of malignaency of patients with NIIL from low-grade to high-wade. No relationship between serum beta-2-microglobulin and OKT10 positive cells in patients with NIIL and MM was also found.

0.05).Conclusions Special protein gold measuring instrument had high sensitivity,good accuration and speediness,which was suitable for clinical LAB to test CRP.