Objective:To completely solve the traditional ECG examination mode, establish ECG diagnosis center in hospital, realize the automation of the whole process of ECG.Methods: Demonstrate the structure and function of Network ECG information management system software and hardware, describes the key technology used in the process of implementation.Results: Lead to centralized storage, management and diagnosis of the ECG data, achieve data sharing through integration with HIS, PACS system.Conclusion: Optimized the ECG examination process, improve doctor's efficiency and ECG report quality, make it convenient for the doctor to consult and analyze ECG data, provide medical care and powerful technical support for teaching and research.

Hospital digitization is a systematic process and has to be accomplished by stages. This paper discusses the key points of all stages of hospital digitization to enhance the control level of hospital.

Objective To establish the method for determining ephedrine hydochloride and pseudoephedrine hydrochloride in Keke Tablets by HPLC. Methods The samples were analyzed by a phemomenex Synergi Polar-RP column (4.6 mm×250 mm, 4 μm), with mobile phase methanol∶0.092%phosphoric acid (0.04%triethylamine and 0.02%second butylamine)=1.5∶98.5 at flow rate of 1.0 mL/min and detection at UV wavelength of 210 nm. The column temperature was 30 ℃. Results The linear ranges of ephedrine hydrochloride and pseudoephedrine hydrochloride were 6.51×10-3-0.651 μg (r=0.999 9) and 6.27×10-3-0.627 μg (r=1), respectively. The average recoveries (n=6) of ephedrine hydrochloride and pseudoephedrine hydrochloride were 102.26%and 103.71%, with RSD of 0.34%and 0.22%, respectively. Conclusion This method is simple, accurate, reproducible, highly specific and reliable results, and has the ability to effectively control the quality of alkaloids in Keke Tablets.

The study of tumors' proteomics is a research hotspot in China and abroad in recent years.The development of theory and technology in proteomics has provided new ideas and research field for cancer research.This paper reviewed the commonly used technical means of proteomics and its research progress on diagnosis,therapy and other aspects of pancreatic carcinoma.

At present,in the opinions of the domestic and foreign scholars,the only hope of long-term survival even cure of hilar cholangiocarcinoma was obtained by radical resection(ie R0 resection).The application of preoperative assessment with appropriate and comprehensive strategies can judge the respectability and Intraoperative safety accurately to the tumor,thereby it can improve the radical resection rate and safety of patients with hilar cholangiocarcinoma.In this paper,preoperative assessment strategies are reviewed.

Objective To systematically review the risks and effectiveness of combined caudate lobectomy in Klatskin tumor.Methods A systematic literature search was conducted on PubMed,Embase,Cochrane Library,Scopus,CNKI databases and Chinese Bio Medical Literature (CBM).A systematic review and meta-analysis of the current literatures were conducted based on the PRISMA guideline.Odds ratios were calculated using the Mantel-Haenszel method.The primary outcome variables included safety,effectiveness of combined caudate lobectomy and its impact on long-term survival.Results Nine out of 312 articles were included.Results showed caudate lobectomy did not increase the incidence of postoperative complications (OR ＝ 1.15；95 ％ CI 0.74-1.81；P ＞ 0.05),but improved R0 resection rate (OR ＝ 4.81；95 ％ CI 2.72 ～ 8.53；P ＜ 0.01) and long-term survival in these patients (OR ＝ 0.20；95％ CI 0.11 ～ 0.39；P ＜0.01).Conclusion Combined caudate lobe resection is a safe and effective treatment for Klatskin tumor.

Objective To explore the role of zinc finger protein (ZNF)185 in the proliferation of human glioma cells. Methods Human glioma tissues and tumor adjacent tissues were obtained from glioma patients diagnosed pathologically in Tangshan Gongren Hospital from January 2011 to December 2013. Total protein was extracted from different tissues. The ZNF185 expression was detected by Western-blot assay. Total RNA was extracted from tumor adjacent tissues. ZNF 185 cod?ing sequence was obtained by RT-PCR and inserted into pEGFPC2 plasmid to construct the ZNF185 expression vector. Li?pofactamine2000 was used to transfect the ZNF185 expression vector to human glioma cell SF767. pEGFPC2 blank vector transfected SF767 cells were used as control. Changes of cell cycle were analyzed by flow cytometry, and cell proliferation was analyzed by MTT assay. Results The expression of ZNF185 decreased significantly in human glioma tissues compared to that of tumor adjacent tissues(P<0.01). The over expression of ZNF185 in SF767 resulted in the increased proportion of cell cycle G0-G1phase, but decreased proportion of S phase(P<0.05). Furthermore, the cell proliferation was significantly inhibited in the ZNF185 over-expressed SF767 cells compared with that of blank vector transfected cells(P<0.05). Con?clusion ZNF185 plays an inhibitory role in cell proliferation of human brain glioma cells.

OBJECTIVE:To provide reference for the timely and accurate judgment of drug fever induced by Piperacillin sodi-um and tazobactam sodium for injection and rational drug use in the clinic. METHODS:The medication,clinical manifestations, laboratory examination results and the physician treatment of 30 inpatients with drug fever induced by Piperacillin sodium and tazo-bactam sodium for injection from Sept. 2013 to Sept. 2014 were analyzed retrospectively and statistically. RESULTS:Most of the drug fever induced by Piperacillin sodium and tazobactam sodium for injection occurred in continuous 7 to 14 d medication,and the cumulative dosages were between 1.3 to 2.7 g/kg;73.3% of the 30 patients had fever during the intravenous drip,with body temperature mainly≥38.5 ℃;the elevation of eosinophil and slightly increase of serum C-reactive protein (CRP) and blood cell sedimentation rate (ESR) could be used as observation indexes of drug fever,but the leucocyte reduce could’t be suitable;the body temperature dropped to normal within 24 to 48 hours after stopped using it. CONCLUSIONS:The drug fever induced by Piperacillin sodium and tazobactam sodium for injection has no correlation with the patients’gender or age,and no special diagnos-tic criteria. But it has certain correlation with duration of medication,cumulative days and dosages and it can be used as reference of judgment with the combination of hematological examination index. Clinicians should improve the understanding and attention about drug fever to stop using suspicious drug in time.

Epidemiological studies have shown that patients with diabetes are prone to have depression and anxiety.This article reviews the evidence,mechanism,complications and strategy of selective serotonin reuptake inhibitors paroxetine in treatment of depression and anxiety in patients with type 2 diabetes mellitus.

Purpose:During the malignant transformation, most tuomr cells will become resistant to anoikis (the apoptosis induction upon loss of anchorage), thereby gaining an important growth advantage and providing the basis for tumor spread, growth at distant places, and formation of metastasis.The present study focus on the role of soluble fibronectin, integrin and their signal transduction in the process of tumor cells anoikis apoptosis.Methods:Flow cytometry detected integrin subunits' expression on the human squamous cell carcinoma cell (HSC 3) surface. Establish anoikis induction model. The role of different integrin subunits during anoikis induction were detected by antibody blocking method, p53 and Bcl 2/Bax expression were measured during this anoikis process.Results:There is no or low expression of integrin av on the normal epithelial cells, whilst it expresses relative higher on the surface of HSC 3 cells. The treatment of soluble fibronectin decreased p53 expression, increased the ratio of Bcl 2/Bax and then protected HSC 3 cell from anoikis. Anti integrin ?v blocking antibody resumed the anoikis that was inhibited by soluble fibronectin evidently. Conclusions:We now provide the evidence that the binding of integrin ?v and soluble fibronectin protected HSC 3 cells from anoikis apoptosis, via decreasing the expression of p53, increase the ratio of Bcl 2/Bax.