The animal experiments,clinical researches and epidemiological investigations indicated that genistein has the effect for prevention and treatment of osteoporosis,cardiovascular disease and cancer.Genistein can inhibit cellular and humoral immunity and can regulate cell apoptosis.The chemical structure,biological effects and the mechanism of genistein attracted much attention.The current research advance on genistein was summarized and the prospect on the theoretical significance,clinical importance was presented in this paper.

Hypothyroidism is one of the most common endocrine disorders in childbearing or pregnant women.Gestational hypothyroidism can increase the incidence rate of the variety of pregnancy complications and adverse pregnancy outcome.It also can make adverse effects on the fetus and neonate,such as death,placental abruption,fetal malformation,intrauterine growth retardation and preterm,low birth weight,neonatal respiratory distress syndrome,it can also cause abnormal thyroid function of neonate,severely can cause nerve,mental,physical and mental developmental abnormalities and even death.Therefore,it is recommended that childbearing women who are at high risk for pregnancy should strengthen to monitor pregnancy thyroid function and treat as soon as possible in order to reduce the adverse effects on offspring.

OBJECTIVE: To evaluate the current situation and trend of narcotic drug use in our hospital where the authors work in order to provide the reference for scientific management and rational use of these drugs. METHODS: The yearly amount of narcotic drugs administered in the whole hospital, the yearly amount in the separate departments, as well as drug expenditures and ratios between January 2002 and November 2005 were added up and analyzed statistically. RESULTS: The amount of bucinnazine use dominated in the first. The amount of morphine for oral use was increasing year by year. The amount of fentangl transdernal patch use was also bigger and increasing rapidly. However, the amount of pethidine and morphine for injection use was decreasing. CONCLUSION:Analgetics for oral and transdermal use will be the main categories of analgetics in the future.

Objective To investigate the effect of mycophenolic acid (MPA) on the proliferation of rat pulmonary microvascular endothelial cells (PMVECs) and the effects of MPA on the secretion of endothelin-1 (ET-1) and interleukin-6 (IL-6).Methods PMVECs were cultured by tissue-sticking method.Cells were divided into 5 groups,named as the control group,low-dose MPA group,moderate-dose MPA group,highdose MPA group and super high-dose MPA group,according to the final concentration of MPA (0,0.1,1,10 and 100 μmol/L,respectively).The proliferation of rat PMVECs was detected by cell counting kit-8 (CCK-8).The ET-1 and IL-6 secretion were assessed by radio-immunoassay.One-way analysis of variance was used to detect the differences between the group means.Results The cultured PMVECs of rats in vitro showed fusiform or polygonal in shape,and the expressed factor Ⅷ associated antigen stain was positive.At 24 h,the A values of the 5 groups (from control group to super high-dose MPA group) were 1.15±0.20,1.20±0.13,0.88±0.20,0.62±0.05 and 0.56±0.10,respectively,there was no significant difference in the A values on 450 nm between the control group and the low-dose MPA group (P=0.388),as well as the high-dose MPA group and super high-dose MPA group (P=0.292),but there were significant differences between any other two groups (P＜0.01).At 48 h,the A values of the 5 groups (from control group to super high-dose MPA group)were 2.61±0.21,2.52±0.23,1.99±0.17,0.84±0.16 and 0.65±0.12,respectively,there was no significant difference in the A values on 450 nm between the control group and low-dose MPA group (P=0.094),but there were significant differences in the A values between any other two groups (P＜0.01).At 72 h,the A values of the 5 groups (from control group to super high-dose MPA group) were 2.62±0.24,2.62±0.19,2.37± 0.38,0.79±0.17 and 0.61±0.17,respectively,there was no significant difference in the A values on 450 nm between the control group and low-dose MPA group (P=0.931),but there were significant differences in the A values between any other two groups (P＜0.05).There were no significant differences in the ET-1 concentrations between the control group and low-dose MPA group (P=0.156),and high-dose MPA group and super high-dose MPA group (P=0.262),but there were significant differences in the concentrations between any other two groups (P＜0.01).There were no significant differences in the IL-6 concentrations of the five groups between any of the two groups (P＞0.05).Conclusion MPA can inhibit fetal bovine serum induced rat PMVECs proliferation and the secretion of ET-1 in a dose-dependent manner.

The development of molecular biology techniques makes important contributions to the researches of heritable varia-tion of Schistosoma. In recent years,the molecular genetic analysis in the Schistosoma variation researches mainly includes the re-striction fragment length polymorphism(RFLP),random amplified polymorphism technology(RAPD),microsatellite anchored PCR(SSR-PCR),and polymerase reaction single-strand conformation polymorphism(PCR-SSCP). This article reviews the re-search progress of molecular genetic analysis in Schistosoma variation in recent years.

Objective To explore the therapeutic mechanism of Xiao Chaihu Decoction for endometriosis in rats. Methods The rat models of endometriosis were treated with Xiao Chaihu Decoction, Danazol, and Xiao Chaihu Decoction plus Danazol for 4 weeks,respectively. And then cyclooxygenase-2(COX-2) expression in endometrium and endometriotic tissue was observed by immunohistochemistry method. Results The expression of COX-2 in the endometriotic tissue was lower than that in endometrium in Xiao Chaihu Decoction group and Xiao Chaihu Decoction plus Danazol group, while the results in Danazol group and control group were opposed to those in the above two groups. Conclusion The mechanism of Xiao Chaihu Decoction for rats endometriosis may be related to the decrease of COX-2 expression in ectopic Endometrium, thus reducing the formation of prostaglandins.

OBJECTIVE:To discuss the effect of recombinant human growth hormone(HGH)on serum sndotoxin,inflamma-tory cytokines,wound healing time,hospitalization time and prognosis of elderly patients with severe burn. METHODS:96 elder-ly patients with severe burn in our hospital were randomly divided into treatment group (48 cases) and control group (48 cases). All patients received wound surface care,nutritional support,maintaining water and electrolyte and acid-base balance. Control group received 1 ml 0.9% Sodium chloride solution,sc,qd. Treatment group received 0.3 IU/kg. Recombinant HGH for injection, sc,qd. All patients were treated for 21 d. Serum endotoxin content in 2 groups before and 14,21 d after treatment was compared, inflammatory cytokines (TNF-α,IL-6, IL-8) before and after treatment,wound healing time,hospitalization time and the inci-dence of multiple organ disorder syndrome(MODS),death and systemic infection were observed. RESULTS:There was no signifi-cant difference in serum endotoxin content before and 14 d after treatment(P>0.05);after 21 d,serum endotoxin contents in 2 group obviously decreased,and treatment group was significantly lower than control group,with statistical significance(P<0.05). After treatment,IL-8 level in treatment group was significantly higher than control group,TNF-α and IL-6 were significantly lower than control group,with statistical significances (P<0.05). Wound healing time and hospitalization time in treatment group were significantly shorten than control group,with statistical significances(P<0.05). The incidence of MODS,death and systemic infec-tion in treatment group were significantly lower than control group,with statistical significances(P<0.05). CONCLUSIONS:The application of recombinant HGH in elderly patients with severe burn can reduce toxins absorption of patients and decrease inflamma-tory cytokines to promote wound healing and improve prognosis.

OBJECTIVE:To observe the clinical efficacy and safety of Shuxuetong combined with acupuncture in the treat-ment of patients with stroke during recovery period. METHODS:80 patients with stroke during recovery period were selected and divided into observation group and control group,with 40 cases in each group according to random number table method. Control group received acupuncture,6 times a week. Observation group was additionally given Shuxuetong injection 6 ml added into 0.9% Sodium chloride injection 250 ml,ivgtt,qd,for one month. Clinical efficacy of 2 groups were observed as well as in-tracranial [arteriae cerebri anterior (ACA),middle cerebral artery (MCA),basilar artery (BA)] blood flow rate,neurological deficit score(NIHSS),cognitive ability(MMSE)score,Fugl-Meyer activity score and Fugl-Meyer balance score before and af-ter treatment. The occurrence of ADR was compared. RESULTS:Total effective rate of observation group was 95.0%,which was significantly higher than that of control group(70.0%),with statistical significance(P<0.05). Compared with before treatment, blood flow rate of ACA and MCA were increased significantly in observation group after treatment,and were higher than those of control group with statistical significance (P<0.05). NIHSS,MMSE,Fugl-Meyer activity score and Fugl-Meyer balance score of 2 groups improved significantly after treatment,the observation group was better than the control group,with statistical signifi-cance(P<0.05). No obvious ADR was found in 2 groups. CONCLUSIONS:Shuxuetong combined with acupuncture show signif-icant therapeutic efficacy,and improve intracranial blood flow rate and neurologic function with good safety.

OBJECTIVE:To observe the effect of the side-hole hollow bone cement nail on promoting bone fracture healing. METHODS:This experiment was performed in the Third Hospital of Hebei North College from July 2006 to December 2008. Femoral neck samples were obtained from 60 adult corpses of which the patients were dead one year at most with the mean age of 70 years. X-ray examination showed a significant osteoporosis,but no other abnormalities. Hollow nail was provided by Sanyou Medical Apparatus Co.,Ltd.,Shanghai; self-cohesion methyl methacrylate bone cement was provided by Dental Material Factory,the Second Medical University of Shanghai. In the maximal axial pulling-out force group,the hollow nails were directly implanted into 10 femoral neck samples after drilling at the axial center. The polymethyl methacrylate bone cement was inserted into other 10 femoral neck samples after drilling at the axial center,and the side-hole hollow nail were then implanted. The pulling out experiment was performed by AGS-10KN material testing machine to measure the maximal axial pulling-out strength of hollow nail and the maximal loading when the nail was loose during anti-bending cycle experiments. RESULTS:① The maximal axial pulling-out force of 10 femoral neck samples implanting the hollow nail was 471.5-1 070.5 N,with the mean value of (855.0 ? 207.5) N; the maximal axial pulling-out strength of femoral neck samples implanting the side-hole hollow nail added by poly-methyl methacrylate bone cement was 2 023.7-3 572.1 N,with the mean value of (2 943.3 ? 535.8) N. The maximal axial pulling-out strength in the combined group was significantly greater than hollow nail implantation group (P

Bone marrow stromal cells(BMSCs) is a kind of adult stem cells which exists widely in human mesenchymal tissues and have active abilities of self-replication and multi-differentiation. When intervened individually or jointly by certain inducing agents and by the kind of genes, it can differentiate into specific lineage of cells in vitro and the capacity of multilineage differentiation and proliferation does not change. At the same time, with the unique immunological characteristics,it is an ideal seed cell for tissue engineering. In recent years, a lot of studies have proved that BMSCs can not only be induced and differentiate into nerve cells under certain conditions but have a certain structure and function of the nerve cells, which provide a new way in the treatment of nerve system diseases.This paper gives a review of studies on induced differentiation of BMSCs into nerve cells and the functions.