1.Clinical characteristics and molecular genetics of Dent's disease: an update.
Chinese Journal of Pediatrics 2012;50(12):909-912
Animals
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Child
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Child, Preschool
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Chloride Channels
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genetics
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Dent Disease
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complications
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diagnosis
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genetics
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therapy
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Diuretics
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therapeutic use
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Humans
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Hydrochlorothiazide
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therapeutic use
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Hypercalciuria
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diagnosis
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genetics
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Mutation
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genetics
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Phosphoric Monoester Hydrolases
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genetics
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Proteinuria
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diagnosis
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etiology
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genetics
5.Evaluation of radiation dose in multislice spiral CT scan for children embedded teeth
Hongbo XIA ; Xiaohong WANG ; Lihai SONG ; Changying ZHENG ; Guangyu XU ; Kun XIANG
Chinese Journal of Radiological Medicine and Protection 2010;30(1):75-76,78
Objective To evaluate the radiation dose in both low radiation dose and to conventional radiation dose mode in multislice spiral CT scan for children with embedded teeth,and explore the lowest conditions of exposure in multislice spiral CT scan for children embedded teeth.Methods Eighty cases of children with embedded teeth examined by 16 slices spiral CT were divided into 5 ,25,125 and 250 mAs scanning groups.The scanned pictures of four groups were evaluated by two chief physicians and three associate chief physicians in the radiological department with double blind method.The CTDI_w and DLP in different radiation dose mode were recorded and analyzed with statistics.Results All the four groups scanned pictures in different radiation dose mode could accurately show the structure and the adjacency relation of embedded teeth.Even though the resolution of scanned pictures in S mAs group were inferior to the other three groups on bone trabecula,gingiva and their surrounding soft tissue,it still could meet the requirements of examination.The average DLP in 5 mAs group was only 2% of value in 250 mAs group.Conclusions MSCT in 5 mAs group can not only meet the requirements of examination,but also reduce the radiation dose to patients to the greatest extent.
6.Normal Representations of Permanent Teeth and Alveolar Bone at Multi-slice Spiral CT
Hongbo XIA ; Yunxia LI ; Shucheng ZHANG ; Xiaohong WANG ; Guangyu XU ; Changying ZHENG ; Kun XIANG
Journal of Practical Radiology 2001;0(05):-
Objective To explore the spiral the normal representations of permanent teeth and alveolar bone of multi-slice spiral(MSCT). Methods 30 adults with normal teeth were studied by multislice spiral CT scanner,window width and window level was 3000~5000 HU and 1200~1500 HU respectively.MSCT findings were analysed using multiplanar reformation,dental panorama and 3D reconstruction were done in all cases.Results The tooth were showed as multilaryer structures with different density at MSCT because of the mineral content of different part of tooth,from high to low density was enamel,circumpulpal dentin,outside dentin,cementum and pulp.The periodontal membrane was low density located beween the cementum and lamina dura and the thickness was homogeneous.The bone cortex of alveolar bone was successive,the bone trabecula appeared as wirelike or reticulate.Conclusion The structures of permanent teeth and alveolar bone can be displayed clearly by MSCT.Multiplanar reformation,dental panorama and 3D reconstruction are helpful to display the structures of teeth and alveolar bone as well as the relationship around structures.
7.The recognition of clinical application of glucocorticoids
Journal of Medical Postgraduates 2018;31(2):113-117
Because of the significant anti-inflammatory and immunosuppressive effects,glucocorticoids (GCs) have been widely used in clinic However,adverse reactions caused by long-term use of GCs and glucocorticoid resistance (GR) are always troublesome problems in clinical department.Therefore,it is the hot point to explore the ways to reduce adverse reactions of GCs and to improve sensitivity of GCs.In this article,we mainly discuss new strategies of reducing adverse reactions and improving sensitivity of GCs.
8.Nursing for Neurogenic Large Intestine Dysfunction
Yan CHEN ; Kun LI ; Xiuyu YANG ; Xia SU ; Xiangli ZHENG ; Yiping WANG ; Li' ; e CAI
Chinese Journal of Rehabilitation Theory and Practice 2008;14(5):500-500
Objective To study a rehabilitation nursing menus for neurogenic large intestine dysfunction.Methods Various nursing approaches were used for defecation dysfunction.Results and Conclusion 94.60% patients improved in the second week,which including gained more awareness,control,and spend less time of defecation.
9.Screening of LRRK2 interactants by yeast 2-hybrid analysis
Xiaoye ZHENG ; Mei YANG ; Jieqiong TAN ; Qian PAN ; Zhigao LONG ; Heping DAI ; Kun XIA ; Jiahui XIA ; Zhuohua ZHANG
Journal of Central South University(Medical Sciences) 2008;33(10):883-891
Objective To isolate and identify the potential binding partners of LRRK2,a gene linked to both dominant familial form and sporadic form of Parkinson's disease,thus to further our knowledge of its function.Methods We used a sequence containing full-length of COR domain and part of ROC and MAPKKK domain as bait.The bait amplified by polymerase chain reaction(PCR) was then cloned into a yeast expression plasmid pGBKT7.After being sequenced and analyzed,pGBKT7-bait was transformed into the yeast strain AH109.Western blot was performed to confirm the expression of pGBKT7-bait in AH109 yeast strain.Then human fetal brain cDNA library was trarnsformed into that yeast strain.which could express pGBKT7-bait fusion protein.The yeast strain which contained pGBKT7-bait and human fetal brain cDNA library was plated on quadruple dropout medium (SD/-Trp/-Leu/-His/-Ade)containing X-a-gal.We retested these positive colonies using 2 independent yeast strains AH109 contained pGBKT7-bait or pGBKT7,respectively.At last,these plasmids isolated from these true positive colonies were analyzed by bioinformatics.Results We obtained 9 true positive colonies,these colonies were sequenced, and we performed sequence Blast in GenBank.Three colonies of the 9 positive colonies were not in open reading-frames.Among other 6 colonies,there were known proteins including spermatid perinuclear RNA-binding protein(STRBP)and BCL2-associated athanogene 5 isoform b(BAG5),as well as unknown proteins including tyrosine phosphatase non-receptor type(PTPN23),1(3)mbt-like 3 isoform b(L3 MBTL3),RALY RNA binding protein-like isoform 1(RALYL),and Homo sapiens mRNA for KIAA1783 protein,partial cds(KIAA 1783).Conclusion True positive colonies of LRRK2 are successfully obtained by the yeast 2-hybrid.Our screened proteins may provide a new research clue for revealing biological functions of LRRK2,pathogenesis of Parkinson's disease,and other neurodegerations.
10.Analysis of clinical and pathological data in 111 infants and young children with glomerular diseases.
Guang-ling LIU ; Yuan-fu GAO ; Zheng-kun XIA ; Lianfeng ZHANG ; Yuanfeng FU ; Zhongmin FAN ; Jie FU
Chinese Journal of Pediatrics 2004;42(6):460-461
Biopsy
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Child, Preschool
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Female
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Glomerulonephritis
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pathology
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Humans
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Infant
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Kidney
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pathology
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physiopathology
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Kidney Function Tests
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Male