Objective The current study was designed to observe the efficacy and toxicity of recombinant human interleukin-11 (rhIL-11) in prevention and treatment of chemotherapy-induced thrombocytopenia in patients with acute myelogenous leukemia. Methods The total of 21 acute myelogenous leukemia patients with chemotherapy-induced thrombocytopenia(

Adolescent ; Adrenergic beta-Agonists ; therapeutic use ; Albuterol ; analogs & derivatives ; therapeutic use ; Asthma ; drug therapy ; physiopathology ; Child ; Child, Preschool ; Female ; Forced Expiratory Volume ; drug effects ; Humans ; Male ; Salmeterol Xinafoate

Objective: To develop a computer game addiction inventory (CGAI) for Chinese college students. Methods:A primary inventory made by the authors was applied to 477 college students from 8 universities in Beijing for exploratory factor analysis, and then to 405 college students for confirmatory factor analysis. Results: After exploratory factor analysis, a questionnaire consisted of 33 items were established, which had four factors: dependence and addict behavior, emotion arousal, functional impairment, and shame and un-satisfaction. These four factors could explain 55.1 of the total variance, were confirmed by confirmatory factor analysis (?~2/df=2.304; IFI=0.885; CFI=0.884; NFI=0.813; RSMEA=0.057). The internal consistency of the questionnaire was good (r=0.77~0.94), the test-retest reliability was 0.907. Criteria validity shown by higher scores in addiction group than normal control was also satisfying. Conclusion: The CGAI has good psychometric features and could be applied to college students.

This study was undertaken to examine ncNOS IR in bladder after spinal cord injury (SCI). In the meantime, we determine rats bladder ncNOS IR following intravesical instillation of capsaicin after SCI. Adult Sprague Dawley rats and guinea pigs were randomly divided into normal group, sham injury group, spinal cord injury group (4～5weeks after T 8～9 transection). A dose of 1mmol/L intravesical capsaicin was instilled in a part of SCI rats. Bladders of all animals were divided into three tissue pieces: bladder base, bladder body and bladder dome,in which the distribution of ncNOS IR was examined. Spinal transection induced a significant ncNOS IR increase in guinea pig bladder base. In rats, the quantity of ncNOS IR did not differ between the two groups. Instillation of intravesical capsaicin can cause significant up regulation of rat bladder ncNOS IR after SCI. The increase of ncNOS IR in SCI animals bladder indicates that NO may play an important role in the regulation of micturition reflex after SCI. In SCI rats, ncNOS IR can significantly be up regulated by intravesical capsaicin instillation, suggesting that NO may act as a factor in the action of capsaicin.

Abstract: This retrospective analysis examines the diagnosis and treatment of a case of chronic liver disease infected with Vibrio vulnificus, admitted to the First Affiliated Hospital of Hainan Medical University on August 8, 2023. The patient, a 34-year-old male fisherman, had chronic viral hepatitis B. He sustained a leg abrasion while fishing on August 3, and developed fever and continuous swelling pain in both lower limbs, more severe in the left limb, on the night of the injury. On the fourth day of the disease, he went to the local hospital where he received antibiotic treatment and was transferred to the First Affiliated Hospital of Hainan Medical College on August 8. Upon admission, the patient exhibited low blood pressure, high lower limb skin temperature, poor peripheral blood transport, visible scab, and signs of infectious shock. Within 12 hours of admission, an abscess incision and drainage procedure was performed on his left calf, along with negative pressure wound therapy, and tissue samples from the infected left lower limb were sent for microbial culture.The pathogen culture indicated a Vibrio vulnificus infection, and the histopathology results confirmed necrotizing fasciitis. Treatment included meropenem combined with vancomycin for infection control, maintenance of blood pressure and electrolyte balance, tenofovir disoproxil fumarate for antiviral therapy, and polyene phosphatidylcholine capsules for liver protection based on the drug sensitivity results. Additionally, levofloxacin and cefotaxime were administered for infection control and debridement was performed based on wound conditions. After 17 days of hospitalization, the infection was substantially controlled, but the liver function enzyme was still higher than the normal level, and the wound still had blood and exudation. The patient and his family members requested discharge; however, due to poor wound healing, he was readmitted on September 11, 2023, receiving antiviral and liver preservation treatment. On the fifth day,the secretion culture identified a co-infection with Corynebacterium striatum and Providencia stuartii infection. Corynebacterium striatum was subsequently reported as multidrug-resistant, prompting an immediate switch to sensitive antibiotics and supportive care. Sixteen days after readmission, the patient's general condition improved, but the liver function enzyme levels were still higher than normal, so he was discharged. Four months after discharge, a follow-up phone call revealed that the patient had actively pursued antiviral treatment and abstained from alcohol, showing reasonable wound recovery. The patient's case, with a typical exposure history and clinical manifestations, matches the characteristics of septic shock and necrotizing fasciitis caused by V. vulnificus. Clinically, it is crucial todiagnose and treat V. vulnificus infection early according to its pathological characteristics and clinical characteristics to improve the prognosis of patients.

Objective To investigate the application effect of scene exercise training model of 6 emergency disease entities in the junior nurses training .Methods Thirty‐six newly employed nurses during 2010-2013 were performed the scene exercise train‐ing of six disease entities .Before and after training ,the the forms of questionnaire investigation ,written examination and implemen‐tation were adopted to survey and analyze the post competence ,training attitude ,theoretical knowledge ,operation skill ,single time for help and participating doctor′s satisfaction on the coordinate ability .Results After training ,the aspects of post competence , training attitude ,theoretical knowledge ,operation skills ,single time for help and participating doctor′s satisfaction on the coordinate ability in junior nurse were significantly improved ,the differences between before and after training had statistical significance (P<0 .01 or P<0 .05) .Conclusion Using the scene exercise training model of 6 emergency disease entities in junior nurses training has active significance for increasing the overall quality of junior nurses .

Objective To perform the chromosome detection in 1 237 infertile patients for analyzing the karyotypes results and investigating the relationship between infertility and chromosome abnormalities .Methods The peripheral venous blood samples in 1 237 infertile patients in our hospital from March 2010 to December 2014 were collected ,performed the lymphocyte culture ,ob‐tained cells ,fixed under hypotonic condition ,prepared the section and observed them by microscope after G‐banding treatment .Re‐sults Among 1 237 patients ,111 cases abnormal karyotypes were detected with the total abnormal detection rate of 8 .9% ,in which ,57 cases were sex chromosomes abnormality ,54 cases were euchromosomes abnormality .Conclusion Chromosome abnor‐mality is one of the important causes leading to primary infertility .Infertility caused by chromosome abnormalities is irreversible ,so the chromosome cytogenetic examination is especially important in the diagnosis and treatment process of infertile patients .

Objective To compare the efficacy and predictability of sphere-cylinder-combined LASIK and two-zone cross-cylinder LASIK for the correction of moderate and high astigmatism.Design Prospective,comparative case series.Participants 40 eyes of 35 patients with mixed astigmatism undergoing LASIK.Methods All patients were treated with Visx Star IV LASIK system.20 eyes of 19 cases were used for sphere-cylinder combined LASIK mode and 20 eyes of 16 cases for two-zone-cross-cylinder LASIK mode.All sub- jects were followed more than 6 months.Main Outcome Measures Uncorrected visual acuity (UCVA),best spectacle-corrected visual acuity (BSCVA),spherical diopter,residual astigmatism and corneal thickness.Results For the patients who received two-zone-cross-cylinder LASIK mode,the UCVA at 6 months after surgery was 0.5 or above,and 13 eyes (65.0%) were 1.0 or above. For the patients who received sphere-cylinder-combined LASIK mode,the UCVA at 6 months after surgery was 0.5 or above,and 11 eyes (55.0%) was 1.0 or above (P=0.683).The residual astigmatism for the patients received sphere-cylinder-combined LASIK mode was (1.15?1.00)D,while for the patients received cross-cylinder LASIK mode was (1.13?0.62)D(P=0.045).The remotion depth of cornea for sphere-cylinder-combined LASIK mode and cross-cylinder LASIK mode was (36.73?13.12)?m and (15.60?6.85)?m,respectively (P= 0.031).Condusion The UCVA,residual astigmatism and corneal thickness after surgery in two-zone-cross-cylinder LASIK mode were better than that in sphere-cylinder-combined LASIK mode for the correction of moderate and high astigmatism.

Objective To explore the relationship between parent hostility and adolescent externalizing behavior problems and the mediating role of parenting.Methods The cross-sectional data of 4474 adolescent aged 10-21 of the National Survey of Adolescent Mental Health in China (2008) was used.Adolescents reported their externalizing problems and parents' parenting.Parents reported their hostile behaviors.Results ① Parent hostility((9.18 ± 3.56),(8.94 ± 3.18)) significantly correlated with monitoring,inductive reasoning,harshness of parenting(father(3.39 ± 1.16),(3.21 ± 1.02),(1.39 ± 0.62) respectively,r =-0.108 ～ 0.489,P ＜ 0.01 ;mother (3.96 ± 0.97),(3.38 ± 0.96),(1.37 ± 0.60) respectively,r =-0.148 ～ 0.468,P ＜ 0.01) ; and both parent hostility and parenting were significantly associated with adolescent externalizing problems (0.23 ± 0.21) (r =-0.308 ～ 0.577,P ＜ 0.01).②Parental monitoring,inductive reasoning and harshness discipline partially mediated the relations between parent hostility and adolescent externalizing problems (Z =6.12,5.10,6.36,P ＜ 0.01 ; Z =5.86,6.97,11.84,P ＜ 0.01).Conclusion Parenting partially mediated the effect between parent hostility and adolescent externalizing problems.

Objective To study the single nucleotide polymorphisms (SNP) in 3 sites allele (T189M, R85H, C121W) of SCN1B and the association between gene distribution and epilepsy. Methods All 330 blood samples of refractory (80 cases), non-refractory (100 cases) epilepsy patients and healthy people (150 cases) were collected. Genomic DNA of leucocyte was extracted. SNPs of three sites allele of SCN1B were tested by allele-specific primer-polymerase chain reaction (ASP-PCR).Data were analyzed by SAS 8.1 statistical software. Results Epilepsy group and healthy group had significantly statistical difference in composition of 3 sites allele on single site genotype (x~2=11.19, 11.14 and 6.50, all P < 0.05).There was no statistical significance between refractory and non-refractory epilepsy group. On gene combination, in 27 different combinations of polymorphism, mutation frequency in 3 sites (CT + AG + CG) was highest in epilepsy group (18.40%).The next was one site in CT + GG + CC (16.80%).In healthy group, frequency of non-variant in CC + GG + CC was highest (16.67%), and the next was 2 sites in CT+ AG+CC (13.73%).Thirty-five cases in epilepsy group (28.80%) had 3 sites mutation compared with 10 cases in healthy group (9.71%), and their difference had statistical significance (x~2=12.54, P<0.05).Eighteen cases in refractory epilepsy group (30.51%) had 3 sites mutation compared with 21 cases in non-refractory epilepsy group (28.77%), and the difference had no statistical significance. Fifty cases in epilepsy group (40.00%) had 2 sites mutation compared with 41 cases in healthy group (40.20%), and there was no statistical significance between them; 25 cases in refractory epilepsy group (42.37%) had 2 sites mutation compared with 21 cases in non-refractory epilepsy group (28.71%), and their difference had no statistical significance. Conclusions Mutation, especially multisite mutation of SCN1B is relatively likely to cause epilepsy in human. Gene distribution and combination of three sites allele of SCN1B in refractory epilepsy is close to that in non-refractory epilepsy.