Luminex technology,as a new type of biochip emerging,has many advantages,such as excellent repeatability,high flexibility,high sensitivity,etc.It can be used easily,quickly,economically to screen the gene and the protein expression changes,which contribute to the researches of molecular mechanisms involved in the development and progress of gynecological cancer and to provide new ways for the diagnosis and treatment of gynecological tumor.

Objective To explore the effects of aspirin on formation and dispersion of Pseudomonas aeruginosa (P.aeruginosa) biofilm.Methods The broth microdilution method was used to detect the minimal inhibitory concentration(MIC) of aspirin for P.aeruginosa.The anti-biofilm effects of aspirin on P.aeruginosa were determined on the microtiter plates combined with crystal violet staining.The serial dilution method for counting colony number on microtiter plate was used to explore the effects of aspirin on initial adherence of P.aeruginosa.Results The MIC values of aspirin against PAO1,PA18,PA53 and PA67 strains of Pseudomonas aeruginosa were 5,2.5,5 and 5 mg/mL respectively.Aspirin significantly inhibited the formation and dispersion of the biofilm of PAO1 and PA18 strains (t =5.65,P ＜ 0.05 and t =5.06,P ＜ 0.05 for inhibition;t =6.45,P ＜ 0.05 and t =6.26,P ＜ 0.05 for dispersion) at the concentration of 2.5 mg/mL.Similar effects were also found in the determination for PA67 and PA53 strains(t =6.45,P ＜0.05 and t =6.26,P ＜ 0.05 for inhibition;t =7.82,P ＜ 0.05;t =9.18,P ＜ 0.05 for dispersion) at aspirin concentration of 1.25 and 0.313 mg/mL respectively.Aspirin inhibited the initial adherence of P.aeruginosa at the concentration of 2.5 mg/mL(P ＜0.05).Conclusion Aspirin could significantly inhibit the initial adherence and biofilm formation of P.aeruginosa and disperse the 24 hour-formed mature bioiflm.

The incidence of venous thromboembolism(VTE) including deep venous thrombosis(DVT) and pulmonary embolism(PE) increases exponentially with age. Age is an independent risk factor, acute and chronic diseases and factors affecting blood coagulation and anticoagulation equilibrium are also associated with VTE. Homocysteine may be an independent risk factor for occlusive vascular disease. The author summarized the risk factor, mechanism of action, diagnosis and therapy of VTE.

Objective:To explore the factors influencing colonization of Mutans streptococci (MS) on teeth of children.Methods:Dental colonization of MS was investigated in 123 children aged from 7 to 42 months in Beijing. Oral examination was performed. Information of nursing habits, diet history etc. was obtained from parent questionnaires.Results:Logistic regression analysis showed that the number of erupted teeth and the intake of sugar-containing food were positively related with MS colonization, their WALD values were 17.411(P=0.000) and 5.637(P=0.018) respectively,OR values were 3.287 and 2.739 respectively.Conclusion:The main factors influencing the colonization of MS were the number of erupted teeth and the intake of sugar-containing food.

Objective To explore the effect of the prenatal dexamethasone treatment on the preventionof wet lung syndrome in the newborn of elective caesarean section at term. Methods Two hundred cases of elective caesarean section at term (the gestation age was less than 39 weeks) were divided into observation group and control group,with 100 cases in each by random digits table. Observation group was treated with four intramuscular injections of 6 mg dexamethasone in the 24 hours to 7 days before delivery, and control group was treated with 0.9% sodium chloride injection with the same method. The clinical data and the incidence of the wet lung syndrome of the newborn were collected. Results The incidence of the wet lung syndrome of the newborn was 1%(1/100) in observation group and 9%(9/100) in control group,and the incidence of the severity of the wet lung syndrome of the newborn was 1% ( 1/100) in observation group and 2%(2/100) in control group. There were no complication in observation group and 1 case in control group.The incidence and the severity of the wet lung syndrome of the newborn in observation group were less than that in control group significantly (P = 0.009,0.049). Conclusion Prenatal dexamethasone treatment can reduce the incidence of the wet lung syndrome of the newborn and decrease the incidence of the severity and complication of elective caesarean section at term.

The process of liver metastasis of colorectal cancer includes tumor cells invasion,survival in blood stream,extravasation to the liver and proliferation. It is involved in series of molecular markers,among which vascular endothelial growth factor and endothelial growth factor receptor are the major targets.

Anhedonia, or markedly diminished interest or pleas-ure, is a hallmark symptom of depression.As a psychopathological symptom, anhedonia was first noted in the early 19th century.The neurobiological mechanisms that underline anhedonia and its role in diagnosing depression disorder or evaluating antidepressant response have long been aroused attention for nearly a century.Consequently, there are many measuring methods established in both the animal study and human research, which would be reviewed in the present study.

Objective To investigate the molecular biological mechanism of deposition of triglyceride(TG)in hepatocytes in alcoholic fatty liver disease(AFLD)and the pathogenesis of this condition by detecting the contents of serum tumor necrosis fac-tor-α(TNF-α),liver triglyceride(TG),peroxisome proliferator-activated receptorα(PPARα)and acyl-CoA oxidase(Acox1)mR-NAs,and liver PPARαprotein after intervention with bezafibrate,a PPARαagonist.Methods Sixty Wistar rats were randomly divided into three groups:control group(n=20),AFLD group(n=20),and bezafibrate group(n=20).Animals in control group were given distilled water by gavage once a day for 8 weeks.Those in AFLD group were given ethanol and fish oil(2.5 mL/kg) by gavage daily for the same period of time.In bezafibrate group,rats were treated by gavage with ethanol and fish oil(2.5 mL/kg)for the first 4 weeks and then with bezafibrate(100 mg/kg)for another 4 weeks.TG in the liver was measured by colorimet-ric method,serum TNF-αlevels by enzyme linked immunoabsorbent assay (ELISA),the mRNA expression of PPARαand Acox1 in hepatocytes by reverse transcription polymerase chain reaction(RT-PCR)and the expression of PPARαprotein in hep-atocytes by Western blot.Results A significant increase in TG[AFLD group(0.72±0.09)mmol/L vs.control group(0.28± 0.07)mmol/L,P<0.01]and TNF-α[AFLD group(3.01±0.31)ng/mL vs.control group(1.07±0.28)ng/mL,P<0.01]was found in AFLD group when compared with control group.After bezafibrate intervention,the contents of liver TG and serum TNF-αwere significantly decreased.The mRNA expression of PPARα[AFLD group(0.22±0.08)vs.control group(0.68± 0.13),P<0.01]and Acox1[AFLD group(0.43±0.12)vs.control group(1.14±0.21),P<0.01]was suppressed in AFLD group,which was significantly reversed by bezafibrate treatment[bezafibrate group(0.59±0.13)for PPARαmRNA vs.AFLD group,P<0.01;bezafibrate group(0.83±0.17)for Acox1 mRNA vs.AFLD group,P<0.01].The expression of PPARαpro-tein in hepatocyts was also found to decrease in AFLD group[AFLD group(0.19±0.07)vs.control group(0.48±0.11),P<0.01].After bezafibrate intervention,it was profoundly increased.Conclusion The down-expression of PPARαand Acox1 in the liver of rats with AFLD may suppress the fatty acid metabolism and lead to the TG deposition in the liver.The increase in serum TNF-αcontents also contributes to the development of AFL.Bezafibrate can prevent and treat AFL by activating PPARα,increasing the expression of PPARαand Acox1 ,promoting the metabolism of fatty acids,decreasing the TG deposition and the serum TNF-αcontents.

Objective To investigate CT findings of atypical pheochromocytoma.Methods 18 cases of atypical pheochromocytoma were verified with operation and pathology.2 cases were extra-adrenal pheochromocytoma,3 cases were bilateral or multiple pheochromocytoma,7 cases were non-function pheochromocytoma and malignant pheochromocytoma were 6 cases.Results All atypical pheochromocytoma showed equivalent density mass with necrosis in CT scanning,calcification was rare.Most of them had strong enhancement in parenchyma.Conclusion (1)Atypical pheochromocytoma has high tendency to malignance.(2)CT has high locational and qualitative rate in diagnosing atypical pheochromocytoma but can not different malignance from benign pheochromocytoma,except the mass has metastatic signs.The diagnosis of non-function pheochromocytoma has to consider biochemical and other examination.