Objective:To compare the rat models of ulcerative colitis induced by 2,4,6-trinitro-benzene-sulfonic acid(TNBS) and immune-comb method.Methods:SD rats were randomly distributed into groupⅠ(immune-comb method),groupⅡ(TNBS/ethanol),groupⅢ(50% ethanol) and groupⅣ(control).The rats in groupⅠwere sensitized 2 times by antigen made of rabbit colon mucosa in 2-week intervals.After abrosia for 24 hours,each rat in four groups received enema of 0.65 ml different fluids,which were:TNBS 100mg/kg +50%ethanol for groupⅠand Ⅱ,50%ethanol for group Ⅲ,and normal sodium for control group.Rats were sacrificed on day 1,21 and week 8,12 after enema to observe the macroscopical focus of infection in bowels and the microscopical changes.Results:The typical erosion and ulcer changes appeared in groupⅠandⅡ,and there was accompanying pathological changes in the end piece of ileum in groupⅠ.The pathological changes for groupⅠcould keep for 8 weeks,while those for group Ⅱtrended to heal 3 weeks later.The pathological changes in groupⅢonly were hyperemia,oedema and a few anabrosis or superficial ulcer,keeped for 21days.There was no tissue damage in group Ⅳ.Conclusion:Immune-comb method is comparatively the ideal method to establish the model,,because it has a good reproducibility,a long persistence time,and the pathological changes resemble more to those of man..

Objective:To investigate the transference of transplanted bone marrow mesenchymal stem cells (BMSCs)in rat of ulcerative colitis. Methods:SD rats were randomly distributed into group A colitis induced by immune-combined TNBS/ethanol and group B as control. All rats received caudal vein injection with 1 ml of fluorescence marked BMSCs,then 5 rats were sacrificed at day 3,7 and 14 respectively in each group. Cryostat sections of bone marrow,lung,liver,spleen and gut were made to observe the distribution of BMSCs in these organs,and the fluorimetric integral optical density(IOD)were analyzed. Results:BMSCs were mainly distributed in organs which richs vessels. The highest IOD level of BMSCs in lung was on the 7th day,and was on the 14th day in bone marrow. On the 14th day,the IOD level of BMSCs in colon of group A were higher than those of other segments of digestive tube and also higher than those in group B (P

Objective To investigate the inhibitory effect of celecoxib on non-small cell lung cancer in vitro and the role of survivin.Methods The inhibitory effect of celecoxib was detected by MTT.Flow cytometry and electron microscope were used for evaluation of apoptosis.The expression of survivin was detected by RT-PCR and Western blot.Results ①Celecoxib inhibited cell survives in a time-dependent and concentration-depended manner,and the effect of celecoxib was more significant in NCI-H520 than in A549.②Celecoxib caused apoptosis in concentration-depended manner.3.Celecoxib decreased the expression of survivin.Conclusion The decrease of the expression of survivin may play a critical role in the apoptosis of NSLLC in vitro induced by celecoxib.

Animals ; Dogs ; Drug Evaluation, Preclinical ; methods ; Statistics as Topic

Objective To observe the effect of PKB gene silencing on the growth of gastric cancer cell line SGC-7901 in vitro.Methods Gene transfection technique was used to transfect AKt2 siRNA into gastric cancer cells.Akt2 expression was detected by RNAi technique,Akt2 protein level was detected by Western blot,and the change of cell cycle distribution and apoptosis of SGC-7901 cells were detected by flow-cytometry,SGC-7901 proliferation was measured by MTT method.Results After SGC-7901 cells transfected with Akt2 siRNA,the expression of protein level decreased obviously(P

Hepatitis C, Chronic ; Humans

Objective To investigate the early diagnosis and rational treatment of traumatic retroperitoneal hematoma(TRPH). Methods Retrospective analysis was done on the clinical data of 96 cases of TRPH admitted in our hospital in the past 10 years. Results Eighty two patients received operation, of whom,73 combined with other celiac organ injuries.The other 14 patients were treated by non surgery.Two patients combined brain trauma and 1 with hemorrhagic shock died during operation,and 2 died of multiorgan failure postoperatively. Conclusions Early diagonsis depends on clinical appearance, abdominocentsis, B ultrasonography and CT. Operation should be done immediately,if the patient has the following condition:(1)apparant hemorrhagic shock after blunt trauma;(2) comfirmed celiac viscera trauma or blood vessel trauma;and (3)retroperitoneal hematoma caused by penetric trauma. Kidney surrounding hematoma or hematoma in pelvis area needs no immediate operation.

[Objective]To investigate the capability of synthesis BMP-2-derived peptide on osteogenic induction in bone marrow stem cells(BMSEs),and to evaluate the osteoinductivity of BMP-2-derived peptide in vitro.[Method]After segregating and cultivating four weeks Wistar rats marrow stromal cells in induction group were induced by osteogenasis medium containing 200 gg/ml BMP-2-derived peptide,and in non-inductional group BMSCs were still culture with DMEM medium.Following continue culture for 2~4 weeks,cells film preparation,alkaline phosphatase activity and calcium deposition were measured.Type I collagen(CoM)and osteopontin(OPN)mRNA expression were measured using real-time fluorescent quantitative polymerase chain reaction(FQ-PCR)technique.The capability of synthesis BMP-2-derived peptide on osteogenic induction of BMSCs was investigated.[Result]After inductived with BMP-2-derived peptide.BMSCs cultured survived well greatly changed in cell morphology,and showed a biological and morphologie characteristics similar to those of osteoblasts.The lever of alkaline phosphatase activity and calcium deposition increased.Col-Ⅰand OPN mRNA were expressed at higher level.In non-inductional group no conspicuous osteogenic induction was shown.[Conclusion]BMP-2-derived peptide can induce BMSCs to differentiate into osteoblasts.It has the similar capacity of osteogenic induction as nature BMP-2,so BMP-2-derived peptide is an ideal cell agent for bone tissue engineering,and can be available widely.

Objective To observe the effects of VEGF antisense oligonucleotide transfected to SGC-7091 cell line on expression of VEGF and survivin of the cell.Methods The VEGF-ASODN was synthesised artificially.There were five groups in this experiment: control group,missense group and three various concentration groups.After transfection,RNA copies were detected by real-time PT-PCR,VEGF protein in cell and culture supernate was detected by ELESA,survivin protein was measured by Western-blot,and apoptosis was detected by FCM.Growth of cells was evaluated by growth curve.ResultsVEGF-ASODN tranfection reduced the VEGFmRNA and VEGF protein expression in gastric cancer cells and supernate remarkably.It also reduced survivin protein expression and increased apoptosis in gastric cancer cells.Conclusions Transfection with VEGF-ASODN to gastric cancer cells SGC-7901 can reduce the expression of VEGF,and can increase apoptosis and suppress the proliferation of gastric cancer cells.

Correlation between TCM typing of chronic gastritis and the findings about the mucosa in gastroscopic examination was analyzed. In GLM and DUNCAN analyses, the sufferers of insufficiency of both the spleen and stomach were much older than of the damp - heat in the spleen and the stomach (P