Objective To investigate the inhibitory effect of celecoxib on non-small cell lung cancer in vitro and the role of survivin.Methods The inhibitory effect of celecoxib was detected by MTT.Flow cytometry and electron microscope were used for evaluation of apoptosis.The expression of survivin was detected by RT-PCR and Western blot.Results ①Celecoxib inhibited cell survives in a time-dependent and concentration-depended manner,and the effect of celecoxib was more significant in NCI-H520 than in A549.②Celecoxib caused apoptosis in concentration-depended manner.3.Celecoxib decreased the expression of survivin.Conclusion The decrease of the expression of survivin may play a critical role in the apoptosis of NSLLC in vitro induced by celecoxib.

Objective:To compare the rat models of ulcerative colitis induced by 2,4,6-trinitro-benzene-sulfonic acid(TNBS) and immune-comb method.Methods:SD rats were randomly distributed into groupⅠ(immune-comb method),groupⅡ(TNBS/ethanol),groupⅢ(50% ethanol) and groupⅣ(control).The rats in groupⅠwere sensitized 2 times by antigen made of rabbit colon mucosa in 2-week intervals.After abrosia for 24 hours,each rat in four groups received enema of 0.65 ml different fluids,which were:TNBS 100mg/kg +50%ethanol for groupⅠand Ⅱ,50%ethanol for group Ⅲ,and normal sodium for control group.Rats were sacrificed on day 1,21 and week 8,12 after enema to observe the macroscopical focus of infection in bowels and the microscopical changes.Results:The typical erosion and ulcer changes appeared in groupⅠandⅡ,and there was accompanying pathological changes in the end piece of ileum in groupⅠ.The pathological changes for groupⅠcould keep for 8 weeks,while those for group Ⅱtrended to heal 3 weeks later.The pathological changes in groupⅢonly were hyperemia,oedema and a few anabrosis or superficial ulcer,keeped for 21days.There was no tissue damage in group Ⅳ.Conclusion:Immune-comb method is comparatively the ideal method to establish the model,,because it has a good reproducibility,a long persistence time,and the pathological changes resemble more to those of man..

Objective:To investigate the transference of transplanted bone marrow mesenchymal stem cells (BMSCs)in rat of ulcerative colitis. Methods:SD rats were randomly distributed into group A colitis induced by immune-combined TNBS/ethanol and group B as control. All rats received caudal vein injection with 1 ml of fluorescence marked BMSCs,then 5 rats were sacrificed at day 3,7 and 14 respectively in each group. Cryostat sections of bone marrow,lung,liver,spleen and gut were made to observe the distribution of BMSCs in these organs,and the fluorimetric integral optical density(IOD)were analyzed. Results:BMSCs were mainly distributed in organs which richs vessels. The highest IOD level of BMSCs in lung was on the 7th day,and was on the 14th day in bone marrow. On the 14th day,the IOD level of BMSCs in colon of group A were higher than those of other segments of digestive tube and also higher than those in group B (P

Animals ; Dogs ; Drug Evaluation, Preclinical ; methods ; Statistics as Topic

Objective To observe the effect of PKB gene silencing on the growth of gastric cancer cell line SGC-7901 in vitro.Methods Gene transfection technique was used to transfect AKt2 siRNA into gastric cancer cells.Akt2 expression was detected by RNAi technique,Akt2 protein level was detected by Western blot,and the change of cell cycle distribution and apoptosis of SGC-7901 cells were detected by flow-cytometry,SGC-7901 proliferation was measured by MTT method.Results After SGC-7901 cells transfected with Akt2 siRNA,the expression of protein level decreased obviously(P

Hepatitis C, Chronic ; Humans

Objective To observe the therapeutic effects and complication of combined endoscopic trerapy of the acute biliary pancreatitis(ABP). Methods 70 cases of acute biliary pancreatitis were treated in our hospital in recent 7 years.37 cases(group E) admitted undergone emergency endoscope procedure were treated with endoscopi- cretrograde cholongiopancreatography ( ERCP ), endpscopic sphincterotomy ( EST ) and endoscope naso-biliary drainage(ENBD) in 24h after be hospitalized. 33 cases(group C) were treated with conservation treatment or opera-tion treatment. Results Achievement ratio of endoscopic management was 92 %. There was no severe complication or mortality in group E. The duration of symptoms and hospitalization in endoscopy group were significantly shorter than those in group C(P<0.01). The change of serum and urine amylase was not significant between two groups in the first day after treatment(P > 0.05). But the serum and urine amylase of group E were significantly lower than those in group C in the second day(P< 0.05) and more significantly lower in the third day after treatment(P< 0.01 ). Conclusion Early endoscopic therapy is safe and effective in ABP patients. It is a valuable method to treat a- cute biliary pancreatitis.

BACKGROUND: Biliary ischemia-reperfusion injury is one of the main reasons for the injury bile duct following liver transplantation, liver resection and hepatic artery thrombosis after chemotherapy. However, nothing has been decided yet concerning liver can tolerate long intrahepatic bile ducts ischemia under normal temperature. OBJECTIVE: To use the rabbit biliary ischemia-reperfusion injury, model, analyze the rabbit intrahepatic bile ducts ischemia security time. METHODS: Rabbits were randomly divided into sham operation, hepatic artery and common bile duct joint blocking 1.5, 2, 2.5, 3 h groups. Sham operation group only free common bile duct, hepatic artery and portal vein. Hepatic artery and common bile duct joint blocking 1.5, 2, 2.5, 3 h groups, left and right hepatic duct openings superior margin was clamped using artery clamps, and hepatic artery, common bile duct and loose connective tissue were occluded. Following 1.5, 2, 2.5 and 3 hours, artery clamps were removed to recover hepatic artery or biliary tract blood flow. After a week, animal survival was calculated and liver function was tested. RESULTS AND CONCLUSION: None animals in the sham operation and hepatic artery and common bile duct joint blocking 1.5 hour groups died;survival rate was 87.5% following 2 hours of blocking. Animal survival rate was decreased gradually with prolonged blocking time of blood flow. These indicated that the maximum safety time of blocking biliary duct blood flow was 2 hours. Pathological and histological changes were mild within 2 hours of blocking, mainly presenting cell edema and inflammatory cell infiltration, and necrotic focus was punctiform or fragmentis, reversible. While ischemia above 2 hours, bile duct epithelial necrotic shape was significant. The obvious, hepatic necrosis was multifocal, flake, irreversible damage. Histological change also confirmed that 2 hours may be maximum security limitation of rabbit tolerance intrahepatic bile duct flow blocking under normal temperature.

Objective To evaluate the key points of anesthetic management for retroperitoneoscopic nephrectomy combined with inferior vena cava ( IVC ) tumor thrombectomy. Methods Perioperative clinical data of 3 patients undergoing retroperitoneoscopic nephrectomy combined with IVC tumor thrombectomy from December 2010 to June 2014 were retrospectively analyzed.There were 2 cases of level Ⅰthrombus and 1 case of level Ⅱthrombus.All the patients were given general anesthesia with intubation.Brief inferior vena cava occlusion was performed in the patient with level Ⅱ thrombus. Results The operation was completed smoothly in all the cases without conversions to open surgery. The operating time was 244, 333, and 289 min, respectively. The total IVC control time for level Ⅱ thrombus was 10 min.No intraoperative pulmonary embolism and other severe anesthetic complications occurred. One patient with level ⅠIVC thrombus was extubated and sent back to surgical ward after surgery, and the remaining 2 patients underwent intensive care monitoring overnight and were extubated and discharged to surgical ward on the next day. Conclusions Retroperitoneoscopic nephrectomy and IVC thrombectomy is a brand-new, difficult but feasible procedure. Anesthesiologist should have a full recognition of surgical procedures and make perfect anesthetic plan to ensure close collaboration. Furthermore, circulatory swing during IVC clamping and perioperative severe complications such as pulmonary embolization and massive hemorrhage cannot be neglected.

Objective To observe the effects of VEGF antisense oligonucleotide transfected to SGC-7091 cell line on expression of VEGF and survivin of the cell.Methods The VEGF-ASODN was synthesised artificially.There were five groups in this experiment: control group,missense group and three various concentration groups.After transfection,RNA copies were detected by real-time PT-PCR,VEGF protein in cell and culture supernate was detected by ELESA,survivin protein was measured by Western-blot,and apoptosis was detected by FCM.Growth of cells was evaluated by growth curve.ResultsVEGF-ASODN tranfection reduced the VEGFmRNA and VEGF protein expression in gastric cancer cells and supernate remarkably.It also reduced survivin protein expression and increased apoptosis in gastric cancer cells.Conclusions Transfection with VEGF-ASODN to gastric cancer cells SGC-7901 can reduce the expression of VEGF,and can increase apoptosis and suppress the proliferation of gastric cancer cells.