Malignant bowel obstruction (MBO) is common in patients with bowel or gynaecological canc-er. Although surgery is the primary treatment for most patients with MBO, it should not be undertaken rou-finely in patients with poor prognosis. Nasogastric drainage is only a temporary measure. Serf-expanding me-tallic stents are optimistic in malignant obstruction of the gastric outlet and proximal small bowel.

Gastrointestinal stromal tumours(GISTs)are the most common primary mesenchymal tumours of the gastrointestinal tract and the disease is occasional and asymptomatic. Though the high rate of recur- rence after surgery, operation remains the firstline treatment of the GIST without metabasis. With the consid- eration the molecular biology of tumor, the treatment including surgery and molecular targeted therapy can im- prove the prognosis of GISTs.

Objective To investigate the association between diabetes and pancreatic cancer to provide the basis for the early di‐agnosis and prevention of pancreatic cancer .Methods By formulating the retrieval stategy ,the databases of PubMed ,EMbase ,Ov‐id 、EBSCO ,CNKI and CBMdisc ,VIP ,Wanfang/database etc .were retrieve by computer from their establishment to July of 2013 .At the same time ,the relevant literature references were also retrieved .All case‐control studies(CCS) on the association between diabe‐tes and pancreatic cancer were included and their quality was assessed .The meta analysis was conducted by using Review Manager 5 .0 software .Results A total of 21 CSC were included ,finally 18 case‐control studies met the inclusion criteria for conducting meta analysis ,including 6 184 cases in the pancreatic cancer group and 10 296 cases in the control group .The meta analysis on the pan‐creatic cancer group and the control group showed that the result of heterogeneity test from all research data was I2 = 74% ,χ2 =65 .38 ,P< 0 .01 ,the random effects model was adopted ,the pooled odds ratio(OR) was 3 .60 and 95% confidence interval(95% CI) was 3 .28 - 3 .94 ,the difference showed the statistic significance(P< 0 .05) ,which indicating that the risk suffering diabetes in the patients with pancreatic cancer was 3 .6 times of the normal population .Conclusion Diabetes is associated with pancreatic cancer and diabetes may be an early clinical manifestation of pancreatic cancer .

Objective To explore the effects of psychological intervention on negative emotions and quality of life in patients with rheumatoid arthritis (RA).Methods A total of 112 patients with rheumatoid arthritis were divided into two groups:the intervention group and the control group.All patients were given drugs for treatment of RA.Additionally,patients in the intervcntion group were received psychological intervention.Zung's self-rating depression scale (SDS),Zung's self-rating anxiety scale (SAS),RA disease activity score (DAS28),visual analog scale (VAS),disability index of health assessment questionnaire (HAQ-DI) were assessed,and the related laboratory parameters such as erythrocyte sedimentation rate (ESR),C reactive protein (CRP),rheumatoid factor (RF)were determined.Results 6 months after the treatment,there were significant differences in SDS and SAS between the treatment group and the control group [SDS:(31.7±4.7)scores vs.(29.6±4.4)scores; SAS:(34.7±5.3)scoresvs.(32.6±5.1)scores; t=2.441,-2.119,both P＜0.05].The patients with tender joints,joint swelling and morning stiffness time were less in the treatment group than in control group [(5±3) vs.(7±4),(4±3) vs.(5±3),(36±16) min vs.(42±19)min.respectively,t=2.054,2.393,1.995,all P＜0.05].The scores of HAQ-DI,VAS and DAS28were lower in the treatment group than in control group [(0.89±0.73) vs.(1.20±0.77),(6.1±1.6) vs.(6.8±1.8),(3.2±1.7) vs.(3.9±1.9),respectively,t=2.136,2.406,2.135,all P＜0.05].There was a significant difference in CRP between the treatment group and the control group [(34±14) mg/L vs.(37± 17)mg/L,t=2.406,P＜0.05].Conclusions Psychological intervention can improve the negative emotions and quality of life in patients with rheumatoid arthritis.

Objective To observe the effect of combination therapies with methotrexate, sulfasalazine and hydroxychloroquine on hyperlipemia of rheumatoid arthritis.Method From 2009 November to 2010 November, 68 RA inpatients and outpatients in the department of rheumatism of our hospital were randomly divided into two groups: A group and B group, 34 cases in each group. The patients in A group were given combination of methotrexate, sulfasalazine, hydroxychloroquine sulfate for the treatment of RA,patients in B group, based on A group's treatment, were treated by xuezhikang. Some indexes were observed in two group before treatment and 6 months after treatment, including cholesterolTC,low density lipoprotein cholesterol LDL-C , triglyceride TG , high density lipoprotein cholesterol HDL-C , swelling index, joint pain index, time of morning stiffness, C-reactive protein CRP , erythrocyte sedimentation rate ESR and DAS28. Results After 6 months’ treatment, the serum levels of TC, TG,, LDL-C, both in two groups of patients, were lower than those before treatment, and HDL-C was higher than that before treatment, there were significant differences (P<0.05 or P<0.01) . After 6 months of treatment,DAS28,ESR,CRP,joint tenderness,joint swelling and morning stiffness time, both in two groups RA patients, significantly reduced compared with before treatment, there were a very significant difference (P<0.05 or P<0.01) .After treatment, the above indexes had no statistically significant difference between two groups (P>0.05) . Conclusion RA patients have abnormal blood lipid levels, disease-modifying anti-rheumatic drugs have effect on blood lipid during remission joint disease in RA patients.

Objective To develop a new type of gene vaccine against plague. Methods caf1 gene of caf Operon Encoding F1 Antigen of Y. pestis was inserted into pHSS6-mTn-3xHA/lacZ library plasmids, the recombinant plasmids were linearized with Not I and transformed into yeast cell by lithium acetate (LiAc) method to induce homologous recombination. Positive recombinants were then selected with uracil-lack medium. Results These recombinants were confirmed by colony PCR to have the target gene fragments. Conclusion The present study provided a platform for constructing a novel expression system for expressing Y. pestis F1 Antigen via homologous recombination in Saccharomyces cerevisiae, which may contribute to the genetic prevention of plague through digestive-tract-route (DTR).

BACKGROUND: Generally, repairing the injuries of sketetal muxcle is to suture the epimysial and exterior muscular nerve. OBJECTIVE: To determine the intramuscular nerve effects on the recovery of lacerated musculus rectus femoris. DESIGN, TIME AND SETTING: The randomized control animal experiment was performed at the Animal Experimental Center, Chongqing Medical University from September 2006 to August 2007. MATERIALS: Twenty New Zealand rabbits weighing 2.0-2.5 kg were used to establish models of right skeletal muscle injury; left side was used as controls. METHODS: Twenty rabbits were randomly divided into two groups. Rabbits in the intact intramuscular nerve group underwent complete muscle laceration with intramuscular nerve intact. Musculus rectus femoris was traversed at 4.0-5.0 mm from nerve entry point. Rabbits in the intramuscular neurotmesis group underwent complete muscle laceration, but intramuscular nerve was cut concomitantly at the same place as above. MAIN OUTCOME MEASURES: 28 weeks after surgery, wet weight and isometric contraction of musculus rectus femoris were detected. Morphological change of musculus rectus femoris was observed by Hematoxylin & Eosin Staining. Fibrous degree was measured by Masson trichrism staining. Myofibrillar type was examined by myosin ATPase staining and tetrazolium staining method of reduced coenzyme Ⅰ to calculate cross-sectional area of muscle fiber. RESULTS: Compared with the controls, wet weight and isometric contraction of musculus rectus femoris reduced in the intramuscular neurotmesis group (P

OBJECTIVE To investigate changes in Beijing dentists′ infection control(IC) practices in 2000 vs 2005.METHODS Data from questionaires of 647 dentists in 2000 and 592 dentists in 2005 were compared by using descriptive statistics and Person′s ?2.RESULTS Their response rates were 95.00%(2000) and 93.00%(2005).There were improvements in 2005 vs 2000 in reports of routine use of gloves(94.59% vs 73.31%);masks(56.78% vs 13.94%);and protective gown(38.87% vs 14.51%);change of gloves after each patient(93.47% vs 63.25%);vaccination for hepatitis B virus(HBV)(73.60% vs 32.66%);autoclave sterilization of dental handpieces after each patient(95.07% vs 41.24%);flushing waterlines after each use(70.78% vs 42.01%);and high-volume suction(44.92% vs 11.19%).CONCLUSIONS The compliance with recommended infection control procedures among Beijing dentists is improving in 2005 vs 2000.More education and inspection is needed to develop the infection control practices in dental health care settings.

OBJECTIVE To investigate the application of low temperature hydrogen peroxide gas plasma sterilization in dental handpieces.METHODS Totally 240 pieces dental handpieces were divided into 8 groups in accordance with different ways of oiling and package,sterilized by Johnson STERRAD 100S low temperature hydrogen peroxide gas plasma sterilizer,and then inspected by sterility testing,rotational speed and appearance observation.RESULTS The result of sterility testing of dental handpieces in auto oiling group with 4 types of packages was negative,and the sterilization effect was reliable.After 10 cycles of low temperature hydrogen peroxide gas plasma sterilization,the rotational speed of dental handpieces had no obvious decline and the appearance was quite intact without any corrosion.CONCLUSIONS The sterilization effect is reliable and the damage caused by low temperature hydrogen peroxide gas plasma sterilization on dental handpieces is not found.