The paper analyzes the urgency of constructing the medical information consulting service system in big data era as well as the features of the system,describes the architecture of the system from the perspectives of function,process relation and technology,introduces the construction of the mobile clients of the system,discusses the promotion and application of mobile clients.

Animals ; Brain ; drug effects ; metabolism ; Male ; Mice ; Mice, Inbred Strains ; Morphinans ; pharmacology ; Morphine Dependence ; metabolism ; Nitric Oxide Synthase Type I ; metabolism

Clinical symptoms with serum theoph ylline level above normal therapeutical range were analysed in 32 case. Methods: Theophylline 20μgmL-1 invserum was defined as the upper limit of normal therapeutical range. The case records with serum theo phylline concentraed ≥20μgmL-1 were selected from 324 cases, analyse d in combination with clinical symptoms. Results: Patients with serum theophylline concentration ≥20 μgmL-1 showed toxic reacti ons involving cardiovasucular, digestive, and nervous systems. One third of them took simultaneously other drugs which could decrease the metabolism of theophyl line. Conclusion: In order to prevent and diagnose toxicr eactions effectively, it's necessary to monitor the patients'serum theophylline level.

AIM The purpose of this study was to investigate the effect of immunosuppression on the periapical inflammation by estimating the expression of the cytokine IL-1? and TNF-? in periapical lesion of rat. METHODS Sixteen SD rats received cycolophosphamide injection weekly before pulp exposure for three weeks, another 16 animals received no medication as control group. The expressions of IL-1? and TNF-? mRNA in periapical lesion were detected by methods of in situ hybridization,and semi-quantified analyzed compared with control group. RESULTS IL-1? and TNF-? mRNA expressions in treated group increased progressively from 1 to 3 week, and declined somewhat at 4 week. There was a statistically significant positive correlation between the area of the periapical lesion and the number of positive stained cell for IL-1? and TNF-?(IL-1?:r=0 881,P

We hypothesized that the concentrated urea and NaCl solutions may opened the blood-brain barrier to the horseradish peroxidase and trypan blue-albumin complex by shrinking barrier cells and opening up spaces between them. The experiments were carried out on 30 healthy, adult rats. Two experimental groups were used. First, intracarotid perfusion of anesthetized rats were prepared by exposing and catheterizing the left common carotid artery. A test solution of 3.4 M, 3.0 Osm urea and 0.87 M, 1.6 Osm NaCl, was perfused manually for 30 sec. in a cranial direction so as to expel the blood from the pial arterioles of the exposed brain. The pressure, which was not measured, varied between what was required which to expel blood from both arterioles and venules. Five milliliters of the test solution usually were used. Horseradish peroxidase and trypan blue was injected intravenously or through the carotid artery after perfusion. Threshold of barrier damage due to the intracarotid substance was defined as the lowest osmotality which produced obvious blue staining of the brain both on surface observation and coronal section. The effect of a substance was defined as reversible if a threshold concentration did not produce blue staining when the dye was injected 30 min following perfusion. Second, we applied a concentrated solution of 3.0 Osm urea and 1.6 Osm NaCl to the pia-arachnoid of the cerebral cortex, to study the barrier to the intravascular horseradish peroxidase and trypan blue-albumin complex. Hyperosmotic solution of 3.0 Osm urea and 1.6 Osm NaCl, either infused into one internal corotid artery or applied topically to the pia-arachnoid surface of the brain of rats, results in the opening of endo thelial tight junction through which horseradish peroxidase passes from blood to the basal menbrane and astrocytes and neurons. The evidence for this opening of the blood-brain barrier to protein is the entry of peroxidase into the neurons. It was postulated that sufficiently high concentrations of electrolytes or relatively lipid-insoluble non-electrolytes such as urea, osmotically pulled water from the cerebral endothelial cells resulting in their shrinkage. The shrinkage, in turn, was believed to open the tight junction between continuous endothelial cells so that the dyeprotein complex could pass through the junction from blood to neurons. The present study shows that these tight junctions, the sites of the barrier to neuron movement of protein, are indeed opened by the osmotic action of urea or NaCl.

Objective To investigate the regulation effect of bispectral index (BIS) on the depth of total intravenous anesthesia (TI-VA) by comparing with regulating the depth of anesthesia according to the changes of hemodynamics. Methods 60 ASA Ⅰ～Ⅱ patients undergoing laparoscopic cholecystectomy (LC) were randomly assigned into controlled group (group C) and trial group (group T) ,with 30 patients in each group, according to the rules of regulating the titration of propofol during TIVA. The target of group C was to keep SBP/DBP 100 ～ 140mmHg/60 ～ 89mmHg and that of group T was to keep BIS 40 ～ 60 during anesthesia. The measure indexes included SBP/DBP, HR, BIS, total dosis of Propofol, time to extubate, time to leave operating room and OAA/S (observer's assessment of alert-ness/sedation). After operation, we evaluated whether awareness during surgery happens. Results As compared with group C, there were higher SBP/DBP and BIS in group T after induction of anesthesia, during aeroperitonia, immediately after finishing operation and just before extubating endotracheal catheter, but less dosis of propofol and less time of extubation and leaving operating room in group T. The differences had significance (P <0.01). There were no significant differences in the changes of HR between two groups (P >0.05) and no awareness during surgery happened in both groups. Conclusion TIVA regulated by BIS during LC can decrease the dosis of propofol, accelerate the recovery from anesthesia and avoid extremely deep anesthesia and awareness during surgery.

Objective To investigate the practical application values of copper sulfate method and hemocyte analyzer in blood do‐nation screening .Methods A total of 1 500 blood samples of donors were randomly collected and determined by using both copper sulfate method and hemocyte analyzer .Taking hemocyte analyzer as the reference method ,the sensitivity and specificity of copper sulfate method for detecting hemoglobin(Hb) were calculated .The overall detection rates of abnormal blood samples determinded by the two methods were calculated ,as well .Statistical comparisons were performed on the test data from both methods .Results A total of 29 donors(accounted for 1 .9% ) with unacceptable Hb value were found by using copper sulfate method .For all blood do‐nors ,the sensitivity and specificity of copper sulfate method for Hb screening before blood donation was 99 .7% and 82 .8% ,respec‐tively .And there were no statistically significant differences in sensitivity and specificity between the two methods for Hb estima‐tion(P>0 .05) .There were 336 donors with abnormal blood samples detected by using hemocyte analyzer .Taking hemocyte analy‐zer as the reference method ,only 8 .6% of donors with abnormal blood samples were screened out by using copper sulfate method . Conclusion The copper sulfate method could be used for Hb screening before blood donation .While because of its limitations ,such as relatively low specificity and inadequate screening items ,the hemocyte analyzer shoud be utilized before blood donation if the con‐ditions permit .

Objective To evaluate the influence of rectal washout on the prognosis of rectal cancer after anterior resection.Methods Data of 144 patients with rectal cancer undergoing anterior resection at Chinese Academy of Medical Science Cancer Hospital between May 2006 and November 2007 were reviewed.69 patients received rectal washout and 75 patients did not.The incidences of recurrence and 5-year disease-free survival rates were compared.Data were analyzed by SPSS 13.0 software packet,using x2 and t inspection.Kaplan-Meier method was used to calculate postoperative survival rate.Results Washing sample was positive for malignant cells in 55 patients (79.7％).The incidences of recurrence were 4.6％ and 6.7％ in washout group and no-washout group respectively (x2 =0.368,P =0.721).The five-year disease-free survival rate was 79.7％ in washout group and 74.7％ in no-washout group(x2 =0.517,P =0.553).Conclusions Free malignant cells were ubiquitous in rectal lumen during anterior resection,and there was a high positive rate of cytological examine for washout sample.Rectal washout may reduce local recurrence and prolong patients' survival.

Using a UPLC-MS/MS (MRM) and cocktail probe substrates method, the metabolic fingerprint of the compatibility of Radix Aconite (RA) and Radix Paeoniae Alba (RPA) and its effect on CYP450 enzymes were investigated. These main CYP isoforms include CYP 1A2, CYP 2C, CYP 2E1, CYP 2D and CYP 3A. Compared with the inhibition effect of RA decoctions on CYP450 isoforms, their co-decoctions of RA and RPA with different proportions can decrease RA' inhibition on CYP3A, CYP2D, CYP2C and CYP1A2, but can not reduce RA' effect on CYP2E1. The metabolic fingerprints of RA decoction and co-decoctions with different proportions of RPA in CYP450 of rat liver were analyzed by UPLC-MS. Compared with the metabolic fingerprints of RA decoction, the intensity of diester-diterpenoid aconitum alkaloids decreased significantly, while the intensity of monoester-diterpenoid alkaloids significantly increased in the metabolic fingerprints of co-decoctions of RA and RPA. The results suggest that RA coadministration with RPA increased the degradation of toxic alkaloid and show the effect of toxicity reducing and efficacy enhancing.