1.Expression pattern of transcription factor Olig2 in cuprizone-induced mouse model of acute demyelination
Liping CHEN ; Jing ZHANG ; Shunli MA ; Zhenfei LI ; Jinli ZHANG ; Mei DONG ; Mingyue SHAN ; Li GUO
Acta Laboratorium Animalis Scientia Sinica 2014;(2):36-39
Objective To investigate the expression pattern of transcription factor Olig 2 in cuprizone-induced mouse model of acute demyelination .Methods C57BL/6 mice were fed with 0.2%cuprizone to induce acute demyelina-tion.Immunofluorescence and qRT-PCR were used, and Olig2, MBP and GFAP were detected in the brain tissues of con-trol group and cuprizone-treated groups for 6 weeks and recovery for 2 weeks.Results Severe demyelination occurred in the corpus callosum following 6-weeks exposure to cuprizone , while remyelination was detected in the white matter after the mice were given diet without cuprizone .In the normal mice , Olig2 was expressed in a low level , while the experessions of Olig2 and GFAP were significantly increased , and Olig2 +/GFAP+cells were detected after demyelination .But the expres-sion of MBP was below the normal level with demyelination .After recovery for 2 weeks, the experession of Olig2 was lower, but the experessions of MBP and GFAP were increased .Conclusions Olig2 may play an important role in the glial differ-entiation from neural progenitor cells into active astrocytes , and in the glial scar formation .
2.The Killing Effects and Preliminary Mechanism of Natural Plant-derived Antimicrobial Solution(PAMs)to Human Liver Cancer HEPG-2 Cells
Jinyi QIU ; Jiaming HUANG ; Yijie ZHOU ; Rongkun DOU ; Zhenfei BI ; Yalan YANG ; Yinsong DONG ; Danzhou XIANGFEI ; Canquan MAO
Progress in Modern Biomedicine 2017;17(26):5011-5016
Objective:In this study,a series of experiments were conducted to research the mechanism of anticancer and preliminary molecular effects of PAMs on the HEPG-2 cancer cells.Methods:Morphological observation and MTT assay were used to explore the inhibition and killing effect of PAMs acting on HEPG-2.AO/EB staining and Annexin V-FITC/PI staining were employed to observe the apoptosis of HEPG-2 treated with PAMs.The expression level of Foxm1,bcl-2 and others genes in HEPG-2 cells were detected by using qRT-PCR and western blot.Wound healing and transwell experiments determined if PAMs can inhibit the migration of HEPG-2.Results:PAMs can inhibit and kill HEPG-2 cells in time and dose-dependent manners,and the cytotoxic effects were closely related to the cell apoptosis.The mRNA expression of foxm1,bcl-2 and surviving gene were remarkably decreased in HEPG-2 cells after the treatment of PAMs.PAMs decreased the FoXM1 protein expression in HEPG-2 cells,while up-regulating thep53 protein expression.,and it could also inhibit the migration of cancer cells.Conclusions:The possible molecular mechanism for the killing of HEPG-2 cancer cells by PAMs was proposed.By down-regulating the expression of foxm1 and up-regulating the expression of p53,the transcriptional expression of their downstream target genes survivin and bcl-2 was inhibited or reduced,hence enhancing the cancer cell apoptosis.This study provides an important foundation for the development of anti-cancer Chinese folk medicine based on PAMs.
3. Efficacy and mechanism of Xiaobanxia Decoction and categorized formula in preventing and treating delayed chemotherapeutic vomiting
Jiyan ZHAO ; Jinxi LI ; Yimin ZHANG ; Zhenfei DONG ; Junning WANG
International Journal of Traditional Chinese Medicine 2020;42(2):138-143
Objective:
To observe the pharmacodynamic effect of