To observe the germistatic and germicidal effects of origanum volatile oil (OVI) on the dysentery bacteria, the abdominal cavity of mice was infected with Shigella sonne (Sh. sonnei) and Shigella flexneri (Sh. flexneri) F2a. After OVI was given to the mice via gastric lavage, the effects of OVI on the infected mice were observed. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for dysentery bacteria were determined in vitro. The results showed that origanum volatile oil showed obvious protective effect on mice infected with Sh. sonnei and Sh. flexneri F2a and it had germistatic and germicidal effects on dysentry bacteria. We are led to conclude that origanum volatile oil is an effective medicine against the infection of dysentery bacteria.
Anti-Bacterial Agents/*pharmacology ; Dysentery, Bacillary/*microbiology ; Microbial Sensitivity Tests ; Oils, Volatile/*pharmacology ; Shigella flexneri/*drug effects ; Shigella flexneri/isolation & purification ; Shigella sonnei/*drug effects ; Shigella sonnei/isolation & purification

Object To apply the technique of tissue culture to the rapid propagation of Stemona japo-nica (Bl.) Miq. and lay a foundation for the rapid propagation of its improved breeds. Methods The explants used for culture were stem segments with axillary bud of S. japonica. The media were MS basal media containing different plant hormones. Results The results of the study indicated that shoots could be induced successfully in MS medium supplement with 6-BA 3.0 mg/L and IBA 0.2 mg/L, and the better medium for bud multiplication and root inducement were MS+6-BA 3.0 mg/L+IBA 0.3 mg/L and MS+IBA 2.0 mg/L+AgNO 3 0.5 mg/L respectively. After 30 days' culture, the rate of rooting reached over 50%. The survival rate of transplanting was raised obviously by using 20 mg/L carbendazim treatment. Conclusion The primary plant regeneration system of S. japonica has been established, which makes the industrial production possible.

Traditional DNA sequence analysis is based on sequence alignment, while a new DNA visual sequence analysis is proposed in this paper. Based on S. Wolfram's cellular automation theory, the method transfers one-dimensional DNA sequence into two-demensional visual image. Applying this method to SARS DNA sequence analysis, a characteristic of SARS-CoV differing from non-SARS is discovered. Compared with all known coronaviruses' images, It is found that this is a unique characteristic of SARS virus, and it is helpful to clinical identification of SARS.
Algorithms ; SARS Virus ; genetics ; Sequence Analysis, DNA ; methods

This paper proposed a new kind of visualized method of genome. Using cellular automation theory, the visual method transfers one-dimensional RNA sequence into two-demension visual image. Applying this method to SARS RNA sequence analysis, the characteristic of SARS-CoV differing from Non-SARS is discovered. This paper extracts characteristic genome fragment, visualize them, and study them with some pattern recognition method such as PCA and SVM. The result shows that the characteristic of SARS-CoV is classifiable. Some combined methods can use the characteristic more sufficient as an un-routine method.
Genome, Viral ; Image Processing, Computer-Assisted ; methods ; RNA, Viral ; genetics ; SARS Virus ; genetics ; Sequence Analysis, RNA ; methods

To observe the germistatic and germicidal effects of origanum volatile oil (OVI) on the dysentery bacteria, the abdominal cavity of mice was infected with Shigella sonne (Sh. sonnei) and Shigella flexneri (Sh. flexneri) F2a. After OVI was given to the mice via gastric lavage, the effects of OVI on the infected mice were observed. The minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) for dysentery bacteria were determined in vitro. The results showed that origanum volatile oil showed obvious protective effect on mice infected with Sh. sonnei and Sh. flexneri F2a and it had germistatic and germicidal effects on dysentry bacteria. We are led to conclude that origanum volatile oil is an effective medicine against the infection of dysentery bacteria.
Animals ; Anti-Bacterial Agents ; pharmacology ; Dysentery, Bacillary ; microbiology ; Female ; Male ; Mice ; Microbial Sensitivity Tests ; Oils, Volatile ; pharmacology ; Shigella flexneri ; drug effects ; isolation & purification ; Shigella sonnei ; drug effects ; isolation & purification

Objective : To explore an efficient method for the establishment of three-dimensional (3-D) digital models of maxillofacial region including muscle tissue based on CT and MRI images fusion on a personal computer, integration of CT and MRI data, and provide accurate 3D model for biomechanical analysis. Methods : A male volunteer was scanned on maxillofacial region by spiral CT and MRI. Two kinds of data obtained were imported into Mimics 15. In the three sections, namely the transverse, sagittal, coronal sections, two kinds of data were adjusted to the same anatomical layers. The most obvious anatomical points on each layer were selected as registration points. Then, the multi-points registration was implemented for data fusion. Then the bone and facial skin were segmented and 3D reconstructed using CT data, the main facial muscles were segmented and 3D reconstructed using MRI data. Results: The 3D model including 3 pairs of masticatory muscles, 12 pairs of facial expression muscles, facial skin and jaw tissues were established. Conclusion The efficient registration and fusion of CT and MRI datas were accomplished. Moreover, this method can be used for further segmentation and reconstruction of other important structures in craniofacial area, such skin, blood vessel, fat, lymph node and the brain tissues.