With the development of automated quantitative detection of serum hepatitis B surface antigen ( HBsAg) , HBsAg quantification is becoming increasingly important in management of chronic HBV infections.Studies have shown that HBsAg quantification together with HBV DNA load may be used to monitor the natural history of chronic HBV infection as well as to predict the clinical outcome of interferon treatment.However, there is no consensus on the clinical use of HBsAg quantification for evaluating patients′responses to nucleos( t) ide analogues therapy.This paper reviews the up-to-date clinical studies on the clinical significance of HBsAg quantification in management of chronic HBV infection.

Histone acetyltransferases(HATs) faciliate histone acetylation and histone deacetylases(HDACs) serve to remove acetyl groups from histones.The activation and repression of gene expression can be regulated by the acetylation of histone or specific genes.It is certified that acetylation of related genes is down-regulated in diabetic retinopathy,retinal ischemia-reperfusion,degenerative retinopathy,infective retinopathy and retinal tumors,which results in cell apoptosis and retinal dysfunction.So the physiology and pathology of retina have a close relation.The effects of histone acetylation and deacetylases on retinal diseases are still studying because of the complexity and diversity of genetic modification io epigenetic inheritance.This article reviewed the classification of HATs and HDACs and their inhibitors,their effects and function,their relationship to retinopathy,and discuss the protection of their inhibitors to retina.

Objective To observe the clinical effect of early diabetic nephropathy treated with Fufang-Xueshuantong capsule. Methods 90 hospitalized patients from September 2011 to October 2012 with early diabetic nephropathy were recruited in Shangqiu First people's hospital endocrinology of Henan Province. All patients were divided into two groups randomly, with 45 patients in each group. The control group was treated with diabetes education, blood glucose control, blood pressure control, and blood lipid regulation, while the treatment group was treated with Fufang-Xueshantong capsule on the basis of the control group. The change of blood glucose, HbA1c, urine microalbumin, and Ualb/Cr were measured before and 12 weeks after the treatment. Results ① Compared the urine microalbumin, Ualb/Cr of the two groups before and after treatment:the urine microalbumin, Ualb/Cr of the two groups after the treatment [Treatment group was(24.5±9.1)mg/L,(2.4± 1.6)mg/mmol, Control group was(85.9±8.6)mg/L,(5.6±2.1)mg/mmol] were significantly reduced than before the treatment (P<0.01 or 0.05)[Treatment group was (142.5±20.8)mg/L, (27.1±5.3)mg/mmol, Control group was (141.6±21.6)mg/L, (26.3±5.64)mg/mmol], while the effect of treatment group was better than control group(P<0.05). ② Compared the FBG, 2 hBG, HbA1c of the two groups before and after treatment:After treatment the level of FBG, 2 hBG, HbA1c of the two groups [Treatment group was(5.58±1.57)mmol/L,(7.82±2.43)mmol/L, (7.01± 1.22)%, Control group was(5.67 ± 1.46)mmol/L,(7.71 ± 2.59)mmol/L,(6.83 ± 2.31)%]were also reduced than before(P<0.05). [Treatment group was (8.69±1.59)mmol/L,(12.79±2.50)mmol/L,(9.91±1.98)%, Control group was(8.72±1.56)mmol/L, (12.86±2.25)mmol/L,(9.89±2.03)%], but there was no statistically difference between the groups(P>0.05). Conclusion Fufang-Xueshuantong capsule had good effect in treating early diabetic nephropathy, it can also delay the progress of renal damage.

Surgery has remained the mainstay of treatment of rectal cancer but due to high local recurrence rates,adjuvant chemoradiation has become standard practice for advanced rectal cancer.In 1990,postoperative chemoradiation has been established by NCI based on the results of GITSG,NCCTG and intergroup studies.However,the German CAO/ARO/AIO 94 study showed that preoperative chemoradition is better than postoperative in terms of local control and acute toxicity.The 5-year cumulative incidence of local relapse was lower in patients assigned to pre-operative chemoradiation vs postoperative treatment Grade 3 or 4 acute toxic effects occurred in 27% of patients in pre-op group vs 40% in post-op group.The corresponding rates of long-term toxic effects were 14% and 24%,respectively.Preoperative chemoradiation has been the trend of treatment of advanced rectal cancer.

Objective: To study the factors affecting amplified fragment length polymorphism (AFLP) of Carthamus tinctorins L. genome, and to develop and optimize the AFLP reaction system, laying a foundation for Marker-assisted breeding of Carthamus tinctorins L.. Methods: Cetyl-trimethyl-ammonium-bromide (CTAB) method was used to extract the genomic DNA of Carthamus tinctorins L., whose concentration and purity were determined by UV spectrophotometry. The genomic DNA was digested by restricted enzyme and ligated with one-step method or two-step method so as to find out which one is more suitable. Then the products were prepared with different dilution multiples (5, 10, 15, 20, 25 and 30) for pre-amplification and with different concentrations of gradients (10, 25, 50, 75, 100, 150 and 200) for selected amplification. Then they were subjected to PAGE electrophoresis and silver-staining after denaturing at 95°C for 8 min. Results: The AFLP reaction system for Carthamus tinctorins L. genome included the following steps: the genomic DNA was precipitated with alcohol by CTAB method to avoid contamination by any inhibitors; the DNA was completely digested by Mse I and EcoR I at 37°C for 3 hours. Two-step method was superior to one-step method in this AFLP system. The digestion time was 3 hours at 37°C and ligated at 16°C over night, with Buffer 2 (NEB Company) as the specific buffer. Furthermore, the optimal dilution was 25 folds for pre-amplification and 75 folds for selected amplification. In this reaction system, the strips in PAGE electrophoresis were clear and less decomposed. Conclusion: The AFLP reaction system in this experiment is suitable for silver-stained AFLP of Carthamus tinctorins L. analysis.

Objective To clarify the frequency, the routes of transmission from mother to infant, the correlation factors and distribution of genotypes of transfusion transmitted virus (TTV) infection. Methods Nested polymerase chain reaction (n PCR) was performed to detect TTV DNA and genotypes in serums and breast milks from 160 cases of pregnant women. Results TTV DNA in serum and breast milk was detected in 64 (40 0%) and 60 (37 5%) of 160 cases of pregnant women respectively. The positive rates of TTV DNA from HBV markers (+) and normal groups were 50 0%, 43 1% and 13 6%, 22 7% respectively in serums and breast milk. There were significant difference between the two groups ( P

Acupuncture Therapy ; Adult ; Humans ; Male ; Muscular Atrophy, Spinal ; therapy

OBJECTIVETo observe the changes in the adhesive function of vascular endothelial cells (VEC) and rat monocytes induced by lipopolysaccharide (LPS) and co-cultured with smooth muscle cells (SMC) and the intervention effect of paeonol (Pae).

METHODPrimary rat vascular endothelial cells (VECs) and rat vascular smooth muscle cells (VSMCs) were cultured by predigesting and adhering tissue blocks. The VEC-VSMC co-culture model was established by Transwell chamber. LPS was used to induce VEC injury. MTT assay and LDH assay were used to determine the VEC activity. ELISA assay was used to detect IL-1beta and TNF-alpha secreted by the VEC. The immunocytochemistry assay was carried out to detect the expression of ICAM-1. The Rose Bengal Staining was used to test adhesive function between VECs and monocytes.

RESULTThe concentration of LPS-induced VEC injury was 100 microg x L(-1), and the time was 7 h. after the intervention on the above cell model for 24 h, Paeonol (15, 30, 60 micromol x L(-1)) could effectively inhibit LPS-induced VEC injury and VEC injury, significantly enhance the survival rate of LPS-injured VECs, decrease IL-1beta and TNF-alpha secreted by the injured VEC, and reduce the expression of ICAM-1, so as to inhibit the adhesion of LPS-induced VECs and monocytes.

CONCLUSIONPaeonol could inhibit IL-1beta and TNF-alpha expression to protect VECs from being injured by LPS, and reduce ICAM-1 expression to inhibit the adhesion between VECs and monocytes.

Acetophenones ; pharmacology ; Animals ; Cell Adhesion ; drug effects ; Coculture Techniques ; Endothelial Cells ; cytology ; drug effects ; metabolism ; secretion ; Gene Expression Regulation ; drug effects ; Intercellular Adhesion Molecule-1 ; metabolism ; Interleukin-1beta ; metabolism ; Lipopolysaccharides ; pharmacology ; Male ; Monocytes ; cytology ; drug effects ; Myocytes, Smooth Muscle ; cytology ; Rats ; Rats, Sprague-Dawley ; Tumor Necrosis Factor-alpha ; secretion

The β-adrenergic receptor has close relationship with the occurrence and development of tumor.The β-adrenergic receptors were expressed in a variety of tumors.Its activation can stimulate tumor growth by promoting cell proliferation,inhibite apoptosis,promote angiogenesis,and enhance tumor invasion and metastasis.The β-adrenergic receptor blockers can suppress the tumor growth,which may become one of the effective methods of cancer prevention and treatment.

Objective To explore the changes of the survival quality in stomach cancer patients before and after surgery and its influencing factors.Methods The patoents with stomach cancer have been diagnosed in 202 cases,through the QLQ-C30 scale before another opponent live quality investigation,as well as many variables (conditional Logistic regression) method,the life quality of correlation factors of gastric cancer patients were anelyzed.Results Compared with before treatment,after a month QLQ-C30 various quality of life index compared to baseline state basic recovery,the difference was not statistically significant ( P ＞ 0.05 ),and a week after QLQ-C30 various quality of life index and before treatment is statistically significant ( all P ＜ 0.05 ).Conclusion Gastric cancer patients before and after surgery survival influence factors on the quality of the main factors for the population sociology characteristics,social support,male,operation methods and medical expenses,should strengthen the psychological and social function of patients to improve.