Bone marrow stem cells(BMSCs) can differentiate into cardiomyocytes when induced with proper reagent. The cardiomyocytes from BMSCs have cardiomyocyte phenotypes with the expression of cardiac-specific antigen and receptor. BMSCs differentiate into cardiomyocytes in host myocardial microenvironments. Mechanism of the differentiation is unclear and need to be studied further.

Objective To explore inhibitory effects of ginsenoside on the proliferation of human thyroid canc-er SW579 cells in vitro and expression of C -myc and Bcl -2 protein.Methods Human thyroid cancer SW579 cells were cultured according to conventional method.The study was divided into the control group and ginsenoside group (20,40,8ug/mL).Inhibitory role of ginsenoside on proliferation of SW579 cells was detected by MTT assay.Western-blot method was used to determine expression levels of C -myc and Bcl -2 protein in different group.Results The inhibition rates of 20,40,80μg/mL ginsenoside to SW579 thyroid carcinoma cell were 22.35%,51.76% and 68.24% respectively.Which meaned ginsenoside had obvious inhibitory effect compared with the control group(P <0.01),and inhibition increased with the increase of solubility(P <0.01).C -myc and Bcl -2 protein were reduced progressively in place with the increase of ginsenoside concentration by Western -Blot analysis (P <0.01 ).Conclusion Gisenoside may play the inhibitory role on proliferation of human thyroid cancer SW579 cells in vitro, and its mechanism may be related with down -regulation of C -myc and Bcl -2 protein.

Objective To observe inhibitory effects of Ginsenoside on the proliferation cultured stem cells of human rectal carcinoma in vitro.Methods Tumor stem cells of rectal carcinoma in human were cultured in vitro and divided into saline water group,reltitrexed group(3mg/mL),and Ginsenoside(25μg/mL)plus reltitrexed(3mg/mL) group,with dosage of qd and for 2 weeks.Cells growth was observed under the microscope,and immunofluorescence staining was used to detect CD +133 expression of rectal carcinoma cells.MTT colorimetric method was taken to measure inhibitory of Ginsenoside on proliferation of tumor stem cells of rectal carcinoma.Apoptosis of tumor stem cells of rec-tal carcinoma lead by Ginsenoside was observed by DAPI staining.Results Part of cells were growing up like ball and CD +133 immunofluorescence staining was positive.Inhibitory role of Ginsenoside plus reltitrexed on CD +133 cells of rectal carcinoma appeared on the first day,which was bigger than that of retitrexed by MTT method (P =0.03).Com-pared with the retitrexed group,Gisenoside combined with reltitrexed reduced CD +133 cells expression of rectal carcino-ma,in which optical density and area density of CD +133 cells were obviously decreased with statistical significance (P =0.007,P =0.006,respectively).Conclusion Gisenoside plays the remarkably inhibitory role on proliferation of CD +133 cells of rectal carcinoma.

Objective To analyze the influencing factors of epilepsy in patients with glioma,and to explore the relationship between the epileptic factors and the recurrence of epilepsy after surgery.Methods From January 2012 to December 2015,clinical data of 95 glioma patients in the Fifth Affiliated Hospital of Zhengzhou University were retrospectively analyzed,which were divided into two groups,including 54 cases with epilepsy and 41 cases without epilepsy,all patients were underwent tumor resection,to search and resect epilepsy lesions in patients with epilepsy,Follow-up using outpatient examination and telephone interview was performed to record seizure recurrence after operation,sort and analyze the clinical data of patients,to investigate the influencing factors of epileptic seizures,and the relationship between the factors associated with epileptic seizures and postoperative recurrence of epilepsy.The epileptogenic factors were analyzed using x2 test and multifactor Logistic regression model,epileptogenic factors and recurrent epilepsy after operation were analyzed usingx2 test.Results Logistic regression model showed that peritumoral edema ＞ 2 cm(OR =6.905,95％ CI:2.787-17.106),low tumor pathological grade (0R =5.032,95％ CI:1.696-14.931) were independent risk factors for epileptic seizures;Follow-up situations:16 cases (13 cases of epilepsy group,3 cases of no epilepsy group) showed epileptic seizure in different time after the operation,preoperative epilepsy history,peritumoral edema were related to the recurrence of epileptic seizures in epilepsy group and the patients without epilepsy (x2 =2.940,3.049,all P ＜ 0.05),the pathological grade was related to the recurrence of epileptic seizures in epilepsy group (x2=4.774,P ＜ 0.05),however,it was not associated with the patients without epilepsy (x2 =0.060,P ＞ 0.05),and there was a correlation between the preoperative history of epilepsy and the postoperative recurrent seizures (x2 =4.672,P ＜ 0.05).Conclusions Peritumoral edema ＞ 2 cm and low tumor pathological grade are the factors that lead to epileptic seizures and postoperation epilepsy recurrence in glioma patients.It's expected to take some positive treatment to reduce epileptic seizures,we should pay special attention to glioma patients with epilepsy.

Objective To investigate the feasibility of pig lung cancer model induced by bronchial perfusion of 3,4-benzopyrene. Methods 24 experimental pig were randomly divided into model group and control group, each containing 12 cases. Experimental pigs were under the anaesthetic state, pigs in the model group were given endobronchial infusion of 3,4 - benzopyrene - corn oil mixture, pigs in control group were injected with equal capacity of corn oil.Perfusion 1 times a week for 16 weeks.In week 16,32 and 48,all experimental pig were given lung CT scans, then the lung lesions were observed.After 48 weeks, the pig lung, esophagus, and gastrointestinal tract, liver and brain and other organs were dissected, the presence of tumor formation was observed, and the mass, and experimental pig lung biopsy were given hematoxylin and eosin staining analysis. Results Lung cancers were not found in control group by both CT lung cancers and anatomy. In the model group, pulmonary CT showed space-occupying lesions with different location and size in lungs of 8 pigs, and the space-occupying lesions were confirmed as malignant tumors by pathology, including 3 cases of moderately differentiated adenocarcinoma, 2 cases of highly differentiated squamous cell carcinoma, 1 case of alveolar cell carcinoma,2 cases of adenosquamous carcinoma. Three other pigs and pigs in the control group were not found with tumor by both lung CT and anatomy. Pigs in model group were induced successfully to malignant tumor in 1 year , the total tumor formation rate was 75%,lung tumor formation rate was 66.66%. Conclusion The trachea bronchial perfusion of 3,4- benzopyrene is a simple,safe and reliable way to construct animal models of lung cancer.

Objective] Discussing the relationship between COPD phenotypes and patterns of TCM. [Methods] Discussing the relationship by searching of documents about research of COPD phenotypes. [Result]The phenotype of chronicbronchitis and exacerbation, emphysema,inflammation,FEV1 declining or overlap syndrome may match with different patterns of TCM.[Conclusion] COPD phenotypes have similarities with patterns of TCM at component, influence and typing target.

Chronic Disease ; Constipation ; surgery ; Humans

Air Pollutants ; analysis ; Dust ; analysis ; Environmental Monitoring ; instrumentation ; methods ; Humans ; Inhalation Exposure ; analysis ; Respiration

Objective To construct the plasmid that can produce long,double-stranded RNA and identify nonspecific apoptosis induced by long, double-stranded RNA in human osteosarcoma cells. Methods Plasmid pCI-neo-dsRNA was constructed by two promoters driven DNA sequence based on nontoxic, completely exoteric gene EGFP from the head and the end simultaneously to synthesize sense and antisense RNAs. Human osteosarcoma cells MG63 were transfected with pCI-neo-dsRNA and 48 h later the transfected cells were assessed by Apoptosis Detection System, FCMand MTT method. Results Plasmid pCI-neo-dsRNA was constructed successfully, MTT measurement showed that the absorbance of cells decreased gradually with the increase of plasmid pCI-neo-dsRNA dose(0.05 ?g, 0.10 ?g, 0.15 ?g, 0.20 ?g,0.25 ?g,0.30 ?g). MG3 transfected with pCI-neo-dsEGFP presented a great amount of irregular green fluorescence that indicated cell apoptosis. As the quantity of dsRNA increased, the percent of apoptosis cells also raised. While the dose of pCI-neo-dsEGF was 3.0 ?g, the apoptotic results of pCI-neo-dsEGF in MG3 were similar to that of 5-FU. Conclusion Long, double- stranded RNA could trigger human osteosarcoma cell apoptosis and the further research on using long, double-stranded RNA in osteosarcoma gene therapy can be done based on this theory.

[Objective]To observe the bone repairing efficacy of large segmental femur defects in rabbits with calcium phosphate cement(CPC)combined with bone morphogenetic protein(BMP)and massive bone allograft,which may benefit the clinical application of large segmental bone allograft transplantation.[Method]Fifty-four New Zealand white rabbits were divided into three groups and a 2 cm femur defect was created on one side of each rabbit,followed by implantation with:CPC combined with BMP and massive bone allograft(Group A),bone allograft only(Group B)and autograft transplantation(Group C).Intramedullary nails with a 3 mm diameter fixed all the grafts transplanted.The bone defect repair efficacy was evaluated by radiology and histology exam at 4,8 and 12 weeks after operation.[Result]The bone reparation capacities of allograft with CPC/BMP complex was better than that of the allograft alone after 4 ~8 weeks of transplantation,which were similar to the result of autograft transplantation.Complete bone union was achieved for all the groups after 12 weeks of operation,with better bone remodeling for group A and group C transplantation.The healing process of CPC/BMP combined with allograft transplantation was featured with large amount of bone callus forming surrounding the graft-host bone union area and the surface of allograft,which composd the extra cortical bone bridge and ingrowth(EBBI).Bone invasion,resorption as well as new bone genesis were seen in the surface of bone allograft at early stage,companied by expansion of Haversians canal,with more lanner cells,osteoblasts,osteoclasts and blood cells inside the allograft.CPC was slowly biodegraded with the bone graft resorption and new bone regeneration.[Conclusion]CPC combined with BMP can improve the bone reparation and substitution process in massive bone allograft transplantation for the treatment of large segmental bone defects.