To explore the related risk factors and outcome in pregnancy- induced hypertension patients with Ⅲ phase of retinopathy.
●METHODS: A total of 105 pregnancy - induced hypertension patients with Ⅲ phase of retinopathy in our hospital from Januany 2012 to December 2013 were enrolled. Clinical date of them were collected to analyze.
●RESULTS: The occurrence of pregnancy - induced hypertension patients with Ⅲ phase of retinopathy were positively correlated with the course of the disease, blood pressure, proteinuria, and it was higher occurred in cold winter and spring, timely termination of pregnancy and appropriate hormone therapy can promote the recovery of vision, and improve outcomes of pregnancy.
●CONCLUSlON: The occurrence of pregnancy - induced hypertension patients with Ⅲ phase of retinopathy associated with season and disease severity. Timely treatment can restore normal vision, improve maternal and neonatal prognosis. Routine examination of fundus examination should be used as the pregnancy induced hypertension syndrome.

OBJECTIVETo explore the correlativity of meridians and acupoints with fascial system.

METHODSThe lines and beads-like structures seen by fasciaology and scanning connective tissue, were combined with traditional meridians and collaterals, and acupoints to investigate channels, collaterals and acupoints.

RESULTSThe high correlativity of the meridians and acupoints with the fascial system was found.

CONCLUSIONThe concept, functions, clinical application and mechanisms of meridians and acupoints can be preliminarily explained.

Acupuncture Points ; Computer Simulation ; Fascia ; physiology ; Humans ; Medicine, Chinese Traditional ; Meridians

BACKGROUNDThe medial plica may be caused by direct trauma or joint degeneration, which also could be iatrogenic. There have been few reports in the literature discussing incidence of the medial plica caused by an operation on the knee joint, specifically after the reconstruction of anterior cruciate ligament (ACL). In this study, we aimed to evaluate and analyze the relationship between the incidence of the medial plica and reconstruction of the ACL.

METHODSA retrospective case series study was conducted to review the findings of 1085 patients between 2003 and 2007, who underwent second-look arthroscopy after reconstruction of the ACL (between 2002 and 2006). The correlation of the incidence of medial plica with the stability of the knee joint, the time from onset of injury to reconstruction surgery, the associated injuries, and the rate of progress during postoperative rehabilitation were analyzed.

RESULTSWe found that 722 patients had the structure of a medial plica. The incidence after reconstruction of the anterior cruciate ligament (66.5%) was significantly higher than usually reported. All these medial plica had avascular fibrotic and thickened edges. An excision of pathologic medial plica and fat pad synovial fringes were done. The incidences were significantly different between the two groups with their reconstruction operation time, from onset of injury to surgery (less than one month or over 2 years), and the progress rate of postoperative rehabilitation (knee flexion could not be over 90 degrees in four weeks). The incidence was not different between the groups with knee stable conditions.

CONCLUSIONSMedial plica is more common in patients after reconstruction of ACL. More associated injuries and more rehabilitation difficulties can increase the medial plica incidence.

Adolescent ; Adult ; Anterior Cruciate Ligament ; surgery ; Female ; Humans ; Joint Diseases ; etiology ; pathology ; Knee Joint ; pathology ; surgery ; Male ; Middle Aged ; Patella ; pathology ; Reconstructive Surgical Procedures ; methods ; Retrospective Studies ; Tendon Transfer ; methods ; Treatment Outcome ; Young Adult

OBJECTIVETo observe the effect of Chinese drugs for strengthening Pi, harmonizing Wei, and dispersing blood stasis (CDSPHWDBS) on the expression of gastric mucosal heat shock protein 70 (HSP70) in chronic atrophic gastritis (CAG) patients.

METHODSA total of 100 CAG patients were assigned to the control group and the treatment group by random digit table, 50 in each group. Patients in the control group took Folic Acid Tablet 10 mg each time, 3 times per day. Those in the treatment group took CDSPHWDBS, 100 mL each time, once per day. The treatment course was 6 months for all. Clinical symptoms and signs, endoscopic and histopathological changes were observed before and after treatment in the two groups. The expression of gastric mucosal HSP70 in CAG patients was determined using SP immunohistochemistry. Data were collected by HPIAS-1 000 pathological graphic analysis system, and its expression semi-quantitatively analyzed.

RESULTSThe total effective rate of clinical Chinese medical symptoms and signs was 88. 0% (44/50 cases) in the treatment group and 56. 0% (28/50 cases) in the control group, with significant difference between the two groups (P <0. 01). The improvement rate of endoscopic manifestations such as congestion and edema, erosion, bile regurgitation, pale gastric mucosa, exposed blood vessels, particles proliferation in the treatment group were superior to those in the control group (P <0. 05). The total effective rate of atrophy was 80. 0% (40/50 cases) in the treatment group and 54. 0% (27/50 cases) in the control group, with significant difference between the two groups (P<0. 01). The effective rate of intestinal metaplasia was 75. 0% (12/16 cases) in the treatment group and 33.3% (5/15 cases) in the control group, with significant difference between the two groups (P < 0. 05). The optical density value of gastric mucosal HSP70 was significantly elevated in the two groups after treatment (both P <0. 05). It was higher in the treatment group than in the control group after treatment with significant difference (P <0. 01).

CONCLUSIONCDSPHWDBS had obvious effect in treatment of CAG and could improve pathological changes of precancerous lesions possibly by promoting the expression of gastric mucosal HSP70 in CAG patients.

Drugs, Chinese Herbal ; therapeutic use ; Gastric Mucosa ; metabolism ; Gastritis, Atrophic ; drug therapy ; metabolism ; HSP70 Heat-Shock Proteins ; metabolism ; Humans ; Immunohistochemistry ; Medicine, East Asian Traditional

OBJECTIVETo explore the method for obtaining olfactory ensheathing cells from human fetal olfactory mucosa by cell culture for selective adhesion in the presence of neurotrophin-3 (NT3) and low-concentration serum.

METHODSThe olfactory ensheathing cells were cultured alternatively in DMEM/F12 culture medium containing 10% fetal bovine serum (FBS) and the medium containing NT3 and 2.5% FBS every 72 h. The cells were observed for morphological changes and identified using immunocytochemistry with P75NTR and GFAP, and the cell purity was estimated.

RESULTSThe olfactory ensheathing cells from human fetal olfactory mucosa were positive for P75(NTR) and GFAP, and in in vitro culture, the cells exhibited dipolar or tripolar appearance with long thin neurites. On the 9th day of cell culture, the purity of the olfactory ensheathing cells reached about 83%.

CONCLUSIONThe olfactory ensheathing cells can be obtained by in vitro culture for selective adhesion in the presence of NT3 and low-concentration serum.

Cell Culture Techniques ; methods ; Cell Separation ; methods ; Cells, Cultured ; Culture Media ; Fetus ; Humans ; Neurotrophin 3 ; pharmacology ; Olfactory Bulb ; cytology ; Olfactory Mucosa ; cytology

OBJECTIVETo develop an HPLC method for determination of two polyacetylenes, lobetyolin and lobetyolinin, in Herba Lobeliae Chinensis.

METHODC18 column was used with the mobile phase consisted of acetonitrile and water. Linear gradient elution from 10% to 40% acetonitrile in 25 min was applied, at the flow rate of 1.0 mL x min(-1), the detection wavelength was at 267 nm.

RESULTLower contents of lobetyolin and lobtyolinin were found in collected samples of Herba Lobeliae Chinensis. The highest amounts of lobetyolin and lobetyolinin were found to be 0.461 and 0.436 mg x g(-1) in a sample procured from Hong Kong. However, there were no lobetyolin and lobetyolinin in some of the samples.

CONCLUSIONA simple and effective HPLC method to analyze the two polyacetylenes in Herba Lobeliae Chinensis was established. It could be applied for the quality control of this herb.

Chromatography, High Pressure Liquid ; methods ; Lobelia ; chemistry ; Molecular Structure ; Plants, Medicinal ; chemistry ; Polyacetylenes ; analysis ; chemistry ; Quality Control ; Reproducibility of Results

BACKGROUNDPectoralis major tendon rupture is a relatively rare injury leading to both functional and cosmetic deficiency. The peak torque of the pectoralis major is significantly decreased after conservational treatment of a total rupture. We suppose that surgical intervention is better choice.

METHODSWe retrospectively evaluated 12 patients who were diagnosed with distal pectoralis major muscle rupture and underwent operation in our institute from 1993 to 2007. All patients were male with a mean age of 32 (19 - 54) years. In 4 patients, tendon reconstruction was accomplished by fixing the tendon to the humerus using sutures passed through predrilled humeral bone tunnels. In 8 patients, tendon reconstruction was accomplished by directly suturing the muscle to the tendon. All patients followed an accelerated rehabilitation protocol. Treatment results were evaluated according to the following criteria: (1) visual analogue pain scale, (2) isokinetic strength measurements, (3) range of motion of shoulder joint, (4) cosmetic result, and (5) postoperative sports activity performance. In this study, we aimed to describe our surgical technique of reconstruction of the rupture of pectoralis major muscle and to summarize the clinical results of the operative treatment.

RESULTSAt the final follow-up examination (6.5 years postoperatively), only nine patients were available for evaluation. Three of them had excellent results, and five had good results, while one had poor results. Eight of the patients were able to return to their preinjury level of sporting activity. In summary, 89% of the patients achieved excellent or good results.

CONCLUSIONSurgical treatment by anatomic tendon repair and accelerated rehabilitation can make recovery of strength and function of the pectoralis major muscle.

Humans ; Male ; Middle Aged ; Pectoralis Muscles ; injuries ; surgery ; Rupture ; surgery ; Tendon Injuries ; surgery ; Treatment Outcome ; Young Adult

Adult ; China ; Female ; Humans ; Influenza Vaccines ; Logistic Models ; Male ; Middle Aged ; Surveys and Questionnaires ; Vaccination ; psychology ; statistics & numerical data

Rsm (repressor of secondary metabolite) A is an mRNA binding protein which functions as a global repressor to control multiple genes at the posttranscriptional level. Using homologous recombination technique a chromosomal rsmA inactivated mutant strain M-18R was constructed in Pseudomonas sp. M-18, a strain of plant-growth-promoting rhizobacteria, which could inhibit several soilborn phytopathogens by producing secondary metabolites including phenazine-1-carboxylic acid (PCA) and pyoluteorin (Plt) in one single strain. To further study the effect of RsmA on the synthesis of Plt and PCA in the wild type strain M-18, the dynamic curves of Plt and PCA produced respectively by M-18 and M-18R were measured in KMB medium under different temperature conditions such as 37 degrees C constant, 28 degrees C constant and nonconstant (37 degrees C 4 hours at first and then 28 degrees C constant) cultivation. The synthesis of both Plt and PCA were almost inhibited in the cultures under the condition of 37 degrees C. At 28 degrees C, however, compared with the wild type strain M-18, the mutant strain produced tenfold amount of Plt, while the production of PCA decreased only about 50%. When cultivated under the nonconstant condition, the amount of Plt produced by M-18R could reach 400 microg/mL while the PCA production was not significantly affected, but in the wild type strain M-18, the amount of Plt production decreased obviously while the PCA production was not affected in comparison with the results at 28 degrees C constant. These results suggest that a temperature sensitive factor exists to function as an activator independent of RsmA to promote the synthesis of Plt in the rsmA mutant strain M-18R while it may bind with RsmA to repress the synthesis of Plt in the wild type strain M-18. But this factor did not exert any affect on the synthesis of PCA.
Bacterial Proteins ; genetics ; metabolism ; Mutation ; Phenazines ; metabolism ; Phenols ; metabolism ; Pseudomonas ; genetics ; growth & development ; metabolism ; Pyrroles ; metabolism ; Repressor Proteins ; genetics ; metabolism ; Temperature

Objective:To investigate the clinical features of patients undergoing simultaneously defunctioning stoma for sphincter saving surgery for low and middle rectal cancer in our center, and to analyze the influencing factors of physicians′ choice of defunctioning stoma, which is expected to be used to guide clinicians′ preoperative prediction and decisions.Methods:Patients who underwent laparoscopic assisted sphincter saving surgery for low and middle rectal cancer between Jan. 2015 and Dec. 2020 in the Department of Gastrointestinal and Anal Surgery, the First Hospital of Jilin University were surveyed. The clinical data for each patient were collected and analyzed. Statistical software SPSS24.0 was used for statistical analysis. Factors which could influence the choice of defunctioning stoma probably were conducted by univariate analysis and Logistic analysis.Results:Six hundred and twenty-seven patients were inciuded in this study. There were 376 males and 251 females, median age was 62 years old (aged from 23 to 89). And 285 patients underwent simultaneously defunctioning stoma and 342 patients did not.Patients with middle rectal cancer were 495 and with low rectal cancer were 132. The factors influencing the choice of defunctioning stoma were selected in univariate analysis: male( P<0.001), BMI≥28 kg/m 2( P<0.001), diabetes( P<0.001), neoadjuvant chemoradiation( P<0.001), taTME or ISR operation( P<0.001), anastomosis height <5 cm( P<0.001) and length of surgery more than 180 min ( P<0.001), all of which were statistically significant ( P<0.05). Logistic analysis showed the factors of defunctioning stoma were as follows: BMI≥28 kg/m 2( OR=2.541, 95% CI: 1.079~5.977, P=0.033), diabetes( OR=4.216, 95% CI: 2.258~7.873, P<0.001), neoadjuvant chemoradiation ( OR=4.254, 95% CI: 1.193~15.170, P=0.026), taTME or ISR operation( OR=3.222, 95% CI: 1.678~6.185, P<0.001), anastomosis height <5 cm( OR=10.475, 95% CI: 6.663~16.466, P<0.001) and length of surgery more than 180 min( OR=2.881, 95% CI: 1.713~4.845, P<0.001)( P<0.05). Conclusions:According to single-center experience, physicians' choice of simultaneously defunctioning stoma during sphincter saving surgery for low rectal cancer in abdominal endoscopy is related to many factors, among which diabetes, preoperative neoadjuvant chemoradiation, taTME or ISR operation, anastomosis height <5 cm, long operation time, physicians prefer to choose simultaneously defunctioning stoma. For patients with the above features, they should be adequately prepared preoperatively.