Pancreatic cystic lesions are being recognized increasingly due to the development of computed tomography and magnetic resonance imaging.Differential diagnosis between pseudocysts and cystic tumors is the key point for pancreatic cystic lesions.Patients with pancreatic pseudocysts generally have a history of acute or chronic pancreatitis,whereas patients with cystic tumors did not have such a history.Some pancreatic cystic lesions did not have typical imaging presentations,and they were difficult to be diagnosed.Pancreatic cystic lesions could be divided into unilocular cysts,microcystic lesions,macrocystic lesions and cysts with a solid component according to the presentations under computed tomography and magnetic resonance imaging.This typing system is helpful for the diagnosis and differential diagnosis of pancreatic cystic tumors,as well as for the guidance of therapy.

Objective To evaluate the effectivness of photodynamic therapy (PDT) with chloroaluminum sulfonated phthalocyanine (CASPc) in B16F10 melanomas in a rabbit model. Methods B16F10 murine melanoma tumor fragments were implanted transclerally into the subchoroidal space of 38 immunosuppressed New Zealand albino rabbits and examined with indirect ophthalmoscopy and ultrasonography. When the tumors ranged from 2.0~3.8 mm in height, 30 rabbits were treated by PDT, with intravenous injection of CASPc 5 mg/kg, and irradiation at the wavelength of 675 nm of an argon-pumped dye laser after 24 hours. Light dose ranged from 20 J/cm 2 to 70 J/cm 2. The other 8 animals were treated with light only or photosensitizer only. The animals were followed up for 6~8 weeks. Results The 30 tumor-bearing rabbits were by PDT (laser and CASPc) regressed in 21 animals after treatment. At light doses under 40 J/cm 2, tumor regrowth was observed in 9 animals after two weeks of treatment. In all of the 8 control animals, the tumor-bearing eyes were filled with tumor at the third week after implantation with laser doses of 70 J/cm 2. Conclusion The study suggest that CASPc PDT may be effective in the treatment of B16F10 choroidal melanomas.

Objective To explore the kinds of Traditional Chinese Medicine(TCM)syndromes of depression to establish the criteria of common TCM syndromes of depression. Methods According to the ways of clinical epidemiology, TCM Bianzheng and syndromes’ indexes were investigated in 1977 depression patients with unipolar and bipolar affective disorder in 8 investigation sites such as Hunan, Tianjin, Beijing, Shenzhen, Guanxi, Hujian and Guizhuo. Results There were 12 kinds of syndromes in depression. According to order of composition ratio, the first four syndroms respectively were Gan-Yu-Qi-Zhi, Gan-Yu-Pi-Xi, Gan-Yu-Tan-Zu, Xin-Pi-Liang-Xu, the ratios of which were 29 7%, 24 5%, 13 4% and 12 8% respectively.Conclusions The common TCM syndromes of depression are such as the followings: Gan-Yu-Qi-Zhi, Gan-Yu-Pi-Xi, Gan-Yu-Tan-Zu, Xin-Pi-Liang-Xu. There are some relationships between common TCM syndromes of depression and 4 sub-types of unipolar disorder.

Objective:To study the effect of edaravone on EAE rats and the underlying mechanism.Methods:Wistar rats were immunized with GPSCH,and randomly divided into control group,EAE group,dexamethasone group,low dose of edaravone group and high dose of edaravone group.The morbidity of disease and clinical signs were observed.The pathological changes of spinal cord tissue sections were observed under light microscopy after HE staining and trichrome staining.The expression of Nrf2 and HO-1 was observed by immunohistochemistry.Results:Morbidity of high dose of edaravone group (8.33%) and DXM group (0%) was significantly lower than in EAE group (58.3%) (P

Hallermann's Syndrome ; Humans ; Infant ; Male

Objective To observe the pregnancy outcome of perinatal in drugs abuse pregnant women.Methods The clinical data of 105 cases of newborn in pregnant women with drug abuse were analyzed retrospectively,including premature,neonatal asphyxia,low birth weight,neonatal respiratory distress syndrome (NRDS),intracranial hemorrhage,neonatal congenital malformation and death.Fifty cases of newborn of healthy pregnant women were selected as control group.The occurrence of neonatal withdrawal syndrome was observed.Results There were 80 cases of natural labor,25 cases of cesarean section in 105 cases of pregnant women with drug abuse.Among them,56 cases (53.3 ％) of premature delivery,the average birth weight was(2 534 ± 1 234) g,25 cases(23.8％) of neonatal asphyxia,18 cases(17.1％) of neonatal respiratory distress syndrome (NRDS),16 cases (15.2 ％) of intracranial hemorrhage,and 3 cases (2.9 ％) of congenital malformation.The gestational age and weight of newborn with maternal drug abusing were significantly lower than those of the control group,the incidence of premature infants,low birth weight infants,neonatal asphyxia,NRDS and intracranial hemorrhage were higher than those of the control group,and the differences were significant (P ＜ 0.05).Compared with drug abuse less than 2 years,the proportion of preterm infants,low birth weight,neonatal asphyxia and NRDS were higher than those of drug abuse more than 2 years(P ＜0.05).The proportion of preterm infants,low birth weight,neonatal asphyxia and NRDS of intravenous drug users were higher than those of oral drug users,the differences were significant (P ＜ 0.05).The red blood cellcount,aspartate amino transferase,white blood cellcount and alanine aminotransferase in the drug abusing group were higher than those in the control group,platelet and albumin were lower than those in the control group (P ＜0.05).A total of 30 neonates had the manifestations of neonatal withdrawal syndrome.In 105 cases of newborns,99 cases cured,6 cases died,the cure rate was 94.3％.The causes of death included 3 cases of NRDS complicated with pulmonary infection,1 case of severe intracranial hemorrhage,1 case of asphyxia,and 1 case of multiple organ failure.Conclusion Drug abuse can lead to the increase of premature birth rate,asphyxiation and NRDS.Late pregnant women with drug abuse can cause the incidence of neonatal withdrawal syndrome.

This article was aimed to explore the intelligent integration of database as the core-data and application of data mining as the core of knowledge discovery. On the basis of the existed set of information-sharing platform, this article elaborated from aspects of formatting HER intelligent integration, data integration and multi-method combined data mining intelligent integration. With the demonstration study on syndrome distribution and syndrome diagnostic criteria of acute exacerbations of chronic obstructive pulmonary disease (AECOPD), data integration and intelligence optimization of Crystal Reports and complex networks were applied to dig out the correlation between syndromes and symptoms, disease-syndromes-herb association analysis. Analysis mentioned above showed that based on the shared system, and in accordance to research programs, intelligent integration of data and data mining methods, the improvement of the sharing platform application is proved feasible.

0.05). The 5-year survival rates were 77.4% in LS group and 75.7% in OS group for stage Ⅰ/Ⅱ (P=0.626), and 71.8% in LS group and 65.6% in OS group for stage Ⅲ(P=0.517), respectively. Conclusion: Long-term effect of laparoscopic resection is similar to that of open resection for colon cancer, but laparoscopic surgery has less long-term complications.

Objective Establish rabbit VX2 soft tissue tumor model,and treat it with percutaneous ethanol injection(PEI)under the guidance of magnetic resonance imaging.Make ready for the therapeutic evaluation with functional magnetic resonance imaging. Methods Fifteen healthy New Zealand white rabbits were included in this study.0.2 mL tumor tissue suspensions were injected into the rabbits’posterior limb.14 days later,all rabbits were underwent conventional MRI examination.PET were performed to all the tumors under the guidance of MRI in the next day of the examination.T2 WI was used as guidance and monitoring means.MR com-patible puncture needle with lateral hole was stabed into the lesion center,and inj ected anhydrous ethanol according to the volume of tumors’diameter (1 mL/cm )slowly.the tumors signal characteristics,morphological feature and pathological feature were ob-served pre and post-operation.Results All of the 1 5 rabbits were established soft tissue tumor model successfully;the success rate is 100%.The tumors were oval or round,3-4 cm in diam.MRI scanning showed low signal on T1 WI and high signal on T2 WI be-fore PEI.PEI was performed to all the tumors under the guidance of MR successfully with 3.5 mL ethanol injected into the tumors in average.T2 WI could monitor the ethanol in dispersion and distribution within the tumors clearly.Histologically,tumors were composed of large,uniform,oval/round cells arranged in solid nests which was intensive in the periphery of tumors.Necrosis tissue was apparent in the center of the tumors.10 days after operation,most tissue in the periphery of tumors was coagulative necrosis , only a few tumor cells left.Ranges of necrosis in the tumors center were obviously increased compared with pre-operation.Conclusion Rabbit VX2 tumor of soft tissue model is suitable for the therapeutic evaluation of tumor .It is an animal model which has the characteristic of simple to operate and high rate of suc-cessful.MR T2 WI can monitor the ethanol in dispersion and distribution within the tumors clearly.It is a good guidance and monitoring imaging method of tumor ablation.

Purpose To prepare streptavidin-tagged mouse interleukin-4(mIL-4-SA)bifunctional fusion protein and to study on its bioactivity.Methods The mIL-4 gene was cloned by RT-PCR and cloned into pET21 vector to get mIL-4-SA-pET21 expression plasmid.The mIL-4-SA fusion protein was expressed in BL21 (DE3)host bacteria and purified through the Ni-NTA affinity chromatography and refolded by dilution and dialysis.The effect of mIL-4-SA fusion protein on mouse thymocytes proliferation was evaluated by MTY.Flow cytometric analysis was performed to detect the mIL-4-SA fusion protein on the biotinylated B16F10 tumor cells.Results The mIL-4-SA-pET21 vector was successful by constructed and the mIL-4-SA fusion protein was expressed in BL21(DE3)at about 35%of total bacterial proteins.The purity of mIL-4-SA Was about 95% through Ni-NTA.The mIL-4-SA fusion protein exhibited bifunctional activities,i.e.,stimulative effect for mouse thymocyte proliferation and SA-mediated high-affinity binding to biotinylated cell surfaces(anchoring modified rate Was about 96.69%).Conclusion The mIL-4-SA fusion protein was expected to be developed for the treatment of tumors.