1.A report of 4 cases with tracheal bronchus.
Yue-jie ZHENG ; Dao-zhen ZHANG ; Ji-kui DENG
Chinese Journal of Pediatrics 2006;44(9):698-699
Bronchi
;
abnormalities
;
pathology
;
Bronchial Diseases
;
complications
;
congenital
;
diagnosis
;
pathology
;
Bronchoscopy
;
Child, Preschool
;
Diagnosis, Differential
;
Female
;
Humans
;
Infant
;
Male
;
Pneumonia
;
etiology
;
physiopathology
;
Respiratory System Abnormalities
;
complications
;
diagnosis
;
pathology
;
Trachea
;
abnormalities
;
pathology
;
Tracheal Stenosis
;
etiology
;
pathology
2.A convenient and stereoselective synthesis of (Z)-allyl selenides via Sm/TMSCl system-promoted coupling of Baylis-Hillman adducts with diselenides.
Yun-kui LIU ; Dan-qian XU ; Zhen-yuan XU ; Yong-min ZHANG
Journal of Zhejiang University. Science. B 2006;7(5):393-396
A simple and convenient procedure for stereoselective synthesis of (Z)-allyl selenides has been developed by a one-pot reaction of diselenides with Baylis-Hillman adducts in the presence of samarium metal-trimethylsilyl chloride under mild conditions. Presumably, the diselenides are cleaved by Sm/TMSCl system to form selenide anions, which then undergo S(N)2' substitution of Baylis-Hillman adducts to produce the (Z)-allyl selenides.
Magnetic Resonance Spectroscopy
;
Molecular Structure
;
Organoselenium Compounds
;
chemistry
;
Selenium Compounds
;
chemical synthesis
;
chemistry
;
Stereoisomerism
3.Expression and Antigenic Characterization of the Epitope-G1 of the Bovine Ephemeral Fever Virus Glycoprotein in Pichia pastoris
Fu-ying, ZHENG ; Guo-zhen, LIN ; Chang-qing, QIU ; Kui-zhang, YUAN ; Jun-ying, SONG
Virologica Sinica 2007;22(5):347-352
The epitope-G1 gene of Bovine ephemeral fever virus (BEFV) glycoprotein was synthesised by PCR and cloned into expression vector pPIC9K to construct recombinant plasmid pPIC9K-G1. Then the pPIC9K-G1 was linearized and transformed into Pichia pastoris GS 115. The recombinant P. pastoris strains were selected by a G418 transformation screen and confirmed by PCR. After being induced with methanol, an expressed protein with 26 kDa molecular weight was obtained, which was much bigger than the predicted size (15.54 kDa). Deglycosylation analysis indicated the recombinant G1 was glycosylated. Western blot and ELISA tests, as well as rabbit immunization and specificity experiments indicated that the target protein had both higher reaction activity and higher immunocompetence and specificity. The recombinant G1 protein could be used as a coating antigen to develop an ELISA kit for bovine ephemeral fever diagnosis.
4.Establish Assessment Model of 18 Years of Age in Chinese Han Population by Mandibular Third Molar.
Fei FAN ; Xin-hua DAI ; Liang WANG ; Yuan LI ; Kui ZHANG ; Zhen-hua DENG
Journal of Forensic Medicine 2016;32(1):31-44
OBJECTIVE:
To explore the value of estimating chronologic age based on the grades of mandibular third molar development. To evaluate whether mandibular third molar could be used as an indicator for estimating the age under or over 18 years.
METHODS:
The mineralization status of mandibular third molar of 1 845 individuals aged 10 - 30 was graded and marked based on Demirjian's classification of grades reformed by Orhan. Gender difference was examined by t-test. A cubic regression model was established to analyze the correlation between third molar and chronologic age. Each grade of age cumulative distribution diagram and ROC curve was respectively performed to evaluate the relationship between third molar and the age of 18. Using Bayes discriminant analysis, an equation was established for estimating the age of 18.
RESULTS:
The inner-rater reliability was 0.903. Statistical analysis showed a moderate correlation between age and grade. Significant differences of both genders were found only in grade D and H (P < 0.05). Males at the grades from 1 to D and females at the grades from 1 to C were under 18 years old, and both males and females at grade H were over 18 years old. The area under the ROC curve was 0.797 (P < 0.05).
CONCLUSION
Third molar development shows a high correlation with age, and combined with other indicators, it can be used to estimate the age of 18.
Age Determination by Teeth/methods*
;
Asian People
;
Bayes Theorem
;
China
;
Female
;
Forensic Dentistry
;
Humans
;
Male
;
Molar, Third/diagnostic imaging*
;
Radiography, Panoramic
;
Reproducibility of Results
;
Sex Characteristics
5.Relation between Body Height and Combined Length of Manubrium and Mesosternum of Sternum Measured by CT-VRT in Southwest Han Population.
Ying-zhen LUO ; Meng TU ; Fei FAN ; Jie-qian ZHENG ; Ming YANG ; Tao LI ; Kui ZHANG ; Zhen-hua DENG
Journal of Forensic Medicine 2015;31(3):196-199
OBJECTIVE:
To establish the linear regression equation between body height and combined length of manubrium and mesostenum of sternum measured by CT volume rendering technique (CT-VRT) in southwest Han population.
METHODS:
One hundred and sixty subjects, including 80 males and 80 females were selected from southwest Han population for routine CT-VRT (reconstruction thickness 1 mm) examination. The lengths of both manubrium and mesosternum were recorded, and the combined length of manubrium and mesosternum was equal to the algebraic sum of them. The sex-specific linear regression equations between the combined length of manubrium and mesosternum and the real body height of each subject were deduced.
RESULTS:
The sex-specific simple linear regression equations between the combined length of manubrium and mesostenum (x3) and body height (y) were established (male: y = 135.000+2.118 x3 and female: y = 120.790+2.808 x3). Both equations showed statistical significance (P < 0.05) with a 100% predictive accuracy.
CONCLUSION
CT-VRT is an effective method for measurement of the index of sternum. The combined length of manubrium and mesosternum from CT-VRT can be used for body height estimation in southwest Han population.
Asian People
;
Body Height
;
Female
;
Forensic Anthropology
;
Humans
;
Linear Models
;
Male
;
Manubrium/anatomy & histology*
;
Regression Analysis
;
Sternum/anatomy & histology*
;
Tomography, X-Ray Computed
6.A clinicopathological study on nonalcoholic steatohepatitis.
Zhen Wei LANG ; Zhong Jie HU ; Si Kui WANG ; Li Jie ZHANG ; Xin MENG ; Chen Zhao SONG
Chinese Journal of Hepatology 2003;11(2):81-83
OBJECTIVETo observe the pathological and clinical characters of nonalcoholic steatohepatitis (NASH).
METHODSLiver biopsy tissues taken from 97 patients negative for common viral detection with serological test and immunohistochemistry as well as in situ hybridization, were observed by routine light microscopic examination. And the clinical data of patients with NASH was analyzed.
RESULTSAmong the chronic hepatitis patients with unknown etiology, the detection rate of NASH was 15.5% (15/97). The characteristic lesions in NASH patients included macrovesicular steatosis in zone 3 of lobules, hepatocytes ballooning degeneration, lobules diffused with acute and chronic inflammation, and perisinusoidal fibrosis. Grading and staging according to Brunt's method, histological lesions could be accounted for G1S1 in 7 patients, G2S2 in 3 patients, G3S1 in 4 patients and G3S2 in 1 patient. There were 14 patients with mild to moderate elevation of transaminase, 10 with hyperlipemia, 8 with diabetes and 9 with fatty liver by ultrasonography.
CONCLUSIONNonalcoholic steatohepatitis is a common form of unknown etiology chronic liver disease with certain clinic-pathological features.
Adult ; Biopsy ; Fatty Liver ; pathology ; Female ; Humans ; Liver ; pathology ; Male ; Middle Aged
7.Danshen Modulates Nrf2-mediated Signaling Pathway in Cisplatin-induced Renal Injury
CAO SI-SI ; YAN MIAO ; HOU ZHEN-YAN ; CHEN YING ; JIANG YUN-SHENG ; FAN XIN-RONG ; FANG PING-FEI ; ZHANG BI-KUI
Journal of Huazhong University of Science and Technology (Medical Sciences) 2017;37(5):761-765
Danshen,an efficacious agent for cardiovascular diseases,has been found to play an essential role in kidney injury.In the present study,the effect of Danshen on cisplatin-induced renal dysfunction was investigated in a mouse model.Danshen was administered to mice at a dose of 3 g/kg 4 days before and 3 days after cisplatin treatment.A single intraperitoneal injection of 20 mg/kg cisplatin was used to induce nephrotoxicity.The mice were sacrificed 72 h after cisplatin intoxication.Biochemical parameters including serum creatinine and blood urea nitrogen were analyzed.Histopathological changes of kidney tissues were detected using HE staining.Antioxidant enzymes (GSH-Px and SOD) and peroxidative product (MDA) were detected.Protein expressions of Nrf2 and its target genes including HO-1 and NQO1 were measured by Western blotting.The results showed that pretreatment with Danshen significantly reduced serum creatinine and blood urea nitrogen in the cisplatin-treated mice.Histopathological examination showed that Danshen mitigated the renal damage induced by cisplatin.Moreover,Danshen restored the activities of antioxidant enzymes (GSH-Px and SOD) and normalized the MDA contents in renal tissues.Western blotting revealed that Danshen enhanced the expressions of Nrf2 and its target genes in cisplatin-exposed mice.It was suggested that Danshen protects against the cisplatin-induced renal impairment in the mice,which is potentially associated with the upregulation of Nrf2-mediated signaling pathway.
8.Effect of occupational stress on mental health.
Shan-fa YU ; Rui ZHANG ; Liang-qing MA ; Gui-zhen GU ; Yan YANG ; Kui-rong LI
Chinese Journal of Industrial Hygiene and Occupational Diseases 2003;21(1):16-19
OBJECTIVETo study the effect of job psychological demands and job control on mental health and their interaction.
METHODS93 male freight train dispatchers were evaluated by using revised Job Demand-Control Scale and 7 strain scales. Stepwise regression analysis, Univariate ANOVA, Kruskal-Wallis H and Modian methods were used in statistic analysis.
RESULTSKruskal-Wallis H and Modian methods analysis revealed the difference in mental health scores among groups of decision latitude (mean rank 55.57, 47.95, 48.42, 33.50, P < 0.05), the differences in scores of mental health (37.45, 40.01, 58.35), job satisfaction (53.18, 46.91, 32.43), daily life strains (33.00, 44.96, 56.12) and depression (36.45, 42.25, 53.61) among groups of job time demands (P < 0.05) were all statistically significant. ANOVA showed that job time demands and decision latitude had interaction effects on physical complains (R(2) = 0.24), state-anxiety (R(2) = 0.26), and daytime fatigue (R(2) = 0.28) (P < 0.05). Regression analysis revealed a significant job time demands and job decision latitude interaction effect as well as significant main effects of the some independent variables on different job strains (R(2) > 0.05).
CONCLUSIONJob time demands and job decision latitude have direct and interactive effects on psychosomatic health, the more time demands, the more psychological strains, the effect of job time demands is greater than that of job decision latitude.
Humans ; Mental Health ; Occupational Diseases ; psychology ; Regression Analysis ; Stress, Psychological ; psychology
9.Stature Estimation for Sichuan Han Female Based on Whole-spine X-ray Photo-graphy
Jing-Hui CUI ; Ying-Zhen LUO ; Fei FAN ; Zhao PENG ; Li-Ping DENG ; Kui ZHANG ; Zhen-Hua DENG
Journal of Forensic Medicine 2018;34(1):23-26,32
Objective To establish regression models of stature estimation for Sichuan Han female by the measurement of total vertebral column length in the frontal and lateral X-ray films of whole-spine. Methods The frontal and lateral X-ray films of whole-spine were collected from 200 Sichuan Han fe-males by large flat-panel multi-functional universal radiography and fluoroscopy system. The data and mean values of frontal and lateral total vertebral column length were measured and calculated in all the samples, respectively. The relationship of combined multi-markers and stature were analysed by linear regression analysis, and the mathematical models of stature estimation were established. The data of 30 new samples were selected and inputted for verifying the accuracy of the mathematical models. Results The total vertebral column length showed a good correlation with stature, and the mean values of the frontal and lateral X-ray films of total vertebral column length had the highest correlation coefficients. Three established linear regression equation models were statistically significant(P<0.05), and the equa-tion established with lateral total vertebral column length showed the highest accuracy. Conclusion The stature estimation by the measurement of total vertebral column length has high accuracy.
10.Separation and identification of Taxol in the crude extracts of Taxus cuspidata and its callus culture with HPLC-ESI-MS/MS.
Jie ZHANG ; Ji-cheng DUAN ; Zhen LIANG ; Wei-bing ZHANG ; Li-hua ZHANG ; Yu-shu HUO ; Yu-kui ZHANG
Acta Pharmaceutica Sinica 2006;41(9):863-866
AIMTo study the MS/MS fragmentation mechanism of Taxol, and based on it to establish HPLC-ESI-MS/MS technique to separate and identify Taxol in the crude extracts of Taxus cuspidata and its callus culture, consequently to provide a fast and credible method for the analysis of Taxol in natural products.
METHODSOptimized the HPLC-ESI-MS/MS parameters for the sample analysis, and then discussed the ionization and cleavage mechanism of Taxol in ESI-MS and ESI-MS/MS, finally identified the Taxol in the samples with retention time, molecular weight and MS/MS spectra.
RESULTSElucidated the MS/MS fragmentation mechanism of Taxol, and developed HPLC-ESI-MS/MS method to analyze Taxol in the two samples.
CONCLUSIONThe HPLC-ESI-MS/MS method is rapid, highly sensitive and specific, so it is suitable for the separation and identification of Taxol in natural products.
Chromatography, Liquid ; methods ; Paclitaxel ; chemistry ; isolation & purification ; Plant Extracts ; chemistry ; Plants, Medicinal ; chemistry ; Reproducibility of Results ; Spectrometry, Mass, Electrospray Ionization ; methods ; Tandem Mass Spectrometry ; Taxus ; chemistry