2.Changes in Neuropeptide Y Y1 Receptor mRNA Level in the Infarcted Myoc ardial Tissues of Rats
Liming LU ; Xiangying SUN ; Jun WANG ; Dezhi TIAN ; Yu ZHEN ; Yichun ZHU ; Hedner THOMAS ; Tai YAO
Chinese Journal of Hypertension 2001;9(2):134-137
Aim To investigate the change in neuropeptide Y(NPY) Y1 receptor level in infarcted myocardium tissues of (MI) rats. Method MI was induced by ligating the left descending anterior coronary artery (LAD) in the heart of rats. The techniques of reverse transcription polymerase chain reaction (RT-PCR) were used to identify the exist of NPY Y1 receptor mRNA in myocardial tissues. The technique of semi-quantitative PCR wa s used to observe the change of NPY Y1 receptor mRNA level. Results NPY Y1 receptor mRNA distributed in the heart tissues of rat. Compared with sham operated rats ,the NPY Y1 receptor mRNA level both in infarction area and non-infarction area was increased significantly after MI fo r 1d and 3d. The NPY Y1 receptor mRNA level in the heart tissues of sham-op erated rats was also significantly increased compared with that in control rats . Conclusion These results suggest that MI may result in increase NPY Y1 receptor level in the heart tissues of rats. Stre ss stimulation such as surgery may also increase the NPY Y1 receptor level in the heart.
3.Screening reported mania symptoms by two self-rating questionnaires from outpatients with depressive disorders in a general hospital
Xu ZHANG ; Zheng LU ; Wenyuan WU ; Haisong CUI ; Meilan LIU ; Jun YAO ; Zhen GUO ; Qingwei LI
Chinese Journal of Behavioral Medicine and Brain Science 2011;20(7):658-661
Objective To explore the prevalence of self reposed mania/hypomania symptoms of depressive disorders and the difference between the two self-rating symptoms questionnaires in setting of psychiatric clinic of a general hospital.Methods 102 outpatients who were diagnosed with depressive disorders by ICD-10 in department of psychiatry of Tongji Hospital of Tongji University were continuously investigated and fulfilled the Chinese Version mood disorder questionnaire(CV-MDQ)and the Chinese Version 32 items hypomania check list(CVHCL-32).The positive mania symptoms were elevated with at least seven positive mania items reported by the CVMDQ.The positive hypomania symptoms were elevated with at least fourteen positive hypomania items reported by the CV-HCL-32.Results The internal consistency(Cronbach alpha)of the CV-MDQ was 0.808(95% CI=0.767~0.845,P<0.01).The internal consistency(Cmnbach alpha) of the CV-HCL-32 was 0.916(95% CI=0.898~0.930,P<0.01).11 patients(10.8%) reported positive mania symptoms by the CV-MDQ.14 patients (13.7%)had been reported positive hypomania symptoms through the CV-HCL-32.The ability of discriminating mania or hypomania between the two scales was significantly different(Kappa=0.227,P<0.05).Compared to the patients who were reported negative hypomania symptoms by the CV-HCL-32.the 11 patients with positive hypomania symptoms by the CV-HCL-32 had much earlier age in first episode(35.0 vs 50.5,z=-2.065,P<0.05),much longer months in total disease course(60.0 vs 22.0,z=-2.102,P<0.05)and present episode (12.0 vs 6.0,z=-2.180,P<0.05),and much higher frequency of relapse(2.5 vs 1.0,z=-2.168,P<0.05),but no significant differences at age,gender and education.No significant differences appeared between CV-MDQ positive and negative group.Conclusion Mania or hypomania symptoms may be screened by CV-MDQ and CV-HCL-32 from the outpatients with depressive disorders who are diagnosed by ICD-10 in general hospital.whether CV-HCL-32 is superior to CV-MDQ when screening bipolar Ⅱ disorder is worthly further study.
4.Identification of Major Metabolites of Salbutamol in Swine Urine and Plasma Using Ultra-High Performance Liquid Chromatography-Electrospray-Time of Flight-Mass Spectrometry
Xu GU ; Yiming LIU ; Ting YAO ; Huale SHI ; Jun LI ; Zhen ZHAO ; Yuchang QIN
Chinese Journal of Analytical Chemistry 2014;(11):1692-1696
Theexcretionofurinaryandplasmametabolitesofsalbutamolwasstudiedusingultrahigh performance liquid chromatography electrospray time-of-flight mass spectrometry, after a single intragastric gavaged dose administration with salbutamol. The software of Agilent MassHunter Metabolite ID was employed to find and identify the chemical structure of metabolites of salbutamol. Five metabolites from salbutamol were identified. Metabolites identified in swine urine included glucuronide conjugate salbutamol, N-oxide-salbutamol, hydroxyl-salbutamol, methoxyl-salbutamol and dehydrated hydroxyl-salbutamol. Whereas, only the parent drug, glucuronide conjugate salbutamol and dehydrated hydroxyl-salbutamol were observed in swine plasma.
5.Scanning the SRSF2 gene mutations in acute myeloid leukemia by using high-resolution melting analysis
Dongming YAO ; Jing YANG ; Zhen QIAN ; Lei YANG ; Gaofei XIAO ; Jiang LIN ; Hong GUO ; Jun. QIAN
The Journal of Practical Medicine 2016;32(7):1169-1172
Objective To study the SRSF2 mutations in acute myeloid leukemia (AML) patients by using high-resolution melting analysis (HRMA). Methods PCR-HRMA analysis was performed to screen SRSF2 mutations in 140 cases with AML, and the direct DNA sequencing was used to confirm the HRMA results. Results Five percent (7/140) of AML patients were found with heterozygous SRSF2 mutations, including one case of P95R mutation, two case of P95L mutation, and four cases of P95H mutation, the above mutations were confirmed by direct DNA sequencing. The maximal sensitivity of HRMA in detecting SRSF2 mutation was close to 10%. There were no difference in gender, age and blood parameters among cases with or without SRSF2 mutations (P > 0.05). The overall survival (OS) of patients with SRSF2 mutations was inferior to those without SRSF2 mutations in AML patients (P=0.016). Conclusions HRMA analysis was a convenient, rapid, specific, high-throughput technique for scanning of SRSF2 gene mutations in AML patients. SRSF2 mutation may predict the adverse prognosis in AML patients.
6.The tolerance and safety of nasogastric tube feeding and naso-jejunum tube feeding and incidence of aspiration pneumonia in ICU enteral nutrition patients
Yanjie ZHANG ; Zhen YU ; Jingye PAN ; Jiangao YAO ; Xifang LIN ; Jifang YU ; Jun SONG
Parenteral & Enteral Nutrition 1997;0(04):-
0.05).Conclusion: Enteral nutrition may improve the nutrition status.The naso-jejunum tube feeding has no advantage to nasogastric tube feeding in our patients.The route of enteral nutrition should be selected according to the individual condition and local nosocomial experience.
7.Percutaneous portal vein embolization in treatment of primary hepatocellular carcinoma
Jing-Yao HUANG ; Wei-Zhu YANG ; Na JIANG ; Jian-Jun LI ; Qu-Bing ZHEN ;
Journal of Interventional Radiology 2006;0(11):-
Objective To evaluate the clinical value of percutaneous transcatheter selective right portal vein embolization(PVE)in treatment of primary hepatocellular carcinoma.Methods Twelve patients with unresectable hepatocellular carcinoma were treated with right percutaneous transcatbeter PVE under fluoroscopic guidance.Left hepatic lobe volume was measured by CT before and after PVE.Portal vein pressures and changes of liver function were also detected before and after the embolization.Results Right portal vein was embolized successfully in all 12 patients with compensatory hypertrophy of left hepatic lobe.Right hepatic lobe was successfully resected in 3 patients.There were no evidence of hepatic dysfunction and portal hypertension after PVE and also without complication.Conclusions Percutaneous transcathter portal vein branch embolization can induce atrophy of the embolized lobes with compensatory hypertrophy of the remnant liver,providing another operation chance for patients with unresectable hepatocellular carcinoma.
8.Clinicopathological analysis of 34 cases of primary small intestine lymphoma
jun-liang, YAO ; xing-zhi, NI ; yan-ying, SHEN ; zi-zhen, ZHANG ; zi-li, XIAO
Journal of Shanghai Jiaotong University(Medical Science) 2006;0(05):-
Objective To analyse the clinicopathological features of primary small intestine lymphoma(PSIL), and explore the relationship between clinical stage,histological findings,therapeutic modality and prognosis. Methods The clinical data of 34 cases of PSIL were collected,the pathohistological features and results of immunohistochemical examinations were obtained,and the follow-up findings were adopted for comprehensive analysis. Results Among these 34 cases of PSIL,abdominal pain or discomfort,gastrointestinal bleeding and abdominal mass were the predominant symptoms.PSIL mainly involved ileum,especially the bottom of ileum and ileocecal area.Among the 26 patients with follow-up for more than one year,the 1-year survival rate was significantly higher in patients without tumor perforation than those with tumor perforation(76.2% vs 20.0%)(P
9.Case Report and Review of Literature of Child Subcutaneous Panniculitis-Like T-Cell Lymphoma
xiao-jun, YUAN ; qin, ZHANG ; xiao-hong, YAO ; zhen, WANG ; qi, SHENG
Journal of Applied Clinical Pediatrics 2006;0(24):-
Objective To analyze the clinical and histopathologic characteristics in children with subcutaneous panniculitis-like T-cell lymphoma(SPTCL),and explore the pathological diagnosis and differential diagnosis,in order to boost paediatricians to better understanding the disease.Methods One case was diagnosed SPTCL with over 2 years protracted course of fever and multiple skin lesions.The evolvement of clinical presentation,the misdiagnosed experience,the histopathological features,the immunohistochemical results and T cell receptor(TCR)gene cloning rearrangement were observed.The related literatures published were reviewed.Results Skin biopsy showed that the histopathologic findings were limited within subcutaneous fatty tissue,the atypical lymphocytes characteristically rimmed individual fat cells in a lace-like pattern.Immunophenotypic studies showed CD45,CD45RO,CD3,CD5,T cell intracellular antigen-1(TIA-1)and perforin usually expressed possitive,while CD10,CD20,CD56,CD68,epithelial membrane antigen(EMA)and cytokeratin(CK)were negative,implying tumor cells derived from T-cell.The results of TCR gene rearrangement as following:IgH FR2(+),FR3A(-);TCR ? JVI(-),JVII(+);TCR? JD1(-),JD2(-).Although the protocol of children's T-cell Non-Hodgkin lymphoma was administrated,the treatment outcome was poor.Conclusion SPTCL is a special primary cutaneous lymphoma with poor prognosis,skin biopsy of suffered lesion is an important method for the diagnosis of SPTCL children with unclear recurrent fever and multiple skin lesions.
10.Knee replacement of the treatment of malignant tumor of the proximal tibia and early follow-up.
Zhen-Jun YAO ; Chi ZHANG ; Zheng-Dong CAI ; Zheng-Rong CHEN
Chinese Journal of Surgery 2004;42(12):750-753
OBJECTIVETo discuss the clinical effects of using rotating hinge prosthesis in treating primary malignant tumor of the proximal tibia.
METHODSAfter the malignant tumor of the proximal tibia was extensively resected or radically resected, rotating hinge prosthesis replacement was performed for reconstruction, while neoadjuvant chemotherapy was used in treating the osteosarcoma.
RESULTSThe average follow-up period was 4.2 years. There was no local recurrence and metastasis in 22 patients, though there was recurrence in 8 patients. The MSTS functional scores of all different items 4 years after the operations were above 3, which showed the clinical results were satisfactory.
CONCLUSIONSIt is an efficacious method for the treatment of malignant tumor of the proximal tibia by using rotating hinge prosthesis. It has the advantage of early recovery of joint function.
Adolescent ; Adult ; Arthroplasty, Replacement, Knee ; methods ; Bone Neoplasms ; surgery ; therapy ; Combined Modality Therapy ; Female ; Follow-Up Studies ; Humans ; Knee Prosthesis ; Male ; Middle Aged ; Tibia ; surgery