0.05).The FMPV of the B?448 AA plus GA was higher than that of the GG type in obesity group(P0.05).Conclusions The simple obesity in children is associated with Fg and FMPV,which have become the high risk factors of the disease of heart and brain.The Fg B?448 G/A gene polymorphism may be an accumulative efficiency gene of children with simple obesity by influencing FMPV.

OBJECTIVETo observe effect and mechanism of n-Butanol lysate of alcohol extracts from Actinidia rufa root (monomer of R6,R8).

METHODTunel, Wright's stain with Giemsa's stain dyeing, and Hoechst 33258-PI double dyeing assay were used to detect the apoptosis of SGC7901 tumor cells treated with R6, R8. The SGC7901 tumor cells were randomly divided into control group and two treatment groups administered 0.05 g x L(-1) R6, R8, respectively, for 72 h). FCM assay was used to detect the apoptosis. Agarose electrophoresis assay was used to detect DNA strand break of tumor cells and reveal anti-tumor action mechanism.

RESULTThe apoptosis percentage of the tumor cell in 24 h, 48 h, 72 h was (17.08 +/- 2.78)% , (29.68 +/- 2.96)%, (52.46 +/- 3.81)%; (14.75 +/- 2.14)%, (27.35 +/- 3.79)%, (45.64 +/- 5.24)%, respectively, for the treatment group, significantly higher than that in the control group (1.94 +/- 1.55)%, (2.78 +/- 1.84)%, (11.8 +/- 2.79)% (P < 0.01) by tunnel assay. Wright's stain with Giemsa's stain dyeing assay, Hoechst 33258-PI and FCM double dyeing assay showed same action. R6 and R8 had the effect of inducing the DNA histogram of tumor cells (P < 0.01).

CONCLUSIONThe anti-tumor mechanisms may be associated with inducing the injury of DNA and stimulating apoptosis.

Actinidia ; chemistry ; Apoptosis ; drug effects ; Cell Line, Tumor ; Drugs, Chinese Herbal ; chemistry ; pharmacology ; Flow Cytometry ; Humans ; Immunohistochemistry ; Plant Roots ; chemistry

OBJECTIVETo investigate the expression of matrix metalloproteinase-2 (MMP-2), matrix metalloproteinase-9(MMP-9), transforming growth factor beta(1)(TGF-beta(1)) and IV-collagen (C-IV) in kidneys of diabetic rats.

METHODSRat diabetic model was induced by streptozotocin (70 mg/kg), and kidneys were examined pathologically and the expressions of MMP- 2, MMP-9, TGF-beta(1) and C-IV were studied by immunohistochemistry. The results were analyzed by imaging quantitative analysis technique.

RESULTImmunoreactive MMP-2 and MMP-9 were mainly expressed in the mesangial cells, endothelial cells, parietal layer of Bowman's capsule and tubular cells. The expression of MMP-2 was significantly weaker in the glomeluri of diabetic rats than that of the control animals (P<0.05), while the expression of TGF-beta(1) and C-IV in the glomeluri of diabetic rats was significantly stronger than that of the controls (P%lt;0.05). The expression of MMP-9 didn't show significant different in glomeluri of the two groups (P>0.05).

CONCLUSIONExpression of MMP-2 in glomeluri is decreased in diabetic rats, which may be related to the increased TGF-beta(1) and in turns promote the accumulation of C-IV.

Animals ; Collagen Type IV ; analysis ; Diabetes Mellitus, Experimental ; enzymology ; pathology ; Female ; Immunohistochemistry ; Kidney ; enzymology ; pathology ; Male ; Matrix Metalloproteinase 2 ; analysis ; Matrix Metalloproteinase 9 ; analysis ; Rats ; Rats, Sprague-Dawley ; Transforming Growth Factor beta ; analysis ; Transforming Growth Factor beta1

Objective To explore the plausibility of laryngeal preservation in salvage surgery of recurrent laryngeal carcinoma.Methods Comprehensive clinical and radiological assessments on a specific group of patients with recurrent laryngeal cancer and their relapsed tumors were carried out to determine the methods and indications of salvage surgery for preservation of laryngeal functions.Salvage surgery with preservation of larynx was performed in a selective of 36 laryngeal cancer patients with relapsed tumors in their larynges after radiotherapy or partial laryngectomies.All patients were regularly followed up after discharge from the hospital. Postoperative survival was calculated by Kaplan-Meier's method.Results Among various laryngeal preservation regimens given,a second transoral laser surgery was carried out in 4 cases,horizontal hemilaryngectomy in 4 cases,vertical hemilaryngectomy in 13 cases and Majer-Piquet's operation in 15 cases,respectively.Simultaneous neck dissection was conducted in 18 cases.Postoperative complications were encountered in 6 cases,most common of which were local infection and laryngeal fistula with no hospital mortality and other major morbidities.Five cases with immediate postoperative complicatios were cured with proper management.The remained one case undergone vertical partial laryngectomy for recurrence after tumor resection via laryngeal fissure received postoperative radiation due to positive surgical margin,and complicated laryngeal atresia and obstruction with loss of his laryngeal functions, Local recurrence was observed in 5 cases and simultaneous locoregional recurrence was seen in 1 case,with the resulting local control rate of 83.3％ (30/36).The 3-year postoperative survival rate was 80.6％.The cause of death was tumor recurrence in 3 cases,distant metastasis in 3 cases and non-tumor-associated disease (heart attack) in 1 case,respectively.Conclusions Salvage surgery with laryngeal preservation can be carried out in the selected cases with early or locally-confined recurrent lesions within the larynx,on the condition that the recurrent tumors be assessed thoroughly and indications for associated surgical techniques be evaluated properly.

Objective To investigate the effect of tigecycline on multi-drug resistant Acinetobacter baumannii ( MDR -AB ) adeB gene expre-ssion and its clinical significance.Methods A total of 60 clinical iso-lates of multidrug-resistant Acinetobacter baumannii were selected, from which 15 experimental strains with positive result to ciprofloxacin ( CIP) efflux pump encoded gene were screened.The minimum inhibitory con-centration ( MIC) before and after given tigecycline was determined using agar dilution assay, the gene of the strains with large change of MIC value was sequenced and compared.Results The lowest MIC of tigecy-cline to MDR-AB was 2 mg? mL-1 .The MDR-AB′s MIC of tigecy-cline at concentration of 0 , 0.62 mg? mL-1 and ciprofloxacin at 1.25 , 2.5 mg? mL-1 were significantly different ( P<0.05).After tigecycline treatment, adeB gene segment had 15 bases mutation, 5 amino acids are replaced and 4 mutant amino acid had no substitution.The adeB gene expression levels before and after the treatment of tigecyclin were (2.51 ±0.72) and (0.87 ±0.21), statistically significant (P<0.05). Conclusion Tigecycline inhibits MDR -AB efflux pump adeB gene expression, lowers adeB expression levels. Other agents with its combination for the treatment of MDR-AB might improve the clinical efficacy.

Adolescent ; Adult ; China ; epidemiology ; Disease Outbreaks ; Female ; Humans ; Incidence ; Leptospirosis ; epidemiology ; mortality ; Male ; Middle Aged

OBJECTIVETo evaluate the prognostic significance of a new grading and scoring system (based on the new IASLC/ATS/ERS classification) in stage I pulmonary adenocarcinoma, as compared with the WHO grading system.

METHODSThe clinicopathologic characteristics of 125 patients with stage I pulmonary adenocarcinoma primarily treated by surgical resection were reviewed retrospectively. All cases were classified according to the new IASLC/ATS/ERS classification and graded into three prognostic groups based on the new classification, the Sica scoring system and the WHO grading system, respectively. The differences in prognosis of the three groups were analyzed.

RESULTSThere was a statistically significant correlation between the new grading system and the WHO grading system (P = 0.000). Both of them showed negative correlation with overall survival. The new scoring system however better correlated with disease recurrence and/or metastasis (P = 0.855, P = 0.073 versus P = 0.011). According to univariate Log-rank test, the prognosis correlated with tumor size (P = 0.004), clinical stage (P = 0.000), the WHO grading (P = 0.020), the new grading system (P = 0.000), the new scoring system (P = 0.000), vascular invasion (P = 0.021), and recurrence and/or metastasis (P = 0.000). The Cox regression analysis demonstrated that clinical stage (P = 0.014), the new grading system (P = 0.047), the new scoring system (P = 0.043), and recurrence and/or metastasis (P = 0.018) were significantly independent poor prognostic factors.

CONCLUSIONSThe new grading and scoring system shows good correlation with the WHO grading system. Compared with the WHO grading system, the new scoring system based on the new IASLC/ATS/ERS classification provides valuable information in categorizing stage I pulmonary adenocarcinoma cases with different risks of disease recurrence, tumor metastasis and prognosis.

Adenocarcinoma ; classification ; pathology ; surgery ; Adenocarcinoma, Mucinous ; pathology ; surgery ; Adult ; Aged ; Aged, 80 and over ; Carcinoma in Situ ; pathology ; surgery ; Female ; Follow-Up Studies ; Humans ; Lung Neoplasms ; classification ; pathology ; surgery ; Male ; Middle Aged ; Neoplasm Grading ; methods ; Neoplasm Invasiveness ; Neoplasm Metastasis ; Neoplasm Recurrence, Local ; Neoplasm Staging ; Prognosis ; Research Design ; Retrospective Studies ; Societies, Medical ; Young Adult

Animals ; Cell Line ; Dendritic Cells ; immunology ; Hepatitis B virus ; immunology ; Mice ; Mice, Inbred BALB C ; T-Lymphocytes, Cytotoxic ; immunology ; Uric Acid ; pharmacology

Adaptor Proteins, Signal Transducing ; genetics ; B-Cell CLL-Lymphoma 10 Protein ; Chromosomes, Human, Pair 11 ; genetics ; Chromosomes, Human, Pair 18 ; genetics ; Gene Rearrangement, B-Lymphocyte ; Humans ; In Situ Hybridization, Fluorescence ; methods ; Lymphoma, B-Cell, Marginal Zone ; genetics ; Paraffin Embedding ; Translocation, Genetic

OBJECTIVETo observe the protective effect of Panax notoginseng saponins (PNS) on the level of synaptophysin ptotein in brain in rat model with Alzheimer's disease (AD).

METHODThe AD rat models were established by intra-peritoneal injection of D-galactose combined with excitatory neurotoxin ibotenic acid injection into bilateral nbM. The activity and content of synaptophysin protein in brain were determined by immunohistochemistry analysis.

RESULTPNS could reduce the lesion of level of synaptophysin protein in brain, as compared with those of model group's rats.

CONCLUSIONPNS plays a protective role by reducing down of the level of synaptophysin protein in brain in lesion of AD animal model.

Alzheimer Disease ; chemically induced ; metabolism ; pathology ; Animals ; Basal Nucleus of Meynert ; drug effects ; pathology ; Brain ; metabolism ; pathology ; Galactose ; toxicity ; Ginsenosides ; isolation & purification ; pharmacology ; Ibotenic Acid ; toxicity ; Neuroprotective Agents ; isolation & purification ; pharmacology ; Panax ; chemistry ; Plants, Medicinal ; chemistry ; Rats ; Rats, Wistar ; Synaptophysin ; metabolism