BACKGROUND:Large randomized controled clinical trials have shown that drug-eluting stents has a lower
restenosis rate compared with bare-metal stents, but the drug-eluting stents cannot reduce major cardiovascular events and death rates, and long-term folow-up of clinical registries show the drug-eluting stents even may
increase late stent thrombosis events.
OBJECTIVE:To evaluate the incidence of stenosis and adverse reactions after treatment with biodegradable, non-biodegradable drug-eluting stents and bare-metal stents in patients after myocardial infarction.
METHODS:We retrospectively analyzed the clinical data of 236 cases of acute myocardial infarction, in which 79 cases were treated with biodegradable sirolimus-eluting stents, 83 cases treated with non-biodegradable
sirolimus-eluting stents, and 74 cases treated with bare metal stents. Then, we compared late lumen loss and restenosis within 12 months after stent implantation and major adverse cardiac reactions within 24 months after stent implantation.
RESULTS AND CONCLUSION: After 12 months, the late lumen loss of the bare stent group was significantly higher than that of biodegradable and non-biodegradable sirolimus-eluting stent groups (P < 0.05), but the
difference in stent restenosis rate (P > 0.05). After 24 months, there were no differences in death, cardiac death, recurrent myocardial infarction, target vessel revascularization, target lesion revascularization, major adverse
cardiac events and stent thrombosis events among the three groups. These three kinds of stents need to be
further studied in their long-term efficacy and safety as percutaneous coronary intervention for acute ST-segment elevation myocardial infarction.

Cardiomyopathy, Dilated ; etiology ; pathology ; therapy ; Humans ; Myocardium ; pathology

Objective To observe inhibitory effects of Ginsenoside on the proliferation cultured stem cells of human rectal carcinoma in vitro.Methods Tumor stem cells of rectal carcinoma in human were cultured in vitro and divided into saline water group,reltitrexed group(3mg/mL),and Ginsenoside(25μg/mL)plus reltitrexed(3mg/mL) group,with dosage of qd and for 2 weeks.Cells growth was observed under the microscope,and immunofluorescence staining was used to detect CD +133 expression of rectal carcinoma cells.MTT colorimetric method was taken to measure inhibitory of Ginsenoside on proliferation of tumor stem cells of rectal carcinoma.Apoptosis of tumor stem cells of rec-tal carcinoma lead by Ginsenoside was observed by DAPI staining.Results Part of cells were growing up like ball and CD +133 immunofluorescence staining was positive.Inhibitory role of Ginsenoside plus reltitrexed on CD +133 cells of rectal carcinoma appeared on the first day,which was bigger than that of retitrexed by MTT method (P =0.03).Com-pared with the retitrexed group,Gisenoside combined with reltitrexed reduced CD +133 cells expression of rectal carcino-ma,in which optical density and area density of CD +133 cells were obviously decreased with statistical significance (P =0.007,P =0.006,respectively).Conclusion Gisenoside plays the remarkably inhibitory role on proliferation of CD +133 cells of rectal carcinoma.

Objective To investigate the effects of KangAi injection on apoptosis and proliferation of rectal cancer Lovo cells in vitro.Methods SubG1 and Annexin -V /PI were used to examine the apoptosis of Lovo cells in vitro.Real -time PCR was used to examine the expression of Caspase -3 and Bcl -2 at mRNA level.Proliferation protein ki -67 was detected by flow cytometry.Results Lovo cells treated with KangAi injection 180μL/mL for 48h increased apoptosis rate from (4.75 ±1.04)% to (45.61 ±5.22)% with SubG1 (t =7.68,P <0.01 ),and (7.28 ±1.99)% to (57.02 ±3.88)% with Annexin -V /PI(t =11.42,P <0.01).Lovo cells treated with KangAi injection 180μL/mL for 48h increased the expression of Caspase -3 at mRNA level(t =4.06,P <0.05),while Bcl-2 decreased(t =5.69,P <0.01 ).Lovo cells treated with KangAi injection 180μL/mL for 48h decreased the expression of ki -67 from (95.79 ±1.66)% to (65.84 ±4.80)%(t =5.90,P <0.01 ).Conclusion KangAi injection can promote apoptosis and inhibit proliferation of rectal cancer Lovo cells in vitro.

Objective To observe the effects of neuromuscular electrical stimulation (NMES) at different frequency on post-stroke pharyngeal dysphagia.Methods Forty-five stroke survivors with pharyngeal dysphagia were randomly divided into treatment group 1,treatment group 2 and treatment group 3,eachof 15 patients.Patients in the treatment group 1,2 and 3 received NMES once,twice and three times a day respectively.All the 3 groups were given conventional swallowing training.The swallowing function classification was conducted before treatment,as well as 1,2 and 4 weeks after the treatment.Before and 2 weeks after the treatment,X-ray examination of swallowing (VFSS) was taken to assess the swallowing function,and the average treating days of the 3 groups were compared.Results The swallowing function classification showed that in treatment group 1 and 2,significant improvement was observed at this time point than that at the previous time point (P＜0.05).In treatment group 3,after 1 and 2 weeks of treatment,significant improvement was found compared to those previously,but there was no difference between after 2 weeks and 4 weeks of treatment.At the same time points,the treatment group 2 and 3 showed higher efficiency than the group 1 (P＜0.05).And compared with the treatment group 2,the treatment group 3 showed higher efficiency after 1 and 2 weeks of treatment (P＜0.05),but there was nosignificant difference after 4 weeks of treatment.Two weeks after the treatment,the average VFSS scores of all groups were significantly higher than those before the treatment (P＜0.05) and that of treatmentgroup 2 (7.600± 1.793) and 3 (8.900± 1.636) was significantly higher than that in treatment group 1 (6.700±1.873),as was that of group 3 compared to group 2.Compared with the treatment group 1 and 2,the average treatment days decreased significantly in treatment group 3 (P＜0.05).Conclusion NMES is effective in treating pharyngeal dysphagia after stroke.And within a certain range (1 to 3 times every day),with the increasing of daily treatment frequency,the effectismore significant and the average time of treatment shortens.

Humans ; Maintenance Chemotherapy ; Multiple Myeloma ; therapy

OBJECTIVE To observe the inhibition of the expression of HIF-1? and VEGF induced by LPS in primary human nasal epithelial cell (HNEC) by dexamethasone. METHODS Epithelial cells of nasal polyps (NP) and inferior turbinate (IT) were cultured without serum under stimulus of LPS 100 ng/ml, IL-1? 20 ng/ml, LPS 100 ng/ml +dexamethasone 13 ng/ml and IL-1? 20 ng/ml+ dexamethasone 13 ng/ml for 3h, 6h and 9h respectively. The expression of HIF-1?, VEGF protein and mRNA derived from epithelial cells was detected by immunocytochemistry and situ hybridization. RESULTS ①The expression of HIF-1? and VEGF increased under the stimulus of LPS and IL-1? with the increasing of time and concentration, especially under LPS 100 ng/ml for 6h (P

Objective To study the functional mechanism of Yougui Pill(Right Restoring Pill)through its effect on cerebral gene of mice models with kidney-yang asthenia caused by excessive physical and sexual activity.Methods There were 10,15,10 male mice in the control,model and treatment groups respectively.The control and model groups were treated with 0.5ml permouse perday distilled water by gavage while the treatment group was given 0.5ml permouse perday suspension of Yougui Pill.In the model and treatment groups,the ratio of male and female was 1∶6,feeding in the same cage,and the males were swimming everyday for 30~40 minutes,4 weeks in succession,to induce the kidney-yang asthenia.The 36K mouse cerebral gene chips were used to measure the brain genes of mice of 3 groups.The intensity ratio of fluorescence signals ≥2 and ≤0.5 were used as the scale to screen out the remarkably different genes.Results In the model and control groups,totally 186 gene with different expressions were screened out,including the 150 up-regulated genes and 36 down-regulated genes.In the treatment and model groups,155 gene with different expressions were screened out,including the up-regulated 64 genes and 91 down-regulated genes.There were 32 genes up-regulated in the model and control groups but down-regulated in the treatment and model groups,mainly involving the inflammation perimmunity and neurotransmissions persignal transduction genes.There were 12 genes down-regulated in the model and control groups but up-regulated in the treatment and model group,mainly involving the hormone related genes of melanin and orphan nuclear receptor.Conclusion Yougui Pill may make the notably down-regulated genes of melanin markedly up-regulated in mice models of kidney-yang asthenia,improving the effect of hormone related orphan nuclear receptor and promoting the cellular proliferation.

The superoxide dismutase (SOD) activity of liver, lung, stomach and kid-ney of rabbits with schistosomiasis (n=10), normal animals with SMAO (n=7), andsham operative group (n=6) were measured. The results showed that SOD activity inanimals of rabbits organ decreased significantly during SMAO shock (P

This review introduces the basal elements and structures of Ussing chamber and it′s applications in the field of intestinal barrier function research.