Objective To discuss the curative effect of carboprost tromethamine in the treatment of uterine atony postpartum hemorrhage.Methods 50 cases of refractory uterine atony postpartum hemorrhage patients in cesarean section were divided into the observation group and the control group,each of 25 cases,the control group was given brace suture treatment,the observation group was given carboprost tromethamine base on the control group,curative effect,24h postpartum hemorrhage,hysterectomy,postoperative recovery and the effect of maternal lactation were compared between two groups.Results There showed no postoperative death,no infection,uterine necrosis,intestinal obstruction and other complications between two groups.Effective rate in the observation group was 85％,which was higher than 68％ in the control group (x2 =3.12,P ＜ 0.05).24h bleeding,uterine resection rate in the observation group were less than those in the control group (t =5.87,x2 =2.85,P ＜ 0.05).Puerperal morbidity between two groups was no statistical significance (P ＞ 0.05).the initial time of lactation,24h,48h lactation scores between two group were not significantly different (P ＞ 0.05).B ultrasound review in 6 weeks postpartum were normal.Conclusion Carboprost tromethamine in the treatment of refractory uterine atony postpartum hemorrhage is better,which can reduce the rate of hysterectomy significantly with safe,efficient,rapid and convenient.

OBJECTIVE: To discuss the identification method of L-asparaginases prepared from E.coliASI.357 and Erwinia carotovora and the optimal enzymatic reaction conditions of potency determination.METHODS: HPLC method and isoelectric focusing electrophoresis were applied for the identification of L-asparaginase from 2 kinds of strain.The effects of category of buffer solution and pH value on enzymatic reaction of potency determination of L-asparaginase were investigated.RESULTS: HPLC chromatogram of L-asparaginases from E.coli ASI.357 was different from that from Erwinia carotovora.The retention time of the peaks were 11.0 min and 11.8 min.The isoelectric point (PI) of L-asparaginase produced from E.coliASI.357 was within 4.65～5.1 and that produced from Erwinia carotovora was within 7.1～8.20.The optimal enzymatic reaction conditions of potency determination of L-asparaginase produced from E.coliASI.35 were Tris-HCl (pH=9.0) as buffer and that produced from Erwinia carotovora was 0.2 mol?L-1 phosphate (pH=8.0) as buffer.CONCLUSION: The isoelectric point (PI) of L-asparaginases produced from 2 kinds of strain is different from each other as well as their optimal enzymatic reaction conditions of potency determination.The L-asparaginases from 2 kinds of strains should be controlled as 2 different categories.

Parathyroid hormone-related protein (PTHrP) and its receptor type 1 (PTH1 R) are extensively expressed in the kidney,where they are able to modulate renal function.Animal and human studies have shown that PTHrP acts as an important mediator of diabetic renal cell hypertrophy by a meehanism which involves the modulation of cell cycle regulatory proteins and TGF-β1.Furthermore,angiotensin Ⅱ (Ang Ⅱ) appears to be responsible for PTHrP upregulation in these conditions.These findings provide novel insights into the well-known protective effects of Ang Ⅱ antagonists in renal diseases,paving the wav for new therapeutic approaches.

Objective To explore the relationship between the protein expression of PCNA and biological behavior science in benignant pituita ry adenoma.Methods The protein expression of PCNA in 58 patien ts with pituitary adenoma were determined by ABC immunohistochemical method.Results The PCNA index was significantly higher in the patients with recurrent pituitary adenomas than in nonrecurrent ones(P<0.05).There was no significantly difference between bleeding and unbleeding group,cystic and noncystic group,large type and unlarge type group(P>0.05 respectively) .Conclusion The protein expressions of PCNA reflected the proli ferative activties of pituitary adenomas, and could be taken as one of the indic ators to evaluate recurrence and prognosis of the tumor.

[Objective] To discuss the effect of point application therapy and massage combined with little dosage of midazolam in the sedation for polydactylism replantation. [Method] Select 82 patients, randomly divide them into control and observation groups, n=41 for each. The control group only took midazolam for sedation;the other one, point application therapy and massage combined with little dosage of midazolam; observe both sedation effect, side effects and comfort degree. [Result] By comparing both groups, the observation group was better than control one on sedation effect, side effect rate and comfort degree. [Conclusion] Taking point application therapy and massage combined with little dosage of midazolam had good sedation and high comfort degree, with little side effects, fit for sedation in polydactylism replantation under continual brachial plexus anesthesia.

Osteoporosis is a major health concern and treatment of primary osteoporosis with anti-osteoporosis medications is needed to reduce fracture risk and burden. Before initiating anti-osteoporosis medications, secondary causes of osteoporosis should be considered and satisfactorily excluded. However, it can be challenging to differentiate primary osteoporosis from secondary osteoporosis, especially in patients with paucity of symptoms or who have less common clinical presentation. Hence, practical tips like Appropriate Care Guide on Osteoporosis forms the basis of initial secondary osteoporosis workup for primary care physicians. Snapshots of secondary osteoporosis are briefly discussed to facilitate “pattern recognition”.

Objective To explore the effect of home care on schizophrenia in rural area in Beijing. Methods A comparative study has been done between 36 patients in home care and 36 patients in hospital. All of the patients were tested with Positive and Negative Symptoms Scale (PANSS) before, 4 and 8 weeks after treatment and Social Disability Screening Scale (SDSS) at 6 months after treatment. Results The PANSS scores of two groups decreased significantly after the treatment (P<0.01). There was no significant difference in the effective rates between the home care group (69.4%) and the hospital group (66.7%). The SDSS scores of the home care group was significantly lower than the hospital group (P<0.01). Conclusion Home care provides an efficient approach to treat schizophrenia in rural community, and it can improve the social function of the patients.

Objective To investigate the feasibility of pig lung cancer model induced by bronchial perfusion of 3,4-benzopyrene. Methods 24 experimental pig were randomly divided into model group and control group, each containing 12 cases. Experimental pigs were under the anaesthetic state, pigs in the model group were given endobronchial infusion of 3,4 - benzopyrene - corn oil mixture, pigs in control group were injected with equal capacity of corn oil.Perfusion 1 times a week for 16 weeks.In week 16,32 and 48,all experimental pig were given lung CT scans, then the lung lesions were observed.After 48 weeks, the pig lung, esophagus, and gastrointestinal tract, liver and brain and other organs were dissected, the presence of tumor formation was observed, and the mass, and experimental pig lung biopsy were given hematoxylin and eosin staining analysis. Results Lung cancers were not found in control group by both CT lung cancers and anatomy. In the model group, pulmonary CT showed space-occupying lesions with different location and size in lungs of 8 pigs, and the space-occupying lesions were confirmed as malignant tumors by pathology, including 3 cases of moderately differentiated adenocarcinoma, 2 cases of highly differentiated squamous cell carcinoma, 1 case of alveolar cell carcinoma,2 cases of adenosquamous carcinoma. Three other pigs and pigs in the control group were not found with tumor by both lung CT and anatomy. Pigs in model group were induced successfully to malignant tumor in 1 year , the total tumor formation rate was 75%,lung tumor formation rate was 66.66%. Conclusion The trachea bronchial perfusion of 3,4- benzopyrene is a simple,safe and reliable way to construct animal models of lung cancer.

OBJECTIVETo investigate whether bortezomib can enhance the sensitivity of human prostate cancer (PCa) cells to natural killer (NK) cell-mediated cytotoxicity, and whether it produces the same effect on different PCa cell lines.

METHODSWe treated androgen-dependent PCa LNCaP cells and androgen-independent PCa DU145 cells with bortezomib at the concentrations of 0, 5, 10, 15, 20 and 25 nmol/L for 24, 48 and 72 hours, and then detected the proliferation and apoptosis of the tumor cells by CCK-8 and Annexin V/PI, respectively.

RESULTSThe proliferation rates of the DU145 cells treated with 15, 20 and 25 nmol/L bortezomib were (82.79 +/-2.04)%, (73.59+/- 2.95)% and (74.16+/- 6. 16)% at 48 hours and (71.24+/- 5.30)%, (51.20+/- 2.91)% and (38.02+/- 2.67)% at 72 hours, and those of the LNCaP cells were (77.04+/- 7.74)% , (42.61 +/- 6.62)% and (23.85 +/-6.04)% at 48 hours and (36.45 +/-7.02)%, (14.94 +/-5.76)% and (11.65 +/-5. 87)% at 72 hours, both significantly inhibited as compared with the control group (P <0.05). At 24 hours, the apoptosis rates of the DU145 cells treated with 15, 20 and 25 nmol/L bortezomib were (14.41 +/- 1.32)% , (16.13 +/- 1.55)% and (14.48 +/- 1.42)% , and those of the LNCaP cells treated with 20 and 25 nmol/L bortezomib were (12.77 +/- 1.28)% and (14. 84 +/- 1.65)% , significantly higher than those of the control group (P <0.05) , and the DU145 cells showed an even higher sensitivity to bortezomib than the LNCaP cells. Bortezomib failed to sensitize these two cell lines to NK cell-mediated cytotoxicity in short-term assay, while long-term assay manifested that the apoptosis rates of DU145 and LNCaP cells after treated with 20 nmol/L bortezomib + NK cells were (41.83 +/- 5.06)% and (30.31 +/- 3.62)% , respectively, significantly higher

CONCLUSIONBortezomib enhances the sensitivity of than those after treated with either bortezomib or NK cells alone (P <0.05). PCa cells to NK cell-mediated cytotoxicity and adds to the effect of current cancer therapies, and it is more efficacious for androgen-independent prostate cancer.

Apoptosis ; drug effects ; Boronic Acids ; pharmacology ; Bortezomib ; Cell Line, Tumor ; Cytotoxicity, Immunologic ; drug effects ; Humans ; Killer Cells, Natural ; drug effects ; Male ; Prostatic Neoplasms ; pathology ; Pyrazines ; pharmacology

Electroencephalography ; Epilepsies, Myoclonic ; diagnosis ; therapy ; Female ; Follow-Up Studies ; Humans ; Infant ; Male ; Prognosis ; Spasms, Infantile ; diagnosis ; Time Factors