OBJECTIVE:To put forward game strategy of drug safety regulation,and to provide reference for decision-making by drug regulation department. METHODS:Based on evolutionary game theory,evolutionary game model of drug safety regula-tion was established,and the stability of evolution during drug safety regulation was analyzed. RESULTS & CONCLUSIONS:There were four possible evolutionary game results of drug safety regulation,including to provide qualified drugs,regulation;to provide unqualified drugs,regulation;to provide qualified drugs,no regulation;to provide unqualified drugs,no regulation. The evolution direction mainly depends on the parameter values involved in the payoff matrix of drug safety regulation. The stable state of drug safety regulation is related to regulation strength,regulation cost,enterprise safety input,probability of accident,acci-dent-related treatment cost,et al. Key strategies to improve drug safety regulation include increasing punishment,improving moni-toring technology,strengthening coordination mechanism,promoting social cohabitation and improving industry self-control.

This paper reports nursing of 11 patients undergoing laparoscopic radical cystectomy with orthotopic ileal neobladder. The nursing points included preoperative bowel care,psychological care,observation of drainage tubes and complications,guidance of pelvic floor muscle training and bladder training with timed voiding. Patients receiving bladder training after catheter removal,and got self-control urination in 2 weeks. Only a patient left urinary incontinence at night occasionally.

Objective:To observe the clinical effects of herbal cake-partitioned moxibustion in the treatment of knee osteoarthritis (KOA).
Methods:A total of 120 cases with KOA were randomly divided into two groups by the random digital table, 60 cases in each group. The herbal cake-partitioned moxibustion (HCPM) group was treated by herbal cake-partitioned moxibustion, once per day and five days per week, with ten sessions as one course. The Western medication (WM) group was treated by the oral administration of Diclofenac Sodium Sustained-release Tablets. The knee functions of the patients were assessed by Western Ontario and McMaster Universities osteoarthritis index (WOMAC) before and after the treatment. The clinical effects were observed in the two groups after four-week continuous treatment.
Results:The total effective rate was 86.7% in the HCPM group and 73.3% in the WM group. The difference in the total effective rate between the two groups was statistically significant (P<0.05). After treatment, WOMAC scores in the two groups were statistically different than those of the same group before the treatment (bothP<0.05); the difference between the two groups were statistically significant (P<0.05).
Conclusion:Herbal cake-partitioned moxibustion and oral administration of Diclofenac Sodium Sustained-release Tablets can improve the knee functions of KOA patients, but the therapeutic effect was better by herbal cake-partitioned moxibustion than by oral administration of Diclofenac Sodium Sustained-release Tablets.

OBJECTIVE To study the correlation between mutations of gyrA and parC genes and ciprofloxacin-(resistant) clinical isolates of Acinetobacter baumannii from Chengdu.METHODS The genes of gyrA and parC DNA in 28 ciprofloxacin-resistant and 2 ciprofloxacin-susceptible isolates of A.baumannii were amplified by PCR and then analyzed by restriction fragment length polymorphism(PCR-RFLP) and DNA sequencing.(RESULTS) Hinf Ⅰ digestion of the gyrA gene products of susceptible isolates(generated) two fragments,but resistant isolates(generated) one fragment.The parC gene products generated 2 fragments.DNA sequencing of 5 resistant isolates revealed mutations in gyrA gene that resulted in amino acid substitutions: Ser83→Leu and Ala88→Thr,especially,Ser83 point mutation accounting for the disappearance of sequence of Hinf Ⅰ.There was not any mutation in(gyrA) of 1 susceptible isolate;the substitution of Ser108→Ile in parC(gene) of 1 susceptible and 1 resistant isolates were identified,the remaining four isolates had more nosense mutations.CONCLUSIONS Compared with parC gene mutations,(gyrA) gene mutations of A.baumannii appears to be the main molecular mechanism responsible for ciprofloxacin resistance.The high-level ciprofloxacin resistance in A.baumannii probably needs a variety of mutations.

Scientific research archives management work is a basic work in the hospital work,establish hospital scientific research management information system,implementation of scientific research archives management informationization can not only realize the scientific research resources sharing,and can greatly improve the scientific quality of service and efficiency.This paper analyzes the necessity of scientific research archives information management,and the main plate and the function of information management system of scientific research archives has carried on the detailed introduction.

[Objective]To prepare a new porous composite artificial bone substitute and evaluate its properties.[Method]Thermally induced phase separation was adopted to prepare the artificial bone made from oyster shell powder and PLLA which were proportionally mixed and its properties as porosity rate,pore size and mechanical strength were assessed.Meanwhile the related variation parameters were examined every 2 weeks in a course of 14 weeks after the slices of CAB and pure PLLA were immersed in NS of 37℃ in vitro,the results of which were compared in statistics.[Result]The average porosity rate of artifical bone with TIPS method was 85.1% and pore sizes ranged 100-300 ?m under the SEM,with better pore connectivity and regulation shape.The average compressive strength was 2.12 MPa.As the immersion prolonged,the regular variation was observed about the mass loss of CAB and the pH alteration of solution,there was statistically difference in the indexes between the two groups(P﹤0.05).[Conclusion]The porosity rate,pore size,mechanical strength and degradation performance in vitro of the artifical bone made by TIPS method can satisfy the requirement of bone substitute.

Objective To investigate the influence of bronchoalveolar lavage (BAL) combined with mechanical ventilation on short－term clinical effect,pathogenic bacteria conversion rate and laboratory index of patients with lung cancer and infection after operation. Methods From October 2014 to October 2017,110 patients with lung cancer and infection after operation in the First People＇s Hospital of Pinghu were chosen and randomly divided into two groups,with 55 patients in each group. The control group received mechanical ventilation,and the observation group received BAL combined with mechanical ventilation. The short － term clinical effect, pathogen clearance rate, the levels of respiratory mechanics index and inflammatory cytokines before and after treatment of the two groups were compared. Results The short－term effective rates of the control group and the observation group were 72. 73% , 94. 54% ,respectively. The pathogen clearance rates of the control group and the observation group were 72. 73% , 96. 36% ,respectively. The short － term effective rate and pathogen clearance rate of the observation group were significantly higher than those of the control group(χ2=9. 87,10. 07,all P＜0. 05). The levels of Raw,PIP and Cydn of the observation group after treatment were ( 8. 15 ± 1. 33 ) cmH2O, ( 14. 15 ± 2. 29 ) cmH2O, ( 33. 27 ± 8. 09)cmH2O,respectively, which were significantly better than before treatment and those of the control group [(12.26±1.87)cmH2O,(22.36 ±3.14)cmH2O,(25.96 ±6.95)cmH2O](t=4.98,6.44,4.67,3.19,3.56, 3. 30,all P＜0. 05). The levels of hs－CRP,TNF－α,IL－8 and PCT of the observation group after treatment were (48. 43 ± 11. 20)mg/L,(11. 05 ± 2. 42) ng/L,(96. 72 ± 12. 09) ng/L,(14. 26 ± 2. 33) ng/L,respectively,which were significantly better than before treatment and those of the control group[(63. 01 ± 15. 42)mg/L,(24. 12 ± 4.75)ng/L,(142.54 ±20.40)ng/L,(20.77 ±4.15)ng/L](t=3.89,3.44,4.16,4.95,2.67,2.51,3.17,3.46, all P ＜0. 05). Conclusion BAL combined with mechanical ventilation in the treatment of patients with lung cancer and infection after operation can efficiently control the respiratory symptoms,accelerate the removal process of pathogenic bacteria, improve the respiratory mechanics and is helpful to reduce the levels of inflammatory cytokines.

Objective To study the effects and current mechanism of low concentration of lidocaine on evoked-bursting (EB) firing of dorsal root ganglion (DRG) neurons in rat model of chronic compression (CCD) of DRG . Methods Twenty-four SD rats were divided into normal control group (n=12) and CCD model group (n=12). CCD group was treated with chronic oppression on L4 and L5 DRG with L shape bar. Normal control group received no treatment. In vivo intracellu?lar recording was used to record the incidence of EB and the effect of lidocaine on subthreshold membrane potential oscilla?tion (SMPO). Patch clamp recording was used to record the effect of lidocaine on persistent sodium current (INaP). Results The incidence of EB increased in CCD group( 45.97%, 57/124), which was significantly different when compared with nor?mal group (χ2=26.810, P<0.01). The magnitude of SMPO, INaP and EB were inhibited in a reversible way by lidocaine (50μmol/L). Conclusion The low concentration of lidocaine might play an analgesic effect in peripheral nervous system by se?lectively inhibiting INap, which participates in SMPO formation.

Objective To investigate the features of HIV-1-specific Cytotoxic T-Lymphocyte (CTL)responses in Chinese.Methods The HIV-1-specific CTL responses were analyzed in an IFN-?ELISPOT assay by using a matrix system containing 820 overlapping peptides spanning the entire HIV-1 Clade B and C consensus sequence.Results The HIV-1-specific CTL response almost clus- tered in Gag and Nef across either HIV-1 Clade B(HIV-1 B)or HIV-1 Clade C(HIV-1 C),while oth- er proteins could also be recognized at different level.In comparison of the response between HIV-1 Clade B and Clade C,the magnitude and frequency was roughly identical with some difference found at single-peptide level.The most frequently recognized peptides of HIV-1 B were located in Nef,GPKEP- FRDYVDRFYKTLR(5/17,29.4%)and Gag,LWVYHTQGYFPDWQNY(5/17,29.4%),while the most frequently recognized peptide of HIV-1 C was located in Gag GPKEPFRDYVDRFFKTLR(6/17, 35.29%).Conclusions HIV-1-specific CTL responses clustered within HIV-1 Gag and Nef in Chinese. However,there was some difference between HIV-1 B and HIV-1 C at single-peptide level.

It is very important to assess the role of suitable social medical support level in operation and adjustment of social medical insurance system.The social medical support level in Shandong Province was measured and analyzed,the coordination of social medical support level and economic development was emphasized with suggestions put forward for the development of system and optimization of policies.