Objective To classify all the plant pollen allergens, each allergen sequence available was compared with protein family database. After that, the appearance and taxonomic distribution of each protein family could be known. When made reference to evolutionary analysis, a regular rule of the distribu-tion of all plant pollen allergens could be concluded. Methods Protein family memberships of each allergen were assigned by comparing the sequences with the Pfam database. Representative members of the most a-bundant pollen allergen families were compared with the Uniprot database using the BLAST server. Acces-sion number of all the interesting homologous could be obtained and all the sequence information could be ac-quired by Batch Entrez. Finally, the evolutionary trees were drawn with the help of MEGA4.0 software. Re-sults One hundred and sixty-eight pollen allergens were classified into 26 protein families. Profilins, pollen _allerg_1 and EF hands constituted the most abundant pollen allergen families. Profilins and EF hands oc-curred in almost all allergenic plant families, whereas allergenic Expansins, FAD_binding proteins, Amb_V allergens and Thaumatins were confined to single taxon. Conclusion It is concluded that the highly con-served sequences of allergens families such as Profilin may be one of the most important reasons of the cross reactivities in allergic diseases. The classification of pollen allergens may be helpful to clinical practice and basic research.

Cardiomyopathy, Dilated ; etiology ; pathology ; therapy ; Humans ; Myocardium ; pathology

AlM:To compare the results of lOL Master, contact and immersion A-scan ultrasound measurements for anterior chamber depth ( ACD ) , and evaluate the difference and consistency.METHODS:Fifty-eight cases (98 eyes) with age-related cataract during July to October in 2013 did the A-scan ultrasound with contact and immersion measurements and lOL Master to get the results of ACD. Difference in measurements between methods was assessed using the variance analysisi. Consistency was assessed using Bland-Altman.RESULTS:The ACD measured by lOL Master was 2. 31~3. 90mm, the mean was 3. 03 ± 0. 38mm. The ACD measured by contact A- scan ultrasound was 1. 51 ~4. 06mm, the mean was 2. 88 ± 0. 56mm. The ACD measured by immersion A-scan ultrasound was 1. 99 ~4. 17mm, the mean was 3. 17±0. 38mm. The results of lOL Master and contact A - scan ultrasound had statistical differences (P=0. 022<0. 05). The results of lOL Master and immersion A - scan ultrasound had statistical differences (P=0. 031<0. 05). The results of contact A-scan ultrasound and immersion A-scan ultrasound had statistical differences (P=0. 000<0. 05). The consistency between three methods was poor. CONCLUSlON: The rank of ACD of patients with cataract is immersion A-scan ultrasound, lOL Master and contact A-scan ultrasound. The consistency is poor, and the three methods can’t be interchanged clinically.

Cell Growth Processes ; Hemangioma ; pathology ; physiopathology ; Histiocytoma, Benign Fibrous ; pathology ; physiopathology ; Humans ; Neoplasms ; physiopathology

Objective To investigate the effect of Atorvastatin Combined Naoxintong Capsule treats to cerebral infarction and carotid atherosclerosis.Methods 134 patients with cerebral infarction and carotid atherosclerosis were diagnosed by MRI or CT scan.They were randomly divided into two groups.67 cases were given atorvastatin versus aspirin received routine treatment in the control group and 67 cases were used Naoxintong capsule in observation group on the basis of routine treatment in the observation group.The blood lipid,serum high sensitive C-reactive protein (hs-CRP) level,neural function defect score (NIHSS),carotid artery intima-media thickness (IMT) were comperred before and after treatment in two groups for treated with 1 courses (6 months).Results The blood lipid had no significant difference between two groups before and after treatment (P ＜ 0.05).The IMT,NIHSS score of high sensitivity C-reactive protein levels had no significant difference in two groups before and after treatment (P ＜ 0.05).The scores of NIHSS,IMT,high sensitive C-reactive protein level respectively (10.08 ± 1.82) ％,(1.06 ± 0.13)mm,(3.89 ± 1.41) mg/L of observation group were more decreased significantly than the control group (respectively (13.92 ±2.01)％,(1.24 ±0.15)mm,(5.02 ± 1.52) mg/L after treatment.Conclusion Atorvastatin Combined Naoxintong Capsule in treatment of cerebral infarction and carotid artery gruel kind hardening shows good therapeutic effect,it is worth for clinical use.

Objective To investigate the incidence of abnormal thyroid function in pregnant women and explore the significance of thyroid function screening in early pregnancy. Methods We randomly selected 630 cases of pregnant women who received routine inspection in the obstetric clinic in our hospital, and gave them five items of thyroid function screening besides routine check at the same time. Then we statistically analyzed the test results, and followed up 61 cases of pregnant women with hypothyroidism or subclinical thyroid function (hypothyroidism) to learn the treatment and adverse pregnancy outcomes,so as tto explore the correlation between hypothyroidism, subclinical hypothyroidism and adverse pregnancy outcomes. Results In 630 cases of pregnant women,there were 8 cases of hyperthyroidism (1.3%),61 cases of hypothyroidism or subclinical hypothyroidism (9.7%),including 5 cases of hypothyroidism (0.8%), 56 cases of subclinical hypothyroidism (8.9%) .There was statistically significant difference in TSH and FT4 between hypothyroidism group and normal control group ( <0.01) .There was statistically significant difference in TSH,FT4 and TT4 between subclinical hypothyroidism group and normal control group ( <0.05) . In 61 cases of hypothyroidism and subclinical hypothyroidism, 39 patients received levothyroxine therapy,22 received no treatment. The incidence of adverse pregnancy outcomes in the treatment group was significantly lower than the untreated group ( <0.05) . Conclusion Thyroid function testing in 6-20 weeks in early pregnancy has important significance in timely founding gestational thyroid dysfunction, so it should be as routine examination project.

Tea polyphenols (TPs), major biological active constituents of green tea, exert moderate and selective anticancer effects. Molecular mechanisms of TPs in cancer prevention and treatment involve multiple potential molecular targets. TPs inhibit growth factor receptor-mediated signal transduction pathway, decrease the activities of mitogen activated protein kinases and activator protein transcription factor-1, block nuclear factor-kappaB signaling pathway, reduce proteasome activity, lower overexpression of COX-2, subside dihydrofolate reductase and telomerase, and inhibit DNA methylation and matrix metalloproteinases. Furthermore, TPs enhance the inhibitory effect on the growth of cancers by traditional anticancer drugs or targeted antitumor drugs in vitro and in vivo and reverse multidrug resistances of cancer cells to vincristine, doxorubicin, and 5-fluorouracil. Besides, TPs reduce the nephrotoxicity induced by cisplatin, ameliorate irinotecan-induced side effects in the small intestine of mice, and decrease bleomycin-caused DNA damage in human leukocytes. TPs also increase antitumor activity of vaccine through immunological modulation. TPs play roles of the augmentation of antitumor effects, the reversal of multidrug resistance, and the reduction of side effects of chemotherapeutic drugs. TPs could be used as biochemical modulators in cancer therapy.

Allopurinol is a xanthine oxidase inhibitor,it can reduce production and excretion of uric acid and oxygen free radicals generation. Allopurinol has good therapeutic effects on gout and hyperuricemia,and can relief symptoms of prostatitis. In addition, allopurinol has protective effects on ischemical reperfusion injury,inflammation and tumor.

OBJECTIVE:To discuss the development prospect of the hospital pharmacists in clinical trials and to accumulate experience for the development of hospital pharmacy. METHODS: The current strengths,weakness,opportunities and threats of the hospital pharmacists were systematically analyzed and evaluated with the Porter's five strengths and PSET models;the development strategies were formulated by SWOT matrix. RESULTS: There were not only the apparent strengths and weakness but also the opportunities and threats for hospital pharmacists in clinical trials. CONCLUSION: At present,hospital pharmacists should take advantage of the opportunities of clinical trials market,stick to the aim of the professional development,spare no effort in discipline construction and training of professional talents and establish the space for their development and their indispensable position by giving priority to scientific research with service as target.

Objective To observe the effect of cupping plus Chinese medicinal fumigation on expectoration in patients with chronic obstructive pulmonary disease (COPD) due to phlegm-heat obstructing the lung. Method Eighty-two patients with COPD due to phlegm-heat obstructing the lung were randomized into a treatment group and a control group, 41 cases in each group. The control group was given regular Western medications, while the treatment group was intervened by cupping plus Chinese medicinal fumigation in addition to the treatment given to the control group, once a day, 5 d as a treatment course. The therapeutic efficacies were observed after 2 treatment courses. Result The clinical effective rate of the treatment group was higher than that of the control group, with a statistical significance (P<0.05). Conclusion Cupping plus Chinese medicinal fumigation can enhance the expectoration in patients with COPD due to phlegm-heat obstructing the lung.