1.Measurements of teeth, dental arch and base bone in Mongolia teenages
Zhen FU ; Weijia FENG ; Yong WANG
Journal of Practical Stomatology 2000;0(05):-
Objective: To obtain the data of dental measurements in healthy Mongolia teenages.Methods: Normal dentognathic models were obtained by cephalometric roentgenography in 56 teenages (30 boys and 26 girls) aged 10~17 years. The width of the tooth crown, the size of the dental arch and dental base bone were measured with a vernier. The measurments were statistically analysed.Results: The tooth crown in boys was wider than that in girls( P 0.05). There was no significant difference in Bolton index and Point index between boys and girls.Conclusion: The dental measurments may be refrences for dental clinic and research.
2.Hygienic Monitoring of Microbial Contamination of Filtrated Water and Edible Ice
Qing ZHEN ; Yong LI ; Shao WANG
Journal of Environment and Health 2007;0(08):-
Objective To know the status of microbial contamination in filtrated water and edible ice used in food processing,and to provide experimental basis for the management of HACCP of filtrated water and edible ice. Methods The samples of water and ice were collected from a western style restaurant in Changchun. The microbial indicators were tested based on Standard Examination Methods for Drinking Water(2001). Results Results In the third quarter, 37.8%, 32.4%, 13.5% of total bacterium counts, total coliform counts, feces coliform counts of the water samples was unqualified. Conclusion The filtrated water and edible ice used in restaurants can be contaminated by microbes in degree. It is necessary for HACCP of filtrated water involved coliform counts contamination to carry out dynamic monitoring.
4.Effect of high glucose on the expression of KLF6 in human lens epithelial cell
Hong-tao, WANG ; Yong-zhen, BAO
Chinese Journal of Experimental Ophthalmology 2012;30(2):136-140
Background Epithelial-mesenchymaltransition (EMT)isamajorcontributortothe pathogenesis of posterior capsular opacification(PCO).Kruppel-like factor 6 (KLF6) is a zinc finger protein,which can be stimulated by high glucose in proximal tubule cells and involved in transforming growth factor beta (TGF-β)induced EMT of diabetic nephropathy.ObjectiveThis study was designed to investigate the effect of high glucose on the expression of KLF6 and its target genes( TGFB1,TGFBR1,COLIA1,HSP47) in human lens epithelial cells (LECs).MethodsHuman LECs(SRA01/04) were cultured and exposed to different concentration of glucose.The expressions of KLF6 mRNA and protein were analyzed by real time polymerase chain reaction( real time PCR) and western blot after treatment with high glucose.The expressions of KLF6 target genes were analyzed by real time PCR to evaluate the EMT of SRA01/04 cells.ResultsCompared with the control group(5.5 mmol/L),the relative mRNA levels of t-KLF6 and wt-KLF6 in SRA01/04 treated with high glucose(22.2,44.4,66.6 mmol/L) increased obviously (F =72.53,42.02,P<0.01 ).Then,the concentration of 22.2 mmol/L was used in the next experiments.The relative mRNA levels of t-KLF6 and wt-KLF6 increased to the peaks after treatment with high glucose for 12 h,and began to decrease after 24 h until lower levels after 48 h ( F =100.12,125.52,P < 0.01 ).Western blot showed that the expression of KLF6 protein was also upregulated by high glucose treatment.With the promotion of the expression of KLF6 gene,the relative mRNA levels of TGFB1,TGFBR1,COLlAl and HSP47 of treated cells also respectively increased after treatment for 12 h,and began to decrease after 24 h until nearly at the levels of the control groups after 48 h( F=6.73,162.35,64.39,12.05,P<0.05 ).ConclusionsIt was concluded that high glucose induced the expression of KLF6 in human LECs,and KLF6 transiently stimulated the expression of target genes TGFB1,TGFBRl,COLlAl and HSP47 which were mainly involved in the mechanism of EMT.
5.Targeting angiogenesis and vascular remodeling as a novel therapeutic approach to liver fibrosis.
Xun-xun WU ; Cai-zhen ZHANG ; Xin WANG ; Yong DIAO
Acta Pharmaceutica Sinica 2015;50(5):535-540
Development of liver fibrosis is closely associated with angiogenesis and abnormal vascular remodeling. Recent studies have highlighted the importance of angiogenesis and vascular remodeling in fibrogenesis, the results that inhibition of angiogenesis is effective in suppression of liver fibrosis demonstrate that therapies with several molecular targets against angiogenesis, inflammation and fibrosis might be beneficial for the treatment of cirrhosis. However, there is some evidence that inhibition of angiogenesis can even worsen fibrosis. This article outlines recent advances regarding the interplay between inflammation, angiogenesis and fibrogenesis in terms of cellular and molecular mechanisms, and suggests a requirement of greater understanding to intervene in these key processes, such as liver sinusoidal endothelial cell fenestration and impact distinct chemokine actions driving monocyte migration and differentiation, for therapeutic benefit in the future.
Humans
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Inflammation
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therapy
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Liver Cirrhosis
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therapy
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Neovascularization, Pathologic
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therapy
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Vascular Remodeling
6.Prediction of walking and life span of children with cerebral palsy.
Zhen WANG ; Xiang-shu WEN ; Yong-ku LI
Chinese Journal of Pediatrics 2003;41(3):196-198
Cerebral Palsy
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mortality
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pathology
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physiopathology
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Child
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Humans
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Prognosis
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Survival Analysis
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Survival Rate
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Time Factors
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Walking
7.Two special cerebral hyponatremia.
Xiang-zhen NAN ; Yong-qing WANG ; Chun-xue YAN
Chinese Journal of Pediatrics 2003;41(9):715-717
8.Investigation of ethnic medicinal plants Orobanche, Cistanche and Boschniakia.
Zhen-Fang BAI ; Yong LIU ; Xiao-Qin WANG
China Journal of Chinese Materia Medica 2014;39(23):4548-4552
In this paper the species of ethnic medicinal plants Orobanche, Cistanche and Boschniakia, and their ethnopharmaceutical uses were comprehensively summarized by field investigation, systematical data analysis and comparison of relevant specimen and references. The results showed that six plants belonging to Orobanche were used as seven kinds of ethnic medicinal plants, two plants attributing Boschniakia were used as ten kinds of ethnic medicinal plants, two plants of Cistanche were used as three ethnic medicinal plants. The same plant was often used as different ethnic medicine in varied ethnic minorities. The effects of the ethnic medicines included yang-tonifying, hemostasis and analgesic activities. Hence, it is necessary to develop the rich plant resource of Orobanche for alleviation of Cistanche resources shortage.
Cistanche
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chemistry
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Drugs, Chinese Herbal
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chemistry
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pharmacology
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Humans
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Orobanchaceae
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chemistry
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Orobanche
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chemistry
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Phytotherapy
9.Effects of allogenic intra-bone marrow bone marrow transplantation on the hematopoiesis in mice
Yahong YUAN ; Yong WANG ; Zhen WENG ; Yan DING ; Dongsheng LI
Basic & Clinical Medicine 2010;30(1):19-23
Objective To investigate the effects of allogenic intra-bone marrow bone marrow transplantation (IBM-BMT) on re-establishing hematopoiesis in mice. Methods Bone marrow mononuclear cells (BMNCs) from BALB/ c mice were transplanted into the C57BL/6 mice treated with a lethal dose of ~(60)Coγ-ray radiation through intra-bone marrow injection or intravenous injection. Sixty of the C57BL/6 mice were randomly divided into three groups as higher dose intra-bone marrow injection group (IBM1 group), lower dose intra-bone marrow injection group (IBM2 group) and intravenous injection group (IV group). The nucleated cell numbers of whole bone marrow from the tibia of each recipient mouse were counted respectively at the day 1, day 3, day 6 and day 9 after the transplantation. The donor-derived total nucleated cells and myeloid cells were quantified by flow cytometry. Results At 6th day after transplantation, more total bone marrow nucleated cells, total donor-derived nucleated cells and donor-derived myeloid cells in the tibia of injected side in both IBM1 group and IBM2 group were found than that in IV group (P<0.05 or P<0.01). Conclusion Compared with traditional bone marrow transplantation (IV-BMT),IBM-BMT improves the bone marrow hematopoiesis in the early hematopoietic re-establishing stage in allogenic bone marrow transplantation.
10.Extraction of Myrrha by SFE-CO_2
Yong WANG ; Yan CHEN ; Xudong CHENG ; Zhen OUYANG ; Xiaobi JIA
Chinese Traditional Patent Medicine 1992;0(02):-
AIM: To optimize the extraction process of Myrrha by SFE-CO 2. METHODS: Orthogonal design was applied and GC was used to determine the contents of ?-Elemene in order to optimize the process. RESULTS: The optimized conditions were as follows: pressure 25 MPa, temperature 45 ?C and extraction time 4 h. CONCLUSION: The method of SFE-CO 2 is rapid, convenient in comparison with conventional methods.