1.SIMPLIFICATION URETEROSCOPE TREATMENT THE OBSTRUCT IN URETER (REPORT OF 768 CASES)
Xing ZHOU ; Chunxiao LIU ; Shaobo ZHEN
China Journal of Endoscopy 2001;7(1):10-11
Objective:To study and sum up the experience of diagnosis and treatment of ureter obstruct by Simplification ureteroscope.Methods:the patients of 750 cases for ureterolith underwent ureteroscopic lithotomy and pressune orbit lithotripsy by using caudal anesthesia in outpatient the 11 cases ureterostensis were treated by watery capus expand,ureteroscopic hard expand and ureterotectomy by using of self-made electrode.Then,transurethral electro incisions of ureterocles were carried out for 2 patients.Results:Successful rate of ureteroscopic lithotomy is 98.4%;12 patient of upper ureter stone received ESWL because of stone entering the renal pelvis,and 3 cases complicated ureteral perforation;11 cases ureterostensis and 7 cases ureterocele were cured.Conclusions:It is frist chosen for ureteroscope to ureteral stone,ureteral stricture,and ureteroceles with a diameter of ≤3cm,and the method have ideal cure effect and the patients will receive.
2.Application of Direct Gene Disruption Method in Recombinant Pichia pastoris
Zhen-Hua NI ; Xiang-Shan ZHOU ; Yuan-Xing ZHANG ;
Microbiology 1992;0(06):-
Proteolytic degradation has been a severe problem when Pichia pastoris is employed to express recombinant proteins.One alternative method to circumvent this problem is to construct protease gene disruptant.However,the main study of gene disruption is focused on nonrecombinant Pichia pastoris rather than recombinant strain.In our study,we established two different methods to directly disrupt PRC1 and KEX1 gene in recombinant Pichia pastoris.On the basis of this,we further discussed and compared the application and advantages of both methods.
3.Effect of Rehabilitation Medical Professional Training in Guangzhou, China
Zhou XING ; Zhitong LIN ; Cuihuan PAN ; Yan CHEN ; Rui YU ; Yu ZHOU ; Zhengmao YE ; Zhen FU
Chinese Journal of Rehabilitation Theory and Practice 2015;21(3):370-372
Objective To investigate the effect of rehabilitation professional training. Methods Based on the 10 tertiary hospitals in Guangzhou, Guangdong, the professionals from communitiy hospitals were trained. The scores of knowledge and practice tests were analyzed in 2 terms of training. Results The students performed better in the practice test (82.01±8.91) than knowledge (71.08±10.05) (P<0.01). The score of knowledge tests was better in the second term than the first (P<0.05), no difference between them in the score of practice test. Conclusion Rehabilitation professional training in Guangzhou can improve the ability of service of medical rehabilitation.
4.Effects of lamotrigine on cognitive function and quality of life in epilepsy patients
Pei-Min YU ; Guo-Xing ZHU ; Qi-Hao GUO ; Dong ZHOU ; Lie-Min ZHOU ; Ding DING ; Yan ZHOU ; Zhen HONG ;
Chinese Journal of Neurology 2005;0(09):-
Objective To explore the effects of lamotrigine on the cognitive function and the quality of life in epilepsy patients.Methods This was a prospective study and 91 newly diagnosed epilepsy patients were enrolled.The neuropsychological tests score and the quality of life in epilepsy inventory(QOLIE-31) were obtained before and after the treatment with lamotrigine.A battery of neuropsychological tests comprised the auditory verbal learning test(AVLT), the logical memory test(LMT), the digital symbol test(DST), the stroop color word test(SCWT), the trail making test(TMT), the verbal fluency test(VFT), the WAIS block design test(WBDT), the WAIS digital span test(WDST)and the Boston naming test(BNT). Results The repeated assessments in the patients taking lamotrigine were associated with significant improvements in many domains.The greatest changes were observed in the immediate and delayed recall of AVLT, DST, the time consuming of SCWT card C and TMT test A and B, the immediate and delayed recall of LMT, VFT, WBDT and BNT.For the quality of life, significant improvements were recorded in the fields of the seizure worry(38.81?16.06 vs 45.68?15.18), the overall quality of life(59.12?13.50 vs 64.99?13.33), the social function(64.59?25.14 vs 69.41?22.70)and the self-health evaluation (71.18?13.73 vs 76.75?11.30).Conclusion Improvements of the cognitive function and the quality of life can be observed in the initial period of medication with lamotrigine in epilepsy patients.
5.Clinical study of Reinhartdt and sea cucumber capsule combined with donepezil in treating Alzheimer's disease.
Zhi-Lin ZHOU ; Li-Zhen LIANG ; Yong-Xing YAN
Chinese Journal of Integrated Traditional and Western Medicine 2007;27(2):110-113
OBJECTIVETo study the efficacy and safety of Reinhartdt and sea cucumber capsule (RSC) combined with donepezil in treating Alzheimer's disease (AD), and its effect on thyroid function axis.
METHODSSixty-eight patients were randomly assigned to the RSC group, the Donepezil group and the combined treatment group, who were treated for 3 and 6 months with RSC, Donepezil and RSC combined with Donepezil, respectively. The curative effect was evaluated by scoring according to Mini-Mental State Examination (MMSE), ADAS-Cog and ADL chart, and the level of thyroid hormones, including TSH, FT3, FT4, TT3 and TT4, were measured with radioimmunoassay before treatment, 3 and 6 months after treatment respectively.
RESULTSAs compared with the baseline, MMSE score increased, ADAS-Cog score and ADL score decreased significantly in all the three groups after 3 months and 6 months of treatment (P < 0.05 and P < 0.01), but the improvement in the combined treatment group was more significant than that in the other two groups (P < 0.01). After 6 months of treatment, the levels of FT3 and FT4 in the combined treatment group were significantly changed (P < 0.01), but no significant change in all the thyroid hormones was found in the other two groups. No obvious adverse reaction occurred in all the three groups.
CONCLUSIONRSC combined with Donepezil in treating AD is effective and safe with no evident adverse reaction, better than single drug treatment, which may be through influencing the metabolism of thyroid hormones to improve the cognition function of AD patients.
Aged ; Aged, 80 and over ; Alzheimer Disease ; drug therapy ; Animals ; Capsules ; Drug Therapy, Combination ; Female ; Humans ; Indans ; therapeutic use ; Male ; Medicine, Chinese Traditional ; Middle Aged ; Nootropic Agents ; therapeutic use ; Piperidines ; therapeutic use ; Radioimmunoassay ; Sea Cucumbers ; chemistry ; Thyroid Hormones ; blood
6.Advances in Research on the Mechanisms of Saccharomyces cerevisiae Ethanol Tolerance
Sui-Sheng ZHANG ; Ri-Bo HUANG ; Xing ZHOU ; Zhen-Chong LI ; Zhi-Min HUANG ;
Microbiology 2008;0(10):-
The yeast Sacchromyces cerevisiae is most widely used for producing bioethanol in alcoholic industry due to its higher ethanol yield and fermentation rate. However, the toxic effect of accumulated ethanol is one of the main factors, which limit high ethanol production. Thus, investigating the mechanisms of yeast ethanol tolerance will provide the basis for solving the industrial problem. This article reviewed the mechanisms of Sacchromyces cerevisiae ethanol tolerance focusing on its cell physiological behaviors, structure and biochemical composition, as well as its genetic basis.
7.Solitary Fibrous Tumor of the Kidney Treated with Laparoscopic Partial Nephrectomy: A Case Report.
Xing CHEN ; Jian-Feng WANG ; Zhen-Shan DING ; Xiao-Feng ZHOU
Chinese Medical Sciences Journal 2018;33(1):64-68
We herein reported a 27-year-old woman with a right renal mass for two years. She underwent laparoscopic partial nephrectomy. Immunohistochemical examination of the specimen confirmed the diagnosis of solitary fibrous tumor by revealing its positive staining for cluster of differentiation (CD)34, epithelial membrane antigen (EMA), B-cell lymphoma-2 (Bcl-2) and CD99 in the tumor cells. No adjuvant treatment was carried out. The patient was in good health without local recurrence or metastasis during 2 years of follow-up. Laparoscopic partial nephrectomy for renal solitary fibrous tumor is an alternative treatment to radical nephrectomy. It can provide a good outcome. However, further follow-up and more cases of renal solitary fibrous tumor treated with laparoscopic partial nephrectomy are necessary to compare the oncological outcome with radical nephrectomy.
8.Study on hepatotoxicity of aqueous extracts of Polygonum multiflorum in rats after 28-day oral administration: cholestasis-related mechanism.
Tao WANG ; Jia-ying WANG ; Zhi-xing ZHOU ; Zhen-zhou JIANG ; Yan-yan LI ; Liang ZHANG ; Lu-yong ZHANG
China Journal of Chinese Materia Medica 2015;40(11):2163-2167
OBJECTIVETo study the effect of aqueous extracts of Polygonum multiflorum (AEPM) on bile acid synthesis, metabolism and transfer-related molecules in rat liver and the hepatotoxicity-related mechanism of P. multiflorum.
METHODSprague-Dawley rats were orally administered with 30, 60 g x kg(-1) APEM once everyday for consecutively 28 days. At the end of the experiment, mRNA and protein expressions of hepatic MRP3, MRP2, BSEP, FXR and CYP7A1 were detected by Real-time PCR and Western blot
RESULTCompared with the normal group, the AEPM high dose group showed significant increases in mRNA expressions of hepatic MRP3 and BSEP of male rats (P < 0.05); AEPM high and low dose groups revealed a notable decrease in mRNA expressions of hepatic FXR (P < 0.05) and remarkable rises in mRNA expressions of hepatic MRP3, MRP2, BSEP, CYP7A1 among female rats (P < 0.05). According to the test results of western blot assay, AEPM high and low dose groups showed consistent changes in protein and mRNA expressions hepatic MRP3, MRP2, BSEP, FXR, CYP7A1.
CONCLUSIONThe 28 oral administration with AEPM in rats showed a certain effect on expressions of bile acid synthesis, metabolism and transfer-related proteins, as well as cholestatic or choleretic effects in the mRNA expression.
Administration, Oral ; Animals ; Bile Acids and Salts ; metabolism ; Cholestasis ; chemically induced ; Fallopia multiflora ; Female ; Liver ; drug effects ; Male ; Plant Extracts ; toxicity ; Rats ; Rats, Sprague-Dawley
9.Clinicopathological features and molecular genetic analysis of endolymphatic sac tumor: report of 2 cases.
Qiu RAO ; Xiao-jun ZHOU ; Xing-zao JIN ; Heng-hui MA ; Hang-bo ZHOU ; Zhen-feng LU
Chinese Journal of Pathology 2010;39(6):412-413
Adenoma
;
metabolism
;
pathology
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Adult
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Carcinoma, Renal Cell
;
metabolism
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pathology
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Diagnosis, Differential
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Ear Neoplasms
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complications
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genetics
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metabolism
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pathology
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surgery
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Endolymphatic Sac
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Female
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Follow-Up Studies
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Humans
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Kidney Neoplasms
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metabolism
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pathology
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Middle Aged
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Paraganglioma
;
metabolism
;
pathology
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Point Mutation
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Von Hippel-Lindau Tumor Suppressor Protein
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genetics
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von Hippel-Lindau Disease
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complications
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genetics
;
metabolism
10.The virological response in prolonged therapy of chronic hepatitis C with low-dose peginterferon alpha-2a
Jun-Ying ZHOU ; Dong-Fang ZHOU ; Wen-Li XING ; Cai-Yan ZHAO ; Zhen ZHEN
Chinese Journal of Experimental and Clinical Virology 2010;24(2):142-144
Objective To investigate the virological response in prolonged therapy of chronic hepatitis C (CHC) with low-dose peginterferon alpha-2a.Methods The 92 cases of in-patients with chronic hepatitis C in September 2004 to September 2006 were divided to three groups according the endurance of interferon.The dose of peginterferon alpha-2a was 67.5 μg,90 μg and 180 μg per week in group A,B and C respectively.The treatment duration of peginterferon alpha-2a was 96 or 48 weeks in HCV genotype 1b and 2a in group A and B,and in the group C the duration was 48 or 24 weeks in genotype 1b and 2a patients respectively.Meanwhile,ribavirin for 900-1200 mg per day combined treated with all patients.The quantitation of serum HCV RNA were conducted to determine the rapid virological response (RVR),early virological response (EVR) and sustained virological response (SVR) respectively.Results There were no significant difference between the three groups in the rate of RVR,EVR and SVR(P>0.05).There was a higer rate of RVR,EVR and SVR in the genotype 2a group than the genotype 1b group (P<0.05).HCV genotype was the independent predictor(OR=12.78.95%,CI=11.97-82.89,P=0.0075) of SVR.Conclusion There was a similar virological response between prolonged therapy of chronic hepatitis C with low-dose peginterferon alpha-2a and the standard dose and duration.The genotype was the independent predictors of SVR in peginterferon alpha-2a antiviral therapy of chronic hepatitis C.