Metastasis-associated in colon cancer-1 (MACC1),a newly discovered gene which controls tumor growth and metastasis,abnormally expresses in a variety of malignant tumors.As a key regulator of HGF-MET signal pathway,its coding protein can obviously promote invasion and metastasis of tumor cells.

Animals ; Antioxidants ; pharmacology ; Ascorbic Acid ; pharmacology ; Cumulative Trauma Disorders ; metabolism ; Lipid Peroxidation ; drug effects ; Male ; Muscle, Skeletal ; injuries ; pathology ; Oxidation-Reduction ; Oxidative Stress ; drug effects ; Rats

Case-Control Studies ; Child, Preschool ; Congenital Hypothyroidism ; DNA ; genetics ; Female ; Humans ; Hypothyroidism ; genetics ; Infant ; Iodine ; metabolism ; Male ; Mutation ; Polymerase Chain Reaction ; Sodium ; metabolism ; Symporters ; genetics

OBJECTIVETo compare the cleaning effect of automatic cleaning machine and manual cleaning on coupling type surgical instruments.

METHODSA total of 32 cleaned medical instruments were randomly sampled from medical institutions in Putuo District medical institutions disinfection supply center. Hygiena System SUREII ATP was used to monitor the ATP value, and the cleaning effect was evaluated.

RESULTSThe surface ATP values of the medical instrument of manual cleaning were higher than that of the automatic cleaning machine.

CONCLUSIONCoupling type surgical instruments has better cleaning effect of automatic cleaning machine before disinfection, the application is recommended.

No abstract available.

Objective To explore the effect of conservative treatment for Stanford B aortic intramural hematoma without calcified plaque and the midterm follow-ups.Methods Clinical data of 20 patients with Stanford B aortic intramural hematoma without calcified plaque which were confirmed by CTA of the whole aorta were retrospectively analyzed.There were 12 males and 8 females,with the age of 35～ 52 years.All of them received pharmacotherapy and psychotherapy.Results All the 20 cases were asymptomatic after conservative medical treatment when they discharged,and were followed up whit imaging tests.They all lived without symptoms during the follow-up period.The pleural fluid on the left side was disappeared in 11 cases after 3 months and the aortic intramural hematoma was absorbed in 13 cases after 6 months.After 1 year there were total 18 cases without aortic intramural hematoma,while The thickness and scope of the hematoma were reduced significantly in the other 2 cases.Conclusion It' s ease to find whether the patient with Stanford B aortic intramural hematoma got calcified plaque or not; the conservative treatment for Stanford B aortic intramural hematoma without calcified plaque has satisfactory clinical effect in midterm follow-ups.

Objective To examine the expression of metastasis-associated in colon cancer 1 (MACC1) gene in ovarian cancer cell lines and investigate its effect on biological behaviors of ovarian cancer cells.Methods The expression of MACC1 was examined by qRT-PCR and Western blot analysis in four ovarian cancer cell lines inculding OVCAR3,ES-2,SKOV3 and HO-8910.When the MACC1 was transfected to OVCAR3 cells,fluorogenic quantitative PCR was used to filter and identify MACC1 gene after the efficient silencing.Changes of adhesion in the cells were analyzed by an adhesion assay.Transwell migration and invasion assay and in vitro vascular mimicry assay were used to detect migration,invasion and angiogenesis of OVCAR3 cells in vitro.Results The expression of MACC1 gene was higher in OVCAR3 compared to other cell lines.qRT-PCR confirmed that the expression of MACC1 was silenced successfully after transient transfected MACC1-siRNA into OVCAR3 cells.After successful silencing the MACC1 expression,the adhesion ability was inhibited to some degree.In transwell migration assay,the numbers of cells in upper chamber passing through the membrane in transfected group were less than control groups (245.5 ±12.8,500.3±16.5 and 496.3±13.1 respectively),while in transwell invasion assay,the numbers of cells in upper chamber passing through the membrane in transfected group were less than the negative group and control group (185.3±14.1,405.7±9.1 and 416.3±11.5 respectively),both with markedly differences among the three groups.In tube formation assay,the distrubition of HUVECs was diffused with less junctions,and the average number of complete tubular structure was decreased in transfected group compared to the corresponding controls.Conclusion RNA interference inhibits the expression of MACC1 and effectively inhibits the metastasis and invasion abilities of ovarian cancer cells in vitro,and MACC1 is expected to become the target gene of ovarian cancer treatment.

Objective To evaluate covered stent placement in the treatment of iliac artery rupture in the process of iliac artery angioplasty.Methods Clinical data of 9 patients' iliac artery rupture in the process of iliac artery angioplasty were retrospectively reviewed.Results Covered stent was successfully placed in all cases.The patency of involved iliac artery was identified in 8 cases by angiography and there was no contrast extravasation.Type Ⅰ endoleak occurred in one patient in the proximal end of the stent graft and iliac artery graft replacement was done by open surgery immediately.Postoperatively clinical symptoms were relieved in all these 9 patients.One case lost to follow up.8 patients were followed up for 14 to 45 months,average (22 ±9) months.Stenosis on the distal end of the covered stent (external iliac artery)was found in one patient who underwent external iliac artery balloon-expandable stent angioplasty.One patient with artificial vessel placement was symptoms free with patency of the involved artery as reviewed by ultrasonography on 18 month followed up.Conclusions When iliac artery rupture in the process of iliac artery angioplasty should develop,the first choice is immediately covered stent placement therapy,while open surgery remains as the last resort.

Objective To investigate the suitable therapeutic schedule for Stanford B aortic intramural hematoma associated with calcification. Methods During the period from March 2009 to March 2012, a total of 15 patients of Stanford B aortic intramural hematoma with calcified plaque were admitted to authors’ hospital. The diagnosis was proved in all patients by CT angiography of the entire aorta with a 64-row CT scanner. Of the 15 patients, death occurred in one, thoracic endovascular aortic repair (TEVAR) treatment was adopted in 7 and conservative therapy was carried out in 7. All the patients were followed up for one year. CT angiography was employed at 3, 6 and 12 months after the treatment to evaluate the therapeutic results. Results One patient died of acute myocardial infarction after admission to hospital when the long-tem use of antiplatelet drugs was stopped. Seven patients received TEVAR treatment and the remaining 7 patients were treated with strict conservative therapy, and all these patients were asymptomatic at the time of discharge. During the follow- up period, CT angiography performed at 3, 6 and 12 months after the treatment showed that the intramural hematoma lesions gradually shrank or were absorbed in 13 patients, and the patients were asymptomatic. The remaining one patient was lost in touch. Conclusion The key to treat Stanford B aortic intramural hematoma with calcification is to prevent deterioration of the lesion. While strict conservative treatment is kept on, TEVAR should be promptly carried out for patients who need to take antiplatelet drugs over a long period of time, for patients whose clinical symptoms are not improved, for patients in whom the relived symptoms recur, and for patients whose CT angiography shows that the penetrating aortic ulcer becomes worse.

Objective To study the adverse effect of house indoor air pollution on children respiratory system health in Lanzhou city.Methods A retrospective questionnaire investigation on 1 700 children respiratory health was carried out in three kindergartens,three elementary schools involving lower grade pupils in three districts in Lanzhou city.At the same time,the information about the home environment of the children and family background was collected.Applying logistic regression model, the influencing factors were analyzed.Results The over-standard rates of indoor air quality were from 12.4% to 43.8% in Lanzhou.In the non-heating period,the over-standard rates of formaldehyde,benzene,SO_2,NO_2,CO and PM10 in indoor air were 22.6%,14.5%,13.3%,12.4%,0% and 23.3% respectively.In the heating period,the over-standard rates of the pollutants were 27.3%,16.4%,34.6%,28.5%,32.3% and 43.8% respectively.Indoor air pollution was statistically associated with children respiratory symptoms and diseases,the related risk factors were someone smoking in home,house decorated,opening windows, ventilation,heating in winter,sharing bedroom and maternal education.The outdoor air pollution was one of the influencing factors. Conclusion Children respiratory diseases and symptoms are related to many air pollution factors,such as outdoor and indoor air pollution,personal characteristic and family factors,so to prevent and control indoor air pollution,a comprehensive way should be taken in Lanzhou city.