1.Status on knowledge, attitudes, behavior regarding nutrition and food safety among 4-6 grade students from Chinas' two poverty-stricken counties
Zhen PENG ; Fang YANG ; Zhen-Qiu SUN ; Ming-Zhi LI
Chinese Journal of Epidemiology 2013;34(7):673-676
Objective To investigating knowledge,attitudes,current behavior among primary school students on nutrition and food safety in two State-level poverty-stricken counties,and to explore the best educational model on nutrition and food safety.Methods 478 pupils at 4-6 grades were selected,using the method of stratified cluster sampling and questionnairs.Results The average score on knowledge was 18.66 ± 3.75,with the qualified rate as 61.09% (the highest was 60.6%).The average score in the section of attitude was 5.82 ± 1.50.More than 97% of the students agreed that it was necessary to receive knowledge on nutrition and food safety.The average score of behavior was 8.52 ± 2.56.Conclusion Rate of awareness on knowledge of nutrition and food safety had greatly improved among the students under study.Their habit tended to be more regular.However,we also noticed the gap between knowledge and behavior on this issue.
2.3D computed tomographic analysis of frontal recess region.
Peng WANG ; Yanhong ZHANG ; Xiaodong HAN ; Gui YANG ; Jianping LAN ; Hailiang ZHAO ; Shuqi QIU ; Hongtao ZHEN ; Qixue GAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(5):311-317
OBJECTIVE:
The purpose of the study was to observe the three-dimensional (3D) CT imaging features of the frontal recess region with 3D reconstruction, and obtain the real image of the important anatomical structures of the region to conduct surgery.
METHOD:
Five patients were undergone spiral CT by 16 line high speed spiral CT, and multiplanar reconstruction images using standard 3D reconstruction protocol on a computer workstation. The structure of the frontal recess, the agger nasi cell and adhere style of the uncinate process were observed. The parameter of the important anatomic structure of frontal recess was measured precisely.
RESULT:
After the reconstruction, we get the 3D model very close to the true state of the nasal cavity--sinuses cell, in which parts of the frontal recess can clearly identify the agger nasi cell, frontal cell and other important structures. In this patient, the height, width and depth of the agger nasi and frontal sinus were 12.3 mm, 12.1 mm, 38.5 mm, respectively.
CONCLUSION
This study tried to develop the standardized techniques and measurements from 3D reconstructed images of the frontal sinus and to ascertain the usefulness of the frontal sinus in identification of patients. The results in better preoperative patient counselling and in predicting postoperative improvement in clinical status.
Adolescent
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Adult
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Aged
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Female
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Frontal Sinus
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diagnostic imaging
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Humans
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Imaging, Three-Dimensional
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Male
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Middle Aged
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Tomography, Spiral Computed
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Young Adult
3.Computed tomographic images analysis of frontal recess anatomy based on three-dimension reconstruction.
Peng WANG ; Xiaodong HAN ; Gui YANG ; Yanhong ZHANG ; Jianping LAN ; Hailiang ZHAO ; Shuqi QIU ; Hongtao ZHEN ; Qixue GAO
Journal of Clinical Otorhinolaryngology Head and Neck Surgery 2014;28(17):1305-1310
OBJECTIVE:
To observe the CT three-dimensional imaging features of the frontal recess region with advanced three-dimensional reconstruction, and develop the real image of the important anatomical structures around the region to conduct surgery.
METHOD:
Thirty patients were undergone spiral CT by 16 line high speed spiral CT, and multiplanar reconstruction images using standard three-dimensional reconstruction protocol on a computer workstation. The structure of the frontal recess, the agger nasi cell and adhere style of the uncinate process were observed. The parameter of the important anatomic structure of frontal recess was measured precisely.
RESULT:
After the reconstruction, we get the three-dimensional model very close to the true state of the nasal cavity-sinuses cell, in which parts of the frontal recess can clearly identify the agger nasi cell, frontal cell and other important structures. In these patients, the height, width and depth of the agger nasi and frontal sinus were (9.45 ± 3.60)mm, (8.08 ± 3.37)mm, (26.98 ± 6.82)mm and (26.86 ± 9.45)mm, respectively.
CONCLUSION
This study tried to develop the standardized techniques and measurements from three-dimensional reconstructed images of the frontal sinus and to ascertain the usefulness of the frontal sinus in identification of patients. The project results in better preoperative patient counselling and in predicting postoperative improvement in clinical status.
Ethmoid Sinus
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diagnostic imaging
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Frontal Sinus
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diagnostic imaging
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Humans
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Image Processing, Computer-Assisted
;
methods
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Imaging, Three-Dimensional
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Nasal Cavity
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Paranasal Sinuses
;
diagnostic imaging
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Tomography, Spiral Computed
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methods
4.Distribution characteristics of methicillin-resistant Staphylococcus aureus in a children's hospital
ping Xiu ZHANG ; peng Hai LIU ; Qun GAO ; Wei CUI ; zhen Guang XU ; yue Qiu XU ; fang Qiong WU
Chinese Journal of Infection Control 2017;16(10):949-952
Objective To investigate the distribution characteristics of methicillin-resistant Staphylococcus aureus (MRSA)in a children's hospital,and provide basis for the prevention and control of MRSA infection in children. Methods Children who admitted to a children's hospital from 2011 to 2015 were analyzed retrospectively,clinical data of children,isolation of pathogens,types of specimens,and healthcare-associated infection(HAI)status were analyzed.Results From 2011 to 2015,a total of 911 children isolated Staphylococcus aureus (SA,1108 positive specimens),494 of whom isolated MRSA (599 positive specimens),54.23% of children isolated MRSA(isolation rate of specimens was 54.06%);there was no significant difference in the isolation rate of MRSA between children of different genders(P > 0.05);isolation rate of MRSA in different age groups was statistically significant(P <0.05).Isolation rates of MRSA from blood,puncture fluid,secretion,and pus were 68.97%,66.00%,55.81%, and 54.47% respectively.Isolation rate of SA and MRSA increased from 0.61% and 21.74% in 2011 to 1.40%and 75.59% in 2015 respectively,difference were both significant(both P <0.05).Incidence of SA and MRSA in-creased from 0.198% in 2011 to 2.697% and 2.119% in 2015 respectively,both showed an upward trend year by year(both P <0.05).Conclusion Isolation rate of MRSA and incidence of HAI in this children's hospital increased year by year,it is necessary to intensify management,use antimicrobial agents scientifically and rationally,timelyperform disinfection and isolation,so as to curb the emergence and spread of MRSA in hospital settings.
5.Effects of anisodamine on the expressions of vascular endothelial growth factor and intercellular adhesion molecule 1 in experimental infusion phlebitis.
Zhen-Xiang ZHANG ; Peng WANG ; Qiu-Shi ZHANG ; Xue PAN ; Qing-Xia ZHAO ; Xiao-Kai WANG
Chinese Medical Journal 2012;125(2):300-305
BACKGROUNDInfusion phlebitis is the most common side effect of clinical intravenous drug therapy and several clinical studies have demonstrated that anisodamine can effectively prevent the occurrence of infusion phlebitis. This study was designed to investigate effects of anisodamine on the expressions of vascular endothelial growth factor (VEGF) and intercellular adhesion molecule 1 (ICAM-1) in a rabbit model of infusion phlebitis and to analyze the mechanisms of anisodamine effect on the prevention and treatment of experimental infusion phlebitis.
METHODSTwenty-four specific pathogen-free male Japanese white rabbits were randomly assigned to the control group, the model group, the magnesium sulfate group and the anisodamine group. The rabbit model of infusion phlebitis, induced by intravenous administration, was established and expressions of VEGF and ICAM-1 were determined and contrasted with the control group treated with normal saline. We evaluated expression by histopathology, immunohistochemistry, reverse transcription-polymerase chain reaction, and Western blotting assay.
RESULTSPathohistological changes of the model group were observed, such as loss of venous endothelial cells, inflammatory cell infiltration, edema and thrombus. The magnesium sulfate group and the anisodamine group showed significant protective effects on vascular congestion, inflammatory cell infiltration, proliferation, swelling of endothelium and perivascular hemorrhage. The model group showed the highest expressions of VEGF and ICAM-1 of the four groups (P < 0.01). On the contrary, anisodamine alleviated the inflammatory damage by significantly reducing the expressions of VEGF and ICAM-1 compared with the model group (P < 0.01). There was no significant difference in the expressions of VEGF and ICAM-1 between the magnesium sulfate group and the anisodamine group (P > 0.05).
CONCLUSIONAnisodamine alleviates inflammatory damage by significantly reducing the expressions of VEGF and ICAM-1, and shows significant protective effects in an animal model of infusion phlebitis.
Animals ; Blotting, Western ; Immunohistochemistry ; Intercellular Adhesion Molecule-1 ; metabolism ; Male ; Phlebitis ; drug therapy ; Rabbits ; Random Allocation ; Reverse Transcriptase Polymerase Chain Reaction ; Solanaceous Alkaloids ; therapeutic use ; Vascular Endothelial Growth Factor A ; metabolism
6.Development of the quality of life scale specific for patients with benign prostatic hyperplasia.
Jing-cheng SHI ; Tai-sheng CAI ; Min-ning PENG ; Zhen-qiu SUN
Journal of Central South University(Medical Sciences) 2005;30(1):28-31
OBJECTIVE:
To develop a specific quality of life (QOL) scale for Chinese patients with benign prostatic hyperplasia (BPH).
METHODS:
The scale was developed with the programmed decision methods. The item pool was certified by experts. Five methods were used in item selection after a pilot study for which 256 BPH patients had been recruited. The scale was evaluated by its reliability and validity.
RESULTS:
We formed a 27-item quality of life scale specific for patients with benign prostatic hyperplasia prior test version (BPHSQL). The test-retest correlation coefficient and Cronbach's alpha coefficient of BPHSQL were 0. 774 and 0. 945. The structure of the scale was similar to the theory construction. The scale's correlation coefficients with criteria ranged from 0.531 to 0.700. BPHSQL could well discriminate the quality of life between BPH and non-BPH patients as well as patients with different degrees of symptoms, different sources and patients with or without urethral catheters.
CONCLUSION
BPHSQL is reliable, valid and sensitive, and will be a convenient tool in clinical research to provide advice on different treatments for different patients.
Adult
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Evaluation Studies as Topic
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Factor Analysis, Statistical
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Humans
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Male
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Prostatic Hyperplasia
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psychology
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Psychometrics
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Quality of Life
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Reproducibility of Results
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Surveys and Questionnaires
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standards
7.Video-electroencephalogram monitoring in children with nonconvulsive status epilepticus
Yun-Jian ZHANG ; Peng-Ling QIU ; Shui-Zhen ZHOU ; Yi WANG ; Dao-Kai SUN
Chinese Journal of Applied Clinical Pediatrics 2013;28(9):708-711
Objective To explore the clinical features and video-electroencephalogram (VEEG) monitoring of nonconvulsive status epilepticus (NCSE) in children.Methods 1.Object of study:Seventeen patients of NCSE diagnosed with Kaplan's criteria were analyzed in Children's Hospital of Fudan University between Oct.2009 and Sep.2012.2.Data analysis:Data on demographics,etiology,clinical manifestation and response to clonazepam therapy were analyzed.3.Therapies:Clonazepam 0.05 mg/kg was intravenously injected twice a day.Treatment of poor efficacy patients was combined with other antiepileptic drugs.4.Therapeutic effect:Clinical assessment of cognitive improvement and VEEG monitoring of background activity or paroxysmal abnormalities were analyzed.Results Nine male and 8 female of 17 patients with NCSE were involved,from 11 months to ll.4-year old.The clinical attacks lasted ranging variously time from 4 hours to 3 months.Each patient had a prolonged change of consciousness,accompanied by psychological or behavioral changes.Definite medical causes were identified in 65% (11/17 cases) of the patients.Secondary epilepsy was the dominating cause.The characteristics of ictal VEEG in NCSE generally included slow activity and focal or generalized δ or θ activity.After clonazepam treatment,the conditions of 13 patients were under complete control,in which 4 had improvement.Six cases of unknown cause were fully controlled within 72 hours after intravenous injection of clonazepam.The prognosis of CNS infection sequelae patients,metabolism disorders and brain structural damage was poor.Conclusions NCSE may present with confusion,behavioral disturbances and psychiatric conditions.The diagnosis can be made by the ictal and interictal VEEG monitoring.It is necessary to make the diagnosis and control the seizures as quickly as possible.Clonazepam is useful in NCSE.
8.Effectiveness on the education and food safety programs among senior primary students in 2 counties from west China-a cluster randomized trial
Min-Xue SHEN ; Zhen PENG ; Jing-Xuan HU ; Zhen-Qiu SUN ; Na ZENG ; Ming-Zhi LI
Chinese Journal of Epidemiology 2013;34(9):879-883
Objective To evaluate the effectiveness of nutrition and food safety educational programs on primary students from grade 4 to 6 in impoverished areas of Western China,under a school-based cluster-randomized trial.Methods Twelve primary schools were selected from 2 impoverished counties in West China and assigned to intervention or control groups,randomly,with 6 schools in each group.Self-rating knowledge,attitude and practice questionnaires were used at both baseline and final stages.Textbooks and supportive materials were designed according to the characteristics of baseline data.All students in the intervention group were treated with targeted nutrition and food safety lectures of 0.5 hour per week for 2 semesters.Generalized linear mixedeffects model was applied to fit the random effects on individual and clusters as well as to fit the fixed effect of the programs.Results Three hundred seventy-eight students from grade 4 to 6 were randomly selected at the baseline study and the differences of scores on knowledge,attitude and practice between the 2 groups were statistically insignificant (P>0.05).No cluster was lost during the trial.In the final investigation,478 students were randomly selected at the individual level.Scores on knowledge,attitude and practice among students in the intervention group were significantly higher,when comparing to the control group (P<0.01).At the cluster level,more schools in the intervention group showed significant changes on knowledge and practice,yet the change in attitude was less obvious.Data from the mixed-effects model demonstrated that the program served as an influential factor on scores related to knowledge after the intervention (P=0.015) but did not affect the scores on related attitude or practice (P>0.05).Conclusion Lectures seemed to have improved the cognition of nutrition and food safety among primary students from grade 4 to 6.However,long-term observation and larger sample size were needed to evaluate the changes on attitude and practice among the students.
9.Bioluminescent imaging monitoring of a anti-angiogenesis therapeutic gene vasostatin in tumor cell PC3.
Jie-miao HU ; Fei-chan QIU ; Bin YIN ; Yan-hua GONG ; Jian-gang YUAN ; Bo-qin QIANG ; Shi-zhen WANG ; Xiao-zhong PENG
Acta Academiae Medicinae Sinicae 2007;29(3):312-317
OBJECTIVETo generate a sensitive tool for noninvasive monitoring of a therapeutic gene vasostatin.
METHODSWe fused the bioluminescent reporter gene firefly luciferase to the therapeutic transgene vasostatin and ensured that these two proteins would not interrupt each other and kept their own natural character.
RESULTSWe therefore examined clones of PC3 cells stably expressing fusion gene and positive controlfluc with bioluminescence. In vivo imaging of PC3-Fluc subcutaneous tumors showed that the mean tumor bioluminescence increased in animals over several weeks.
CONCLUSIONNoninvasive monitoring facilitates the detection of gene expression in vivo and in vitro.
Animals ; Calreticulin ; genetics ; metabolism ; Cell Line, Tumor ; Gene Transfer Techniques ; Genes, Reporter ; Humans ; Luciferases, Firefly ; genetics ; metabolism ; Luminescent Measurements ; Neoplasm Transplantation ; Peptide Fragments ; genetics ; metabolism ; Recombinant Fusion Proteins ; genetics ; metabolism
10.Transfection with 5-lipoxygenase/green fluorescence protein for evaluating injury-induced 5-lipoxygenase translocation to the nuclear membrane in PC12 cells.
Yu-Mei YUAN ; Cheng-Tan LI ; Zhen-Qiu MA ; Fang PENG ; Wei-Ping ZHANG ; Yue-Liang SHEN ; Er-Qing WEI
Journal of Zhejiang University. Medical sciences 2007;36(2):111-116
OBJECTIVETo evaluate the translocation of 5-lipoxygenase (5-LOX)) after injuries by transfection with green fluorescence protein (GFP)/5-LOX in PC12 cells.
METHODSPC12 cells were stably transfected with pEGFP-C2/5-LOX (GFP/5-LOX) or pEGFP-C2 vectors (control). After treatment with oxygen-glucose deprivation (OGD), H(2)O(2) or NMDA, GFP/5-LOX localization in the cells was observed under a fluorescence microscope. Wild-type 5-LOX was determined by immunostaining after the treatment.
RESULTIn the GFP/5-LOX-transfected cells, GFP/5-LOX was primarily localized in the nucleus; while in the GFP-transfected cells, GFP was localized in both the cytoplasm and nucleus. After OGD and H(2)O(2) treatments, GFP/5-LOX was translocated to the nuclear membrane in 50.6 % and 57.7% cells respectively. However, after NMDA treatment or in GFP-transfected cells, no translocation was observed. Wild-type 5-LOX was distributed in the nuclei and cytoplasm, and all the 3 treatments induced 5-LOX translocation to the nuclear membrane.
CONCLUSIONIn the PC12 cells stably transfected with GFP/5-LOX, GFP/5-LOX is primarily distributed in the nuclei; the OGD-, H(2)O(2)- and NMDA-induced 5-LOX translocation exhibits different properties.
Animals ; Arachidonate 5-Lipoxygenase ; genetics ; metabolism ; Cell Nucleus ; metabolism ; Glucose ; pharmacology ; Green Fluorescent Proteins ; genetics ; metabolism ; Hydrogen Peroxide ; pharmacology ; Microscopy, Fluorescence ; N-Methylaspartate ; pharmacology ; Nuclear Envelope ; metabolism ; PC12 Cells ; Protein Transport ; drug effects ; Rats ; Recombinant Fusion Proteins ; genetics ; metabolism ; Transfection