2.Verification of colon-targeted release of Changankang Pellets
Huijun YAN ; Zhixian LONG ; Yurong WANG ; Li ZHEN ; Weihua GAO
Chinese Traditional Patent Medicine 1992;0(07):-
Objective:To determine the in vitro release of two kinds of Changankang Pellets (Radix Astragall, Rhizama coptid) coated with Surelease and Eudragit respectively and verify their colon-targeted release in vivo through X-ray photography. Methods: Changankang Pellets were made by mixing the medical BaSO 4 powder and appropriate drugs together according to original produce and coat methods. After determining their in vitro release, they were tested in vivo by 2 volunteers. X-ray films were taken after appropriate time and analyzed to determine the location and status of these pellets inside healthy objects. Results: Pellets made by this way can carry drugs through stomach, intestine and targeted-release them in colon; the coating combined with pH-dependent and time-dependent Eudragit thin layer has better results. Conclusion: X-ray radiography applying BaSO 4 powder could be taken as a quality control method for colon-targeted release drugs. The preliminary results showed that Changankang Pellets met the requirements of colon-targeted release.
3.Experimental research into the effects of Yumian Decoction anti-asthma formula(DYA) on the plasma cAMP、cGMP and PAF contents in asthmatic guinea pigs
Chu LI ; Zijiang LONG ; Zhen WANG ; Baocheng HU ;
Chinese Traditional Patent Medicine 1992;0(05):-
Objective: To investigate the anti asthmatic effects and its mechanism of Yumian Anti asthma Formula (DYA) on asthmatic guinea pigs. Methods: Observe the change of the latent period of asthma inducing of normal guinea pigs and that of them after DYA preventing. Determine the plasma cAMP and cGMP contents. Results: The latent period of asthma inducing was obviously prolonged in guinea pigs prevented by DYA when compared with that of normal guinea pigs and guinea pigs before given DYA ( P
4.Study on identification of cistanche hebra and its adulterants by PCR amplification of specific alleles based on ITS sequences.
Zhen-Hua LI ; Ping LONG ; De-Zhi ZOU ; Yue LI ; Zhan-Hu CUI ; Min-Hui LI
China Journal of Chinese Materia Medica 2014;39(19):3684-3688
To explore the new method of discriminating Cistanche deserticola, Cynomorium songaricum and Orobanche pycnostachya by using PCR amplification of specific alleles. 30 samples of the different C. deserticola, 21 samples of C. songaricum and O. pycnostachya were collected. The total DNA of the samples were extracted, the ITS sequences from C. deserticola, C. songaricum and O. pycnostachya were amplified by PCR and sequenced unidirectionally. These sequences were aligned by using ClustulW. Specific primer was designed according to the ITS sequences of specific alleles, and PCR reaction system was optimized. Additionally, compare with the identification of specific PCR method and DNA sequence analysis method. The result showed that the 331 bp identification band for C. deserticola and the adulterants not amplified bands by a single PCR reaction, which showed good identification ability to the three species. PCR amplification of specific alleles can be used to identify C. deserticola, C. songaricum and O. pycnostachya successfully.
Alleles
;
Cistanche
;
classification
;
genetics
;
DNA Primers
;
genetics
;
DNA, Intergenic
;
genetics
;
DNA, Plant
;
genetics
;
Drug Contamination
;
prevention & control
;
Phylogeny
;
Polymerase Chain Reaction
;
methods
5.Research progress of Mongolian medicine digeda.
Chun-Hong ZHANG ; Chensuyile ; Na ZHANG ; Ping LONG ; Zhen-Hua LI ; Hong ZHU ; Zhen-Wang WANG ; Min-Hui LI
China Journal of Chinese Materia Medica 2013;38(24):4362-4368
Traditional Mongolian medicine Digeda processes a significant importance in clinical therapy with notably actions of heat-clear and detoxication effects. This paper intends to provide comprehensive insight into the species textual research, chemical constituents, qualitative identification, pharmacology and clinical application of Mongolian medicine Digeda to provide valuable data for further studies and the development of clinical applications of these medicinal plants.
Geography
;
Humans
;
Medicine, Mongolian Traditional
;
methods
;
Pharmacology
;
Plants, Medicinal
;
growth & development
6.The etiopathogenisis of fungal infections induced by systemic lupus erythematosus
Zhen TAN ; Xiaomei LI ; Xiangpei LI ; Guosheng WANG ; Long QIAN ; Jinhui TAO ; Yan MA
The Journal of Practical Medicine 2015;(13):2134-2137
Objective To explore the etiopathogenisis of fungal infections induced by systemic lupus erythematosus (SLE). Methods One hundred and forty-seven SLE patients with fungal infections during 2004-2013 were assigned as experimental group and the same number of SLE patients without infections were randomly selected as control group. Clinical and laboratory documents of these patients were comparatively analyzed. Results The fungal infections in SLE patients affected oral mucosa , lower respiratory tract and skin , and the pathogenic bacteria were mainly candida albicans. The wide application of glucocorticoid, immunosuppressants and antibiotics pushed the rise of incidence of fungal infections in SLE patients significantly. Conclusions The patients′ use of glucocorticoid , immunosuppressants and antibiotics attributes to a higher risk of fungal infections , especially infections by candida albicans. Fungus infection may raise the level of C4.
7.Injection of ethanol into the common bile duct to establish a rat model of biliary atresia
Juntao GE ; Long LI ; Yandong WEI ; Haibin WANG ; Guoliang QIAO ; Zhen ZHANG ; Yao LIU ; Anxiao MING
Acta Laboratorium Animalis Scientia Sinica 2014;(3):50-52
Objective To establish a new rat model of biliary atresia by pure ethanol injection into the common bile duct.Methods A catheter was inserted and fixed in the common bile duct in male SD rats .Saline (8 rats) or pure ethanol (16 rats) was injected through the catheter ,respectively, and the biochemical and pathological changes in the rats were examined .Results SD rats in the experimental group were divided into a persistent injury and a restoration of liver dysfunction groups according to pathological and biochemical detection .In the persistent injury group , biochemical impair-ments were significantly higher at 8 weeks after ethanol injection than those in the control group and restoration group .Dis-tinct pathological changes in the liver were observed using HE , SMA, and Masson staining .Conclusions It is a reliable animal model of biliary atresia induced by injection of pure ethanol into the common bile duct in the rat .It will provide a useful tool in future studies of biliary atresia .
8.Combination use of TACE, PVE and HIFU for the treatment of portal vein cancerous thrombus:a clinical study
Yanlei JI ; Zhen HAN ; Limei SHAO ; Yunling LI ; Long ZHAO ; Yuehuan ZHAO
Journal of Interventional Radiology 2015;(3):256-260
Objective To evaluate the combination use of transcatheter arterial chemoembolization (TACE), portal vein embolization (PVE) and high intensity focused ultrasound (HIFU) in treating portal vein tumor thrombus(PVTT). Methods A total of 85 patients with primary hepatocellular carcinoma complicated by PVTT, who were encountered during the period from Jan. 2011 to Feb. 2012 at authors’ hospital, were enrolled in this study. The patients were divided into the study group (n=47) and the control group (n=38). TACE, PVE and HIFU were performed in the patients of the study group, while only TACE and PVE were carried out in the patients of the control group. The therapeutic process was as follows: PVE was carried out 2 weeks after TACE was performed, and for the patients of the study group additional HIFU was conducted about 10 days after PVE procedure. Results The short-term effective rate in the study group and the control group was 89.4% (42/47) and 39.5% (15/38) respectively, and the difference between the two groups was statistically significant (P< 0.05). The 6-month, one-year, and two-year survival rate in the study group were 87.2%(41/47), 66.0%(31/47) and 27.7%(13/47) respectively; the median survival time was 15.4 months. In the control group, the 6-month, one-year, and two-year survival rate were 55.3% (21/38), 39.5% (15/38) and 10.5%(4/38) respectively;the median survival time was 10.3 months. The differences between the two groups were statistically significant (P< 0.05). Conclusion For the treatment of primary hepatocellular carcinoma associated with portal vein tumor thrombus, transcatheter arterial chemoembolization, portal vein embolization together with high intensity focused ultrasound is an safe and effective therapy as it can significantly improve the therapeutic effect and prolong the survival time.
9.Learning curve of laparoscopic-assisted anorectoplasty for high imperforate anus
Jialin LIU ; Xuanzhao WU ; Long LI ; Xu SUN ; Mei DIAO ; Zhen CHEN
Chinese Journal of Applied Clinical Pediatrics 2016;31(12):940-942
Objective To investigate the learning curve of laparoscopy-assisted anorectoplasty in the treatment of high imperforate anus.Methods Sixty patients with high congenital anal atresia who were treated with laparoscopy-assisted anorectoplasty in the Capital Institute of Pediatrics from December 2008 to October 2015 by one experience surgeon were selected as observation group.Based on the criteria,the patients were divided into 4 groups(15 cases in each group) according to the sequence of the operation and every 15 cases served as a learning phase.The operating time,frequency of operation,complication rate,conversion rate to open surgery,blood loss,the rates of blood transfusion and hospital stay were compared among the 4 phases.Results The mean operation duration was (176.3 ±41.6) min,(128.8 ± 36.1) min,(127.3 ± 35.2) min,(124.0 ± 30.1) min in group A,B,C and D,respectively.The operation duration of 4 groups showed a declining trend.Group A has longer operation time than group B,C and D,the difference were statistically significant (P < 0.05).In detail,the comparison of operation time between group A and group B was statistically significant (P < 0.05).Furthermore,group A had significantly more median blood loss than group B,C and D,the differences were statistically significant [the 4 groups of data,respectively,group A:(17.5 ±6.5) mL,group B:(15.0±5.0) mL,group C:(14.5 ±5.5) mL,group D:(8.5 ±6.5) mL,P<0.05].The mean capacity of blood loss was 12.7 mL.No significant difference was found in surgical complications,the rates of blood transfusion,total hospital stay,and postoperative hospital stay among the 4 groups(P > 0.05).But the postoperative hospital stay decreased from 9.8 d (group A) to 7.4 d (group D).Conclusions For a well-trained surgeon in imperforate anus surgery.The learning curve of laparoscopy-assisted anorectoplasty in the treatment of high imperforate anus on about 15 cases helps to make a skillful surgeon.
10.Ethyl acetate-soluble chemical constituents from branch of Hypericum petiolulatum.
Qi ZHAO ; Hai-long ZHANG ; Xue ZHANG ; Xiao-meng LI ; Yu-zhen SUN ; Hang SU
China Journal of Chinese Materia Medica 2015;40(9):1755-1758
The stems and branches of Hypericum petiolulatum were extracted by alcohol and liquid-liquid extraction. Seven furofuran lignans were isolated from the ethyl acetate fraction of ethanol extract of H. petiolulatum by using silica gelchromatography, Sephadex LH-20 chromatography, medium-pressure liquid chromatography and preparative HPLC. Their structures were identified by the spectroscopic methods as pinoresinol (1), medioresinol (2), 8-acetoxypinoresinol (3), epipinoresinol (4), (+)-syringaresinol (5), (+)-1-hydroxysyringaresinol (6) and erythro-buddlenolE (7). All the isolates were firstly found in H. petiolulatum. In the bioassay, compound 7 showed remarkable antioxidative activity inhibiting Fe(+2)-cystine induced rat liver microsomal lipid peroxidation with inhibitory rate 38% at a concentration of 1 x 10(-6) mol · L(-1) (positive control Vit E with the inhibitory rate of 35% at the same concentration).
Animals
;
Antioxidants
;
chemistry
;
isolation & purification
;
pharmacology
;
Drugs, Chinese Herbal
;
chemistry
;
isolation & purification
;
pharmacology
;
Hypericum
;
chemistry
;
Lipid Peroxidation
;
drug effects
;
Microsomes, Liver
;
drug effects
;
metabolism
;
Molecular Structure
;
Oxidative Stress
;
drug effects
;
Plant Stems
;
chemistry
;
Rats