Objective To evaluate the value of clinical findings and different diagnostic criteria for the diagnosis of adult onset Still's disease (AOSD). Methods The AOSD patients admitted to Zhongshan Hospital affiliated to Fudan University from 2003 to 2009 were enrolled. Non-AOSD patients with fever were selected. Different diagnostic criteria of AOSD were applied to all patients. Two indenpendent samples t test and wilco-xon test were used for statistical analysis. Results The clinical features such as rash, arthralgia, arthroncus, sore throat, myalgia, lymph node enlargement, hepatomegaly, splenomegaly, leuko-cytosis, neutrophil ≥80% and serum level of ferrin≥ 2000 ng/ml hadhigher specificity (65.87%~98.41%) with 2.00～5.00 of the positive likelihood ratio.High fever ( ≥39.0℃), negative of ANA and RF had higher sensi-tivity (85.25%~93.65%). Combinations of three parameters such as high fever, rash, sore throat, leukocy-tosis, arthralgia had higher positive likelihood ratio. The specificity of ARA criteria was the highest. The sensitivity and accuracy of Yamaguchi criteria were the highest. Conclusion There is no single parameter that could be specific to the identification of AOSD. Combing with several parameters can improve the diagnostic efficiency. The results of this study have shown that the commonly used diagnostic criteria has high specificity.

Adjuvant and neoadjuvant therapy are the important therapeutic methods for rectal cancer.Neoadjuvant concurrent chemoradiotherapy and postoperative adjuvant chemotherapy play a crutial role in rectal cancer treatment.New chemotherapy drugs and targeted therapy drugs could further improve the therapeutic effect of rectal cancer.

OBJECTIVE:To study the preventive and therapeutic effects of Xinmaitong tablets on model rats with atherosclero-sis (AS) and corresponding mechanism. METHODS:48 rats were randomized into normal group,model group,positive group (simvastatin tablets,2 mg/kg)and the groups of high,medium and low-dose(500,250,125 mg/kg)Xingmaitong tablets,with 8 rats in each group. The rats in all groups except for the normal group were fed high fat diets and given vitamin D3 ip for the estab-lishment of AS model. Meanwhile,the rats in the drug administration groups were given corresponding drugs ig,while those in the normal group and the model group were given normal saline ig,once a day,for 10 consecutive weeks. The levels of cholesterol (TC),triacylglycerol(TG),nitric oxide(NO)and endothelin 1(ET-1)in serum were determined. The pathological change of the aorta tissue was observed under the light microscope,and the size of the aortic atherosclerotic plaque and intima thickness were measured. RESULTS:Compared to the normal group,the rats in the model group had significantly higher levels of TC,TG and ET-1 in serum,a markedly larger aortic atherosclerotic plaque,remarkably thicker intima and much lower level of NO(P<0.01);and obvious aortic atherosclerotic plaque formation was found. Compared to the model group,the above-mentioned indexes of the rats in all drug groups all improved obviously (P<0.05 or P<0.01),except that the decrease in TC in serum of the rats in the group of low-dose Xinmaitong tablets was not significant,which were positively correlated with dose;and the aortic lesion in drug groups was found to be improved. CONCLUSIONS:Xinmaitong tablets have certain preventive and therapeutic effect on the model rats with AS by a mechanism which may be related to the reduction in lipid deposition and the size of an atherosclerotic plaque,the improvement in NO/ET balance and the alleviation of an endothelial injury.

Calcium Compounds ; Foreign Bodies ; complications ; etiology ; Humans ; Infant ; Male ; Oxides ; Respiratory Insufficiency ; etiology ; therapy ; Treatment Outcome

Child, Preschool ; Coffin-Lowry Syndrome ; diagnosis ; psychology ; Diagnosis, Differential ; Humans ; Male

Collateral disease theory has been applied to investigate the pathogenesis of chronic dermal ulcer in traditional Chinese medicine. It is suggested that deficiency of vital qi is the pathological basis of chronic dermal ulcer with collaterals stagnation as the major pathological factor, and collaterals impairment by toxin evil is the main pathological change. The important principle in treatment of chronic dermal ulcer is established as strengthening the body resistance, dredging collaterals and removing toxins, and this enriched the theory of wound healing in traditional Chinese medicine, and has practical value.

Objective To explore the expression and significance of galectin-3 in COPD and lung cancer.Methods Sixty female SD rats were randomly divided into three groups:COPD group,lung cancer group and the control group with 20 rats in each group.COPD group was given tracheal injection of lipopolysaccharide and exposed to daily cigarette to establish the model.Lung cancer group was injected with 3,4-benzopyrene-corn mixture.The control group was fed normally.After 20 weeks,plasma galectin-3 concentration was assessed in each group using ELISA method.Results The galectin-3 average concentrations of COPD group,lung cancer group and the control group were (8.63 ± 0.46) ng/mL,(9.15 ± 0.69) ng/mL and (4.47 ± 0.51) ng/mL.The average concentrations in COPD and lung cancer groups were higher than that of the control group with a statistically significant difference (P＜0.05).Conclusion As expressed in COPD and lung cancer,galectin-3 may own a great significance to the occurrence,development and prognosis of COPD and lung cancer.

Objective:To compare the effect of high-intensity interval training (HIIT) and moderate-intensity continuous training (MICT) on the abdominal visceral fat of obese women.Methods:Sixty-eight obese female college students were randomly divided into an HIIT group ( n=23), an MICT group ( n=22) and a control group ( n=23). The control group was not given any training intervention. The MICT group performed continuous exercise at an intensity of 60% of each person′s maximum oxygen uptake (VO 2max) until 300kJ of work had been performed. Those in the HIIT group performed repeated 4-minute bouts of cycling at 90% of their VO 2max with 3-minute intervals until 300kJ of work had been performed. The interventions lasted 12 weeks. The subjects′ visceral fat (AVFA) and abdominal subcutaneous fat (ASFA) were measured using computed tomography. Whole-body fat mass (FM) and FM in the android, gynoid and trunk regions were detected using dual energy X-ray absorptiometry before and 48 hours after the final session. Results:After the intervention, the average AVFA, ASFA, percentage of fat mass (FM%), whole-body FM, and FM in the abdominal, gluteo-femoral and trunk regions of the HIIT group and MICT group were significantly lower than before the intervention, but there was no significant difference between them. No significant differences were observed in any of the control group′s indexes.Conclusions:Both HIIT and MICT can reduce the abdominal visceral fat of obese female college students, and the effects of the two exercise modes are equivalent.

Objective:To prepare and purify siRNA targeting a proliferation-inducing ligand targeted(APRIL-siRNA),so as to provxde a basis for studying the role of APRIL in human pancreatic cancer.Methods:pET-22b-APRIL was constructed to express APRIL dsRNA of human pancreatic cancer cell line CFPAC-1 in E.coli and the product was purified by chromatography using CF-11 column.APRIL dsRNA was digested by RNaseⅢto prepare APRIL siRNA,then the reaction mixture was loaded onto a DEAE ion exchange chromatography to remove RNaseⅢfrom oligonucleotides,and size exclusion chromatography was used to purify 21 bp siRNA.The purified APRIL siRNA was used to transfect Chinese hamster ovary(CHO)cells and the expression of APRIL in CHO cells was observed under fluorescence microscope Results:APRIL dsRNA was successfully expressed in E.coli after IPTG induction and was purified by CF-11 column.dsRNA was hydrolyzed with RNaseⅢand was purified by DEAE ion exchange chromatography and size exclusion chromatography.15% nondenaturing PAGE and 12% SDS- PAGE confirmed that RNaseⅢwas removed from oligonucleotides and 21 bp siRNA was purified with size exclusion chromatography.It was also found that APRIL siRNA obviously depressed APRIL expression in CHO cells.Conclusion:We have successfully constructed APRIL siRNA targeting APRIL gene of CFPAC-1 cells with in vitro transcription,which provides a basis for knock-down of APRIL gene in CFPAC-1 cells.

Objective To prepare cetuximab-β-glucosidase conjugates and to identify its enzymatic activity and an?tibody activity. Methods Cetuximab andβ-glucosidase were crosslinked by Sulfosuccinimidyl-4-(N-maleimidomethyl) cyclohexane-1-carboxylate (Sulfo-SMCC). Cetuximab-β-glucosidase conjugates and its enzymatic and antibody activity were examined by non-reduced SDS-PAGE, colorimetry and indirect immunofluorescence assay. Results We can see clear bands ofβ-glucosidase, cetuximab, cetuximab-β-glucosidase conjugates through electropherogram. Although the en?zymatic activity of cetuximab-β-glucosidase conjugates was lower than that ofβ-glucosidase (U/L:672.97±46.19 vs 869.50± 57.28,t=5.972,P<0.05) shown by colorimetry assay, it still maintain good enzymatic activity. Under fluorescence micro?scope, we can see the conjugates interacted with human bladder cancer EJ cells are in a red fluorescence. Conclusion Ce?tuximab,β-glucosidase were crosslinked successfully by Sulfo-SMCC without altered its enzymatic and antibody activity.