Objective: To study the spatiotemporal expression pattern of histone acetyltransferase steroid receptor coactivator-1 (SRC1) in developing mouse heart, so as to explore the relationship of SRC1 with heart development. Methods: The normal mouse hearts were collected at E7.5-E18 and 1 day and 3 months after birth; 9 specimens were chosen for each time point to observe the spatial expression of SRC1 by immunohistochemistry technique, and 6 specimens of each time point were used to examine the temporal expression of SRC1 protein by Western blotting technique and to plot the time-dose curve. Results Immunohistochemistry showed no expression of SRC1 in the heart primordium at E7.5; only very weak SRC1 expression was found in the cardiac tube at E8. 5-E9.5. Weak SRC1 expression was found in the trabeculae after E10.5 and relatively strong and widespread expression was found in other heart regions. Western blotting results demonstrated that SRC1 protein expression at E10.5 was significantly lower than those at E11.5 and E12.5 (P<0.05), and was not significantly different with those of other time points after ElO. 5 (P>0.05). SRC1 expression reached the peak at E11. 5-E12.5, and there was no significant difference between the two time points (P>0.05). SRC1 expression gradually decreased after Ell. 5-E12.5 till the adulthood, and there were no significant differences in the expression after E13.5 (P>0.05). Conclusion: Widespread distribution of SRCl is present in the developing mouse heart after ES. 5, and the expression is in a dynamical spatiotemporal pattern, suggesting that SRC1 may take part in the overall regulation of the heart development, and it might has a closer relationship with the early induction of the heart septa.

Objective To study the influence of dietary test designed by Nutritional Department of Henan Provincial People's Hospital on diabetic inpatients' dietary cognition. Methods A total of 134 diabetic inpatients were selected and tested with standard diabetic meaL Self-reporting of oil, salt, staple food, vegetables and meat intake was evaluated. Nonparametric tests were used for statistical analysis. Results There were significant differences in self-reporting of oil,salt,staple food and meat intake between pre and post test (Z= -4.642, -8.700, -2.218, -5. 528,all P<0.05) ; however,no significant change of self reporting vegetable intake was found ( Z = - 1. 385, P > 0. 05 ). Conclusion Dietary test can significantly change patients' cognition about their daily dietary intake.

Objective To draw up the Chinese Words Reading Test and try to use it as way of premorbid intelligence. Methods 373 health middle-elderly people as study subjects, from five communities in Shanghai city, are between age 55 and 85, and their edueational background is junior high school or above, and their MMSE scores are more than 24 points. 34 of the cases have finished CWRT and Weehsler Adult Intelligence Scale (WAIS) Chinese Revised edition. The main analysis indicators of CWRT: CWRT-Ⅰ for number of right reading 50 characters and CWRT-Ⅱ for 50 words. Results Both CWRT-Ⅰ and CWRT-Ⅱ are relative to education level( r = 0.462,0.489, and P < 0.01), while the correlation with age is not signifieant. Performance of the male participants are better than that of the female. The coefficient of correlation between scores of CWRT-Ⅰ and full IQ of WAIS is 0.79. The mean and standard deviation of scores of CWRT-Ⅰ and CWRT-Ⅱ for the normal elder people with different education levels and gender are provided. Conclusion CWRT is not very diffieuh or easy, and is highly relative to score of WAIS. So it can be used as an evaluating tool for premorbid intelligence.

Objective:To compare the cognitive functions between elderly of Shanghai, Hong Kong and San Diego.Method:104 elderly in Shanghai was evaluated by the Chinese version of Mattis Dementia Rating Scale (DRS)and mini-mental state examination (MMSE), the results were compared with that of 104 persons in Hong Kong and 150 elderly participants in San Diago.Result:Compared with Hong Kong participants, Shanghai participants performed significantly higher on one item, but there were significantly differences in five items between Shanghai participants and the San Diego participants which age and education were matched.Conclusion:Some DRS subscales or individual items may be susceptible to cultural differences.

Adult ; Female ; Health Knowledge, Attitudes, Practice ; Health Services Needs and Demand ; Humans ; Male ; Middle Aged ; Occupational Health ; Steel

Objective To evaluate the soft tissue profile in adults with unoperated isolated cleft palate. Methods Twenty patients older than 16 years(median age,18.4) with unoperated isolated cleft palate were enrolled.Soft tissue lateral cephalograms were traced and compared with those of cleft operated patients and class-Ⅰocclusion individuals.The results of various measurements were analyzed with ANOVA by SPSS11.5. Results In adults with unoperated isolated cleft palate,there was restriction at the top of the upper lip,and the antelabrum of upper lip and the lower lip were found normal. Conclusion The soft tissue profile in adults with unoperated isolated cleft palate exhibits almost the same characteristics as the corresponding hard tissue,and there is covering over effect of the soft tissue to some extent.

To summarize the present situation of research on amylolytic lactic acid bacteria and its amylase,and the fermentation technology of amylolytic lactic acid bacteria.The amylolytic lactic acid bacteria have the ability of secreting amylase,can direct ferment starchy material into lactic acid without hydrolysising processes,simplify the production process,and saving the equipment investment,thereby,decrease the cost of production.Amylolytic lactic acid bacteria isolated from traditional fermented food,or from organic waste and kitchen waste.The mechanism of direct utilize starch raw materials by the amylolytic lactic acid bacteria were introduced,the fermentation technology of amylolytic lactic acid bacteria were compared.Meanwhile it was pointed out that through mutation breeding and genetic engineering methods gain the potent amylolytic lactic acid bacteria,and use the advanced fermentation and separation technology can improve the productivity of lactic acid.

ObjectiveTo study possible differences in cognitive dysfunction between left hemisphere injury(LHI) patients and right hemisphere injury(RHI) ones.MethodsCognitive function of 55 LHI patients and 43 RHI patients were examined by aphasia screening test and the Loewenstein Occupational Therapy Cognitive Assessment Battery(LOTCA).ResultsThe scores of RHI group were significant higher than that of LHI group in orientation in place,overlapping figures,spacial perception,praxis(P<0.001-0.005),but significant lower than that of LHI group in visual identification of shapes and visuomotor organization(P<0.001-0.02).The possibilities of RHI group were significant more than that of LHI group in disorientation in time,shape agnosia,disorder in figure-ground identification,unilateral neglect,disordered attention(P<0.005-0.025),but significant fewer than that of LHI group in aphasia,ideomotor apraxia(P<0.005-0.01).The possibilities were no significant differences in disorientation in place,objects agnosia,colour agnosia,spacial agnosia,motor apraxia,constructional apraxia,thinking disorder between RHI group and LHI group(P>0.05).Conclusions There is characteristic cognitive dysfunction after right or left hemisphere injury.

Objective: To survey the content of serum total cholesterol, LDL and HDL in pa- tients with gastric cancer and to investigate the correlation between serum total cholesterol, LDL, HDL and the differentiation of gastric cancer. Methods: 143 patients with gastric cancer were selected and assigned into 3 groups according to pathology grouping after operation: 1) the well-differentiated group, consisting of 16 patients with papillary adenocarcinoma, and 43 patients with tubi-form well-differentiated adenocarcinoma; 2) the moderately differentiated group, including 39 patients with tubiform moderately differentiated adenocarcinoma, 3) the poorly differentiated group, including 20 patients with poorly differentiated adenocarcinoma, and 14 patients with mucinous adenocarcinoma, and 11 patients with signetring cell carcinoma. In addition, 30 patients with gastric ulcer and 30 health adults were selected as control. Veinous blood of every member was collected, and then the content of serum total cholesterol was surveyed by enzymic method, and the contents of serum LDL and HDL were measured by precipitation method. Results: The level of serum total cholesterol, LDL and HDL before operation in patients with gastric cancer decreased significantly than those with gastric ulcer and normal people (P0.05). There was statistically significance among different differentiation in patients with gastric cancer. The differences of levels of serum total cholesterol, LDL and HDL were statistically significant in gastric cancer of different differentiated groups. The contents of serum cholesterol, LDL and HDL in 40 patients with gastric cancer after operation were higher than those before operation obviously (P