Objective To prepare quercetin liposomes and establish a method for determination of its entrapment efficiency.Methods The film dispersion-homogenizing method was used to prepare quercetin liposomes.The formulation was optimized on the basis of orthogonal design and its entrapment efficiency was performed by the protamine sedimentation method.Results The optimal conditions were found to be cholesterol-egg phospholipid=1:3,quercetin-vehicle = 1:40,homogenization pressure 103.4 MPa for three times.The average entrapment efficiency of the optimized nano-liposomes was 92.1%.Conclusion The film dispersion-homogenizing method could be used to prepare quercetin liposomes.The protamine sedimentation method is convenient,accurate,and suitable for the determination of the entrapment effi- ciency of quercetin liposomes.

OBJECTIVETo investigate the effect of oridonin on the human acute lymphocytic leukemia cell line Jurkat and its mechanism.

METHODSJurkat cells were cultured in vitro and treated with various concentrations (0, 1.25, 2.5, 5, and 10 μmol/L) of oridonin for different lengths of time (24, 48, and 72 hours). The proliferation of Jurkat cells was analyzed by MTT assay. The changes in nuclear morphology were evaluated by fluorescence microscopy at 12 hours after treatment with various concentrations of oridonin. The expression levels of Brg1, P53, and C-myc were determined by semi-quantitative Western blot in Jurkat cells treated with various concentrations of oridonin for 24 hours or 5 μmol/L oridonin for various lengths of time (0, 2, 6, 12, and 24 hours). The expression levels of P53 and C-myc and proliferation of Jurkat cells were evaluated after Brg1 expression was knocked down by Brg1-specific siRNA.

RESULTSCompared with the control group, the proliferation of oridonin-treated Jurkat cells was significantly inhibited in a concentration- and time-dependent manner (P<0.05). According to the florescence microscopic analysis, oridonin treatment led to nuclear pyknosis in Jurkat cells. Compared with the control group, Jurkat cells treated with 5 μmol/L oridonin had reduced expression of Brg1 and C-myc but elevated expression of P53. Brg1 knock-down led to a significant reduction in proliferation of Jurkat cells (P<0.05), up-regulated expression of P53, and down-regulated expression of C-myc.

CONCLUSIONSOridonin can inhibit the proliferation of Jurkat cells, probably via the Brg1 signaling pathway.

Antineoplastic Agents, Phytogenic ; pharmacology ; Cell Proliferation ; drug effects ; DNA Helicases ; analysis ; physiology ; Diterpenes, Kaurane ; pharmacology ; Dose-Response Relationship, Drug ; Down-Regulation ; Humans ; Jurkat Cells ; Nuclear Proteins ; analysis ; physiology ; Precursor Cell Lymphoblastic Leukemia-Lymphoma ; drug therapy ; Proto-Oncogene Proteins c-myc ; analysis ; Signal Transduction ; physiology ; Transcription Factors ; analysis ; physiology ; Tumor Suppressor Protein p53 ; analysis

OBJECTIVETo evaluate the possibility for removal of resinifying agent, time required for removal and the working length loss by Resosolv or Chloroform.

METHODS40 human teeth (80 root canals) obturated with FR phenolaldehyde agent were divided into four groups, 20 root canals per group. Group A: Resosolv with K file; group B: chloroform with K file; group C: Resosolv with Ultrasonic K file; group D: Chloroform with ultrasonic K file. Calculating the pereentage for removal of resinifying agent, time required for removal and the working length loss.

RESULTSThe effectiveness of Resosolv for removing resinifying agent was better than chloroform. 87.5% of canals could be negotiated by Resosolv; 45% of canals be negotiated by chloroform.

CONCLUSIONResosolv is an effective solvent for canals obturated with resinifying agent.

Chloroform ; chemistry ; Humans ; Retreatment ; Root Canal Filling Materials ; chemistry ; Root Canal Obturation ; methods ; Solvents ; chemistry

Objective To investigate the mechanism of reverse redistribution (RR) on dipyridamole 201Tl myocardial perfusion studies in the patients with coronary artery spasm. Methods Twenty-six patients with coronary artery spasm and presented as RR on dipyridamole 201Tl myocardial perfusion studies were enlisted as RR group, while other 16 patients with no coronary artery stenosis nor RR were enlisted as control group. Dipyridamole test was repeated during coronary angiography. Corrected thrombolysis in myocardial infarction (TIMI) frame count (CTFC) and TIMI myocardial perfusion grade (TMPG) were measured at RR related and non-RR related coronary arteries before and after dipyridamole infusion respectively.All of the data were analyzed by Student's t-test orχ2-test and correlation analysis. Results Coronary artery angiography showed slower blood flow and lower myocardial perfusion in RR related vessels when compared with non-RR related vessels in RR group, but there was no significant difference among the main coronary arteries in control group. The perfusion defects of RR area at rest were positively related to slowerblood velocity at corresponding coronary arteries ( r = 0.79, t = 10.18, P ＜ 0.001 ). In RR related vessels,CTFC were (36 ±6) frames and (26 ±7) frames (t =4.15, P ＜0.01 ), while TMPG were (2.02 ±0.39)grades and (2.92 ± 0.12) grades ( t = 2.25, P ＜ 0.05 ) before and after dipyridamole infusion, respectively.In non-RR related vessels, CTFC were (29 ±7) frames and (25 ±5) frames (t =2.31, P ＜0.05), while TMPG were (2.56 ± 0.31 ) grades and (2.96 ± 0.06) grades ( t = 2.17, P ＜ 0.05 ) before and after dipyridamole infusion, respectively. However, there were no significant changes of CTFC and TMPG before and after dipyridamole infusion in control group ( t = 0.932, 0.867, respectively, both P ＞ 0.05 ). Conclusion RR is related to the decreased blood flow and myocardial perfusion induced by coronary artery spasm at rest,which may be improved by stress test such as intravenous dipyridamole infusion.

2 in some patients with the loss of high and medium sized vWF multimers in plasma.Eight patients with vWD were identified, wherein two were characterized as type 1,4 as type 2A and 2 as type 3 respectively.Conclusion The panel of tests is suitable for diagnosis and classification of vWD.

Objective To investigate the effect of tigecycline on multi-drug resistant Acinetobacter baumannii ( MDR -AB ) adeB gene expre-ssion and its clinical significance.Methods A total of 60 clinical iso-lates of multidrug-resistant Acinetobacter baumannii were selected, from which 15 experimental strains with positive result to ciprofloxacin ( CIP) efflux pump encoded gene were screened.The minimum inhibitory con-centration ( MIC) before and after given tigecycline was determined using agar dilution assay, the gene of the strains with large change of MIC value was sequenced and compared.Results The lowest MIC of tigecy-cline to MDR-AB was 2 mg? mL-1 .The MDR-AB′s MIC of tigecy-cline at concentration of 0 , 0.62 mg? mL-1 and ciprofloxacin at 1.25 , 2.5 mg? mL-1 were significantly different ( P<0.05).After tigecycline treatment, adeB gene segment had 15 bases mutation, 5 amino acids are replaced and 4 mutant amino acid had no substitution.The adeB gene expression levels before and after the treatment of tigecyclin were (2.51 ±0.72) and (0.87 ±0.21), statistically significant (P<0.05). Conclusion Tigecycline inhibits MDR -AB efflux pump adeB gene expression, lowers adeB expression levels. Other agents with its combination for the treatment of MDR-AB might improve the clinical efficacy.

Objective: To investigate the distribution characteristics of trichlormethane in school drinking water in Jiangsu Province, and to evaluate the health risks and influencing factors of students exposed to trichlormethane, so as to provide a scientific basis for the disinfection and safety of school drinking water. Methods: A total of 315 schools (123 primary schools, 142 junior high schools, 20 high schools, and 30 universities) in Jiangsu Province were selected by a stratified sampling method. Water samples in the wet period (from July to September) of 2023 and in the dry period (from January to March) of 2024 in each school were collected, and 630 drinking water samples were collected. According to the Standard Examination Methods for Drinking Water (GB/T 5750-2023), drinking water samples were analyzed for trichlormethane, and the health risks of trichlormethane exposure in drinking water for students were assessed using the health risk assessment method recommended by US Environmental Protection Agency. The Kruskal-Wallis H rank sum test and Mann-Whitney U test were performed to analyze concentrations and health risks of trichlormethane in school drinking water in different groups. Results: The concentration of trichlormethane in school drinking water in Jiangsu Province was 8.9 (4.6, 14.0) μg/L. The carcinogenic risk of trichlormethane in school drinking water was 9.8×10 -6 (5.3×10 -6 , 1.7×10 -5 ), which was an acceptable low risk. The amount of drinking water per unit body weight and the concentration of trichlormethane in tap water samples were important factors affecting the carcinogenic risk in drinking water for students. Comparison of carcinogenic risks exposed to trichlormethane in drinking water were as follows:primary school students in lower grades had the highest risk of carcinogenesis, with a risk of 1.2×10 -5 , the wet period (1.3×10 -5 ) >the dry period (7.6×10 -6 ), river source water (1.0×10 -5 ) >lake source water (6.8×10 -6 ), liquid chlorine disinfection (1.1×10 -5 ) > sodium hypochlorite disinfection (9.3×10 -6 ), conventional treatment (1.4×10 -5 ) > advanced treatment (9.6×10 -6 ), with statistically significant differences ( Z=88.1, 3.7 , -3.2, -2.7, P <0.05). The non carcinogenic risk of trichlormethane in school drinking water was 1.4×10 -2 for less than 1, and the non carcinogenic risk was acceptable. Conclusions The carcinogenic and non carcinogenic risks of trichlormethane in school drinking water are acceptable in Jiangsu Province, and the primary school students in lower grades are key indicators for risk management of trichlormethane in drinking water. According to the characteristics of the source water, appropriate disinfection methods and water treatment processes are selected to reduce the content of trichlormethane and control health risk.

Objective Coronary features of young smokers and non-smokers with coronary heart disease were compared and the effect of tobacco control education was analyzed. Methods A total of 160 young patients (14-35 years old ) diagnosed with coronary heart disease by coronary angiography were included in this study, patients were followed up for 3 months. There were 118 smokers and 42 nonsmokers, smokers were further divided to psychological counseling intervention group (68 cases) and control group (50 cases ), non-smokers were also divided into psychological counseling intervention group (22 cases) and control group ( 20 cases). Results Incidence of single-vessel lesion (50. 84% vs. 66. 67% )was significantly lower, acute coronary syndrome (75.42% vs. 50. 00% ), double-vessel lesions (24. 58%vs. 19. 05% ), three-vessel lesions ( 11.86% vs. 4. 74% ) as well as coronary artery ectasias ( 12.71%vs. 9. 52% ) was significantly higher in smokers than in non-smokers. Gensini scores (61.94 ±40. 35 vs.45.08 ± 28.97 ) was significantly higher in smokers than in non-smokers ( all P ＜ 0. 05 ). At the end 3-months follow up, smoking cessation rate was significantly higher in psychological counseling intervention group than in control group (61.76% vs. 30. 00%, P ＜ 0. 05 ). New smokers was zero in psychological counseling intervention group and 1 in control group among previou non-smokers. Conclusion Smoking is linked with severe coronary artery lesion in young patients with coronary heart disease and psychological counseling intervention could significantly increase the short-term successful smoking cessation rate in these patients.

Objective To access the prevalence of type 2 diabetes mellitus ( T2DM) and its associated risk factors among adults with obesity in Zhejiang province. Methods The enrolled subjects were selected among local residents aged≥18 years with body mass index≥28 kg/m2 from 15 counties by multi stage stratified cluster random sampling from July to November, 2010. Each participant was required to attend complete questionnaire, physical examination, and testing overnight fasting blood specimen. Results A total of 1 351 residents were enrolled, including 613 males and 738 females. The prevalence of T2DM in adult population with obesity was 15. 03%, being 14. 03% in male, and 15. 85% in female;and that in urban area was 16. 64%, and in rural area was 13. 93%. Data from multivariable logistic regression showed that factors such as ageing (OR=1. 473, 95% CI 1. 243-1. 747), a family history of T2DM(OR=8. 945, 95% CI 5. 481-14. 598), staple food intake (OR=1. 185, 95% CI 1. 017-1. 380), triglyceride(≥1. 7 mmol/L, OR=1. 542, 95%CI 1. 066-2. 232) were risk factors of T2DM;while annual income(OR=0. 695, 95%CI 0. 544-0. 888), and milk intake(OR=0. 750, 95%CI 0. 567-0. 993) were shown as protective factors. Conclusion The prevalence of T2DM in adults with obesity was raised, ageing, a family history of T2DM, staple food intake, and dyslipidemia appeared to be major risk factors for T2DM.

This research was aimed to develop the first level blood information centralized database and real time communication network at a province area in China. Multiple technology like local area network database separate operation, real time data concentration and distribution mechanism, allopatric backup, and optical fiber virtual private network (VPN) were used. As a result, the blood information centralized database and management system were successfully constructed, which covers all the Zhejiang province, and the real time exchange of blood data was realised. In conclusion, its implementation promote volunteer blood donation and ensure the blood safety in Zhejiang, especially strengthen the quick response to public health emergency. This project lays the first stone of centralized test and allotment among blood banks in Zhejiang, and can serve as a reference of contemporary blood bank information systems in China.
Blood Transfusion ; instrumentation ; methods ; standards ; China ; Clinical Laboratory Information Systems ; Computer Communication Networks ; Databases, Factual ; standards ; Humans