Objective To explore the treatment effect and mechanism of A-NK cells on the subcutaneous trans?planted tumor of oral tongue squamous cell carcinoma in nude mice through observing the external growth and hyperplasia of A-NK cells and to provide theoretical evidence for squamous cell carcinoma treatment. Methods A-NK cells and NA-NK cells were both derived from healthy human peripheral blood and cultured in vitro. Cell growth was observed under micro?scope. The squamous cell carcinoma model in nude mice was established through subcutaneous implanting of Tca8113 cells. Then they were randomly assigned into three groups who were injected with either saline solution, or A-NK cells or NA-NK cells paraneoplastically. All animals were sacrificed after 33 days when tumor were isolated then weight and change in tumor size were assessed. Finally curve of tumor growth was drawn. Results Under the microscope, the proliferation of A-NK cells peak in 15 days and NA-NK cells peak in 12 days. After 3 weeks, the number of A-NK cells increased by 39.33 times while the number of NA-NK cells increased by 16.33 times. The Volume of tongue squamous cell carcinoma in saline solu?tion group was larger than that in A-NK cells and NA-NK cells groups, and volume in the NA-NK cells group was larger than that in A-NK cells group. The volume of tongue neoplasms in different groups, time, and interaction effects are statisti?cally significant (P<0.01). The tongue neoplasms weight in the saline solution group was greater than that in the A-NK cells and NA-NK cells group, and the weight in NA-NK cells group was greater than that in A-NK cells group, and the difference are statistically significant (P<0.05). Conclusion A-NK cells and NA-NK cells can significantly inhibit the subcutane?ous transplanted tumors in nude mice and the anti tumor effect of A-NK group is stronger than NA-NK.

Objective To compare the effect of the cochlear stem cells transplantation in treating the noise in-duced hearing loss model and toxic hearing loss model.Methods The Experimental guinea pigs were divided into three groups:the group of the noise induced hearing loss model treated with the cochlear stem cells,the blank con-trol group of toxic hearing loss model treated with the cochlear stem cells and noise induced hearing loss model and the blank control group of toxic hearing loss model.The cochlear stem cells suspension and physiological saline were transplanted into the cochlear inner ears of the three groups,respectively.ABR detection and immunofluorescence observation were implemented three times in 7 days,28days and 56 days after the operation,respectively.Results Nestin positive cells and Myosin ⅦA positive cells were detected in both of the two models treated with cochlear stem cells,but the number of Nestin positive cells and Myosin ⅦA positive cells detected in noise induced hearing loss model was higher than that in the toxic hearing loss model.The ABR responses decreased on the noise induced hearing loss model and toxic hearing loss model treated with cochlear stem cells transplantation,but the hearing re-covery of guinea pigs in the noise induced hearing loss model was superior to the toxic hearing loss model at 28 days and 56 days after operation.Conclusion The cochlear stem cells transplantation is more effective in treating noise in-duced hearing loss than toxic hearing loss model.

Objective To discuss the consequences of two different methods of high pressure injection in CT contrast enhancement to display the aortic dissecting aneurysm.Methods 100 patients underwent Lightspeed 16 MS CT with contrast enhancement of Stellant D high pressure injector(Medrad),injecting speed of 4.0 ml/s and 80 ml dosage of contrast medium.All patients were divided into A and B groups with 50 in each.The single high pressure injection was applied to A group without isotonic Na chloride flush.B group underwent the same high pressure injection and followed by isotonic Na chloride flush.The method of evaluation was carried out by double blind observation.Results A group revealed radiologic artifact up to 40 cases with positive rate of 80%.B group demonstrated the same kind of radiologic artifact in 26 cases with positive rate of 52%.Conclusions Using normal saline(sodium chloride solution)flush after high pressure injection of contrast medium during MSCT angiography is obviously better to demonstrate the internal structures of treatvessels.

Objective To study the immobilization effect and keep the accurate treating position of Body-Fix (R) device in the patients with vertebral metastatic tumor treated by hypofractionated intensitymodulated radiotherapy.Methods From October 2008 to February 2010,six nasopharyngeal carcinoma patients with 10 treated lesion with vertebral metastasis who were treated by hypofractionated intensitymodulated radiotherapy and immobilized by the Body-Fix (R) device were enrolled in this study.Three sets cone beam CT images were taken and recorded when patient was underway the initial setup,position correction and after radiation delivery.Comparing these images with the planning CT images to get the setup errors and the intrafractional position shifting,and the immobilization effect of Body-Fix (R) device was analyzed.Results In the upper,middle and lower sections of the vertebrae,the intrafractional setup errors in the left-right direction were (-0.6±0.5) mm,(-0.1 ±1.0) mm,(0.0±0.4) mm,with in the superior-inferior direction (1.0 ± 1.4) mm,(4.8 ± 5.7) mm,(0.0 ± 0.3) mm and in the anterior-posterior direction (1.2 ± 5.2) mm,(-0.3 ± 0.3) mm,(0.0 ± 0.5) mm,respectively.Conclusions With Body-Fix (R) device,the intrafractional setup errors can be minimized within 2 mm which make the accurate spinal radiosurgery technique possible.

Objective To study the relationship between occupational stress and metal health of radiation workers.Methods Totally 220 radiation workers were investigated by Chinese perceived stress scales questionnaire,Chinese health questionnaire-12,as well as questionnaire related to radiology.Results The stress score of radiation worker with senior title was 18.18 ± 6.12,which was the lowest of all 220 participants.There was positive relationship between stress score and health score (r =0.484).19.1％ of the workers sometimes felt stressed due to the danger of work.Conclusions There are some significantly correlation between occupational stress and metal disorder.More attention should be paid on the occupational stress.Positive measures such as decompression and psychological intervention will be helpful to protect physical and mental health of radiation workers.

Humans ; Male ; Middle Aged ; Tracheal Stenosis

OBJECTIVE To investigate the feasibility of determining the maximum comfortabl level by using the acoustically evoked stapedius reflex threshold via cochlear implant. METHODS Eleve cases of Nucleus C124 cochlear implant recipients operated in our hospital were selected(8-39 years old, who can decide TIC value by themselves with the postoperative time ranging from device initiation to 1 year after operation),of those four cases were diagnosed as bilateral large vestibular aqueduct syndrome,1 case as common cavity dysplasia,.neural response telemetry of these five patients with inner ear dysplasia were very high or barely measurable.Real-MCL level and acoustically evoked stapedius reflex threshold under activated condition were measured respectively. The stimulating sound was 110dB of white noise.RESULTS The two group values of ASRT and real-MCL were found to be consistant .There is no significant difference statistically (P＞0.05). The thresholds of ASR increase with the increasing of real-MCL at the beginning of device initiation. CONCLUSION The acoustically evoked stapedius reflex threshold may reflect (ASRT) the maximum comfortable stimulation level under activated conditions in patients with cochlear implants.it is simple, reliable and may be used to determine the MCL of child cochlear implanted patients.

Animals ; Colorectal Neoplasms ; metabolism ; pathology ; Epithelial-Mesenchymal Transition ; Humans ; Insulin-Like Growth Factor I ; metabolism ; Neoplasm Metastasis ; Signal Transduction ; Transforming Growth Factor beta ; metabolism ; Wnt Signaling Pathway

Objecfive To investigate the expression of erbB4 and PTEN in cutaneous squarnous cell carcinoma(SCC)tissue and their significance.Methods Immunohistochemical Elivision method was used to measure the protein expression of erbB4 and PTEN in tissue specimens from 52 patients with SCC (11 cases with and 41 cases without lymph node metastasis)and 10 normal human controls.Results The positivity rate of erbB4 was significantly higher in the patients with SCC than in the normal human controls (75%vs.10%,x2=12.77,P<0.01),and in the patients with lymph node metastasis than in those without (100%vs.68.29%,P<0.05).Significant differences were also observed in the expression rate of PTEN between the patients with SCC and the normal controls (48.08%vs.100%,x2=9.20,P<0.01),between the patients with well-differentiated SCC and those with poody differentiated SCC(78.57%vs.36.84%,P<0.05),and between the patients with lymph node metastasis and those without (9.09%vs.58.54%,P<0.01).The expression of erbB4 was negatively correlated with that of PTEN (r=-0.42.P<0.01).Conclusion The disturbalice in mutual regulation between erbB4 and FIEN seems to be involved in the initiation.progression and metastasis of SCC.

Objective The objective was to discussing the difference between the Pemberton osteotomy and Salter osteot?omy which performed in patients between the ages of 2 and 3 years who suffered from developmental dislocation of the hip (DDH). Methods A retrospective review of the results of operation treatment for DDH in 59 children (84 hips) from January 1998 to De?cember 2008 was conducted. There are 10 boys (14 hips) and 49 girls (70 hips). The age of the patients was between 2-3 years old at the time of treatment 2.5±0.4 years. Surgery consist of open reduction of the hip, capsulorraphy, shortening and derotational oste?otomy of proximal femur, and innominate osteotomy which include Pemberton osteotomy (33 hips), Salter osteotomy (51 hips). McKay and Severin modified criteria were used to assess the function and radiographic results of the hip. Results The average follow?up time was 5.6±3.5 years ranging from 2 to 16 years. According to Severin criteria at final follow?up, 78 hips (93%) had ex?cellent and good results;4 hips were fair and 2 hips poor result. The radiology results in Salter osteotomy were better than Pember?ton osteotomy (rate of excellent and good results 100%vs. 82%,χ2=7.43, P=0.003). According to the McKay criteria Salter osteoto?my and Pemberton osteotomy have no significant difference in latest follow?up (the satisfactory rate 100%vs. 97%,χ2=1.56, P=0.39). 18 hips (21%) had proximal femoral growth disturbance which 10 hips in Pemberton group, 8 hips in Salter group. There is no significant difference (χ2=2.54, P=0.17). Conclusion Open reduction, innominate osteotomy and proximal femoral osteotomy were effective procedures for the treatment of DDH in children between 2-3 years old. More attention should be taken in Pember?ton osteotomy to prevent the acetabular bony edge absorption.