BACKGROUND:Large randomized controled clinical trials have shown that drug-eluting stents has a lower
restenosis rate compared with bare-metal stents, but the drug-eluting stents cannot reduce major cardiovascular events and death rates, and long-term folow-up of clinical registries show the drug-eluting stents even may
increase late stent thrombosis events.
OBJECTIVE:To evaluate the incidence of stenosis and adverse reactions after treatment with biodegradable, non-biodegradable drug-eluting stents and bare-metal stents in patients after myocardial infarction.
METHODS:We retrospectively analyzed the clinical data of 236 cases of acute myocardial infarction, in which 79 cases were treated with biodegradable sirolimus-eluting stents, 83 cases treated with non-biodegradable
sirolimus-eluting stents, and 74 cases treated with bare metal stents. Then, we compared late lumen loss and restenosis within 12 months after stent implantation and major adverse cardiac reactions within 24 months after stent implantation.
RESULTS AND CONCLUSION: After 12 months, the late lumen loss of the bare stent group was significantly higher than that of biodegradable and non-biodegradable sirolimus-eluting stent groups (P < 0.05), but the
difference in stent restenosis rate (P > 0.05). After 24 months, there were no differences in death, cardiac death, recurrent myocardial infarction, target vessel revascularization, target lesion revascularization, major adverse
cardiac events and stent thrombosis events among the three groups. These three kinds of stents need to be
further studied in their long-term efficacy and safety as percutaneous coronary intervention for acute ST-segment elevation myocardial infarction.

Humans ; Maintenance Chemotherapy ; Multiple Myeloma ; therapy

Objective To observe the effects of neuromuscular electrical stimulation (NMES) at different frequency on post-stroke pharyngeal dysphagia.Methods Forty-five stroke survivors with pharyngeal dysphagia were randomly divided into treatment group 1,treatment group 2 and treatment group 3,eachof 15 patients.Patients in the treatment group 1,2 and 3 received NMES once,twice and three times a day respectively.All the 3 groups were given conventional swallowing training.The swallowing function classification was conducted before treatment,as well as 1,2 and 4 weeks after the treatment.Before and 2 weeks after the treatment,X-ray examination of swallowing (VFSS) was taken to assess the swallowing function,and the average treating days of the 3 groups were compared.Results The swallowing function classification showed that in treatment group 1 and 2,significant improvement was observed at this time point than that at the previous time point (P＜0.05).In treatment group 3,after 1 and 2 weeks of treatment,significant improvement was found compared to those previously,but there was no difference between after 2 weeks and 4 weeks of treatment.At the same time points,the treatment group 2 and 3 showed higher efficiency than the group 1 (P＜0.05).And compared with the treatment group 2,the treatment group 3 showed higher efficiency after 1 and 2 weeks of treatment (P＜0.05),but there was nosignificant difference after 4 weeks of treatment.Two weeks after the treatment,the average VFSS scores of all groups were significantly higher than those before the treatment (P＜0.05) and that of treatmentgroup 2 (7.600± 1.793) and 3 (8.900± 1.636) was significantly higher than that in treatment group 1 (6.700±1.873),as was that of group 3 compared to group 2.Compared with the treatment group 1 and 2,the average treatment days decreased significantly in treatment group 3 (P＜0.05).Conclusion NMES is effective in treating pharyngeal dysphagia after stroke.And within a certain range (1 to 3 times every day),with the increasing of daily treatment frequency,the effectismore significant and the average time of treatment shortens.

Objective To investigate the effects of KangAi injection on apoptosis and proliferation of rectal cancer Lovo cells in vitro.Methods SubG1 and Annexin -V /PI were used to examine the apoptosis of Lovo cells in vitro.Real -time PCR was used to examine the expression of Caspase -3 and Bcl -2 at mRNA level.Proliferation protein ki -67 was detected by flow cytometry.Results Lovo cells treated with KangAi injection 180μL/mL for 48h increased apoptosis rate from (4.75 ±1.04)% to (45.61 ±5.22)% with SubG1 (t =7.68,P <0.01 ),and (7.28 ±1.99)% to (57.02 ±3.88)% with Annexin -V /PI(t =11.42,P <0.01).Lovo cells treated with KangAi injection 180μL/mL for 48h increased the expression of Caspase -3 at mRNA level(t =4.06,P <0.05),while Bcl-2 decreased(t =5.69,P <0.01 ).Lovo cells treated with KangAi injection 180μL/mL for 48h decreased the expression of ki -67 from (95.79 ±1.66)% to (65.84 ±4.80)%(t =5.90,P <0.01 ).Conclusion KangAi injection can promote apoptosis and inhibit proliferation of rectal cancer Lovo cells in vitro.

Objective To explore inhibitory effects of ginsenoside on the proliferation of human thyroid canc-er SW579 cells in vitro and expression of C -myc and Bcl -2 protein.Methods Human thyroid cancer SW579 cells were cultured according to conventional method.The study was divided into the control group and ginsenoside group (20,40,8ug/mL).Inhibitory role of ginsenoside on proliferation of SW579 cells was detected by MTT assay.Western-blot method was used to determine expression levels of C -myc and Bcl -2 protein in different group.Results The inhibition rates of 20,40,80μg/mL ginsenoside to SW579 thyroid carcinoma cell were 22.35%,51.76% and 68.24% respectively.Which meaned ginsenoside had obvious inhibitory effect compared with the control group(P <0.01),and inhibition increased with the increase of solubility(P <0.01).C -myc and Bcl -2 protein were reduced progressively in place with the increase of ginsenoside concentration by Western -Blot analysis (P <0.01 ).Conclusion Gisenoside may play the inhibitory role on proliferation of human thyroid cancer SW579 cells in vitro, and its mechanism may be related with down -regulation of C -myc and Bcl -2 protein.

Objective To observe inhibitory effects of Ginsenoside on the proliferation cultured stem cells of human rectal carcinoma in vitro.Methods Tumor stem cells of rectal carcinoma in human were cultured in vitro and divided into saline water group,reltitrexed group(3mg/mL),and Ginsenoside(25μg/mL)plus reltitrexed(3mg/mL) group,with dosage of qd and for 2 weeks.Cells growth was observed under the microscope,and immunofluorescence staining was used to detect CD +133 expression of rectal carcinoma cells.MTT colorimetric method was taken to measure inhibitory of Ginsenoside on proliferation of tumor stem cells of rectal carcinoma.Apoptosis of tumor stem cells of rec-tal carcinoma lead by Ginsenoside was observed by DAPI staining.Results Part of cells were growing up like ball and CD +133 immunofluorescence staining was positive.Inhibitory role of Ginsenoside plus reltitrexed on CD +133 cells of rectal carcinoma appeared on the first day,which was bigger than that of retitrexed by MTT method (P =0.03).Com-pared with the retitrexed group,Gisenoside combined with reltitrexed reduced CD +133 cells expression of rectal carcino-ma,in which optical density and area density of CD +133 cells were obviously decreased with statistical significance (P =0.007,P =0.006,respectively).Conclusion Gisenoside plays the remarkably inhibitory role on proliferation of CD +133 cells of rectal carcinoma.

BACKGROUND:Chronic ankle instability affects normal life severely. Therefore, chronic ankle instability causes more and more attention of experts, and choice of reasonable and effective diagnosis and targeted treatment method for chronic ankle instability in early stage has become an important issue in the clinic.
OBJECTIVE:To review literature research on the diagnosis and treatment of chronic ankle instability in recent years, and to provide reference and evaluation criteria for the diagnosis and reasonable and effective treatment of chronic ankle instability in clinical practice.
METHODS:We searched PubMed database, China National Knowledge Infrastructure, Wanfang database and VIP database for clinical and basic experimental research on chronic ankle instability. Keywords were“chronic ankle, instability, diagnosis, rehabilitation exercises, surgery methods, research progress”in Chinese and“chronic ankle, instability, diagnosis, rehabilitation exercises, surgical methods, research”in English. Total y 40 literatures were analyzed.
RESULTS AND CONCLUSION:Because of the special anatomical features and biomechanical advantages of the ankle, we should first consider the early diagnosis and effective treatment in chronic ankle instability patients. The treatment should focus on the anatomic characteristics, biomechanic characteristics, etiology of chronic ankle and diagnostic methods of the ankle joint. Conservative treatment and rehabilitation exercises after surgery play a major role in the recovery of chronic ankle instability. The use of balance board, tilting board, and climbing machines is an effective rehabilitation exercises for chronic ankle instability. The application of AOFOS-hind foot and ankle fibula reaction time is important for the assessment of functional recovery of the ankle joint. The emergence of arthroscope is an important clinical research direction of ankle surgery. This plays a significant effect on the treatment of chronic ankle instability.

Objective To explore the changes of the survival quality in stomach cancer patients before and after surgery and its influencing factors.Methods The patoents with stomach cancer have been diagnosed in 202 cases,through the QLQ-C30 scale before another opponent live quality investigation,as well as many variables (conditional Logistic regression) method,the life quality of correlation factors of gastric cancer patients were anelyzed.Results Compared with before treatment,after a month QLQ-C30 various quality of life index compared to baseline state basic recovery,the difference was not statistically significant ( P ＞ 0.05 ),and a week after QLQ-C30 various quality of life index and before treatment is statistically significant ( all P ＜ 0.05 ).Conclusion Gastric cancer patients before and after surgery survival influence factors on the quality of the main factors for the population sociology characteristics,social support,male,operation methods and medical expenses,should strengthen the psychological and social function of patients to improve.

This review introduces the basal elements and structures of Ussing chamber and it′s applications in the field of intestinal barrier function research.

The purpose of the present study was to assess the correlation that likely exists among increased portal pressure (Pp), portal blood flow quantity (Qp) and ETA and ETB receptor mRNA expression in human cirrhosis. In situ hybridization and reverse-transcription polymerase chain reactions (RT-PCR) were performed to determined the expression of ETA and ETB receptor mRNA in liver tissues from traumatic subjects (n = 10) and cirrhotic patients (n = 15) in whom hepatic hemodynamic values were measured. The expression of the two transcripts was significantly higher in liver samples of cirrhotic patients than in those obtained from traumatic subjects. It has shown that ETA receptor mRNA predominantly located in hepatic stellate cells (HSCs) and vascular smooth muscle cells of intrahepatic arteries and portal veins, ETB receptor mRNA in HSCs, sinusoidal endothelial cells and Kuppfer cells. There was a highly significant direct relationship between ETA and ETB receptor mRNA and Pp and Qp in cirrhotic patients. It suggests that liver paracrine endothelin system may be overactivated in human cirrhosis accompanied with increased expression of ETA and ETB receptor mRNA which may play an important role in the pathogenesis and maintenance of splanchnic hyperdynamics.
Gene Expression ; Hemodynamic Processes ; Hypertension, Portal/metabolism ; Liver Cirrhosis/genetics ; Liver Cirrhosis/*metabolism ; Portal Vein/*physiopathology ; Receptors, Endothelin/genetics ; Receptors, Endothelin/*metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; *Splanchnic Circulation/physiology