1.Influence of Pulmonary Hypertension Induced by Left-to-Right Shunt on Pulmonary Collagen Remodeling
zhen-hui, HAN ; xi, ZHANG ; zhen-yu, XIONG ; yong, GAI
Journal of Applied Clinical Pediatrics 2004;0(12):-
Objective To establish a rat model of pulmonary hypertension induced by left-to-right shunt and explore the influence of high pulmonary blood flow on pulmonary vascular collagen remodeling.Methods Abdominal aorta and inferior vena cava shunting was produced in rats. Pulmonary artery meanpressure (PAMP) of each rat was measured by using a right cardiac catheterization.Pulmonary artery collagen Ⅰ and Ⅲ were detected using immunohistochemisty.Results After 11 weeks of shunting the Qp/Qs was 3.3∶1.0,indicating a large shunt. Pulmonary artery mean pressure was increased as compared with controls[(23.0?0.9) mm Hg vs (15.7? 1.1) mm Hg,P
3.Changes of NO and NOS in blood and SaO2 of acclimatized youngs pre- and post-oxygen-enrichment at 3700 m.
Xi-zhou ZHANG ; Jian-hua CUI ; Zhen-de HA
Chinese Journal of Applied Physiology 2004;20(2):149-150
Acclimatization
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physiology
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Adolescent
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Altitude
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Humans
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Male
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Nitric Oxide
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blood
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Nitric Oxide Synthase
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blood
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Oxygen
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physiology
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Pulmonary Gas Exchange
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Young Adult
4.Application of Equipment in Mobile Medical Service Corps and Construction of Maintaining SOP
Yingjun TAN ; Chi ZHEN ; Xi LIU ; Chaoqun ZHANG
Chinese Medical Equipment Journal 2004;0(09):-
The reason for the construction of standard operation process(SOP) in equipment application and maintenance is expatiated.The content and how to construct the system are also discussed.Many problems in equipment application and management are solved.The efficiency of all kinds of equipment in the construction of mobile medical service corps are enhanced.
5.Prognostic values of tumor-stroma ratio and tumor-infiltrating lymphocytes in stagesⅡandⅢcolorectal cancer
Xi ZHANG ; Bailai HU ; Zhen LV ; Guangzhen MA ; Lirong CHEN
Chinese Journal of Clinical Oncology 2015;46(4):241-246
Objective:To investigate the prognostic values of tumor–stroma ratio and tumor-infiltrating lymphocytes in colorec-tal cancer. Methods:A total of 218 stageⅡorⅢprimary colorectal cancer patients were analyzed for the tumor–stroma ratio (TSR) and tumor-infiltrating lymphocytes (TIL) by using HE stained histological sections. The relationship between TSR and clinicopatholog-ic variables and the difference in clinical outcomes of different groups were also analyzed. Results:Overall survival rates for the stro-ma-low group were significantly higher than the stroma-high group in stagesⅡandⅢcolorectal cancer (P<0.05). However, the differ-ences in TSR were not correlated with clinicopathologic features such as gender and age (P>0.05). Overall survival rates of patients with high TIL were significantly higher than patients with low TIL (P<0.05). Patients with high TIL had notable better prognosis than patients with low TIL (P<0.05) in the stroma-low group. Conclusion:TSR and TIL are independent prognostic factors for stagesⅡandⅢcolorectal cancer. The combined estimates of TSR and TIL in routine pathology diagnoses may provide more evidence to predict the prognosis of stagesⅡandⅢcolorectal cancer.
6.Effect of Dexamethasone on bid Gene Expression and Cerebral Apoptosis in Brain after Cerebral Hypoxia-Ischemia
Ai-zhen, WANG ; Zhen-yu, ZHANG ; Yuan-ming, ZHANG ; Xi-rong, GUO ; Shu-ting, LI
Journal of Applied Clinical Pediatrics 2004;0(11):-
Objective To investigate the bid gene expression and cell death in brain after cerebral hypoxia-ischemia in neonatal rats and the effects of dexamethasone(DEX)on bid gene expression,so as to elucidate the possible mechanism of the neuroprotective effect of DEX pretreatment on rats following cerebral hypoxia-ischemia.Methods Twenty-four SD neonatal rats were divided randomly into hypoxia-ischemia brain damage(HIBD),normal,dexamethasone-pretreated and 9 g/L NaCl(NS)control group.The animal models of HIBD were made.Total RNA from ipsilateral cerebral hemisphere was extracted.Reverse transcription polymerase chain reaction(RT-PCR)was used to evaluate the level of bid gene expression after hypoxia-ischemia.Cerebral apoptosis was determined by terminal-deoxynucleotidy transferase mediated d-UTP nick end labeling(TUNEL).Results The levels of bid mRNA were higher in HIBD rats than those in normal rats.The number of positive apoptosis cells significantly increased in HIBD group(P
7.Influence of Dexamethasone on Cellular Inhibitor of Apoptosis Protein 1 Gene Expression and Caspase-3 Activity in Brain after Cerebral Hypoxia-Ischemia
Ai-zhen, WANG ; Zhen-yu, ZHANG ; Yuan-ming, ZHANG ; Xi-rong, GUO ; Shu-ting, LI
Journal of Applied Clinical Pediatrics 2004;0(12):-
Objective To explore the cellular inhibitor of apoptosis protein 1(cIAP1)gene expression and Caspase-3 activity in brain after cerebral hypoxia-ischemia in neonatal rats and the influence of dexamethasone(DEX)on cIAP1 gene expression and Caspase-3 activity,so as to elucidate the possible mechanism of the neuro-protective effect of DEX pretreatment on rats following cerebral hypoxia-ischemia.Methods Twenty-four SD neonatal rats were divided randomly into hypoxic-ischemic brain damage group(HIBD group),normal group(NS group),dexamethasone-pretreated group(DEX group)and 9 g/L NaCl control group(NS group).The animal models of HIBD were made.Total RNA from ipsilateral cerebral hemisphere was extracted.Reverse transcription polymerase chain reaction(RT-PCR)was used to evaluate the level of cIAP1 gene expression after hypoxia-ischemia.Caspase-3 relative activity of brain tissue was determined by colorimetric assay.Results The levels of cIAP1 mRNA were lower in HIBD group than those in NS group.Caspase-3 relative activity significantly increased in HIBD group(P
9.Tongue base surgery with front neck access and uvulopalatopharyngoplasty for treatment of severe obstructive sleep apnea-hypopnea syndrome.
Qing-Quan ZHANG ; Xi-Cheng SONG ; Tian-Zhen ZHANG ; Yan SUN ; Hua ZHANG
Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(9):704-705
Adult
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Aged
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Female
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Humans
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Male
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Middle Aged
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Otorhinolaryngologic Surgical Procedures
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methods
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Sleep Apnea, Obstructive
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surgery
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Tongue
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surgery
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Uvula
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surgery
10.Study on optimization of the preparation for citrus oils β-cyclodextrin microspheres inclusion compound by using the response surface methodology
Yan ZHANG ; Jing SONG ; Zhen WANG ; Xifeng ZHAI ; Han ZHANG ; Xi ZHANG ; Lili WU ; Junru LIU
International Journal of Traditional Chinese Medicine 2021;43(1):48-53
Objective:The response surface methodology (RSM) and Box-Behnken design were adopted to optimize the preparation for citrus oils β-cyclodextrin microspheres inclusion compound. And the physical characterization and heat stability were evaluated.Methods:The best preparation technology included inverse emulsion polymerization and saturated water solution method, with volatile oil weight ratio and microspheres, microspheres and water feeding ratio, inclusion temperature as impact factors, inclusion rate as the response value, establish regression model,. We explored the orange peel naphtha beta ring paste by microscopy, infrared spectroscopy (IR), differential scanning calorimetry (DSC) and heat stability test.Results:The best preparation technology included the essential oil with beta ring paste microspheres ( V: m), the ratio of 1:10, beta ring paste small ball and the ratio of water (m: V) for 1:15, and inclusion temperature for 41 ℃. The average encapsulation efficiency and the average rate of yield under optimized conditions were 62.21% and 85.24%, respectively. The physical characterization and thermal stability tests demonstrated that the β-cyclodextrin microsphere inclusion complex of volatile oil from Citrus was successfully prepared and the physical properties were stable. Conclusion:The preparation method of citrus oils β-cyclodextrin microspheres inclusion compound by using the response surface methodology.