Objective To explore particularity of the diagnosis and treatment with massive bilateral chronic subdural hematoma (BCSDH) in elders.Method The clinical data of 25 elders with BCSDH treated in our hospital from 2007 to 2011 were analyzed retrospectively.All patients were examined with computerized tomography (CT) of 25 patients through the bilateral trephined hole.Results Twenty-four patients recovered better,the symptom significantly improved.After operative 1 month,14 patients? hematoma disappear,6 patents only had little bilateral subdural effusion,4 patents only had little unilateral subdural effusion.One patient appeared massive subdural effusion on the second day after operation,the familiality gave up therapy,and the patient was dead.Conclusion CT scanning should be taken before you make correct diagnisis.Bilateral single twist-drill craniostomy with irrigation and drainage is a chief method to massive BCSDH.

Objective To observe the effect of cupping plus Chinese medicinal fumigation on expectoration in patients with chronic obstructive pulmonary disease (COPD) due to phlegm-heat obstructing the lung. Method Eighty-two patients with COPD due to phlegm-heat obstructing the lung were randomized into a treatment group and a control group, 41 cases in each group. The control group was given regular Western medications, while the treatment group was intervened by cupping plus Chinese medicinal fumigation in addition to the treatment given to the control group, once a day, 5 d as a treatment course. The therapeutic efficacies were observed after 2 treatment courses. Result The clinical effective rate of the treatment group was higher than that of the control group, with a statistical significance (P<0.05). Conclusion Cupping plus Chinese medicinal fumigation can enhance the expectoration in patients with COPD due to phlegm-heat obstructing the lung.

Objective To explore inhibitory effects of ginsenoside on the proliferation of human thyroid canc-er SW579 cells in vitro and expression of C -myc and Bcl -2 protein.Methods Human thyroid cancer SW579 cells were cultured according to conventional method.The study was divided into the control group and ginsenoside group (20,40,8ug/mL).Inhibitory role of ginsenoside on proliferation of SW579 cells was detected by MTT assay.Western-blot method was used to determine expression levels of C -myc and Bcl -2 protein in different group.Results The inhibition rates of 20,40,80μg/mL ginsenoside to SW579 thyroid carcinoma cell were 22.35%,51.76% and 68.24% respectively.Which meaned ginsenoside had obvious inhibitory effect compared with the control group(P <0.01),and inhibition increased with the increase of solubility(P <0.01).C -myc and Bcl -2 protein were reduced progressively in place with the increase of ginsenoside concentration by Western -Blot analysis (P <0.01 ).Conclusion Gisenoside may play the inhibitory role on proliferation of human thyroid cancer SW579 cells in vitro, and its mechanism may be related with down -regulation of C -myc and Bcl -2 protein.

Objective To observe inhibitory effects of Ginsenoside on the proliferation cultured stem cells of human rectal carcinoma in vitro.Methods Tumor stem cells of rectal carcinoma in human were cultured in vitro and divided into saline water group,reltitrexed group(3mg/mL),and Ginsenoside(25μg/mL)plus reltitrexed(3mg/mL) group,with dosage of qd and for 2 weeks.Cells growth was observed under the microscope,and immunofluorescence staining was used to detect CD +133 expression of rectal carcinoma cells.MTT colorimetric method was taken to measure inhibitory of Ginsenoside on proliferation of tumor stem cells of rectal carcinoma.Apoptosis of tumor stem cells of rec-tal carcinoma lead by Ginsenoside was observed by DAPI staining.Results Part of cells were growing up like ball and CD +133 immunofluorescence staining was positive.Inhibitory role of Ginsenoside plus reltitrexed on CD +133 cells of rectal carcinoma appeared on the first day,which was bigger than that of retitrexed by MTT method (P =0.03).Com-pared with the retitrexed group,Gisenoside combined with reltitrexed reduced CD +133 cells expression of rectal carcino-ma,in which optical density and area density of CD +133 cells were obviously decreased with statistical significance (P =0.007,P =0.006,respectively).Conclusion Gisenoside plays the remarkably inhibitory role on proliferation of CD +133 cells of rectal carcinoma.

Objective]To summarize Professor Wang’s clinical thoughts and experience of herb pairs in treating“cough caused by wind”. [Methods]To collect and analyse the theory of Wang’s experience of herb pairs in treating“cough caused by wind”by studying from him for a long time. [Results] Professor considered that“cough caused by wind”is a kind of disease characterized by nature of “wind”, which is mainly cough, with wind syndrome unique performance. It is similar to airway hyperresponsiveness diseases,including modern medicine on the infection cough, cough variant asthma, gastroesophageal reflux cough, upper airway cough syndrome, eosinophilic bronchitis, allergic cough, etc, which should be treated by the way of “dispelling wind ventilating lung and relieving cough ”. According to different wind cough sydromes, choose different herb couples.“Feixingcao-Zicao-Xiancao”,“Jiegeng-Shegan”,“Zhipipaye -Zhiziwan -Zhidonghua”,“Zhimahuang -Suye”,“Xinreng -Zhebei”,“Shengbaishao -Shenggancao”are the most commonly used herb pairs in practice.[Conclusion] Professor’s experience of herb pairs in treating“cough caused by wind”is well worth learning for it always gets outstanding effect.

Humans ; Rectal Fistula ; surgery ; therapy

Aged ; Apraxias ; physiopathology ; Gait Apraxia ; physiopathology ; Humans ; Parkinson Disease ; diagnosis ; physiopathology

OBJECTIVE:To establish a method for content determination of principal agents in kanamycin sulfate eye drops by dye colorimetry.METHODS:On the basis of the spectrometric features of ion pair compound produced in the reaction between kanamycin and bromcresol green in buffer solution with pH value of6.0,absorbance was detected at the wavelength of432nm and content was determined.RESULTS:The detected concentration of Kanamycin showed good linear relationship with its absorbance(r=0.9998)within the range of18.0～72.0?g/ml.The average recovery was99.3%(RSD=1.20%).CONCL_ USION:The established method is rapid,simple and accurate,and it can be used for quick test of this preparation during the production.

Bone marrow stem cells(BMSCs) can differentiate into cardiomyocytes when induced with proper reagent. The cardiomyocytes from BMSCs have cardiomyocyte phenotypes with the expression of cardiac-specific antigen and receptor. BMSCs differentiate into cardiomyocytes in host myocardial microenvironments. Mechanism of the differentiation is unclear and need to be studied further.

According to the concept of sagittal spinal balance, the alternating curves of cervical and lumbar lordosis and thoracic and sacral kyphosis enable the head to be positioned over the trunk and pelvis. But the sagittal imbalance represents departure from this ideal form. Cervical kyphosis may represent the most disabling of these imbalances, for it can cause pain, postural difficulties, or neurologic deficit. A summary and discussion is reviewed regarding the anatomy, biomechanics, etiology, clinical presentation, laboratory examination and treatment of this condition.