microRNAs are a class of highly conserved small non-coding RNAs with single-strand,which regulate their target mRNAs at post-transcriptional level.They have been shown to play an important role in the regulation of development of hemopoietic system malignancies.Evading apoptosis is a hallmark of tumorigenesis.In recent years,accumulative data have proved that aberrant expression of microRNAs participates in the regulation of many crucial pathways of apoptosis.By binding to their target mRNAs,microRNAs may inhibit apoptosis (e.g.miR-17-92 cluster,miR-21) or promote apoptosis (e.g.miR-15a/16-1,miR-150),which act as oncogene or tumor suppressor.In addition,the differential expression of microRNAs in lymphoma may provide new markers in determination of prognostic factors.Moreover,insights into microRNA expression in lymphoma may reveal potentially novel therapeutic targets.

The several questions in Theories of Acupuncture and Moxibustion of Different Schools, "the twelfth five-year plan" teaching material, are analyzed and discussed. The expression of DOU Han-qing on "playing a role of meridians, collaterals, qi and blood" is not so accurate, which is suggested to modify as "attaching the importance to needles". "Theory of heaven, human being and earth" "theory of acupuncture on qi regulation" and "the eight therapeutic methods in treatment of disease" in Jinzhenfu (Ode to Golden Needles) are valuable academically and suggested to be supplemented. The expression of XI Hong on "reinforcing and reducing technique by following or against the running course of meridian" and the expression of XU Feng on "the magic turtle eight techniques" are not concrete. It is required to elaborate them in detail. These questions are put forward and needed to discuss with the editors so as to perfect the teaching material.
Acupuncture ; education ; instrumentation ; Humans ; Moxibustion ; Teaching Materials

Objective To study the influence of HBV infection for gastromucous membrane in chronic hepatitis B and cirrhosis and its pathogenesis.Methods The liver biopsy,gastroscope,liver function and HBVM were detected at the same time in 60 patients with chronic hepatitis B and cirrhosis.HBsAg and HBcAg in the tissues of liver and gastromucous membrane were detected by SP immunohistochemistry.Results The damage of gastromucous membrane were found in 56 of 60 patients.Its damage degree was showed as moderate and severe.The other 34 showed mild and moderate damage(P

OBJECTIVETo explore the expression of the epithelial cell adhesion molecule (EpCAM) in prostate cancer (PCa) and its clinical significance.

METHODSWe collected tissue samples from 63 cases of PCa, 46 cases of prostatic intraepithelial neoplasia (PIN), and 58 cases of benign prostatic hyperplasia (BPH) adjacent to PCa and determined the expression of EpCAM in the epithelial and stromal cells by immunohistochemistry.

RESULTSThe positive expression rates of EpCAM in the epithelial cells were significantly higher in PCa and PIN than in PCa-adjacent BPH (98. 4 and 97. 8 vs 51.7%, P <0. 01), and so was that in the stromal cells of PCa than in those of PCa-adjacent PIN (89.5 vs 50.0%, P <0.01). The expression of EpCAM.was remarkably higher in the stromal cells of bone metastasis than in those of non-bone metastasis tissue (100. 0 vs 40. 0%, P <0. 01) but showed no statistically significant differences between the highly and poorly differentiated PCa tissues (88.5 vs 91.9%, P >0.05).

CONCLUSIONThe expression level of EpCAM in the stromal cells of PCa is related to the occurrence, progression, and bone metastasis of the tumor, and therefore may be used as a marker in the early diagnosis of PCa as well as a predictor of bone metastasis of the tumor.

Antigens, Neoplasm ; metabolism ; Biomarkers ; metabolism ; Bone Neoplasms ; metabolism ; secondary ; Cell Adhesion Molecules ; metabolism ; Disease Progression ; Epithelial Cell Adhesion Molecule ; Epithelial Cells ; metabolism ; Humans ; Immunohistochemistry ; Male ; Prostatic Hyperplasia ; metabolism ; Prostatic Intraepithelial Neoplasia ; metabolism ; Prostatic Neoplasms ; metabolism ; Stromal Cells ; metabolism

Female ; Humans ; Infant ; Meningitis, Bacterial ; complications ; Salmonella Infections ; complications ; Salmonella typhi ; isolation & purification ; Sepsis ; complications

Adolescent ; Adult ; Child ; Chronic Disease ; Female ; Humans ; Male ; Middle Aged ; Mucin-5B ; biosynthesis ; Nasal Mucosa ; metabolism ; Sinusitis ; metabolism ; Young Adult

Objective To construct a cDNA library from Hepialus armoricanus parasitized by Cordyceps sinensi. Methods Total RNA was extracted from Hepialus armoricanus parasitized by Cordyceps sinensi and mRNA was purified, cDNA was synthesized by reverse transcription, and cDNA fragments larger than 1kb were ligated with dephosphorylated arms of pBluescriptⅡ SK(+) XR vectors. The recombinant vectors were eletroporated into DH10B.The size of cDNA inserts and the diversity of library were detected by PCR. Results The cDNA library of stroma contained 1.28?106 recombine clone,that of mycelium was 1.0?106 recombine clone. The rate of recombination of stroma was 87.5%,that of mycelium was 68.75%. The insert size ranged from 0.5 to 2 kb. Conclusion The cDNA library Hepialus armoricanus parasitized by Cordyceps sinensi has been constructed successfully.

0.05).There existed a increased trend of the above parameters in group B.(2) Time of weaning from mechanical ventilation was shortened in group A than that in group B(P0.05),but were significant(P0.05).Conclusion Standardized nursing can increase the therapeutic effect on MG by reducing MG crisis and complication incidence,and improving quality of life of MG patients.

Objective To investigate the effects of artervenous fistula on structure and function of heart in patients with hemodialysis in the Second Clinical Hospital of Chinese Medical University from 2004 to 2005.Methods AVF、LAD、RVD、LVD、LVEDV、SV and EF were examined and analysed before and 8 weeks after AVF in 55 patients with hemodialysis.Results LAD、RVD、LVD、LVEDV after AVF increased significantly and EF reduced evidently after AVF.Conclusion AVF can cause extension of LAD、 RVD、 LVD and decrease of EF.

Objective:To explore the central mechanism of working memory impairments experienced by patients with schizophrenia.Methods:Using block-design,ten normal and twenty patients with schizophrenia perform digital recognition(remember two or five digitals simultaneous and make judgment about whether the digital presented latter is that appeared before)and direction judgment of arrows(toward to left or right)while proceeding blood-oxygen level dependent contrast functional magnetic resonance imaging(BOLD-fMRI).Results:On the condition of same cognitive load,abnormal activation of basal ganglia occurred in schizophrenics.Comparing to normal control,patients with schizophrenia exhibited a tendency of more activation in dorsolateral prefrontal cortex.Conclusion:Abnormal activity of basal ganglia and dorsolateral prefrontal cortex may be the critical cause of working memory deficit experienced by patients with schizophrenia.