microRNAs are a class of highly conserved small non-coding RNAs with single-strand,which regulate their target mRNAs at post-transcriptional level.They have been shown to play an important role in the regulation of development of hemopoietic system malignancies.Evading apoptosis is a hallmark of tumorigenesis.In recent years,accumulative data have proved that aberrant expression of microRNAs participates in the regulation of many crucial pathways of apoptosis.By binding to their target mRNAs,microRNAs may inhibit apoptosis (e.g.miR-17-92 cluster,miR-21) or promote apoptosis (e.g.miR-15a/16-1,miR-150),which act as oncogene or tumor suppressor.In addition,the differential expression of microRNAs in lymphoma may provide new markers in determination of prognostic factors.Moreover,insights into microRNA expression in lymphoma may reveal potentially novel therapeutic targets.

The several questions in Theories of Acupuncture and Moxibustion of Different Schools, "the twelfth five-year plan" teaching material, are analyzed and discussed. The expression of DOU Han-qing on "playing a role of meridians, collaterals, qi and blood" is not so accurate, which is suggested to modify as "attaching the importance to needles". "Theory of heaven, human being and earth" "theory of acupuncture on qi regulation" and "the eight therapeutic methods in treatment of disease" in Jinzhenfu (Ode to Golden Needles) are valuable academically and suggested to be supplemented. The expression of XI Hong on "reinforcing and reducing technique by following or against the running course of meridian" and the expression of XU Feng on "the magic turtle eight techniques" are not concrete. It is required to elaborate them in detail. These questions are put forward and needed to discuss with the editors so as to perfect the teaching material.
Acupuncture ; education ; instrumentation ; Humans ; Moxibustion ; Teaching Materials

Objective To study the influence of HBV infection for gastromucous membrane in chronic hepatitis B and cirrhosis and its pathogenesis.Methods The liver biopsy,gastroscope,liver function and HBVM were detected at the same time in 60 patients with chronic hepatitis B and cirrhosis.HBsAg and HBcAg in the tissues of liver and gastromucous membrane were detected by SP immunohistochemistry.Results The damage of gastromucous membrane were found in 56 of 60 patients.Its damage degree was showed as moderate and severe.The other 34 showed mild and moderate damage(P

Female ; Humans ; Infant ; Meningitis, Bacterial ; complications ; Salmonella Infections ; complications ; Salmonella typhi ; isolation & purification ; Sepsis ; complications

Adolescent ; Adult ; Child ; Chronic Disease ; Female ; Humans ; Male ; Middle Aged ; Mucin-5B ; biosynthesis ; Nasal Mucosa ; metabolism ; Sinusitis ; metabolism ; Young Adult

OBJECTIVETo explore the expression of the epithelial cell adhesion molecule (EpCAM) in prostate cancer (PCa) and its clinical significance.

METHODSWe collected tissue samples from 63 cases of PCa, 46 cases of prostatic intraepithelial neoplasia (PIN), and 58 cases of benign prostatic hyperplasia (BPH) adjacent to PCa and determined the expression of EpCAM in the epithelial and stromal cells by immunohistochemistry.

RESULTSThe positive expression rates of EpCAM in the epithelial cells were significantly higher in PCa and PIN than in PCa-adjacent BPH (98. 4 and 97. 8 vs 51.7%, P <0. 01), and so was that in the stromal cells of PCa than in those of PCa-adjacent PIN (89.5 vs 50.0%, P <0.01). The expression of EpCAM.was remarkably higher in the stromal cells of bone metastasis than in those of non-bone metastasis tissue (100. 0 vs 40. 0%, P <0. 01) but showed no statistically significant differences between the highly and poorly differentiated PCa tissues (88.5 vs 91.9%, P >0.05).

CONCLUSIONThe expression level of EpCAM in the stromal cells of PCa is related to the occurrence, progression, and bone metastasis of the tumor, and therefore may be used as a marker in the early diagnosis of PCa as well as a predictor of bone metastasis of the tumor.

Antigens, Neoplasm ; metabolism ; Biomarkers ; metabolism ; Bone Neoplasms ; metabolism ; secondary ; Cell Adhesion Molecules ; metabolism ; Disease Progression ; Epithelial Cell Adhesion Molecule ; Epithelial Cells ; metabolism ; Humans ; Immunohistochemistry ; Male ; Prostatic Hyperplasia ; metabolism ; Prostatic Intraepithelial Neoplasia ; metabolism ; Prostatic Neoplasms ; metabolism ; Stromal Cells ; metabolism

Objective To explore the current situation of nursing students' leadership,in order to improve the clinical leadership of nursing students and provide reference for nursing teaching.Methods In July 2016,a questionnaire survey of nursing students including undergraduate and junior college students in our hospital was conducted by using the Self Assessment Leadership Instrument.SPSS 17.0 was used to conduct the t test to investigate the nursing leadership differences between the two groups.Results The total score of nursing students was (114.68 ± 18.25),and the score of undergraduate students was higher than junior college students (t=8.13,P=0.000);In the dimensions of critical thinking,decision-making skill,interpersonal relationship,team spirit and working relationship,there were significant differences in the scores of undergraduate and junior college students with different levels of education (P＜0.05).Conclusion The level of leadership of nursing students is low.In the process of nursing students' education,we should carry out the leadership courses in the school and practice leadership in clinical clerkship,so that we can promote the improvement of nurses' leadership and promote the development of nursing science.

Diabetes mellitus has became a growing public health problem,its acute and chronic complications threaten the patient's dramatically.HbA1c is recognized as the gold standard for blood glucose evaluation in patients with diabetes It has reported that the incideut rafe of diabetes related will decline remarkably when HbA1c decline by 1％,so the detection accuracy of the HbA1c is very important in the diabetes patient's management.Uncertainty of measurement can reflect the detation accuracy,which will benifite the value of HbA1c.

Objective To construct a cDNA library from Hepialus armoricanus parasitized by Cordyceps sinensi. Methods Total RNA was extracted from Hepialus armoricanus parasitized by Cordyceps sinensi and mRNA was purified, cDNA was synthesized by reverse transcription, and cDNA fragments larger than 1kb were ligated with dephosphorylated arms of pBluescriptⅡ SK(+) XR vectors. The recombinant vectors were eletroporated into DH10B.The size of cDNA inserts and the diversity of library were detected by PCR. Results The cDNA library of stroma contained 1.28?106 recombine clone,that of mycelium was 1.0?106 recombine clone. The rate of recombination of stroma was 87.5%,that of mycelium was 68.75%. The insert size ranged from 0.5 to 2 kb. Conclusion The cDNA library Hepialus armoricanus parasitized by Cordyceps sinensi has been constructed successfully.

Objective To Observe the effects of tumor necrosis factor ? (TNF ?) on cell proliferation and Intracellular free calcium concentraction ([Ca 2+ ]i) in endothelium of human umbilical vein endothelial cells (HUVEC) and investigate the pathogenesis of pregnancy induced hypertension syndrome (PIH). Methods Confluent monolayer of HUVEC was directly incubated with TNF ? at following final concentrations: 500, 1 000, 2 000 U/ml for 24 hours. The percentages of different cellcycles and [Ca 2+ ]i were measured by flow cytometry and fluorospectrophotometry. Results Incubated with TNF ?, the endothelial cells were elongated and transformed into fibroblast like cells. Border of nucleus was sharp, clarity, and cells were in regular shape. But there were abnormal granules in cytoplasma and some cells detached at the concentrotion of 2 000 U/ml of TNF ?. Stimulated by TNF ?, the percentage of cellcycles from phase G 0+G 1 to S and G 2+M decreased significantly and it was dose dependent. [Ca 2+ ]i increased significantly and dose dependent as well. Conclusion TNF ? may injure endothelium directly and inhibit its proliferation and repair through the changes of [Ca 2+ ]i level. It may play an important role in the pathogenesis of PIH.