OBJECTIVE:To establish the RP-HPLC method for determination of the content of 10-deacetyl-bacratin Ⅲ in Taxus baccata.METHODS:The determination was performed on Shim-Pack VP-ODS column (250 mm?4.6 mm,5 ?m) at a column temperature of 40 ℃.The mobile phase consisted of acetonitrile-water (gradient elution) at a flow rate of 1.0 mL?min-1.The detective wavelength was set at 232 nm. RESULTS:The linear range of 10-deacetyl-bacratin Ⅲ was 264～1 320 ng(r=0.999 5)and the average recovery was 98.8%(RSD=1.3%,n=9).CONCLUSION:The method is simple,accurate and reproducible,and it is applicable for the quality control of T.baccata.

Objective: To extract the syndrome elements of CHD angina pectoris with entropy-based complex system partition method and to analyze the distribution of the syndrome elements, in an effort to study the pathogenetic characteristics of CHD angina pectoris. Methods: The clinical data of 403 patients with CHD angina pectoris were collected by clinical epidemiological survey. Entropy-based Complex System Partition was used to extract the syndrome elements, contribution degree of symptoms to syndrome and diagnostic threshold. The combination rule of the syndrome elements was also analyzed. Results: CHD angina pectoris had the following basic syndromes: Qi Deficiency syndrome, Qi Stagnation syndrome, Blood Stasis syndrome, Phlegm Turbid syndrome, Phlegm-fire syndrome, Stagnation-fire syndrome, Yin Deficiency syndrome and Yang Deficiency syndrome. With the increase of syndrome elements, the frequencies of Qi Deficiency syndrome, Yin Deficiency syndrome, Qi Stagnation syndrome, Blood Stasis syndrome, and Phlegm turbid syndrome also increased. Qi Deficiency syndrome and Blood Stasis syndrome were the two syndrome elements seen most frequently. Conclusions: The syndrome elements extracted by entropy-based complex system partition method and their distribution rule can reflect the pathogenic characteristics of angina pectoris, which lays a base of quantify the diagnostic criteria of the angina pectoris syndrome.

Objective: To investigate the changes of brain metabolites of apparent diffusion coefficient (ADC) in infarct area after acute cerebral infarction and the correlation between the ADC values and brain metabolites. Methods: Thirty-two consecutive patients with acute cerebral infarction were enrolled in the study. They were all performed head MR scan, magnetic resonance diffusion weighted imaging (DWI) , ADC map and magnetic resonance spectroscopy (MRS). The ADC values and the content of N-acetyl aspartate (NAA), choline, creatine, and lactic acid were measured in the central and peripheral area of infarction, peripheral normal brain tissue, and the contralateral mirror image area of the central area of infarction (mirror image area). The correlation analyses between ADC values and NAA, choline, creatine, and lactic acid were performed. Results: Circled digit oneCompared with the contralateral mirror image area, the ADC value decreased significantly in the central area of infarction, and the ADC value decreased mildly in the peripheral area (P < 0.01). The ADC values did not change significantly in the peripheral normal brain tissue. Circled digit twoCompared with the contralateral mirror image area, the NAA content decreased significantly and the lactic acid content increased significantly in the central area of infarction. The NAA content decreased mildly and the lactic acid content increased mildly in the peripheral area. There were no significant changes in the NAA and lactic acid in the peripheral normal brain tissue. There were no significant differences in the choline content in the peripheral area of infarction, peripheral normal area and mirror image area, but they were all higher than that in the central area of infarction, and the differences were significant (P <0.05, P < 0.01). Circled digit threeAfter acute cerebral infarction, the ADC value of brain tissue was positively correlated with the NAA content (rs = 0.196, P = 0.027), negatively correlated with the lactic acid content (rs = - 0.381 , P = 0.000), and was not correlated with the choline content (rs = 0.088, P = 0.326). Conclusion: The ADC value, NAA, choline and choline content changed in the central area of infarction and peripheral area after acute cerebral infarction. The changes of ADC values has a certain correlation with the NAA and lactic acid content.

Objective To assess the efficacy of laryngeal mask airway (LMA) i-gel used in patients undergoing laparoscopic gynecological surgery. Methods Sixty ASA Ⅰ or Ⅱ patients, aged 21-64 yr, weighing 4590 kg, undergoing elective laparoscopic gynecological surgery, were randomized into 2 groups ( n = 30 each):LMA i-gel group (group Ⅰ) and LMA Supreme group (group S) . Mallampatti test was performed before operation in both groups. Anesthesia was induced with target-controlled infusion of propofol (target plasma concentration 2.5-4.0 μg/ml) and remifentanil (target plasma concentration 3-6 ng/ml) . After the patients lost consciousness, rocuronium 0.6 mg/kg was given to facilitate the LMA insertion. LMA i-gel and LMA Supreme were inserted in I and S groups respectively. A gastric tube was inserted through the drain tube of the LMA. In group S the air was injected into the cuff to make intracuff pressure reach 60 cm H2O after successful LMA insertion. The LMA placement time, the number of attempts of LMA insertion, the number of attempts of gastric tube placement, the airway sealing pressure, the parameters of hemodynamics and ventilation, and complications (sore throat, odynopliagia,hoarseness) were recorded. The fiberoptic laryngoscopy scores were assessed after successful LMA placement. The anesthesia time and recovery time were also recorded. Results There was no significant difference in the anesthesia time, recovery time, LMA placement time, fiberoptic bronchoscopy scores and the parameters of hemodynamics and ventilation between the two group. The success rates of LMA and gastric tube placement were 100% in both groups. The airway sealing pressure was significantly higher, while the incidence of sore throat and odynopliagia was significantly lower in group I than in group S. Conclusion LMA i-gel provides adequate ventilation during operation with fewer complications and can be used effectively for gynecological laparoscopic surgery.

Objective To evaluate the relationship between carotid artery plagues and the clinical risk factors in patients with diabetes mellitus type 2 by observing the ultrasonic characteristics of carotid atherosclerosis plagues.Methods The ultrasound results of carotid arteries in 665 patients with diabetes mellitus type 2 from January 2009 to December 2011 in Beijing Amery General Hospital were retrospectively analyzed.The location and quantity of carotid atherosclerotic plaques were observed .The relationship between the occurrence and risk factors of diabetes such as hypertension ,family history of diabetes and age were analyzed.Single factor and multi-factor analysis were applied for the related risk factors .Results The ultrasonography showed 405 cases of carotid artery plaques.Most carotid artery plaques were located in the posterior wall of carotid sinus and were hyperechoic ,hypoechoic or isoechoic.The single factor analysis showed hypertension,family history of diabetes and age are risk factors of carotid atherosclerosis (χ2 =42.322,9.682 and 140.658,all P<0.01).Multi-factor logistic regression analysis showed age was an independent risk factor of carotid atherosclerosis in patients with diabetes mellitus type 2.The risk factors scores of patients who were less than 45 years,45-65 years and over 65 years of age were (3.50 ±1.16)scores,(3.46 ±0. 92)scores and (3.21 ±0.88)scores,and the difference was statistically significant (F=5.781,P<0.001). There were significant differences between risk factors scores of different age groups ( t =2.084,2.002, 3.786,3.474,2.877 and 2.504,all P<0.05).In patients more than 65 years of age,the risk of atherosclerosis plaques is 28.732 times higher than that of patients less than 45 years of age.After controlling the age factor,the possibility of atherosclerosis plaque increased 1.201 times for each additional one point of score. Conclusions Most carotid artery plaques in patients with diabetes mellitus type 2 are located in the posterior wall of carotid sinus and are hyperechoic ,hypoechoic or isoechoic.Age is an independent risk factor for the formation of carotid plaque.Patients more than 45 years of age should undergo early intervention to avoid the occurrence of major vascular complications .

OBJECTIVETo observe the effects of moxibustion and treadmill exercise on transcutaneous oxygen tension and exercise capacity in lower limbs of peripheral arterial disease (PAD).

METHODSTotally 58 mild-to-moderate PAD patients were assigned to the control group (18 cases), the moxibustion group (20 cases), and the treadmill exercise group (20 cases) by random digit table. Patients in the control group received conventional drug therapy for 12 weeks. Patients in the moxibustion group and the treadmill exercise group additionally received moxibustion [at Zusanli (ST36), Sanyinjiao (SP6), Yongquan (KI1)] and treadmill exercise respectively, once per day, 5 times per week for 12 weeks in total. Ankle-Brachial Index (ABI) , transcutaneous oxygen tension (TcPO₂), 6-min walking test (6MWT), and walking impairment questionnaire (WIQ) were assessed before and after treatment.

RESULTSCompared with the control group and the same group before treatment, there was no statistical difference in ABI in the moxibustion group and the treadmill exercise group (P > 0.05). But TcPO₂, 6MWT, and WIQ were obviously elevated (P < 0.01). Besides, 6MWT and WIQ assessment of the treadmill exercise group were better than that of the moxibustion group (P < 0.01) after intervention.

CONCLUSIONMoxibustion and treadmill exercise could improve the exercise capacity and TcPO₂of lower limbers in PAD patients.

Exercise Test ; Exercise Therapy ; Exercise Tolerance ; Humans ; Lower Extremity ; physiopathology ; Moxibustion ; Oximetry ; Oxygen ; blood ; Peripheral Arterial Disease ; therapy ; Surveys and Questionnaires ; Walking

OBJECTIVE: To optimize the extraction technology of ardicrenin from Ardisia crenata.METHODS: The content of ardicrenin was determined by RP-HPLC. The extraction technology was optimized by orthogonal experiment with the extraction rate of ardicrenin as evaluation index and with the volume fraction of ethanol,the extraction temperature,extraction time and extraction times as factors.RESULTS: The optimum extraction technology was determined as follows: 80%ethanol was used as solvent;the extraction temperature was 80 ℃;the extraction time was 20 min and the extraction was conducted for 3 times.The extraction rate of ardicrenin was 6.04%.CONCLUSION: The optimized extraction technology is feasible and reproducible,and it provides theoretical basis for mass extraction of the ardicrenin from A.crenata.

Objective To study the effect of expansion ex vivo on the hematopoietic reconstitution of umbilical cord blood (UCB) hematopoietic cells, and to find a probable way of UCB transplantation (UCBT) to be used on adults. Method Three groups of UCB hematopoietic cells were transplanted to lethally irradiated BALB/C mice: fresh cells, a half part of fresh cells and a half part of cells expanded for a week, cells expanded for a week. Results All the three groups of cells could reconstitute hematopoiesis. The cells in the latter two groups could more rapidly accelerate the recovery of early-period hematopoiesis. The death rate of the mice in second group was the lowest among three groups. Conclusion The way transplanted with a half part of fresh UCB hematopoietic cells and a half part of UCB hematopoietic cells ex vivo expanded for a week may be a probable one for adult UBCT.

Prostate cancer (PCa) incidence is increasing in China.Because of the limited clinical symptoms,early detection of PCa is critical and difficult.Current clinical diagnostic methods for localizing PCa including both the conventional anatomic imaging and histological techniques have many problems.Enormous novel PCa specific molecular probes have been evaluated.Those probes have substantially improved the diagnostic accuracy and specificity of PCa.This review briefly introduces the conventional noninvasive imaging techniques for PCa diagnosis.Nuclear medicine probes that have great clinical value for PCa molecular imaging are also discussed.

Objective To investigate if lidocaine can alleviate the behavioral disorder, learning and memory dysfunction and neuropathological lesions induced by cerebral microsphere embolism. Methods Sixty-five healthy male Wistar rats weighing 210-270 g were randomly divided into 5 groups: 2 microsphere groups (n= 14 each) , 2 lidocaine pretreatment groups (n=12, 13) and control group (n= 8) . The animals were anesthetized with isoflurane, 60% nitrous oxide and O2 . In the microsphere groups 600 or 900 microspheres were injected into right internal carotid artery. In the lidocaine pretreatment groups a bolus of lidocaine 1.5 mg?kg-1 was given via femoral vein 30 min before microsphere injection followed by lidocaine infusion at 2 mg?kg-1?h-1 until 1h after microsphere injection. In control group vehicle without microsphere was injected. The behavior score was recorded every day from the 1st to 7th day after microsphere injection. The Morris water maze test was performed 3 times a day starting from the 8th to 12th day after microsphere injection. The animals were sacrificed after the last water maze test. Brains were removed for microscopic examination of the hippocampus CA1 region.Results (1) The behavioral scores (0 = no typical signs, 9 = worst results) decreased with time after embolism in all groups and were significantly higher in the microsphere and lidocaine groups than in control group. They were significantly higher in microsphere group 2 than in microsphere group 1 and the two lidocaine groups (P