Apoptosis may play an important role in organ development and balance maintenance, which is beneficial to clear away the abnormal cells. Some teratogens may affect the key apoptotic genes expression, including the genetic defects, the nutritional, and the environmental factors, etc. Apoptotic dysfunction would interfere with the bodys normal development and result in deformity.

Agranulocytosis ; chemically induced ; Antineoplastic Agents, Phytogenic ; administration & dosage ; adverse effects ; Antineoplastic Combined Chemotherapy Protocols ; adverse effects ; therapeutic use ; Camptothecin ; administration & dosage ; adverse effects ; analogs & derivatives ; therapeutic use ; Colorectal Neoplasms ; drug therapy ; pathology ; Diarrhea ; chemically induced ; Drug Resistance, Neoplasm ; Fluorouracil ; adverse effects ; therapeutic use ; Humans ; Leucovorin ; adverse effects ; therapeutic use ; Neoplasm Staging ; Survival Analysis

In recent years, tumor has become one of the major diseases that endanger human health. It is of great significance to explore the pathogenesis of tumors in the prevention, diagnosis, treatment and prognosis of tumors. microRNA (miRNA) is a class of small non-coding RNA, which is involved in post transcriptional processing, epigenetic inheritance, cell growth and individual development and other important life activities. miRNA, charactered by oncogenes and anti-oncogenes function, participates in the development, invasion and metastasis of tumors. Telomeres, located at the end of chromosomes, are closely related to the malignant transformation of cells and the aging of human. To understand the pathogenesis of tumors and to provide a novel direction for the diagnosis, treatment and prognosis of tumors, the role and the relationship of miRNA and telomere in tumors are reviewed in this paper.

BACKGROUND:An appropriate biomaterial can be directly combined with autologous or al ogeneic skin cel s to construct tissue-engineered skin, which can accelerate skin repair after transplantation onto the skin wounds. It is a good idea to solve the deficiency in skin sources. OBJECTIVE:To study the biological characteristics of the complex with bone marrow mesenchymal stem cel s and to investigate the application of this complex in skin defect repair. METHODS:Twenty-eight Sprague-Dawley rats, SPF grade, were randomized into two groups (n=14 per group). By adjustment of temperature, time, pressure and area of NC perm instrument, scald models were made in rats. Rats in the treatment group were given the repair using composite bone marrow mesenchymal stem cel s, while those in the control group were given vaseline gauze repair. Repair effects were compared between two groups. RESULTS AND CONCLUSION:(1) After 72 hours of culture, the composite bone marrow mesenchymal stem cel s smal with round shape distributed dispersedly. After 5 days of culture, the cel s began to stretch and the cel morphology became unstable. After three passages, the cel morphology became stable. The results of antigen identification showed the expression of CD44 and CD29 but the low expression of CD45 and CD34 in bone marrow mesenchymal stem cel s. (2) Twenty-eight days after repair, there was no obvious scar on the wound surface of the treatment group, but a little shrinkage and obvious scar stil existed in the control group. Moreover, in the control group, the epidermal layer of the skin was relatively thick, and the connection between the basal layer and the dermis was unsatisfactory. In the treatment group, obvious epidermal cel stratification, neat arrangement, and tight connection between the epidermis and dermis were observed. In summary, bone marrow mesenchymal stem cel s are a special class of cel s that have pluripotent ability and are more readily available. These cel s are the preferred target cel s for skin defect repair to promote early healing of the skin and improve blood circulation defect site, which are confirmed to have high clinical value.

BACKGROUND: In recent years, acel ular human cadaveric dermis has been applied for repairing dermal defects and fil ing soft tissues, but this repair material is expensive and difficult to obtain. OBJECTIVE: To explore the repair effect of tissue-engineered acel ular dermis on wound healing of skin ulcer. METHODS: Sprague-Dawley rats were randomly divided into autologous reticular dermis and acel ular dermis groups. The tissue-engineered acel ular dermis was prepared by hypotonic solution, 2.5 g/L trypsin, 0.5% Triton X-100 and PBS solution, and in the meanwhile, a rat model of skin ulcer was established. Then, autologous skin graft and tissue-engineered acel ular dermis were transplanted into the autologous reticular dermis group and acel ular dermal group, respectively. Subsequently, comparative study about the repair effect and relative mechanism between two groups was performed. RESULTS AND CONCLUSION: The tissue-engineered acel ular dermis showed a white and grainy shape at room temperature. And hematoxylin-eosin staining showed that: the internal structure of tissue-engineered acel ular dermis exhibited a dendritic distribution, and the gap between col agen fiber bundles was relatively large. At 2, 3, 4 and 8 weeks after dermal transplantation, the survival rate of skin graft in the acel ular dermis group was significantly higher than that in the autologous reticular dermis group (P < 0.05). And the wound contraction rate in the acel ular dermis group was significantly higher than that of the autologous reticular dermis group at 3, 4 and 8 weeks after dermal transplantation (P < 0.05). In addition, the average diameter and clearance rate of col agen fibers in the normal skin around wound in the acel ular dermis group were significantly higher than those of autologous reticular dermis group (P < 0.05). To conclude, it is relatively simple to prepare tissue-engineered acel ular dermis under mild conditions, and the prepared tissue-engineered acel ular dermis presents a regular shape. Moreover, this acel ular dermis achieves desired outcomes in repairing dermal defects, which can promote wound healing by reducing the intradermal DNA residual and significantly improving some biomechanical properties in vivo.

0.05). The movement and invasion ability were evidently inhibited in AQP1-siRNA groups compared with those in blank control groups and negtive control groups [(36? 3), (23?4)/HP vs(70?5),(65?4)/HP and(72 ?4),(69?4)/HP, respectively, P0.05).CONCLUSION The knock down of AQP1 by RNAi have no effect on the adhesion ability of Hep-2 cells, whereas could prevent the movement and invasion ability of laryngocarcinoma cells, which may be related with the metastasis of laryngeal carcinoma.

Antineoplastic Combined Chemotherapy Protocols ; administration & dosage ; therapeutic use ; Cyclophosphamide ; administration & dosage ; Doxorubicin ; administration & dosage ; Humans ; Lymphoma, B-Cell ; classification ; drug therapy ; therapy ; Prednisone ; administration & dosage ; Stem Cell Transplantation ; Vincristine ; administration & dosage

Objective To investigate the suitable therapeutic schedule for Stanford B aortic intramural hematoma associated with calcification. Methods During the period from March 2009 to March 2012, a total of 15 patients of Stanford B aortic intramural hematoma with calcified plaque were admitted to authors’ hospital. The diagnosis was proved in all patients by CT angiography of the entire aorta with a 64-row CT scanner. Of the 15 patients, death occurred in one, thoracic endovascular aortic repair (TEVAR) treatment was adopted in 7 and conservative therapy was carried out in 7. All the patients were followed up for one year. CT angiography was employed at 3, 6 and 12 months after the treatment to evaluate the therapeutic results. Results One patient died of acute myocardial infarction after admission to hospital when the long-tem use of antiplatelet drugs was stopped. Seven patients received TEVAR treatment and the remaining 7 patients were treated with strict conservative therapy, and all these patients were asymptomatic at the time of discharge. During the follow- up period, CT angiography performed at 3, 6 and 12 months after the treatment showed that the intramural hematoma lesions gradually shrank or were absorbed in 13 patients, and the patients were asymptomatic. The remaining one patient was lost in touch. Conclusion The key to treat Stanford B aortic intramural hematoma with calcification is to prevent deterioration of the lesion. While strict conservative treatment is kept on, TEVAR should be promptly carried out for patients who need to take antiplatelet drugs over a long period of time, for patients whose clinical symptoms are not improved, for patients in whom the relived symptoms recur, and for patients whose CT angiography shows that the penetrating aortic ulcer becomes worse.

Objective To explore the effect of conservative treatment for Stanford B aortic intramural hematoma without calcified plaque and the midterm follow-ups.Methods Clinical data of 20 patients with Stanford B aortic intramural hematoma without calcified plaque which were confirmed by CTA of the whole aorta were retrospectively analyzed.There were 12 males and 8 females,with the age of 35～ 52 years.All of them received pharmacotherapy and psychotherapy.Results All the 20 cases were asymptomatic after conservative medical treatment when they discharged,and were followed up whit imaging tests.They all lived without symptoms during the follow-up period.The pleural fluid on the left side was disappeared in 11 cases after 3 months and the aortic intramural hematoma was absorbed in 13 cases after 6 months.After 1 year there were total 18 cases without aortic intramural hematoma,while The thickness and scope of the hematoma were reduced significantly in the other 2 cases.Conclusion It' s ease to find whether the patient with Stanford B aortic intramural hematoma got calcified plaque or not; the conservative treatment for Stanford B aortic intramural hematoma without calcified plaque has satisfactory clinical effect in midterm follow-ups.

Objective To investigate the control status of glycosylated hemoglobin (HbA1c) in the middle aged and elderly patients with type 2 diabetes mellitus (T2DM) in Lanzhou communities and analyze its influencing factors.Methods A total of 1 120 individuals having diagnosed T2DM aged 40-75 years in Lanzhou communities were selected.Questionnaire was conducted.Height,weight,blood pressure and lipid were examined.Body mass index (BM1) was calculated.The index were analyzed.Results The patients with HbA1c less than 7.0％ were 453 cases (40.4％,453/1 120),and HbA1c more than or equal to 7.0％ were 667cases (59.6％,667/1 120).The target rate of HbA1c in the patients receiving life style,one oral antidiabetic drug,more oral antidiabetic drug,insulin therapy were 49.2％(94/191),45.7％(182/398),41.5％(88/212) and 27.9％(89/319),respectively.The target rate of HbA1c were 38.6％(192/298),32.2％(49/152),32.7％(49/150),27.0％(85/315) in the patients overweight or obese,with macro or micro vascular complication,and 60.0％ (12/20),44.4％(200/450),41.6％(404/970),45.7％(368/805) in the patients without overweight or obese and macro or micro vascular complication,and there were significant differences (P ＜ 0.01 or ＜ 0.05).Multivariate Logistic regression analysis showed that the extension duration of diabetes,increased BMI,combining with macro or micro vascular complication and gradual strengthening antidiabetic treatment were independent risk factors for HbA1c target (P ＜ 0.05).Conclusions Current status of HbA1c control in the middle aged and elderly patients with T2DM in Lanzhou communities is not optimistic,which is affected by many factors,suggesting that professional education on management should be further strengthened.And optimal treatment programs and individualized comprehensive treatment are needed for most patients to improve glycemic control.