3.Changes of metabolic syndrome related indexes before and after blood donation in blood donors
International Journal of Laboratory Medicine 2015;(5):636-637
Objective To investigate the significance of blood donation in preventing metabolic syndrome (MS)by analyzing the changes of the blood pressure and the related biochemical indexes before and after blood donation in the blood donors with high risk of MS.Methods The blood specimens of 102 blood donors,aged 45 -55 years old with the systolic blood pressure of 110 -140 mmHg and the diastolic blood pressure of 75 -90 mmHg,the body mass index> 24 kg/m2 ,were selected as the subjects.The blood pressure,heart rate,iron reserves,blood glucose,blood lipids,and the other indicators were determined before blood donation. All the above indicators were tested again on 42 d after blood donation.Results The blood pressure,iron reserves,blood glucose and LDL/HDL ratio after donation showed significant changes compared with before blood donation(P <0.05 ).Conclusion The changes of the MS related blood indexes exist before and after blood donation,blood donation has a certain significance for preven-ting MS in the blood donors with high risk of MS.
4.Study on the correlation between social emotion and temperament in infants and toddlers
Journal of Clinical Pediatrics 2013;(9):862-865
Objectives To study the correlation between social emotion and temperament in infants and toddlers. Me-thods The Brief Infant-Toddler Social and Emotional Assessment (BITSEA) and Toddler Temperament Questionnaire (TTQ) were used to evaluate the social emotional problems and temperament of 657 infants and toddlers 1 to 3 years old. Results The temperament dimensions were correlated with behavior and competence factors. The infants and toddlers in dififcult type group had more behavior problems or deifcits/delays in competence than those in easy type group (χ2=26.49, 10.48, P<0.01). Conclu-sions Infants and toddlers with negative temperament traits have more behavior problems and weaker social emotional compe-tences. The temperament should be considered in dealing with emotional problems.
5.Chondrogenesis of adipose tissue-derived stem cells induced by auricular chondrocytes from microtia in vivo
Chinese Journal of Tissue Engineering Research 2017;21(21):3312-3319
BACKGROUND:Due to quantity and quality deficiencies, chondrocytes from microtia are difficult to act as seed cells to construct an ear cartilage scaffold with the normal human auricle size. OBJECTIVE: To test the hypothesis that auricular chondrocytes from microtia can promote chondrogenic differentiation and chondrogenesis of human adipose tissue-derived stem cells (ADSCs) at non-chondrogensis sitein vivo, which is the preparatory work for preparation of human tissue-engineered auricle cartilage scaffold. METHODS: Human ADSCs at passage 3 and auricular chondrocytes at passage 2 were mixed at a ratio of 7:3 and 5.0×1010/L mixed cells were suspended in 0.2 mL of 30% Pluronic F-127, and then the mixture was injected subcutaneously into Balb/c nude mice as experimental group. Auricular chondrocytes or ADSCs at the concentration of 5.0×1010/L were mixed with 0.2 mL of Pluronic F-127 and injected respectively as positive and negative control groups. 1.5×1010/L auricular chondrocytes were mixed and injected as low-concentration chondrocyte control group. All specimens were collected at the 8th week post-injection. Newborn tissues in nude mice were taken out for morphological examination, wet weight measurement, determination of glycosaminoglycans, histological and immunohistochemical staining. RESULTS AND CONCLUSION:The wet weight of specimens in the experimental group was over 80% of that in the positive control group, and the wet weight of specimens in the low-concentration chondrocyte control group was less than 30% of that in the positive control group. The average wet weight and glycosaminoglycan content were significantly higher in the experimental and positive control group than in the negative control and low-concentration chondrocyte control groups (P < 0.05). In all the groups except for the negative control group, mature cartilage lacunas could be observed by histological staining and collagen type Ⅱ could be detected for expression by immunohistochemistry to different extents. In the low concentration chondrocyte control group, cartilage lacunas were incompact and inhomogeneous, and the extracellular matrix was slightly stained. In the negative control group, mature cartilage lacunae and collagen type Ⅱ could not be detective. To conclude, auricular chondrocytes from microtia can promote chondrogenic differentiation and chondrogenesis of ADSCs at the non-chondrogenesis sitein vivo.
6.Clinical study of chemoradiation therapy in rectal cancer
China Oncology 2001;0(05):-
Rectal cancer is a common malignant tumor in the gastrointestinal system.For the patients with resectable but high-risk rectal cancer,neoadjuvant and adjuvant chemoradiation plus surgery may improve local control.Compared to adjuvant chemoradiation,neoadjuvant chemoradiation showed the advantage of sphincter preservation.When the goal of preoperative chemoradiation was sphincter preservation,conventional radiotherapy was recommended.Through the analysis of the patients with 5-year survivals,the radiation had a similar impact on the survival as the chemotherapy alone for the patients with the intermediate risk factors.The impact on local control need to be further investigated before recommending chemotherapy alone for those subset patients.Advances in preoperative irradiation,postoperative irradiation and the comparison of preoperative irradiation with postoperative irradiation are also reviewed in this article.
7.Effects of Drug Checking on the Occurrence of Errors in the Drug Treatment
China Pharmacy 2001;0(07):-
OBJECTIVE:To explore the ways of preventing errors in the process of drug treatment.METHODS:Related overseas literatures were consulted;causes of errors in the drug treatment were analyzed,which combined with prac-tice.RESULTS&CONCLUSIONS:Errors can be found effectively and reduced by establishing independent checking and error reporting system.
8.Application of Kinesio Taping in Children with Cerebral Palsy (review)
Chinese Journal of Rehabilitation Theory and Practice 2015;21(10):1168-1172
Usually individualized comprehensive treatments are applied to children with cerebral palsy. Among them, Kinesio taping is a simple treatment compared to orthosis, which allows greater rang of motion (ROM) and can more easily be accepted by patients, however, its effectiveness is controversial. This article introduced the mechanism that mainly includes relieving pain, improving joint mobility, increasing strength, relieving edema and increasing proprioception. The application of it in upper limbs, lower limbs and trunk of children with cerebral palsy was discussed. Finally, the advantages and limitations of Kinesio taping, suitable types of cerebral palsy and the therapeutic evaluation were analyzed.
9.ATRA combined with IFN-α2a promotes iodine uptake of FTC-133 follicular thyroid carcinoma cells
Zhen ZHAO ; Guohua SHEN ; Huawei CAI
Journal of Medical Postgraduates 2016;29(9):910-912
Objective Some drugs can effectively improve the uptake of 131 I in dedifferentiated thyroid cancer .This study was to investigate the effect of all-trans retinoic acid (ATRA) combined with interferon-α2a (IFN-α2a) on the iodine uptake and re-tention rate of FTC-133 follicular thyroid carcinoma cells . Methods FTC-133 cells were cultured in the presence of 2 μmol/L AT-RA and/or 1500 IU/mL IFN-α2a for 72 hours.The the cells were collected for measurement of the uptake and retention rate of Na 125 I. Results The Na125 I uptake of the FTC-133 cells was significantly increased after 72-hour incubation with 2μmol/L ATRA and 1500 IU/mL IFN-α2a (2423.1 ±237.5) as compared with those of the control (1279.5 ±102.8), ATRA (1438.2 ±149.6), and IFN-α2a groups (1355.3 ±198.4) (P<0.05).Statistically significant differences were observed in the retention rate of Na 125I in the FTC-133 cells at different time points in the each of the blank control , 2μmol/L ATRA, 1500 IU/mL IFN-α2a, and combination groups (P<0.01), but not in that of Na125I among these groups (P<0.05). Conclusion ATRA in combination with IFN-α2a can promote the Na125 I uptake but cannot improve the Na 125 I retention rate in FTC-133 cells.
10.Research advancement of pro-apoptotic mechanisms of GRIM-19 and its related proteins
Guohua SHEN ; Huawei CAI ; Zhen ZHAO
Journal of Medical Postgraduates 2015;(3):303-307
Cancer development is a complex process that involves multiple genetic changes and multiple signaling pathways . Recent findings show that the GRIM-19 is a novel apoptosis regulation gene , and its gene mutations and loss of protein expression have been observed in many tumor types such as urinarysystem tumor , digestive system neoplasm , which are closely related to cancer devel-opment.Thus, GRIM-19 may be a potential target for gene therapy .Pro-apoptotic mechanisms of GRIM-19 and its related proteins such as STAT3,GW112,p16INK4aare overviewed in this article.