Child ; Humans ; Inflammation ; Neoplasms, Muscle Tissue ; Ureteral Neoplasms

The levels of c-fos mRNA in U937 cell line were determined with in situ hybridization.Theresults showed that TPA induced the expression of c-fos gene with a relationship of dosage-ef-fect.It was also proved that GM-CSF,TNF,IL-9,IL-3 and IL-6 increased the expression of c-fos gene.

Background Epithelial-mesenchymaltransition (EMT)isamajorcontributortothe pathogenesis of posterior capsular opacification(PCO).Kruppel-like factor 6 (KLF6) is a zinc finger protein,which can be stimulated by high glucose in proximal tubule cells and involved in transforming growth factor beta (TGF-β)induced EMT of diabetic nephropathy.ObjectiveThis study was designed to investigate the effect of high glucose on the expression of KLF6 and its target genes( TGFB1,TGFBR1,COLIA1,HSP47) in human lens epithelial cells (LECs).MethodsHuman LECs(SRA01/04) were cultured and exposed to different concentration of glucose.The expressions of KLF6 mRNA and protein were analyzed by real time polymerase chain reaction( real time PCR) and western blot after treatment with high glucose.The expressions of KLF6 target genes were analyzed by real time PCR to evaluate the EMT of SRA01/04 cells.ResultsCompared with the control group(5.5 mmol/L),the relative mRNA levels of t-KLF6 and wt-KLF6 in SRA01/04 treated with high glucose(22.2,44.4,66.6 mmol/L) increased obviously (F =72.53,42.02,P＜0.01 ).Then,the concentration of 22.2 mmol/L was used in the next experiments.The relative mRNA levels of t-KLF6 and wt-KLF6 increased to the peaks after treatment with high glucose for 12 h,and began to decrease after 24 h until lower levels after 48 h ( F =100.12,125.52,P ＜ 0.01 ).Western blot showed that the expression of KLF6 protein was also upregulated by high glucose treatment.With the promotion of the expression of KLF6 gene,the relative mRNA levels of TGFB1,TGFBR1,COLlAl and HSP47 of treated cells also respectively increased after treatment for 12 h,and began to decrease after 24 h until nearly at the levels of the control groups after 48 h( F=6.73,162.35,64.39,12.05,P＜0.05 ).ConclusionsIt was concluded that high glucose induced the expression of KLF6 in human LECs,and KLF6 transiently stimulated the expression of target genes TGFB1,TGFBRl,COLlAl and HSP47 which were mainly involved in the mechanism of EMT.

Objective To study the angular characteristics of facial profile in normal Han Chinese adults of Guangdong natives and the value of measurement methods adapted in this project. Methods One hundred and fifty Han Chinese adults (ratio of male to female,1∶1)were randomly chosen and parameters for computer aided angular analysis of facial soft tissues included:lateral angles:∠Ns Prn Pgs;∠Ns Prn GsPgs;∠Ns Prn Sn; As Ns Bs;∠Ns Sn Pgs;∠Gs Ns Prn;∠Cm Sn Ls and ∠Li Bs Pgs. front angles: ∠Zyr Rc Mes; ∠Zyl Lc Mes; ∠Exr Exl Sn; ∠Exl Exr Sn; ∠Exr Sn Exl; ∠Enl Enr Sn; ∠Enr Enl Sn; ∠Enr Sn Enl; ∠Sn Chr Chl; ∠Sn Chl Chr and ∠Chr Sn Chl.Results Lateral angles ∠Ns Prn Sn and ∠Li Bs Pgs,in male were larger significantly than that those in female, but ∠Gs Ns Prn in male was smaller significantly than that in female. Front angles,∠Zyr Rc Mes and ∠Zyl Lc Mes,in male were larger significantly than those in female.Conclusion Angular analysis plays a unique role in the measurement of facial soft tissue profile. There is a difference of facial soft tissue profile between male and female Han Chinese.

Obiective To study the clinical characteristics and therapeutic measures of the antibiotic associated diarrhea (AAD) in children with severe bacterial pneumonia. Methods The information of antibiotic use history in children with severe bacterial pneumonia from January 2013 to June 2013 was retrospectively analyzed. Results The incidence rate of AAD was 70.59%in children with severe bacterial pneumonia. According to the frequency, the third-generation cephalosporins, penicillin and carbapenems were the major causes of AAD. The percentage of the age≤3 y, the duration of antibiotic usage>7 d and the number of antibiotics≥2 were significantly higher in AAD children than in non-AAD children (χ2=89.81~119.45, P<0.001). All the patients were improved after cessation or change of antibiotics. Conclusions The incidence of AAD in children with severe bacterial pneumonia is high, which is related to the age, the duration of antibiotic usage and the combination use of antibiotics.

Humans ; Postural Orthostatic Tachycardia Syndrome

Objective To study the expression of telomerase and its possible clinical significance in patients with condyloma acuminatum,bowenoid papulosis,Bowen's disease and vulvar squamous cell car- cinoma.Methods The human telomerase reverse transcriptase (hTERT) was detected by immunohisto- chemistry technique in all patients.Histopathological analysis was done in patients with bowenoid papulosis or Bowen's disease.Results There was a significant difference in the expression of hTERT among normal tissue,condyloma acuminatum and squamous carcinoma,and the expression rate increased successively from normal control to squamous carcinoma.In bowenoid papulosis,the expression of bTERT was significantly higher as compared to that in condyloma acuminatum,significantly lower as compared to that in squamous carcinoma,and was as the same as that in Bowen's disease.Histopathologically,the degree of cell atypia was significantly higher in Bowen's disease than that in bowenoid papulosis.Conclusions The expression of telomerase increases successively from normal skin,benign tumors to malignant carcinomas,which sug- gests that the expression of telomerase may play an important role in both cell proliferation and immortaliza- tion.

Objective To investigate the potential effect of Lactobacillus acidophilus (L. acidophilus) on prevention and treatment of neonatal mice infected with human rotavirus (HRV). Methods Sixty 4-day-old kunming mice were randomly divided into control group. HRV infected group, L. acidophilus pretreated group (treated before HRV infection ) and L. acidophilus treated group(treated after HRV infection). The manifestation and pathological changes in small intestine of neonatal mice were observed. The HRV antigen in the feces and intestines were measured by ELISA and fluorescent-focus assay, respectively. Results The severity and duration of diarrhea as well as mortality in L. acidophilus pretreated group and treated group were lower than those in HRV infected group. The duration of HRV-antigens shedding following infection was considerably prolonged in HRV infected group compared to that in L. acidophilus pretreated group and treated group. Furthermore, decreased expression of HRV antigen and little pathological changes in intestinal mucosa were found in L. acidophilus pretreated group and treated group when compared with HRV infected group. Conclusion L. acidophilus may be used as an alternative approach for the prevention and treatment of neonatal mice infected with HRV.

Objective To investigate the screening state and cause of neonatal congenital hypothyroidism(CH) in Henan province.Me-thods Three hundred and thirty-eight thousands no-choice newborns in the 156 hospitals in Henan province from Jan.1998 to Dec.2004 were received screening.The heel peripheral blood samples were taken and the levels of thyroid-stimulating hormone(TSH) were mea-sured by time-resolved fluoroimmunoassay.Positive newborns were recalled and draw out 2 mL venous blood.The levels of TSH,triiodothyronine(T3) and thyroxine(T4) in serum were detected by direct chemiluminescence.The abnormal criterion was that the level of T3,T4 was under the normal range and the level of TSH was above the normal range.Meanwhile their growth and development,intelligence quotient,related medical examination and family histories were investigated in order to find etiological factors and high risk factors.Results The average coverage rate was 5.93% of neonatal screening for CH in Henan province.One hundrend and nine infants were diagnosed CH.The attack rate was about 0.032%.The site and growth of thyroideas of 109 cases with CH were all normal.There were higher morbidity for pregnant women with unhealthy condition or the families with hypertension,diabetes,deformity or dysnoesia.Conclusions Etiology of newborns with CH in Henan province may be not relate with congenital absence and dystopia of thyroidea,but relate with dyssynthesis and receptor deficiency of hormone.And scientific reference is offered for the further study.

Objective: Bladder cancer is the ninth cause of cancer deaths and has high recurrence rate after resection of the tumors. Cystoscopy is the current most accurate method for investigating the bladder abnormalities. However, it is expensive, uncomfortable and invasive. It is possible to induce bleeding, urinary-tract infection and even puncture of bladder. Advances in medical imaging and computer technologies make virtual cystoscopy a potential alternative. Methods: Computed tomography (CT) and magnetic resonance imaging (MR/) are the preferred imaging modalities for virtual cystoseopy to get clear structural images or (and) functional images of the bladder. The boundary of bladder is segmented manually or automatically, and then the bladder is reconstructed and displayed by surface rendering. Useful features are extracted from the images and expressed o nthe reconstructed bladder to provide more valuable diagnosis information for doctors. Results: Comparing with conventional cystuscopy, virtual cystuscopy is noninvasive, more convenient, flexible and can provide more useful diagnosis information as well. Conclusions: Virtual cystoscopy is a promising method of detection and reexamination of bladder cancer. So far, more researches are needed for the virtual cystoscopy before clinical application. It clinical and commercial value are under investigation.